褐牙鲆(♀)、夏鲆(♂)及其杂交子一代线粒体16S rDNA序列遗传特性的初步研究

以线粒体16S rRNA基因部分片段为代表研究雌褐牙鲆Paralichthys olivaceus、雄夏鲆P. dentatus及其杂交子一代间线粒体DNA的遗传特性。母本与父本序列差异较大,属种间差异,在590个位点中有28个变异位点,而且全部为简约信息位点。使用同一对引物扩增亲本与杂交子代,在杂交子代中未检测到父本的线粒体DNA类型,11个处于两种生长阶段的杂交样本仅检测到一种单倍型,而且与母本的一个单倍型为同一种,表明褐牙鲆与夏鲆杂交线粒体DNA遵循母系遗传规律。     

Variations of antioxidant efficiency and presence of endosymbiotic diatoms in the Antarctic porifera Haliclona dancoi

The role of endosymbiotic diatoms as pro-oxidant stressors in porifera has been investigated in the Antarctic sponge Haliclona dancoi in which the presence of diatoms is influenced by marked seasonal variations during the austral summer. Both chlorophaeopigments and frustules were absent in sponge tissues sampled in early November at the beginning of the summer and increased from the mid of December with slightly shifted temporal trends. The efficiency of antioxidant defenses in the sponge showed a marked response to symbionts with clearly enhanced values corresponding to the peak of diatoms.     

Measurement of DNA single-strand breaks in gill and hemolymph cells of mussels, Mytilus sp., collected on the French Atlantic Coast

DNA single-strand breaks were measured by the comet assay in both gill and hemolymph cells of mussels collected in 3 sampling areas of the French coast (Pointe du Castelli, Pen Bron and Saint-Nazaire Harbour). Whole mussel tissue samples were also collected for the chemical determination of PAH, PCB and heavy metal concentrations. In mussel, a higher level of DNA strand breaks was measured in gill than in hemolymph cells (p < 0.01). Despite a factor of contamination from 2 to 3 between sites, no difference in the extent of mussel DNA strand breaks was shown between sampling locations (p > 0.05), questioning the sensitivity of the assays used in biomonitoring studies.     

玉筋鱼养活实验——水底铺沙法

作者在进行有关海洋食物链能流转换实验研究中,遇到了玉筋鱼(Ammodytesperonatus)养活的困难,致使该项实验中途停顿,因此,进行了玉筋鱼的养活实验。本文阐明了在人工条件下养活玉筋鱼的过程和方法。1　玉筋鱼的养活实验1991年4月9日,从青岛近海捕获玉筋鱼数10尾,放入玻璃水族缸内(60L)暂养,微充气,并投喂适量卤虫(ArtemiasinicaC.)幼体,投喂密度为10～20个/ml,12h后,随机取样,观察其胃含物,结果表明,玉筋鱼几乎不摄食。在此同时,进行其行为观察,发现玉筋鱼多在水的中上层集群,辗转游动,即便在黑暗中,也不停止,且其头部常常撞击缸壁,3d后,…     

Oxidative stress in ecotoxicology: from the analysis of individual antioxidants to a more integrated approach

An integrate approach will be discussed for investigations on oxidative stress in xenobiotic toxicity. While the analysis of individual antioxidants is useful for their sensitivity and to understand the mode of action of a stressor, the integration with the analysis of the total antioxidant capacity provides a more holistic assessment of the overall biological significance of such variations. TOSC has a greater predictive value on the health condition of the organisms and allows to discriminate the different role of specific ROS in oxidative stress syndrome.     

一个简单食物链的能流实验

食物链能量转换是生态系统的主要功能之一。海洋生物生产就是海洋食物链能量流动的一种表现形式。它把来自以藻类为主的植物性生产，通过被食－取食多级转换，形成了贝、虾、鱼等各级动物性生产，这就为人类提供了丰富的渔业资源。海岸带水域是我国目前海洋开发的主攻目标，揭示这个海区中食物链能流转移过程，对其经济生物生产力估算和开发前景预测有着重要意义。测定自然条件下海洋食物链能流是极其困难的。借助实验结果进而推算，就比较方便。本研究选择我国海岸带水域中属于不同营养级的４个经济种，构成一个简单的人工食物链，即金藻（…     

大西洋扇贝贝壳生长年轮的氧同位素研究

两只活的大洋扇贝Placopecten magellanicus分别于1983和1985年采自加拿大诺瓦·斯科舍省布朗斯浅滩,其氧同位素记录展示出一种年度性的循环,并与由环境海水的实测温度和同位素组成所预计的贝壳同位素组成十分接近。外生长线的位置与这些循环中δ~(18)O的最大值吻合,这说明外生长线于底层海水温度最低时(春季)形成。据测定的δ~(18)O的结果证明,外生长线是年度性形成的,这与生物学证据一致。     

由偏顶蛤贝壳中稳定同位素剖面追溯海水温度记录

本文用在加拿大诺瓦·斯科舍省芬克湾采集到的两只活体偏顶蛤(Modiolus)贝壳上的氧同位素组成重建了过去十年间的底层海水温度记录,它也可作为一种独立的方法来确定贝壳上外生长线的沉积时间。由于对贝壳进行了密集采样(可多至每个贝壳沉积年份取15个样品),贝壳上的δ~(16)O变化反映了芬克湾底层海水的季节性变化。两只贝壳所得的温度剖面符合得很好,说明它们基本上是与真实的环境温度相对应的。本研究证明在海胆疾病爆发时底层海水的温度并没有异常升高,这说明疾病传播的关键因素是带病的生物体,而不是温度。同位素技术也说明了在贝壳外表面上可见的外生长线是年轮性的,它们是在每年冬季最低水温时沉积出来的。