Cytochromes P-450 in cod (Gadus morhua): Effects of induces on regiospecificity of phenanthrene metabolism and immunochemical properties

The metabolism of some xenobiotics can lead to the formation of reactive intermediates with mutagenic/carcinogenic properties. With the carcinogenic PAH these have been identified as bay-region diol-epoxides.¹ Phenanthrene, a non-carcinogenic, bay-region containing model PAH, is metabolised in vivo by bony fish at the proximate bay-region position, whereas mammals and other marine organisms mainly form the K-region metabolite 9,10-dihydro-9,10-dihydroxy-phenanthrene.² We wanted to investigate this difference more closely by studying the regiospecificity of phenanthrene metabolism in vitro both with microsomes from differently pretreated cod and with isolated cytochrome P-450 isozymes from BNF-induced cod.³ Secondly, by preparing antibodies to the major isozyme isolated (called cod P-450c), we investigated the immunochemical properties of the variously treated cod liver microsomes.     

Turkey Lakes Watershed,Ontario, Canada: 40 years of interdisciplinary whole-ecosystem research

The Turkey Lakes Watershed (TLW) study is a federal, interdepartmental study established in 1979 to investigate the effects of acid rain on terrestrial and aquatic ecosystems. The 10.5 km² watershed, located in the Eastern Temperate Mixed Forest on the Canadian Shield, has been the site of multidisciplinary studies on biogeochemical and ecological processes conducted across plot to catchment scales. The whole-ecosystem investigative approach was adopted from the outset and has allowed research to evolve from its original (and continuing) acidification focus to include investigations on the effects of climate change, forest harvesting and other forest ecosystem perturbations. The extensive scientific and support infrastructure allows for collection of a comprehensive data record essential for understanding long-term environmental trends. Data include atmospheric deposition, meteorology, stream hydrology and chemistry, soil, pore and ground water properties, understory and overstory vegetation, lake and outflow physical and chemical properties, and aquatic macroinvertebrate and fish community composition and abundance. These data have contributed to over 400 published research papers and graduate theses. The watershed has also figured prominently in many continent-wide comparisons advancing fundamental watershed theory. The knowledge gained at TLW has influenced pollutant emission and natural resource management policies provincially, nationally and internationally.     

Abrupt deglaciation on the northeastern Tibetan Plateau: evidence from Lake Qinghai

We inferred the climate history for Central Asia over the past 20,000 years, using sediments from core QH07, taken in the southeastern basin of Lake Qinghai, which lies at the northeastern margin of the Tibetan Plateau. Results from multiple environmental indicators are internally consistent and yield a clear late Pleistocene and Holocene climate record. Carbonate content and total organic carbon (TOC) in Lake Qinghai sediments are interpreted as indicators of the strength of the Asian summer monsoon. Warm and wet intervals, associated with increased monsoon strength, are indicated by increased carbonate and TOC content. During the glacial period (~20,000 to ~14,600 cal year BP), summer monsoon intensity remained low and relatively constant at Lake Qinghai, suggesting cool, dry, and relatively stable climate conditions. The inferred stable, cold, arid environment of the glacial maximum seems to persist through the Younger Dryas time period, and little or no evidence of a warm interval correlative with the Bølling–Allerød is found in the QH07 record. The transition between the late Pleistocene and the Holocene, about 11,500 cal year BP, was abrupt, more so than indicated by speleothems in eastern China. The Holocene (~11,500 cal year BP to present) was a time of enhanced summer monsoon strength and greater variability, indicating relatively wetter but more unstable climatic conditions than those of the late Pleistocene. The warmest, wettest part of the Holocene, marked by increased organic matter and carbonate contents, occurred from ~11,500 to ~9,000 cal year BP, consistent with maximum summer insolation contrast between 30°N and 15°N. A gradual reduction in precipitation (weakened summer monsoon) is inferred from decreased carbonate content through the course of the Holocene. We propose that changes in the contrast of summer insolation between 30°N and 15°N are the primary control on the Asian monsoon system over glacial/interglacial time scales. Secondary influences may include regional and global albedo changes attributable to ice-cover and vegetation shifts and sea level changes (distance from moisture source in Pacific Ocean). The abruptness of the change at the beginning of the Holocene, combined with an increase in variability, suggest a threshold for the arrival of monsoonal rainfall at the northeastern edge of the Tibetan Plateau.     

Characteristics of the hepatic microsomal cytochrome P-450 system of the minke whale (Balaenoptera acutorostrata)

The whales are an interesting group in several aspects of science, including evolutionary biology, marine ecology and toxicology. Some of the whale species, including the minke whale, graze at the top of the food chains, where they become susceptible to the accumulation of marine pollutants ingested by organisms at lower levels. The northern stock of the minke whale grazes in Arctic waters in the summer, an area of increasing interest in the development of new oil fields. Studies on the metabolism, disposition and effects of xenobiotics in marine mammals are few. The only reports in the open literature so far have dealt with these questions in seals.^1–3 Although the marine mammals comprise a particularly interesting group in marine ecology and toxicology, no data have to our knowledge been presented on the xenobiotic metabolising enzyme systems of the whales. In this study, components of the microsomal cytochrome P-450 system were measured in liver samples from several females, one male, and two foetal minke whales, caught in the Norwegian whaling season of 1983. Because of the limited number of samples studied we have treated the samples individually and not performed any statistical analysis of the data. The trends discussed below must therefore be considered with this background.     

ON THE SENSITIVITY OF PRECIPITATION FORECASTS TO THE MOIST PHYSICS AND THE HORIZONTAL RESOLUTION OF NUMERICAL MODEL*

The impacts of the enhanced model's moist physics and horizontal resolution upon the QPFs(quantitative precipitation forecasts) are investigated by applying the HIRLAM(high resolution limited area model) to the summer heavy-rain cases in China.The performance of the control run,for which a 0.5°×0.5°grid spacing and a traditional "grid-box supersaturation removal+Kuo type convective paramerization" are used as the moist physics,is compared with that of the sensitivity runs with an enhanced model's moist physics(Sundqvist scheme) and an increased horizontal resolution(0.25°×0.25°),respectively.The results show:(1) The enhanced moist physics scheme(Sundqvist scheme),by introducing the cloud water content as an additional prognostic variable and taking into account briefly of the microphysics involved in the cloud-rain conversion,does bring improvements in the model's QPFs.Although the deteriorated QPFs also occur occasionally,the improvements are found in the majority of the cases,indicating the great potential for the improvement of QPFs by enhancing the model's moist physics.(2) By increasing the model's horizontal resolution from 0.5°×0.5°,which is already quite high compared with that of the conventional atmospheric soundings,to 0.25°×0.25°without the simultaneous enhancement in model physics and objective analysis,the improvements in QPFs are very limited.With higher resolution,although slight amelioration in locating the rainfall centers and in resolving some finer structures of precipitation pattern are made,the number of the mis-predicted fine structures in rainfall field increases with the enhanced model resolution as well.