Gas hydrate saturation from acoustic impedance and resistivity logs in the Shenhu area, South China Sea

During the China’s first gas hydrate drilling expedition -1 (GMGS-1), gas hydrate was discovered in layers ranging from 10 to 25 m above the base of gas hydrate stability zone in the Shenhu area, South China Sea. Water chemistry, electrical resistivity logs, and acoustic impedance were used to estimate gas hydrate saturations. Gas hydrate saturations estimated from the chloride concentrations range from 0 to 43% of the pore space. The higher gas hydrate saturations were present in the depth from 152 to 177 m at site SH7 and from 190 to 225 m at site SH2, respectively. Gas hydrate saturations estimated from the resistivity using Archie equation have similar trends to those from chloride concentrations. To examine the variability of gas hydrate saturations away from the wells, acoustic impedances calculated from the 3 D seismic data using constrained sparse inversion method were used. Well logs acquired at site SH7 were incorporated into the inversion by establishing a relation between the water-filled porosity, calculated using gas hydrate saturations estimated from the resistivity logs, and the acoustic impedance, calculated from density and velocity logs. Gas hydrate saturations estimated from acoustic impedance of seismic data are ∼10-23% of the pore space and are comparable to those estimated from the well logs. The uncertainties in estimated gas hydrate saturations from seismic acoustic impedances were mainly from uncertainties associated with inverted acoustic impedance, the empirical relation between the water-filled porosities and acoustic impedances, and assumed background resistivity.     

利用牙鲆鳃细胞系分离和培养淋巴囊肿病毒

本文利用牙鲆鳃细胞系进行了养殖牙鲆淋巴囊肿病毒的分离及培养 ,并通过电镜对培养细胞中淋巴囊肿病毒的形态及感染循环进行了初步研究。将病鱼的淋巴囊肿组织无菌滤液接种牙鲆细胞系 ,细胞出现了明显的细胞病变 ( Cytopathic effect,CPE)。电镜观察在培养细胞的胞质中有病毒的包涵体 ,胞质中散在 6角形、5角形或圆形的病毒粒子 ,大小为 10 0～ 140 nm之间。在感染细胞的线粒体中也存在大量的病毒颗粒。     

围隔实验中海洋浮游植物对石油烃污染物中正构烷烃的生物富集

本文应用海洋围隔生态实验研究了浮游植物对石油烃污染物生物富集动力学过程 ,提出了“水相差法”测定海洋浮游植物体内石油烃浓度 ,以及包括石油烃挥发和生物生长等影响因素的石油烃生物富集动力学模型 ,并利用非线性拟合技术得到了海洋浮游植物对 0 #柴油 WAF中正构烷烃的生物富集动力学参数 kup,kel和 BCFPOC,结果与 Kováts色谱保留指数具有很好的相关性 ,与利用生物分析平衡法测定的文献数据基本一致。本模型及实验方法简便可靠 ,可在现场实验中广泛应用 ,得到的动力学参数应用于多介质环境模型和生态动力学模型 ,用以研究石油烃污染物中正构烷烃在海洋环境中的迁移变化规律     

Diagnosis of iridovirus in large yellow croaker by PCR

A rapid and sensitive PCR-based method for the detection of the large yellow croaker iridovirus(LYCIV) is described,which involves the amplification of a 295 bp fragment of the LYCIV ATPase gene from DNA isolated from naturally infected fish spleen.Sequencing of LYCIV ATPase gene fragment showed it shared 100% nucleotide sequence homology with the corresponding region of the ATPase gene of red sea bream iridovirus(RSIV) and sea bass iridovirus(SBIV),suggesting that LYCIV was homologous with RSIV and SBIV at least in part of the gemone.The specificity and sensitivity of the PCR procedure were tested on the iridovirus-infected fishes,the expected fragment was detected from spleen DNA samples of infected fishes,whereas no fragments were amplified from healthy fish spleen DNA,white spot syndrome baculoviruses(WSBV) DNA and pseudorabies virus(PRV) DNA.Detection limit of this method was 10^-7 ng positive plasmid DNA containing target sequence,equal to about 100 virions.In the infected experiment,first positive detection(1/4) appeared at Day 3 post-infection,all fish(4/4) tested positive at Day 7,however obvious symptoms were observed at Day 8,so LYCIV infection could be detected prior to the appearance of obvious symptoms.These results indicate that this PCR method could be used for early,rapid and specific detection of LYCIV infection.     

ENSO variability and the eastern tropical Pacific: A review

El Niño-Southern Oscillation (ENSO) encompasses variability in both the eastern and western tropical Pacific. During the warm phase of ENSO, the eastern tropical Pacific is characterized by equatorial positive sea surface temperature (SST) and negative sea level pressure (SLP) anomalies, while the western tropical Pacific is marked by off-equatorial negative SST and positive SLP anomalies. Corresponding to this distribution are equatorial westerly wind anomalies in the central Pacific and equatorial easterly wind anomalies in the far western Pacific. Occurrence of ENSO has been explained as either a self-sustained, naturally oscillatory mode of the coupled ocean–atmosphere system or a stable mode triggered by stochastic forcing. Whatever the case, ENSO involves the positive ocean–atmosphere feedback hypothesized by Bjerknes. After an El Niño reaches its mature phase, negative feedbacks are required to terminate growth of the mature El Niño anomalies in the central and eastern Pacific. Four requisite negative feedbacks have been proposed: reflected Kelvin waves at the ocean western boundary, a discharge process due to Sverdrup transport, western Pacific wind-forced Kelvin waves, and anomalous zonal advections. These negative feedbacks may work together for terminating El Niño, with their relative importance being time-dependent.ENSO variability is most pronounced along the equator and the coast of Ecuador and Peru. However, the eastern tropical Pacific also includes a warm pool north of the equator where important variability occurs. Seasonally, ocean advection seems to play an important role for SST variations of the eastern Pacific warm pool. Interannual variability in the eastern Pacific warm pool may be largely due to a direct oceanic connection with the ENSO variability at the equator. Variations in temperature, stratification, insolation, and productivity associated with ENSO have implications for phytoplankton productivity and for fish, birds, and other organisms in the region. Long-term changes in ENSO variability may be occurring and are briefly discussed. This paper is part of a comprehensive review of the oceanography of the eastern tropical Pacific.     

海表水温变动对东、黄海鲐鱼栖息地分布的影响

海表水温(SST)通常是表征鱼类栖息地分布的主要指标。本文根据1999—2007年我国大型灯光围网的鲐鱼生产统计数据,结合海洋遥感获得的SST,分析了渔汛期间鲐鱼栖息地的适宜SST范围,探讨了SST变动情况下鲐鱼栖息地的变化趋势。研究结果表明,东、黄海鲐鱼7—12月的适宜SST范围为15~30℃。根据政府气候变化专门委员会(IPCC)第四份评估报告,本文拟定4种SST上升的情况,即(1)每月平均SST+0.5℃;(2)每月平均SST+1℃;(3)每月平均SST+2℃;(4)每月平均SST+4℃。结果显示,东、黄海鲐鱼的潜在栖息有明显向北移动的趋势,并且栖息地面积逐渐减小。研究认为,全球气候变化引起的SST上升,可能会对近海鲐鱼栖息地造成严重的影响。     

Distribution of suspended particulate matter in the northern Bohai Bay in summer and its relation with thermocline

Field observations of suspended particulate matter (SPM) in the Bohai Bay, China have not been widely reported. The aim of this paper is to describe the horizontal and vertical distribution of mass and volume concentrations of SPM, respectively, based on observed data at 312 stations in the northern Bohai Bay during summer of 2006. A numerical model ECOMSED coupled with a sediment transport module was also established to further discuss the mechanism of the thermocline effect on the vertical distribution of SPM. The mass concentrations of SPM exhibited high inshore values and low offshore values in the horizontal distribution; while in the vertical direction, characteristics of the volume concentration of SPM can be divided into two types: one with a sharp peak at depth of 10–15 m and another without. The peak value at the depth of the thermocline was resulted from concentrated phytoplankton. A numerical experiment further displayed that the thermocline can also prevent particles from being resuspended upward.     

Seasonal variation of suspended-sediment transport through the southern Bohai Strait

Based on field observations made in winter 2006 and summer 2007 and on multiscene MODerate resolution Imaging Spectrometer (MODIS) imagery, the seasonal variation of suspended-sediment transport in the southern Bohai Strait and its possible mechanisms are examined. The field observations in two different seasons allow an exponential empirical model to be used to retrieve suspended-sediment concentration (SSC) from MODIS imagery. Both the field-survey data and the MODIS-derived SSC show that the sediment transport in the southern Bohai Strait has a significant seasonal variation due to the seasonally varying thermohaline structure of the water column and the hydrodynamics resulting from the seasonally alternating monsoons. The SSC in winter is approximately 3–10 times higher than in summer. Considering the seasonal variation of water flux (WF) and SSC, the annual sediment flux (SSF) through the southern Bohai Strait is estimated to be approximately 40.0 Mt yr⁻¹, about 4–8 times previous estimates, which did not take into account seasonal variation. Although the Huanghe (Yellow River) discharges a large amount of sediment in the summer, the SSF through the southern Bohai Strait in the winter (∼32.0 Mt) is about 4 times greater than it is in the summer. The strong seasonal variability of SSF through the southern Bohai Strait indicates that strong resuspension along the coast of the Huanghe delta in winter and enhanced longshore transport by coastal currents due to winter monsoon activity might be the major mechanisms of cross-strait transport of sediment in winter.     

基于碳、氮稳定同位素的雅浦海沟底栖生物食物来源和营养级初探

为了分析雅浦海沟中底栖生物群落的食物来源和营养级,本研究分析了雅浦海沟真光层中浮游植物和浮游动物、海底沉积物和巨型底栖生物（海绵、海参、海蛇尾、海星、海葵和钩虾）中的碳、氮稳定同位素组成。研究发现雅浦海沟真光层中的浮游植物和浮游动物δ13C值[（-22.8±0.4）‰和（-21.8±0.8）‰]和δ15N值[（5.4±0.4）‰和（6.8±0.2）‰]与巨型底栖生物的δ13C值（-20.1‰~-16.8‰）和δ15N值（11.9‰~17.9‰）的差异超过了一个营养级,表明作为底栖生物的初始食物来源的浮游植物和浮游动物在向下输送的过程中经历了食物链传递和细菌的降解。巨型底栖生物的δ15N和δ13C值之间无显著的相关性,此外不同物种之间营养级也存在明显差异,表现为海绵的营养级相对较高（3.4~4.7）,海参（3.3~3.6）、海蛇尾（3.4~3.5）和海星（3.2~3.7）的营养级较为接近,钩虾（2.9~3.3）和海葵（3.1）的营养级则相对略低,反映了底栖生物不同物种之间食物来源的多样化。     

底栖有孔虫(Florilus decors)壳体微量元素/钙比值的LA-ICP-MS原位微区分析方法及应用——以长江口外海域为例

深海沉积物中有孔虫壳体的微量元素、同位素测试技术已较为成熟,而河口近海沉积物中有孔虫壳体元素微区测试却鲜有报道。本文报道了一种利用激光剥蚀电感耦合等离子体质谱（LA-ICP-MS）分析法测试取自长江口外沉积物中的有孔虫壳体元素/钙比值的技术,研究了氦气载气流量、能量密度、束斑大小、激光剥蚀频率等参数对测试结果的影响,优化了活体有孔虫原位分析方法。应用确定的测试条件对2016年7月取自长江口外的底栖有孔虫优美花朵虫样品（Florilus decors）进行测试,发现Mg、Sr等元素/钙比值在壳体不同位置上无显著差异,而Mn的数据较为离散,可能与有孔虫生长过程中经历的水环境及其变化有关。