1961—1987年与1988—2014年辽宁省气候区划界线变化及可能气候成因

基于同一区划方法、指标体系,使用1961—2014年辽宁省52站气象观测资料,分析辽宁省气温、气候区划指标、范围及界线的变动特征。结果表明：辽宁省年均气温在1988年发生一次突变,突变后气温开始显著上升;≥10 ℃积温日数比较显著地响应气温突变,而干燥指数、7月平均气温变化不显著。在空间分布上区划指标值均存在不同程度的变化。① 全省≥10 ℃积温日数均出现增加,但在中西部地区显著增加;② 在盘锦-抚顺一线以北（南）,气候总体呈不显著变湿（干）趋势;③ 7月平均气温呈缓慢上升趋势。区划范围及界线位置出现更加显著地变化：① 暖温带范围主要向北向东扩展,中温带向东收缩;② 半湿润区范围主要向北向西扩展,半干旱区向西北方向收缩,湿润区范围基本不变;③ Tb范围显著向北向东扩展,Ta范围向北向东收缩。在此基础上分析了气候格局变化的可能气候成因,发现突变后≥10 ℃积温日数期间500 hPa高度场增加与4月和10月东亚冬季风减弱,4—10月东北冷涡持续天数增加和7月500 hPa高度场增加,可能分别是温度带,Tb区、Ta区和半湿润区、半干旱区变化的原因。     

Effect of carbon and nitrogen ratio control on Artemia growth,water quality,biofloc microbial diversity under high salinity and zero-water exchange culture condition

Biofloc technology has been applied successfully in the intensive aquaculture of several fish and shrimp species.The growth of heterotrophic microorganisms can be stimulated through adding extra carbon,which reduces the nitrogen level in the water and provides microbial protein to the animals.However,most of the studies and practical applications have been conducted in freshwater and marine environment.This paper focused on brine shrimp Artemia that lives in high salinity environment together with other halophilic or halotolerant microorganisms.The effect of carbon supplementation on Artemia growth,water quality,and microbial diversity of bioflocs was studied in the closed culture condition without any water exchange.The salinity of the culture medium was 100.A 24-d culture trial was conducted through supplementing sucrose at carbon/nitrogen(C/N)ratio of 5,15,and 30(Su5,Sul5,and Su30),respectively.The culture without adding sucrose was used as a control.Artemia was fed formulated feed at a feeding ration of 60% recommended feeding level.The results showed that sucrose supplementation at higher C/N ratio(15 and 30)significantly improved the Artemia survival,growth and water quality(P0.05).Addition of sucrose at C/N ratio of 15 and 30 significantly increased biofloc volume(BFV)(P0.05).The Illumina MiSeq sequencing analysis showed that supplementing carbon at C/N ratio of 15 had a better total bacterial diversity and richness,and shaped the microbial composition at genera level.This study should provide information for studying the mechanism of biofloc technology and its application in high salinity culture conditions.     

Impacts of Coastal Reclamation on Natural Wetlands in Large River Deltas in China

Little information is available on the impacts of coastal reclamation on wetland loss in large-river deltas at a regional scale.Using remote sensing data of coastal wetland and reclamation in four deltas in China from 1978 to 2014, we tracked their continuous area changes in four periods: 1978–1990, 1990–2000, 2000–2008, and 2008–2014. The areal relation between wetland loss and reclamation was quantified and used to identify coastal reclamation mode intensity coupled with another three indicators: reclamation rate,accretion rate and land-use intensity of coastal reclamation. The results showed that coastal reclamation driven by economic development reduced, or even reverse the original growth of delta which was determined by the offset between wetland acceleration rate and wetland loss rate. Generally, the area of reclamation showed a positive linear correlation with the area of wetland loss. The findings imply that human activities should control reclamation rate and intensity to alleviate total wetland loss and maintain wetland ’net gain’.Inappropriate coastal reclamation modes can magnify total wetland loss;therefore, coastal reclamation with a slow increment rate and low impervious surface percent is of great importance for sustainable development in future coastal management.     

条斑紫菜酵母双杂交文库的构建及PyMAPK5互作蛋白的筛选

为进一步研究条斑紫菜促分裂原活化激酶家族PyMAPK5的下游互作蛋白,理解其生物学功能,本研究通过酵母双杂交的方法进行其相互作用蛋白的筛选。提取不同温度和失水逆境胁迫下的RNA,利用Invitrogen体系构建条斑紫菜酵母双杂交cDNA文库,其库容为1.44×107CFU,重组率为91.8%。以pGBKT7-PyMAPK5为诱饵蛋白载体,利用共转化方法,从文库中筛选得到26个与PyMAPK5互作的候选蛋白。候选蛋白集中在光系统II相关蛋白、捕光蛋白、微管蛋白、ATP酶、GTP结合蛋白及假设蛋白等。微管蛋白、捕光蛋白、光系统II蛋白一对一验证结果为阳性,表明在酵母体内存在互作。本研究为阐明条斑紫菜PyMAPK5与其互作蛋白的关系及解析PyMAPK5下游作用机制奠定了基础。     

养殖裙带菜机械损伤防御应答的初步研究

以裙带菜(Undariapinnatifida(Harv.)Suringar)幼孢子体为材料,测定藻体对机械损伤的防御应答。结果显示:与对照组相比,机械损伤会诱导藻体内活性氧含量上升,以及抗氧化酶活性和抗氧化物含量的变化。当裙带菜受到机械损伤后,体内O2-和H2O2以及MDA等物质迅速积累,藻体的总抗氧化能力上升、CAT和POD活性在短时间内迅速升高、GSH和ASA迅速合成,从而在较短时间内控制活性氧以及MDA含量;与对照组相比,SOD活性仅在3h内高于对照组。该实验结果表明裙带菜幼孢子遭受机械损伤后的抗氧化系统会参与调节机体活性氧平衡,这种抗氧化机制的存在可以更快速、更节能、更有效地修复藻体所受损伤。     

深圳市浴场用海定级研究

海域分等定级是制定海域使用金征收标准和开展海域资产价格评估的前提和依据。借鉴《海域定级技术指引(试行)》,遵循资源禀赋差异、收益差异、节约利用和生态用海的原则选取海域自然条件和海域区位条件2个定级因素及海水质量、海洋自然灾害等10个评价因子,构建深圳市浴场用海定级的评价指标体系。采用综合指数评价法计算出207个评价单元的得分情况,通过聚类分析方法和变异系数(CV)来校核级别,最终将浴场用海的海域划分为Ⅰ级(22.22%)、Ⅱ级(33.82%)和Ⅲ级(43.96%)3个级别。研究成果为海洋管理部门制定浴场用海不同级别的基准价格提供了依据。     

航空重力垂直梯度探测潜艇方法研究

航空重力梯度测量属于被动探测,抗干扰能力强,如果能和其他探潜手段相配合将极大地提高航空搜潜的效率。针对航空重力梯度测量是否能够用于探测潜艇的问题,依据俄亥俄级弹道导弹核潜艇的结构特点,建立了适用于重力梯度计算的潜艇模型,分别给出了潜艇外壳、内部质量亏损产生的重力垂直梯度的计算方法,并对不同精度重力梯度仪可探测的潜艇重力垂直梯度值进行了计算,从航空反潜的角度对探测潜艇效果进行了分析。经计算表明,如航空重力梯度仪精度达到10~(-2) E,将具备一定的实际探潜效能;如精度达到10~(-4) E,反潜机搜索宽度可与现有航空磁性探潜相当。     

海底玄武岩中次生组分HCl溶解实验研究

次生组分沉淀是海底岩石中普遍存在的现象,次生组分去除是减少次生组分干扰、获取海底岩石高质量数据的关键。选择西太平洋麦哲伦海山区MK海山相对新鲜的玄武岩作为研究对象,经研磨、均一化后将粉末样品分为49份,其中48份分别以0.25,0.50,1.00,1.50,2.00,2.50 mol/L六种浓度的足量HCl溶解0.5,1,2,4,8,16,32和64 h后,选取溶解残渣测定主要氧化物和特征微量元素质量分数,确定HCl溶解法在去除次生组分的同时不会对海底新鲜玄武岩中原生组分造成影响的HCl浓度-时间组合。结果表明,在HCl浓度为1.00～2.50 mol/L,溶解时间为0.5～2 h的12种HCl浓度-时间组合均可保证海底玄武岩中原生组分的安全,并可有效去除次生组分。综合海底蚀变玄武岩次生组分去除实验研究,推荐使用1.00～2.00 mol/L HCl,溶解时间在0.5～1 h的实验方案。     

Primary molecular basis of androgenic gland endocrine sex regulation revealed by transcriptome analysis in Eriocheir sinensis

In crustaceans, the male sexual dif ferentiation and maintenance are specially regulated by androgenic gland(AG). However, little is known about the genes involved in the regulation process.RNA-Seq was performed on AG with ejaculatory duct(AG_ED) and ejaculatory duct(ED) as control in Eriocheir sinensis, one of the most important economic and ?shery crabs with typically sex dimorphism. A total of 925 unigenes were identi?ed as dif ferentially expressed genes(DEGs) and the expression of nine genes randomly selected was con?rmed by qRT-PCR. 667 unigenes were up-regulated in AG_ED, being supposed to be AG preferential genes. Among them, the full length of i nsulin-like androgenic gland factor( IAG) cDNA named as Es-IAG was obtained as a logo gene of AG, which together with the genes i nsulin-like receptor( INR), and s ingle insulin binding domain protein( SIBD), might constitute the sex regulation pathway. Several sex related genes were identi?ed, and their function will have to be investigated. Also,the identi?cation of j uvenile hormone epoxide hydrolase 1( JHEH1), ecdysteroid 22-hydroxylase( DIB) and e cdysone receptor( ECR) preliminarily clari?ed the molecular regulation mechanism of eyestalk-AG-testis axis, which plays important roles in molting and reproduction. The results will enhance our understanding for the molecular basis of the AG involved in male sex regulation in crabs.