T17断块岩性地层油气藏高效勘探开发模式研究

断陷湖盆缓坡带是油气运移的优势指向区,具备形成岩性地层油气藏的优越条件.本文采用地球物理技术与石油地质分析相结合的方法,分析了T17断块岩性地层油气藏的地质特征与油气展布规律,认为：（1）油气成藏受不整合面、岩性及构造因素综合控制;(2)单个含油砂体的油层在上倾部位受储层超覆尖灭线控制,下倾方向受油水界面控制;(3)多个含油砂体叠和构成了岩性地层油气聚集区带,整个区带油气层纵向上呈阶梯状产出,平面上由构造低部位向构造高部位呈条带状展布.据此,总结该区油气藏的高效勘探开发模式,并应用到该断块的勘探开发实践中,取得了较好的效果.     

Principal aspects of the Ordovician biotic radiation

The Ordovician radiation is among the major bioevents in earth history that have drawn great attention from geologists all over the world, and there have been hundreds of publications treating various aspects of this radiation. The study of the event involves a broad spectrum of research fields and concerns many scientific questions. Many opinions and hypotheses have been proposed on the triggering and controlling factors of the radiation. Herein the authors critically review the study of the Ordovician rad...     

极干旱区稀疏荒漠植被地上生物量遥感估算

以库姆塔格沙漠南部阿尔金山北麓山前戈壁区为研究区,借助无人机影像数据准确提取植被覆盖区;采用高空间分辨率WorldView-3数据的估算结果对中空间分辨率Landsat-OLI数据的估算结果进行修正;选取红光反射率波段(RED)、近红外反射率波段(NIR)、比值植被指数(RVI)、归一化植被指数(NDVI)和修正土壤调...     

Analysis of phospholipids in microalga Nitzschia closterium by UPLC-Q-TOF-MS

Precise structural identification of phospholipids in the microalga Nitzschia closterium has been established using ultra performance liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry (UPLC-ESI-Q-TOF-MS) for direct analysis of total lipid extracts. Mass spectrometry was performed in reflective time-of-flight using electron spraying ionization in negative mode. Phospholipid molecular species identification was based on the characteristic product ions and neutral loss yielded by different phospholipids under ESI-MS/MS mode. The molecular species were confirmed by the carboxylate anions produced by phospholipids in negative mode; the regiospecificity of the two acyl chains was determined from the ratio of sn-1 to sn-2 carboxylate anion abundances. As a result, 18 lipid molecular species were identified for the first time in this microalga, comprising seven phosphatidylcholines (PC), two phosphatidylethanolamines (PE), two phosphatidylinositols (PI), and seven phosphatidylglycerols (PG). Lipid standards of PC, PE, PI, and PG were added to the total lipids as internal standards for semiquantitative analysis, revealing concentrations of phospholipids in this species between 0.09 and 3.37 nmol/mg. This method can produce a full structural profile of intact phospholipid molecular species and can be used for study of the physiological and ecological functions of lipids by monitoring their individual changes over time.     

Rapid, sensitive detection of Vibrio anguillarum using loop-mediated isothermal amplification

Vibrio anguillarum is an important bacterial pathogen of aquatic organisms and a significant problem in aquatic farming. The rapid detection and identification of V. anguillarum, and other pathogens that infect marine organisms, is crucial to effective disease management. In this study, we developed a loop-mediated amplification (LAMP) assay to detect V. anguillarum in an hour in a single tube without the need for thermal cycling. Conserved regions of the metalloproteinase (empA) gene of V. anguillarum served as the targets for primer design. A fragment of the empA gene was amplified at 65°C in the presence of the primer mixture and Bst DNA polymerase. In the optimized LAMP assay, 6.7 pg of V. anguillarum DNA could be detected. Six strains of V. anguillarum and 17 strains of non-V. anguillarum bacteria were used in this study to evaluate the species specificity of the primers. The six V. anguillarum strains gave a positive result in the LAMP assay. This method was also validated in V. anguillarum-infected fish. This LAMP method is more sensitive than PCR in the detection of V. anguillarum and shows good species specificity. The LAMP assay is therefore an effective method for the quick detection of V. anguillarum both in the laboratory and in the field.     

Remote sensing of ecosystem services: An opportunity for spatially explicit assessment

Ecosystem service is an emerging concept that grows to be a hot research area in ecology. Spatially explicit ecosystem service values are important for ecosystem service management. However, it is difficult to quantify ecosystem services. Remote sensing provides images covering Earth surface, which by nature are spatially explicit. Thus, remote sensing can be useful for quantitative assessment of ecosystem services. This paper reviews spatially explicit ecosystem service studies conducted in ecology and remote sensing in order to find out how remote sensing can be used for ecosystem service assessment. Several important areas considered include land cover, biodiversity, and carbon, water and soil related ecosystem services. We found that remote sensing can be used for ecosystem service assessment in three different ways: direct monitoring, indirect monitoring, and combined use with ecosystem models. Some plant and water related ecosystem services can be directly monitored by remote sensing. Most commonly, remote sensing can provide surrogate information on plant and soil characteristics in an ecosystem. For ecosystem process related ecosystem services, remote sensing can help measure spatially explicit parameters. We conclude that acquiring good in-situ measurements and selecting appropriate remote sensor data in terms of resolution are critical for accurate assessment of ecosystem services.     

天津滨海新区应用地源热泵系统可行性分析及建议

介绍了滨海新区第四系水文地质条件、地温场等特征,结合地下水地源热泵系统和垂直地埋管地源热泵系统的特点及相关政策,对两种类型的地源热泵系统在滨海新区应用的可行性进行了分析。得出存在的主要问题包括咸淡水串层、回灌、地面沉降及热平衡等,建议在应用地源热泵系统时要合理设计,确保咸淡水不串层,并进行相应的原位热响应试验,研究热平衡和回灌问题,尽快开展该区浅层地热能的调查评价和开发利用专项规划工作。     

有限元与改进单纯形法联合编程技术在位移反分析中的应用

根据弹性地基梁法基本思想,考虑基坑分步开挖和逐级加撑的动态施工因素,通过有限元计算模拟基坑实际开挖过程。结合现场监测数据,采用位移反分析方法反演各土层m 值,并准确预测后续工况支护体的变形。采用有限元通用软件ANSYS内嵌的APDL语言编程建立支护体有限元计算模型,并应用数学软件MATLAB编制“改进单纯形法”优化程序,编写了接口程序,实现了MATLAB对ANSYS模型的实时循环调用和逐步寻优。武汉长江隧道武昌明挖段基坑工程算例证明,基于单纯形法的联合编程技术能方便、高效地被应用于支护体位移反分析预测中。建立了基于本工况预测下一工况或相邻断面的扩展型位移反分析预测模型,预测结果与实测结果拟合程度较高。     

强夯激励下黄土边坡动力响应模型试验研究

设计并完成了1:20比尺的黄土边坡大型动力模型试验,探讨了模型试验中的相似律、边界条件处理等问题;研究了强夯激励下黄土边坡的动力特性变化规律与动力响应规律,以及边坡坡率对动力特性和动力响应的影响。试验结果表明,边坡坡率越大,动力响应幅值越大;强夯激励下黄土边坡的动力响应幅值均随冲击荷载作用而产生并迅速衰减;振动周期在1 s之内,主频率在25～45 Hz之间,不会出现振动叠加现象。强夯激励下黄土边坡对径向加速度的放大效应更显著,下部边坡以竖向振动为主,上部边坡以径向振动为主。黄土边坡的边缘部位对强夯冲击振动的反应幅值较之内部存在放大现象。沿坡高方向,边坡对输入加速度具有明显的放大作用,坡顶处的放大效应最为显著。试验结果有助于揭示黄土边坡在强夯激励下的动力响应规律,为黄土地区的工程设计和施工提供有益的参考     

隧道围岩结构地震动稳定性分析的动力有限元强度折减法

为了得到隧道围岩结构的地震动安全系数,借助通用有限元软件ANSYS,首先对水平地震作用下的模型进行模态分析,得到质量阻尼系数和刚度阻尼系数;其次由静力分析模型得到竖向边界上的水平向支座反力,然后将结构自重转化为温度边界条件,通过热分析得到模型各节点的温度,从而实现在动力分析中考虑重力的影响;最后采用悬臂梁动力分析模型,导入热分析获得的模型各节点的温度,并在竖向边界上施加水平向支座反力,通过不断折减围岩塑性区的凝聚力c和内摩擦角?,直到计算不收敛为止,从而得到隧道围岩结构的地震动安全系数。数值算例结果表明：采用的方法是可行的,将围岩结构自重转化为节点温度的措施解决了以往动力分析不能考虑结构自重的难点,进而为以后地震作用下隧道动力安全系数的计算及其工程应用提供了理论依据。