The multiple chronological techniques applied to the Lake Suigetsu SG06 sediment core,central Japan

The varved sediment of Lake Suigetsu (central Japan) provides a valuable opportunity to obtain high‐resolution, multi‐proxy palaeoenvironmental data across the last glacial/interglacial cycle. In order to maximize the potential of this archive, a well‐constrained chronology is required. This paper outlines the multiple geochronological techniques being applied – namely varve counting, radiocarbon dating, tephrochronology (including argon–argon dating) and optically stimulated luminescence (OSL) – and the approaches by which these techniques are being integrated to form a single, coherent, robust chronology. Importantly, we also describe here the linkage of the floating Lake Suigetsu (SG06) varve chronology and the absolute (IntCal09 tree‐ring) time scale, as derived using radiocarbon data from the uppermost (non‐varved) portion of the core. This tie‐point, defined as a distinct (flood) marker horizon in SG06 (event layer B‐07–08 at 1397.4 cm composite depth), is thus derived to be 11 255 to 11 222 IntCal09 cal. years BP (68.2% probability range).     

Neotectonics of the Kharaulakh sector of the Laptev Shelf

Seismotectonic deformation and crustal stress pattern have been studied comprehensively in major seismogenic structures of the Kharaulakh sector of the Verkhoyansk fold system and adjacent parts of the Chersky seismotectonic zone. The study focuses on neotectonic structures, deep structure, and systems of active faults, as well as tectonic stress fields inferred by tectonophysical analysis of Late Cenozoic faults and folds. The results, along with geological and geophysical data, reveal main strain directions and structural patterns of crustal stress and strain in the Arctic segment of the Eurasia-North America plate boundary. The area is a junction of mid-ocean and continental structures evolving in a mixed setting of extension, compression, and their various combinations. The rotation pole of the two plates is presumably located near Buor-Khaya Bay. In this case, extension is expected to act currently upon the neotectonic structures north of the bay and compression to control those in the south and southeast. This inference is consistent with the identified zoning of stress and strain in the Kharaulakh sector.     

Theoretical and measured aeolian sand transport on a barrier island, Louisiana, USA

Over the past 100 years, the Isles Dernieres, a low lying barrier island chain along the coast of central Louisiana, Usa , has undergone more than 1 km of northward beach face retreat with the loss of 70% of its surface area. The erosion results from a long term relative sea level rise coupled with day to day wind and wave action that ultimately favours erosion over deposition. At a site in the central Isles Dernieres, 8 days of wind and beach profile measurements during the passage of one winter cold front documented aeolian erosion and deposition patterns under both onshore and offshore winds. For offshore winds, the theoretical erosion rate, based on wind shear velocity, closely matched the measured erosion rate; for onshore winds, the theoretical rate matched the measured rate only after being corrected by a factor that accounted for beach face morphology. In late February 1989, a strong cold front moved into coastal Louisiana. That cold front stalled over the Gulf of Mexico, resulting in 4 days of strong northerly winds at a study site on the Isles Dernieres. During those 4 days, the wind moved sand from the backshore to the upper beach face. When the cold front finally moved out of the area, the wind shifted to the south and decreased in strength. The onshore wind then restored some of the upper beach face sand to the backshore while increased wave activity moved the rest into the nearshore. The theoretical estimate of 1·28 m³ m^?1 for the rate of sand transport by the northerly wind compares well with the measured backshore erosion rate of 1·26 m³ m^?1, which was determined by comparing beach profiles from the start and end of the period of northerly winds. The theoretical estimate of 0·04 m³ m^?1 for the rate of sand transport by the southerly wind, however, is notably less than the measured rate of 0·45 m³ m^?1. The large discrepancy between the two rates can be explained by a difference in the shear velocity of the wind between the beach face, where the erosion occurred, and the backshore, where the wind stress was measured. Using an empirical relationship for the wind shear drag coefficient as a function of coastal environment, the theoretical estimate for the rate of sand transport by the southerly wind becomes 0·44 m³ m^?1     

The peterson limestone — early cretaceous lacustrine carbonate deposition in western wyoming and southeastern idaho

The lacustrine Peterson Limestone of western Wyoming and southeastern Idaho comprises six lithofacies throughout its 20,000 km² aerial extent. These are: (1) calcareous sandstone and shale, (2) red nodular limestone, (3) pink sandy micrite, (4) biomicrite, (5) graded silty micrite, and (6) limestone conglomerate. The first two represent floodplain deposition and paleosols, whereas the remaining are shallow nearshore and deeper lacustrine sediments.This sequence was developed in a large fresh, hardwater lake surrounded by fluvial systems and associated flood plains in a warm temperate climate. Well-oxidized sandy terrigenous rocks, together with calcareous paleosol nodules, indicate that flood-plain deposition both preceded and was concurrent with lacustrine carbonate deposition. Micrite and biomicrite formed in deeper parts of the basin while sandy and silty carbonate accumulated in shallower lake-margin areas. Less-calcareous shale units which are interbedded with deeper-water carbonate were deposited either during rapid basin subsidence and deepening of the lake center or during periods of slower carbonate precipitation. Turbidity currents and subaqueous debris flows generated along steeper lake margins, resulted in the deposition of rhythmic layers of graded silty micrite and diamictic limestone conglomerate in the deepest part of the basin. The carbonate-rich sediments comprising these two lithofacies were originally deposited on shallow lake-margin benches and subsequently were transported downslope toward the lake center.Comparison with other carbonate-precipitating lacustrine systems indicates that this lake was not like modern playas. Although no known modern lacustrine system is precisely like Lake Peterson, the flora, fauna, composition, and distribution of facies within modern temperate-region lakes most closely resemble those of the Peterson Limestone.     

Integration of high-resolution imagery and LiDAR data for object-based classification of urban area

This paper investigates the synergistic use of high-resolution multispectral imagery and Light Detection and Ranging (LiDAR) data for object-based classification of urban area. The main contribution of this paper is the development of a semi-automated object-based and rule-based classification method. In the implemented approach, the diverse knowledge about land use/land cover classes are transformed into a set of specialized rules. Further, this paper explores supervised Gaussian Mixture Models for classification, which have been primarily used for unsupervised classification. The work is carried out on test data from two different sites. Contribution of the LiDAR data resulted in a significant improvement of overall Kappa. Accuracy assessment carried out for aforementioned classification methods shows higher overall kappa for both the study sites.     

Effect of clayey media parameters on the negative response of a coincident loop1

The aim of this work is to consider the phenomenon of negative response and its detectability in a polarizable half-space formed of Clay-water mixture which commonly exists in water aquifers and which can be used as a simple indicator of the presence of Underground water. We make use of an analytical treatment for the transient voltage induced in a coincident loop lying on a half-space as a basis for our computations. A Cole-Cole model is used to represent the Clay with appropriate parameters. As saturated Clay is characterized by high conductivity and moderate chargeability, it is hardly detectable relative to the practical noise level. However, there are optimum Clay parameters that offer a maximum negative response. It is also observed that for a very short time constant τ in the Cole-Cole model, the amplitude of the negative response decreases with the decrease of the time constant due to the fast decay of the polarization current. Finally, it is found that there is an optimum value for the loop radius which gives the largest value of the negative response and this loop radius depends on the model parameters.     

Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of approximately 49 kDa size, and weak signals for proteins of approximately 41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of approximately 56 and 59 kDa size, and also a weak immunoreaction with a protein of approximately 43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14-40 ng/g DW in controls to 373-638 ng/g DW in exposed mussels.     

Measurement of DNA single-strand breaks in gill and hemolymph cells of mussels, Mytilus sp., collected on the French Atlantic Coast

DNA single-strand breaks were measured by the comet assay in both gill and hemolymph cells of mussels collected in 3 sampling areas of the French coast (Pointe du Castelli, Pen Bron and Saint-Nazaire Harbour). Whole mussel tissue samples were also collected for the chemical determination of PAH, PCB and heavy metal concentrations. In mussel, a higher level of DNA strand breaks was measured in gill than in hemolymph cells (p < 0.01). Despite a factor of contamination from 2 to 3 between sites, no difference in the extent of mussel DNA strand breaks was shown between sampling locations (p > 0.05), questioning the sensitivity of the assays used in biomonitoring studies.     

Cloning of a chub metallothionein cDNA and development of competitive RT-PCR of chub metallothionein mRNA as a potential biomarker of heavy metal exposure

Metallothionein has been assayed in a range of aquatic animal tissues as an indicator of metal exposure. We sequenced chub (Leuciscus cephalus) metallothionein cDNA which showed over 90% homology to common carp, goldfish and stone loach and 77% homology to rainbow trout sequences for metallothionein. We then used the extended primer method to develop an accurate quantitative competitive RT-PCR assay for metallothionein mRNA. RT-PCR was used to measure metallothionein mRNA in feral chub from a range of field sites, with different levels of heavy metal pollution, in the West Midlands, UK. Measurements were complemented by analysis of liver and gill metallothionein protein by capillary electrophoresis. There was no significant difference in the metallothionein protein levels between fish of different rivers and there was no evidence of elevation of mRNA at the sites of highest metal exposure. The level of metal exposure (e.g. zinc, nickel and cadmium each ranging between 15 and 28 microg/l ) at the pH (7.5-8.5) of these rivers appears insufficient to elevate hepatic or gill metallothionein in chub. A lack of elevation of hepatic metallothionein mRNA in chub exposed to zinc, copper and manganese for 24 h and 10 days in the laboratory also suggests a non-responsiveness of this species.