Ocean Science Journal - Brown tides caused by Aureococcus anophagefferens have occurred frequently in the coastal waters of Qinhuangdao since 2009. The Changli coast is the area most strongly... 相似文献
A model integrating geo-information and self-organizing map (SOM) for exploring the database of soil environmental surveys was established. The dataset of 5 heavy metals (As, Cd, Cr, Hg, and Pb) was built by the regular grid sampling in Hechi, Guangxi Zhuang Autonomous Region in southern China. Auxiliary datasets were collected throughout the study area to help interpret the potential causes of pollution. The main findings are as follows: (1) Soil samples of 5 elements exhibited strong variation and high skewness. High pollution risk existed in the case study area, especially Hg and Cd. (2) As and Pb had a similar topo-logical distribution pattern, meaning they behaved similarly in the soil environment. Cr had behaviours in soil different from those of the other 4 elements. (3) From the U-matrix of SOM networks, 3 levels of SEQ were identified, and 11 high risk areas of soil heavy metal-contaminated were found throughout the study area, which were basically near rivers, factories, and ore zones. (4) The variations of contamination index (CI) followed the trend of construction land (1.353) > forestland (1.267) > cropland (1.175) > grassland (1.056), which suggest that decision makers should focus more on the problem of soil pollution surrounding industrial and mining enterprises and farmland.
Rapid amplification of cDNA ends(RACE) and real-time polymerase chain reaction(RT-PCR) were carried out to analyze the CYP4 gene expression in polychaete Marphysa sanguinea exposed to benzo[a]pyrene(BaP) in this study. The full length of MsCYP4 cDNA was 2 470 bp, and it encoded 512 amino acids. The deduced amino acid sequence showed 47% identity with CYP4 F from frog Xenopus tropicalis and shared high homology with other known CYP4 sequences. To analyse the role of CYP4 in protecting M. sanguinea from BaP exposure, three BaP groups were established: 0.5, 5 and 50 μg/L. Polychaetes were sampled after 3, 7 and 12 d. At 0.5 μg/L, the effect of BaP on MsCYP4 gene expression increased with time prolonged. MsCYP4 gene expression curve showed Ushaped trend with time in 5 and 50 μg/L BaP groups. Therefore, MsCYP4 gene may play an important role in maintaining the balance of cellular metabolism and protecting M. sanguinea from BaP toxicity. 相似文献
Litter decomposition is the key process in nutrient recycling and energy flow. The present study examined the impacts of soil
fauna on decomposition rates and nutrient fluxes at three succession stages of wetland in the Sanjiang Plain, China using
different mesh litterbags. The results show that in each succession stage of wetland, soil fauna can obviously increase litter
decomposition rates. The average contribution of whole soil fauna to litter mass loss was 35.35%. The more complex the soil
fauna group, the more significant the role of soil fauna. The average loss of three types of litter in the 4mm mesh litterbags
was 0.3–4.1 times that in 0.058mm ones. The decomposition function of soil fauna to litter mass changed with the wetland succession.
The average contribution of soil fauna to litter loss firstly decreased from 34.96% (Carex lasiocapa) to 32.94% (Carex meyeriana), then increased to 38.16% (Calamagrostics angustifolia). The contributions of soil fauna to litter decomposition rates vary according to the litter substrata, soil fauna communities
and seasons. Significant effects were respectively found in August and July on C. angustifolia and C. lasiocapa, while in June and August on C. meyeriana. Total carbon (TC), total nitrogen (TN) and total phosphorus (TP) contents and the C/N and C/P ratios of decaying litter
can be influenced by soil fauna. At different wetland succession stages, the effects of soil fauna on nutrient elements also
differ greatly, which shows the significant difference of influencing element types and degrees. Soil fauna communities strongly
influenced the TC and TP concentrations of C. meyeriana litter, and TP content of C. lasiocapa. Our results indicate that soil fauna have important effects on litter decomposition and this influence will vary with the
wetland succession and seasonal variation.
Foundation item: Under the auspices of State Key Development Program for Basic Research of China (No. 2009CB421103), Key Program
of National Natural Science Foundation of China (No. 40830535/D0101), Knowledge Innovation Programs of Chinese Academy of
Sciences (No. KZCX2-YW-BR-16, KSCX2-YW-N-46-06) 相似文献
Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecular ecology, quantitative genetics and genomics. Therefore, it is extremely necessary to select several versatile, low-cost, efficient and time- and labor-saving methods to develop a large panel of microsatellite markers. In this study, we used Zhikong scallop (Chlamys farreri) as the target species to compare the efficiency of the five methods derived from three strategies for microsatellite marker development. The results showed that the strategy of constructing small insert genomic DNA library resulted in poor efficiency, while the microsatellite-enriched strategy highly improved the isolation efficiency. Although the mining public database strategy is time- and cost-saving, it is difficult to obtain a large number of microsatellite markers, mainly due to the limited sequence data of non-model species deposited in public databases. Based on the results in this study, we recommend two methods, microsatellite-enriched library construction method and FIASCO-colony hybridization method, for large-scale microsatellite marker development. Both methods were derived from the mi-crosatellite-enriched strategy. The experimental results obtained from Zhikong scallop also provide the reference for microsatellite marker development in other species with large genomes. 相似文献