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1.
Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding. 相似文献
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叶澜涛 《广东海洋大学学报》2006,26(2):92-96
池莉小说作为当代新写实小说的重要代表性文本引起了许多研究和评论。这些评论试图从以下四个方面来阐释池莉小说的各种意义:1、女性意识的觉醒;2、凡俗与无奈的生活图景;3、主题与结构的内外比较,4、综述及反思。通过对池莉小说近十年研究状况的梳理来加深对池莉小说的了解。 相似文献
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Microwave Type III bursts with positive frequency drifting rate were found by Stahli and Benz (1987) for first time. Type III events are especially interesting because they are well-known to be signatures of electron beams in coronal plasma, and they are effective means for diagnosting of source plasma. A microwave burst consisting of some microwave type III burst groups was registered at Beijing Astronomical Observatory with the 2545–2645–2840 MHz synchronous observing system. The distributions of frequency drifting rate, half power duration, and intensity for each impulse in the groups have been statistically analysed. From this analysis, some important parameters for the dynamic process in the flare are deduced and discussed. 相似文献
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采用PCR技术对2种亚洲龙鱼的mtDNAD_Loop全序列进行扩增和测序,序列结构分析和序列同源性比对结果表明,2种亚洲龙鱼的mtDNAD_Loop在靠近5’端有3个终止相关序列TAS(Ⅰ、Ⅱ、Ⅲ),靠近D_Loop的3’端有4个保守区域CSB1、CSB2、CSB3、CSB-D。在终止相关序列和保守区域之间是连续重复区域。经DNASP4.0软件分析,全序列中检测出多态位点数(S)为26,其中有17个转换,核苷酸多样性(Pi)为0.013,平均核苷酸差异数(K)为17.333。 相似文献
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应用地壳波浪与镶嵌构造学说对富氏谱分析法提取地壳垂直形变信息的科学性做了地质学意义上的阐释 ,并提出了根据多期形变资料提取特定波段上构造策应力的数学模型 相似文献
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基于可靠度理论的黄土高边坡优化设计 总被引:11,自引:2,他引:9
对黄土高边坡进行可靠性分析,应用数学原理和优化原理,建立了黄土高边坡的优化模型,对铜黄一级公路黄土高边坡进行分析验证。结果表明,该方法计算的结果与实际较接近.应用中易操作。 相似文献
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Qin Chengzhi 《中国地震研究》2004,18(2):178-187
The migration of strong earthquakes is an important research topic because the migration phenomena reflect partly the seismic mechanism and involve the prediction of tendency of seismic activity. Research on migration of strong earthquakes has mostly focused on finding the phenomena. Some attempts on getting regularity were comparatively subjective. This paper suggests that there should be indices of migration in earthquake dataset and the indexes should have statistical meaning if there is regularity in the migration of strong earthquakes. In this study, three derivative attributes of migration, i.e., migration orientation, migration distance and migration time interval, were statistically analyzed. Results in the North China region show that the migration of strong earthquakes has statistical meaning. There is a dominant migration orientation (W by S to E by N), a dominant distance (≤100km and on the confines of 300~700km), and a dominant time interval (≤1a and on the confines of 3~4a). The results also show that the migration will differ slightly with different magnitude range or earthquake activity phase. 相似文献
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本文作者利用IOS近似模型,计算了星际分子云条件下A型CH3CN-H2含超精细能级的碰撞跃迁速率系数。温度范围是20K-140K。为研究分子云与恒星形成区的物理、化学性质提供了有用的基础分子数据。 相似文献