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1.
Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding. 相似文献
2.
Vertical drains are usually installed in subsoil consisting of several layers. Due to the complex nature of the problem, over the past decades, the consolidation properties of multi‐layered ground with vertical drains have been analysed mainly by numerical methods. An analytical solution for consolidation of double‐layered ground with vertical drains under quasi‐equal strain condition is presented in this paper. The main steps for the computation procedure are listed. The convergence of the series solution is discussed. The comparisons between the results obtained by the present analytical method and the existing numerical solutions are described by figures. The orthogonal relation for the system of double‐layered ground with vertical drains is proven. Finally, some consolidation properties of double‐layered ground with vertical drains are analysed. Copyright © 2001 John Wiley & Sons, Ltd. 相似文献
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Microwave Type III bursts with positive frequency drifting rate were found by Stahli and Benz (1987) for first time. Type III events are especially interesting because they are well-known to be signatures of electron beams in coronal plasma, and they are effective means for diagnosting of source plasma. A microwave burst consisting of some microwave type III burst groups was registered at Beijing Astronomical Observatory with the 2545–2645–2840 MHz synchronous observing system. The distributions of frequency drifting rate, half power duration, and intensity for each impulse in the groups have been statistically analysed. From this analysis, some important parameters for the dynamic process in the flare are deduced and discussed. 相似文献
6.
DEVELOPMENTSTRATEGIESOFWATERANDLANDRESOURCESINTHEHEXIREGION,CHINA肖洪浪,高前兆,李福兴DEVELOPMENTSTRATEGIESOFWATERANDLANDRESOURCESINTHE... 相似文献
7.
应用地壳波浪与镶嵌构造学说对富氏谱分析法提取地壳垂直形变信息的科学性做了地质学意义上的阐释 ,并提出了根据多期形变资料提取特定波段上构造策应力的数学模型 相似文献
8.
张晓晖 《成都信息工程学院学报》2002,17(3):166-169
通过对影响能见度的因素的分析,提出了一种能见度预报方法-综合分析法,极大地提高了能见度的预报准确率。 相似文献
9.
本文报告一种用半导体激光器作光源,用子脉冲宽度100ns,长度为511的周期伪随机码对半导体激光器的输出作幅度调制;APD作光电检测器,高速数字信号处理器TMS320C25作信号处理的激光雷达组成方案和试作样机的实验结果。 相似文献
10.
In this paper the tidal phenomena on the Earth are concisely specified, including solid tides, ocean tides and atmospheric tides due to the luni-solar tide-generating force, and the Earth pole tide due to the motion of the Earth's rotation axis (polar motion); as well as their effects on the Earth rotation. The outcomes of scientific researches of Chinese astronomers on these topics are described in some detail. These researches deal with the mechanisms responsible for tidal effects on the earth rotation, and on the measurements of the Earth rotation parameters. Finally, the effects discovered by Chinese researchers on the measurements of the period and change in period of pulsars are discussed. These effects are very small in magnitude but not negligible. 相似文献