On determining membership of open clusters from relative proper motion

We discuss the determination of membership of 42 open clusters. Our analysis shows that Vasilevskis' mathematical model can be reasonably applied to this case. Our improved version of Sanders' method and our definition of cluster member based on the principles of discriminatory analysis effectively exclude stars of low probabilities. It is important in the study of open cluster to use only those with high probabilities. The effectiveness of the statistical method is closely related to the velocity distributions of the member and field stars. For fields where the error rate is high, it is better to combine other data than proper motion in determining membership.     

βCephei变星的脉动不稳定带的研究(英文)

在本文中我们对两种不同的 βCephei变星的理论脉动不稳定带作了比较。它们具有明显的不同之处。为了确定这种变星的脉动不稳定带的轮廓 ,我们根据 4 9颗 βCephei变星的观测资料作了统计研究。在从样本星的色指数 (B -V) ,(U -B)和视差得到它们的有效温度和光度之后 ,我们发现在赫罗图上它们大多数是位于主序带内的。而且这些样本星的质量都位于 7M⊙ 到 30M⊙ 之间。和理论模型的比较表明我们提出的具有光度上边界和红蓝边界的 βCephei变星的理论脉动不稳定带跟观测吻合得更好     

Current status of bacteriological parameters and DOC/POC in Xiamen coastal waters

The surface and bottom waters samples were collected from six locations in Xiamen western sea. The quantified estimation of bacterial production (3H-thymidine method) and observation of bacterial heterotrophic activity (14C-glucose method) have been made in order to have a better understanding of the role of marine bacteria and their activities. The results showed that the mean value of bacterial heterotrophic activity was 9×108 cells/(L.h) in the surface waters and 2.6×108 cells/(L.h) in the bottom waters. The mean value of bacterial production was 38×108 cells/(L.h) in the surface waters and 7.1×108 cells/(L.h) in the bottom waters. The relationship between bacterial production, heterotrophic activity, POC and DOC measured during this survey were discussed. The good understanding of the relationship between bacteria activity and total coliform was addressed.     

东太平洋地区DSDP573孔始新世末期的微玻璃陨石

通过研究 DSDP5 73孔的岩心样品 ,首次在该孔始新世与渐新世的界线地层含金属粘土层中发现了微玻璃陨石 ,且主要富集在其底部。一个样品 (5 g)中最多可达 12粒。这些微玻璃陨石主要呈球形、椭球形。用电子探针测试了其化学成分 ,Si O2 含量为 2 9.6 4%～ 5 8.93%,Al2 O3 为 3.42 %～ 2 0 .96 %,Fe O为 9.98%～ 2 9.5 5 %,Mg O为 4.32 %～ 16 .0 5 %,Ir为 0 .0 6 %～ 0 .81%。总体来说 ,微玻璃陨石的化学成分变化较大 ,可能与靶岩的成分变化较大有关。始新世末期北美微玻璃陨石场的展布方向约为 2 5 0°,这与太平洋板块运动方向改变所需要的矢量变化(2 45°)仅相差 5°,推测很可能是始新世末期陨星的撞击引起了太平洋板块在该时期的运动方向由 NNW突然变为NWW。     

An infinity of conservation laws of fKdV equation

An infinity of conservation laws of fKdV equation is derived in terms of the Miura and Gardner''s transform.The pseudo-mass and energy theorems are studied by the first two conservation laws.As a typical example,the theoretical mean wave resistance and the regional distribution of energy of the precursor soliton generation are determined by means of the first and the second conservation laws.     

麻痹性贝毒毒素研究Ⅰ.塔玛亚历山大藻的麻痹性贝毒毒素对运动神经末梢和全细胞钠离子通道的抑制作用研究

麻痹性贝毒(Paralytic Shellfish Poisoning，PSP)毒素由石房蛤毒素(saxitoxin，STX)及其衍生物组成，目前己发现20余种，在赤潮研究、分子生物学和神经生物学基础研究、医药、军事防化等方面都有应用潜力[其结构、类型和应用见本集刊王云峰等(2003)“麻痹性贝毒毒素的应用研究进展”一文]。由于PSP毒素的稀有来源和国际社会对STX交易的禁止，限制了国内PSP毒素应用及研究的全面深入展开，因此本文作者对PSP毒素进行了制备，并采用不同方法对制备的PSP毒素进行了研究。 PSP毒素能够选择性地可逆抑制可兴奋膜的电压依赖钠离子通道的开放，从而阻止神经冲动的发生和传导，使神经、肌肉丧失兴奋性(Frace et al.，1986；Penzotti et al.，1998)。本文利用神经束膜下记录和全细胞膜片钳技术，报道了从塔玛亚历山大藻(Alexandrium tamarense)中提取的PSP粗毒素对小鼠运动神经末梢膜电流和NG108-15细胞钠离子通道的作用研究结果，并与STX标准毒素的作用结果进行了比较。 NG108-15细胞是由小鼠神经母细胞瘤和大鼠胶质细胞瘤融合的杂交细胞，经分化剂分化后，显示诸+L1196如兴奋性、合成和释放乙酰胆碱、与培养肌细胞形成突触联系等多种神经细胞的基本特性(Hamprcht，1977)和Na+、K+、Ca2+等多种离子通道，已作为神经细胞模型被广泛应用于分析药物对离子通道作用的研究(Enomoto et al.，1992； Docherty et al.，1992；Shi et al.，1993；Hu et al.，1997a；Hu et al.，1997b)；发育出具有Na+内流支持的锋电位(Hamprecht,1977)，该电位是分析作用于膜钠离子通道药物的好材料。     

珠江口伶仃洋温度分布特征

依据实测资料，分析了伶汀洋冬、夏季的水温分布特征及其变化。冬季湾内水温的分布和变化较单一，夏季由于河流入海流量增大，河流与海洋水体的相互作用加强，明显可见两个冷、暖水锋在河口湾中段相交，上段为高温的河流淡水控制区，下段为低温的陆架水控制区，两者显著的特点是其控制区内温度变化很小，而在锋面转折地带的河口湾中段，盐淡水混合强烈，其温度变化大，温度等值线呈倾斜分布。     

Long-term changes in the fish community structure from the Tsushima warm current region of the Japan/East Sea with an emphasis on the impacts of fishing and climate regime shift over the last four decades

Japanese fisheries production in the Japan/East Sea between 1958 and 2003 increased to their peak (1.76 million tons) in the late 1980s and decreased abruptly with the collapse of Japanese sardine. Catch results for 58 fisheries and various environmental time-series data sets and community indices, including mean trophic level (MTL) and Simpson’s diversity index (DI), were used to investigate the impacts of fishing and climate changes on the structure of the fish community in the Tsushima warm current (TWC) region of the Japan/East Sea. The long-term trend in fisheries production was largely dependent on the Japanese sardine that, as a single species, contributed up to 60% of the total production in the Japanese waters of the Japan/East Sea during the late 1980s. Excluding Japanese sardine, production of the small pelagic species was higher during 1960s and 1990s but lower during 1970s and 1980s. This variation pattern generally corresponds with the trend in water temperature, warmer before early 1960s and after 1990s but colder during 1970s and 1980s. The warm-water, large predatory fishes and cold water demersal species show opposite responses to the water temperature in the TWC region, indicating the significant impact of oceanic conditions on fisheries production of the Japan/East Sea. Declines in demersal fishes and invertebrates during 1970s and 1980s suggested some impact of fishing. MTL and DI show a similar variation pattern: higher during 1960s and 1990s but lower during 1970s and 1980s. In particular, the sharp decline during the 1980s resulted from the abundant sardine catches, suggesting that dominant species have a large effect on the structure of the fish community in the Japan/East Sea. Principal component analysis for 58 time-series data sets of fisheries catches suggested that the fish community varied on inter-annual to inter-decadal scales; the abrupt changes that occurred in the mid-1970s and late 1980s seemed to correspond closely with the climatic regime shifts in the North Pacific. These results strongly suggest that the structure of the fish community in the Japan/East Sea was largely affected by climatic and oceanic regime shifts rather than by fishing. There is no evidence showing “fishing down food webs” in the Japan/East Sea. However, in addition to the impacts of abrupt shifts that occurred in the late 1980s, the large predatory and demersal fishes seem to be facing stronger fishing pressure with the collapse of the Japanese sardine.     

中国沿海亚历山大藻(Alexandrium)核糖体rDNA部分序列分析及该藻属分子系统进化研究

分析了几株自南海及东海分离的亚历山大藻的rDNA部分序列信息,其中包括核糖体大亚基(LSU)rDNA的5′端D1-D2区序列,以及5.8SrDNA和ITS区序列;同时也对实验室保种的部分来自其它国家和地区的亚历山大藻相关序列进行了测序和分析,并以此作为序列分析中的参考。采用ClustalX及MEGA2软件对所得到的序列信息进行了综合分析与对比。结果表明,分离自南海的塔玛亚历山大藻(Alexandriumtamarense)和分离自东海的链状亚历山大藻(A.catenella),即便是在ITS区和LSUrDNA等高变区,其序列信息也完全一致。与基因库中搜索到的其它亚历山大藻rDNA序列信息相比较,中国沿海的塔玛/链状亚历山大藻序列更接近于塔玛复合种的“亚洲温带”基因型。对于分离自南海的另外两株未定种的亚历山大藻,通过对比序列信息,发现它们与相关亚历山大藻(A.affine)非常接近。分离于我国台湾地区的微小亚历山大藻(A.minutum)在序列上与分离自新西兰的藻株相似,而与分离自欧洲的微小亚历山大藻藻株相差较大。中国沿海亚历山大藻rDNA序列信息的获得为针对有毒藻种设计特异性核酸探针,发展灵敏快速的生物检测技术奠定了基础。     

棉粕蛋白酶解物对异育银鲫(Carassius auratus gibelio)消化、生长和胰蛋白酶mRNA表达量的影响

以棉粕蛋白为酶解底物,用枯草杆菌蛋白酶对其进行酶解,以酶解产物1.5%和3.0%两个梯度等量替代鱼饲料配方中棉粕,在室内流水养殖系统中喂养异育银鲫鱼种[体重为(30±2)g]65天。测定鱼的生长、营养物质表观消化率、消化蛋白酶活性及肝胰脏中胰蛋白酶mRNA表达水平等指标。结果表明,添加1.5%和3.0%棉粕酶解产物的鱼在饲养35天后的特定增长率(SGR)分别比对照组高32.5%和56.7%,且差异显著(P<0.05);在饲养65天后,两组特定增长率分别比对照组高8.0%和21.0%,且差异极显著(P<0.01),肝胰脏中胰蛋白酶mRNA表达水平也随棉粕酶解产物添加梯度提高而相应提高,表明鱼的生长与消化蛋白酶mRNA表达水平相关。同时棉粕蛋白酶解物对肠道蛋白酶的活性和营养物质表观消化率都有促进作用,而棉粕蛋白酶解物对鱼肌肉成分并没有改变,这也表明棉粕蛋白酶解物在促进鱼生长、内源酶活性同时并未降低鱼的品质。