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1 INTRODUCTION Magnetotactic bacteria are able to respond and orient along the direction of terrestrial or artificial magnetic field, a behavior referred to as magneto- taxis. In the majority of magnetotactic bacteria, the magnetosomes are 25–120 nm in d… 相似文献
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通过纯培养和16SrRNA基因序列的相似性比对,并构建系统发育树对威海海域柄海鞘共附生细菌的多样性进行了研究。用2216E培养基从柄海鞘样品中分离到78株细菌,通过形态学特征和TCBS培养基选择性筛选后,选取30株具有代表性的菌株进行了16SrRNA基因测序和基于16SrRNA基因序列的系统发育多样性分析。结果表明,30株菌株分别属于细菌域的4个系统发育类群(Actinobacteria,Bacteroidetes,Firmicutes,Gro-teobacteria)的11个科,11个属。根据16SrRNA基因序列,大多数菌株与其系统发育关系最密切的模式菌株之间存在一定的遗传差异(16SrRNA基因序列相似性为91.82%~98.93%)。其中菌株HQW7,HQYD1,HQWD4,HQE1和HQE2与相关模式菌株的16SrRNA基因最高相似度仅分别为91.82%,92.51%,92.99%,93.52%,93.87%,这5株菌可能代表新的分类单元。威海海域柄海鞘共附生细菌存在着较为丰富的物种多样性和系统发育多样性,并可能存在新的物种。 相似文献
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Cultivable magnetotactic bacteria (MTB) in laboratory can provide sufficient samples for molecular microbiological and magnetic
studies. However, a cold-stored MTB strain, such as Magnetospirillum magneticum AMB-1, often loses its ability to synthesize magnetosomes and consequently fails to sense the external magnetic field. It
is therefore important to quickly recover vigorous bacteria cells that highly capable of magnetosome producing. In this study,
a modified capillary magnetic separation system was designed to recover a deteriorating strain of Magnetospirillum magneticum AMB-1 that long-term cold-stored in a refrigerator. The results show that all cells obtained after a 3-cycle treatment were
vigorous and had the ability to produce magnetosomes. Moreover, the 3rd-cycle recovered cells were able to form more magnetosome crystals. Compared with the colony formation method, this new method
is time-saving, easily operated, and more efficient for recovering vigorous MTB cells. 相似文献
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黑色葡萄状穗霉纤维素酶的纯化和性质 总被引:2,自引:0,他引:2
将筛选所得的黑色葡萄状穗霉 (Stachybotrys atra) S6 0 7发酵培养 96 h,发酵液离心去除菌体 ,上清液经超滤、Sephadex- G10 0柱层析、DEAE- Sepharose fast flow弱阴离子交换层析、CM-Sepharose fast flow阳离子交换层析、Q- Sepharose fast flow强阴离子交换层析及置换层析等步骤 ,得到了 4种电泳纯的酶组分 ,即内切纤维素酶 EG 、EG 、EG 和 β葡萄糖苷酶 (β Gase)。利用 SDS- PAGE测得 4种酶组分的分子量分别是 78.3、5 8.1、72 .4和 5 5 .6 k Da。 3种内切酶组分的最适温度都是 5 0℃ ,β-葡萄糖苷酶为 5 5℃ ,它们的最适 p H值分别是 6 .0、7.0、6 .5和 6 .0。底物专一性的实验表明 ,内切酶 、 、 都可作用于木聚糖。 相似文献
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根据调查设备搭载器的不同,将海底管道在位状态调查方法归为船载、自治水下机器人搭载(AUV 搭载)和无人遥控潜水器搭载(ROV搭载)三类;在浅水段采用船载调查,在深水段采用AUV搭载调查,在此基础上进行重点关注区域筛选,开展ROV调查,最后进行整体评价和分析对比,这种“(船载/AUV)+ROV”组合模式很好地实现了多种调查方法的优势互补。实际上,采用AUV搭载进行海底管道在位状态调查在国内业界尚属首次。调查所取得的管道位置、埋深、周边障碍物的分布以及管道人工处理情况等成果,为后期开展管道维护工作提供了重要的基础数据。此次成功实践可为以后从浅水到深水的管道在位状态调查提供经验和借鉴。 相似文献
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