Vertical distribution of the macrobenthic community and its relationships to trace metals and natural sediment characteristics in the lower Douro estuary, Portugal

Vertical distribution (0–15 cm) of the macrobenthic community and its relationships to natural sediment characteristics and trace metal contents and bioavailability were studied at five locations in the lower Douro estuary, Portugal. An analysis of vertical metal distribution, for the interpretation of anthropogenic impact on the estuarine sediments, was also investigated. Sediment characterisation included organic matter, grain size, metals (Al, Fe, Cu, Pb, Cr, Ni, Cd, Zn and Mn), acid volatile sulphide (AVS) and simultaneously extracted metals (SEM). The macrobenthic community had low diversity (14 species), was dominated by small size opportunists and seemed to be controlled mainly by natural factors such as grain size distribution, Al and Fe contents and sediment depth. The vertically heterogeneous distribution of macrobenthic community appears to affect redox status of the sediments and consequently metal bioavailability. Despite anthropogenic contamination in terms of Zn, Cu, Pb, Cr and Ni having already been detected in the north bank, the analysis of vertical distribution was essential for the identification of current anthropogenic contamination in terms of Zn, Pb and Cd in the south bank.     

Biomarkers of Exposure and Effects for Assessing Toxicological Risk of Endocrine Disrupters in Top Predators of the Mediterranean Sea

Abstract. Endocrine Disrupting Chemicals (EDCs) have the potential to alter hormone pathways that regulate reproductive processes in wildlife and fisheries. In this research the unexplored hypothesis that Mediterranean top predator species (such as large pelagic fish and marine mammals) are potentially at risk due to EDCs is investigated. In the Mediterranean environment, top predators accumulate high concentrations of polyhalogenated aromatic hydrocarbons (PHAHs) and toxic metals, incurring high toxicological risk. Here we illustrate the need to develop sensitive biomarkers for evaluation of toxicological risk in top marine predators (Xiphias gladius, Thunnus thynnus thynnus) and non-lethal techniques, such as non-destructive biomarkers, for the hazard assessment of threatened species exposed to EDCs, such as marine mammals ( Stenella coeruleoalba, Tursiops truncatus, Delphinus delphis and Balaenoptera physalus ).     

Multi-level assessment of chronic toxicity of estuarine sediments with the amphipod Gammarus locusta: II. Organism and population-level endpoints

This study aimed to test the performance of the amphipod Gammarus locusta (L.) in chronic sediment toxicity tests. It constitutes part of a multi-level assessment of chronic toxicity of estuarine sediments, integrating organism and population-level endpoints with biochemical markers responses. Here we account for organism and population-level effects, while biomarker responses were reported in a companion article. Five moderately contaminated sediments from Sado and Tagus estuaries were tested, comprising 3 muddy and 2 sandy sediments. These sediments either did not show acute toxicity or were diluted with control sediment as much as required to remove acute toxicity. Subsequent chronic tests consisted of 28-day exposures with survival, individual growth and reproductive traits as endpoints. Two of the muddy sediments induced higher growth rates in the amphipods, and improved reproductive traits. This was understood to be a consequence of the amount of organic matter in the sediment, which was nutritionally beneficial to the amphipods, while concurrently decreasing contaminant bioavailability. Biomarker responses did not reveal toxicant-induced stress in amphipods exposed to these sediments. One of the sandy sediments was acutely toxic at 50% dilution, but in contrast stimulated amphipod growth when diluted 75%. This was presumed to be an indication of a hormetic response. Finally the two remaining contaminated sediments showed pronounced chronic toxicity, affecting survival and reproduction. The sex ratio of survivors was highly biased towards females, and offspring production was severely impaired. The particulars of the responses of this amphipod were examined, as well as strengths versus limitations of the sediment test. This study illustrates the utility of this chronic test for toxicity assessment of contaminated estuarine sediments, with potential application all along Atlantic Europe.     

The tectonic regime in Italy inferred from borehole breakout data

In Italy, the horizontal stress directions are well constrained in many regions, but the tectonic regime is not well known because the stress magnitudes are unknown. Our intention is to improve the knowledge of crustal stress in Italy, both at shallow depth and in low seismicity areas. Therefore, we inferred the tectonic regime from the comparison between the depth of breakout occurrence and the physical properties of the rocks in 20 boreholes. The critical value of the maximum horizontal stress, for which the effective tangential stress at the borehole wall overcomes the rock strength to form breakouts, could be computed from rock strength and density. Comparing the theoretical stress distributions for different tectonic regimes with the depth distribution of breakout occurrence, it is possible to infer the tectonic regime that fits best to the breakout depth distribution. We investigated boreholes up to 6 km deep located in different tectonic environments over the Italian peninsula: the Po Plain, the Apenninic chain, the Adriatic foredeep and the Tyrrhenian Quaternary volcanic region. These wells are characterised by breakout data of good quality (A, B and C, according to World Stress Map quality ranking system). The results are in general agreement with the style of faulting derived from earthquake focal mechanisms and other stress indicators. Our results show a predominance of a normal faulting (NF) regime in the inner Apennines and both normal faulting and strike–slip faulting (SS) style in the surrounding regions, possibly also associated with changes in the tectonic regime with depth.     

Length-specific carbon content of theDaphnia population in a large subalpine lake, Lago Maggiore (Northern Italy): The importance of seasonality

We compared three methods for calculating the standing stock biomass ofDaphnia hyalina galeata population in Lago Maggiore during a single year. We applied body length/carbon regression equations (LCR) obtained under controlled laboratory conditions and directly from field data on carbon and body length to field data on length-specific abundance. Laboratory-derived equations satisfactorily described our field estimates of length-specific carbon content in spring. As the length-standardized carbon content decreased exponentially along the season, we included time of the year as an auxiliary variable, and tested whether it affected slope, intercept or both. The decrease had no effect on slope but reduced the intercept of the LCR. We also calculated biomass, using a single mean value from the literature. By comparing the results with those obtained by simple conversion of individuals number into carbon we might discriminate between the effect of changes in the size frequency distribution of the population and the decrease with time of the length-standardized carbon content. Although we tested the different models for non-ovigerous females, the results did not change when we included also data on females with eggs in the first three developmental stages, whereas they changed substantially when we included females with embryos. We formulate two hypotheses to explain this result and discuss possible implications for the calculation of the standing stock biomass ofDaphnia population.     

The organisation of fish assemblages in the regulated Lima basin, Northern Portugal

In order to understand the structure of fish assemblages in the modified Lima basin (Northern Portugal), two distinct datasets concerning the presence and abundance of fish species were subjected to multivariate analysis. On the River Lima two types of flow modification are present within kilometres of one another: (a) a reduced and constant flow due to hypolimnetic release; and (b) an intense and irregular flow. A comparison of their influence on fish assemblages revealed a gradient of assemblage types from tributaries to main river sites. The latter were characterised by a strong dominance of cyprinids, particularly Iberian barbel (Barbus bocagei). The former harboured two kinds of fish assemblages: those closer to the river mouth were dominated by the cyprinids Iberian chub (Squalius carolitertii) and Iberian nase (Chondrostoma polylepis), which were also frequently present in the main river; while in those further upstream the predominant species was the brown trout (Salmo trutta). Although explanatory variables such as distance from source, altitude, substrate coarseness and width were the primary correlates of fish assemblage composition, dam construction and flow regulation also had a significant effect upon assemblage structure, particularly by: i) reducing the importance of migratory species; ii) constraining the presence of trout in the regulated segments; and iii) simplifying the community, especially in the case of the constant and reduced flow regime.     

The study of metal contamination in urban topsoils of Mexico City using GIS

This research presents and discusses information concerning the spatial distribution of heavy metals (Pb, Cu, Zn, Ba, Co, Cr, Ni and V) in the urban environments of Mexico City using geographical information system and statistical analysis. Superficial soil samples (n = 146) were analyzed. The highest contamination indices were found in the north and center zone of the metropolitan area. In contrast, the surrounding rural fields show a lower impact grade. The higher concentrations of Pb, Cu, Zn and Ba were observed as being related to high vehicular traffic, nevertheless other elements such as Co, Cr, Ni and V do not show anthropogenic influence and their content can be attributed to the parental rock. The results are compared with previous surveys carried out in 2003 in order to evaluate temporal deposition trends. No changes were found on reported concentrations except for Cu and Zn, whose concentration has increased in later years. The results suggest that spatial distribution analysis and results in comparison with previous studies could be useful for the management and sustainable development of the metropolitan area of Mexico City.     

Understory and small trees contribute importantly to stemflow of a lower montane cloud forest

Stemflow (Sf) measurements in tropical rain and montane forests dominated by large trees rarely include the understory and small trees. In this study, contributions of lower (1‐ to 2‐m height) and upper (>2‐m height and <5‐cm diameter at breast height [DBH]) woody understory, small trees (5 < DBH < 10 cm), and canopy trees (>10‐cm DBH) to Sf per unit ground area (Sf_a) of a Mexican lower montane cloud forest were quantified for 32 days with rainfall (P) during the 2014 wet season. Rainfall, stemflow yield (Sf_y), vegetation height, density, and basal area were measured. Subsequently, stemflow funneling ratios (SFRs) were calculated, and three common methods to scale up Sf_y from individual trees to the stand level (tree‐Sf_y correlation, P‐Sf_y correlation, and mean‐Sf_y extrapolation) were used to calculate Sf_a. Understory woody plants, small trees, and upper canopy trees represented 96%, 2%, and 2%, respectively, of the total density. Upper canopy trees had the lowest SFRs (1.6 ± 0.5 Standard Error (SE) on average), although the lower understory had the highest (36.1 ± 6.4). Small trees and upper understory presented similar SFRs (22.9 ± 5.4 and 20.2 ± 3.9, respectively). Different Sf scaling methods generally yielded similar results. Overall Sf_a during the study period was 22.7 mm (4.5% of rainfall), to which the understory contributed 70.1% (15.9 mm), small trees 10.6% (2.4 mm), and upper canopy trees 19.3% (4.4 mm). Our results strongly suggest that for humid tropical forests with dense understory of woody plants and small trees, Sf of these groups should be measured to avoid an underestimation of overall Sf at the stand level.     

Exploring lava‐flow hazards at Pico Island,Azores Archipelago (Portugal)

Pico, the youngest island of the Azores Archipelago (Portugal), is characterized by a central volcano and a 30‐km‐long fissure zone. Its eruption rate is the highest of the Azores islands, with more than 35 eruptions in the last 2000 years. Here, we estimate the lava‐flow hazard for Pico Island by combining the vent opening probability derived from the spatial distribution of eruptive fissures, the classes of expected eruptions inferred from the physical and chemical characteristics of historical eruptions, and the lava‐flow paths simulated by the MAGFLOW model. The most likely area to host new eruptions is along a WNW–ESE trend centred on the central volcano, with the highest hazard affecting the two main residential zones of Lajes do Pico and Madalena. Our analysis is the first attempt to assess the lava‐flow hazard for Pico Island, and may have important implications for decision‐making in territorial management and future land‐use planning.