Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.     

建鲤基因组DNA的RAPD分析

用 1 5个随机引物对建鲤基因组DNA进行RAPD分析 ,结果显示 ,建鲤群体内的遗传相似系数为 0 91 81± 0 0 73 8,多态位点比例为 0 41 67,平均杂合度为 0 1 884。表明建鲤种质较纯 ,群体内的遗传变异程度较小。建鲤优良性状产生的分子学基础是等位基因的杂合程度较高     

观驾山—黑岛一带信息量法找金研究

本文以成矿地质模型为依据，对观驾山—黑山岛一带的化探、物探、遥感及地质等信息变量进行了详细的分析，选取了１０个信息变量２４个标志状态；在此基础上，运用信息量法对研究区内４５８个单元的信息量值进行计算，并由此确定了研究范围内的成矿远景区。事实表明，研究区信息量成矿预测是非常有效的     

Microwave Type III Burst Group in the 1993 October 2 Flare

Microwave Type III bursts with positive frequency drifting rate were found by Stahli and Benz (1987) for first time. Type III events are especially interesting because they are well-known to be signatures of electron beams in coronal plasma, and they are effective means for diagnosting of source plasma. A microwave burst consisting of some microwave type III burst groups was registered at Beijing Astronomical Observatory with the 2545–2645–2840 MHz synchronous observing system. The distributions of frequency drifting rate, half power duration, and intensity for each impulse in the groups have been statistically analysed. From this analysis, some important parameters for the dynamic process in the flare are deduced and discussed.     

毫秒级快速尖峰辐射事件的时间和频率特性

介绍了太阳22周峰年期间云南天文台米波射电频谱仪的观测结果。主要对1557个单个Spike的时间和频率分布作了统计分析，着重指出太阳快速精细结构的观测特征并定性地指出其产生机制。     

富氏谱分析法模拟地壳垂直运动的地学依据及其策应力模型

应用地壳波浪与镶嵌构造学说对富氏谱分析法提取地壳垂直形变信息的科学性做了地质学意义上的阐释 ,并提出了根据多期形变资料提取特定波段上构造策应力的数学模型     

滇西北和邻区均衡重力异常特征及其壳内构造

本文以爱黎-海斯卡宁均衡补偿假设模式为基础,利用圆模板编制了滇西北及邻区均衡重力异常图。分析了异常的基本特征与壳内构造的关系,并初步探讨了均衡重力异常和强震活动的相关性。     

激变变星0623＋71的光谱研究

本文提出了激变变星０６２３十７１的４个时间分辨光谱，覆盖轨道周期的７３％．其中的３个谱清楚地显示了各种光谱特征随轨道运动的变化，在流量、能谱以及谱线轮廓方面的这种变化正是对一个倾角不太大的激变变星所期望的．然而另一个曝光对分钟的谱，流量和视向速度与预期的相比都太大，很难加以解释，可能它是一次象ＡＥＡｑｒ中观测到的跃发事件．     

Protostellar angular momentum transport by spiral density waves

The gravitational collapse of molecular clouds or cloud cores is expected to lead to the formation of stars that begin their lives in a state of rapid rotation. It is known that, in at least some specific cases, rapidly rotating, slf-gravitating bodies are subject to instabilities that cause them to assume ellipsoidal shapes. In this paper we investigate the consequences of such instabilities on the angular momentum evolution of a star in the process of formation from a collapsing cloud, and surrounded by a protostellar disk, with a view toward applications to the formation of the Solar System. We use a specific model of star formation to demonstrate the possibility that such a star would become unstable, that the resulting distortion of the star would generate spiral density waves in the circumstellar disk, and that the torque associated with these waves would regulate the angular momentum of the star as it feeds angular momentum to the disk. We conclude that the angular momentum so transported to the disk would not spread the disk to, say, Solar System dimensions, by the action of the spiral density waves alone. However, a viscous disk could effectively extract stellar angular momentum and attain Solar System size. Our results also indicate that viscous disks could feed mass and angular momentum to a growing protostar in such a manner that distortions of the star would occur before gravitational torques could balance the influx of angular momentum. In other situations (in which the viscosity was small), a gap could be cleared between the disk and star.