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排序方式: 共有206条查询结果,搜索用时 109 毫秒
1.
现代腕足动物壳体的δ~(18)O和δ~(13)C值J。C。Scott等在过去的34年里,许多科技工作者利用古代腕足动物壳体的δ18值作为古海水δ18O值或温度的长期变化的指示物。最近,腕足动物壳体的δ18O值被用来计算古海水的温度、密度和盐度,以了解古... 相似文献
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Polar bears bioaccumulate lipophilic pollutants, including polychlorinated biphenyls (PCBs), into their bodies from their exclusive diet of marine organisms. Hydroxylated PCB metabolites (OH-PCBs) have been found in plasma, presumably due to CYP-dependent biotransformation of PCBs in liver. Little is known about the phase 2 metabolism of hydroxylated xenobiotics in polar bears. The objective of this study was to examine UDP-glucuronosyltransferase (UGT) activity with OH-PCBs and a hydroxylated polycyclic aromatic hydrocarbon, 3-hydroxy-benzo(a)pyrene (3-OH-BaP), in polar bear liver. Samples of frozen polar bear liver were used to prepare microsomes. UGT activity with 3-OH-BaP in Brij-treated microsomes, measured by a fluorescence assay, was readily measurable with protein concentrations in assay tubes of up to 10 g/ml, but dropped off very sharply at higher protein concentrations. The apparent Km for 3-OH-BaP was 1.71 +/- 0.04 microM, and Vmax 1.26 +/- 0.16 nmol/min/mg protein (mean +/- SD, n=3). UGT activities with a model tetrachloro-OH-PCB (4'-OH-CB72) and a model hexachloro-OH-PCB (4'-OH-CB159) were assayed with [14-C]-UDPGA and separation of the [14-C]-glucuronide by ion-pair extraction and thin-layer chromatography. [14-C]-glucuronide conjugates were readily formed by polar bear liver microsomes in the absence of added substrate, apparently from contaminants present in liver. This phenomenon was not observed using hepatic microsomes from laboratory-held catfish. Glucuronidation efficiency was much higher with 4'-OH-CB72 (Km 7.3 microM; Vmax 1.55 nmol/min/mg) than 4'-OH-CB159 (Km 16.1 microM; Vmax 0.46 nmol/min/mg). The identities of the aglycones present in polar bear liver are not known, but could include OH-PCBs or hydroxylated metabolites of other persistent organic pollutants. This study demonstrates that UGT with high activity for 3-OH-BaP and other substrates is present in polar bear liver. 相似文献
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The purpose of this research was to demonstrate a concentration effect of zinc exposure at organismal, cellular and sub-cellular levels in the eastern oyster and to find associated protein expression signatures (PES) for each concentration of zinc. Oysters were exposed to six concentrations of zinc for 48 h in a controlled environment. At the organismal level, fecal material was observed as a measure of physiological health during metal exposures. At the cellular level, lysosomal destabilization was measured using hemolymph. This cellular response was significant only at the highest concentration, when the fecal index was lowest. Protein responses were monitored in the oyster following exposure to zinc. Gill tissue was excised and homogenized, and then analyzed using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and digital image analysis. Protein expression signatures were found to be specific to each concentration. The protein responses were linked to the other biological parameters measured, each of which followed a concentration gradient of zinc. 相似文献
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Detailed investigations of high latitude sequences recently collected by the Ocean Drilling Program (ODP) indicate that periods of rapid climate change often culminated in brief transient climates, with more extreme conditions than subsequent long term climates. Two examples of such events have been identified in the Paleogene; the first in latest Paleocene time in the middle of a warming trend that began several million years earlier: the second in earliest Oligocene time near the end of a Middle Eocene to Late Oligocene global cooling trend. Superimposed on the earlier event was a sudden and extreme warming of both high latitude sea surface and deep ocean waters. Imbedded in the latter transition was an abrupt decline in high latitude temperatures and the brief appearance of a full size continental ice-sheet on Antarctica. In both cases the climate extremes were not stable, lasting for less than a few hundred thousand years, indicating a temporary or transient climate state. Geochemical and sedimentological evidence suggest that both Paleogene climate events were accompanied by reorganizations in ocean circulation, and major perturbations in marine productivity and the global carbon cycle. The Paleocene-Eocene thermal maximum was marked by reduced oceanic turnover and decreases in global delta 13C and in marine productivity, while the Early Oligocene glacial maximum was accompanied by intensification of deep ocean circulation and elevated delta 13C and productivity. It has been suggested that sudden changes in climate and/or ocean circulation might occur as a result of gradual forcing as certain physical thresholds are exceeded. We investigate the possibility that sudden reorganizations in ocean and/or atmosphere circulation during these abrupt transitions generated short-term positive feedbacks that briefly sustained these transient climatic states. 相似文献
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金沙江上游巴塘—中咱河段位于青藏高原东南缘,该河段两岸岸坡发育众多的大型古滑坡,且部分古滑坡曾堵塞金沙江形成了堰塞湖,特米大型古滑坡堰塞湖是其中之一。关于特米古滑坡堰塞湖的形成与演化过程目前尚未见有过详细的报道。本文在野外调查的基础上,结合遥感影像解译和年代学测试,对特米古滑坡堰塞湖的地貌和沉积特征进行了详细研究,并对其形成与演化过程进行了分析。研究结果表明,特米古滑坡堰塞湖很可能是由该地区的古地震活动触发大型滑坡并堵塞金沙江形成的,最大湖面面积约为1.42×107 m2,库容蓄水量约为1.46×109 m3。该古堰塞湖的形成时间约为1.8 ka BP,其溃决消亡的时间约为1.4 ka BP,溃决洪峰流量约为55 858 m3/s,该滑坡堰塞湖持续稳定了约400年的时间。 相似文献