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Glutathione S-transferase activity was measured in the hepatic cytosol front Dissostichus mawsoni and Pagothenia borchgrevinki. Activity measures with 1-chloro-2,4-dinitrobenzene as substrate were 11·2 and 16·7 μmol/min/g tissue respectively. Little or no activity was detected when p-nitrobenzyl chloride or 3,4-dichloro-1-nitrobenzene were used as substrate. The hepatic glutathione S-transferases from D. mawsoni were partially purified using gel filtration and chromatofocusing. Three peaks of activity were resolved. The major isozyme (158-fold purification) eluting at pH7·1 appeared to be catalytically a homodimer. The isozyme was highly inhibited by triphenyltin chloride (IC50 = 0·1 μ) while inhibition constants for Cibicron Blue 3GA, bromosulphophalein and hematin were 1·1, 20 and 34 μ respectively. 相似文献
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We present high resolution millimeter, near-infrared, and optical data on the Wolf-Rayet nucleus of the Liner NGC 6764. The millimeter12CO(1-0) maps were obtained using the Nobeyama Millimeter Interferometer. Near-infrared images in the K-band continuum and the 2.12µm H2, 2.06µm He I, 2.17µm Br, and 1.64µm [Fe II] lines were taken with the MPE imaging spectrometer FAST at the William Herschel Telescope on La Palma, Spain. The optical data were obtained at the 3.5m telescope on Calar Alto, Spain. The measurements indicate a strong concentration of molecular gas and a massive starburst at the nucleus of NGC 6764. The interferometric position velocity map of the nucleus shows the presence of distinct molecular cloud complexes with an apparently asymmetric velocity field shifted towards the blue with respect to the systemic velocity of 2420 km s–1. The distribution of the 2.12µm H2 line flux exhibits extensions approximately perpendicular to the bar which are in agreement with structural features in VLA radio maps and IRAM 30m maps of the12CO(2–1) line emission. This may represent evidence for combination of a nuclear outflow and a central oval distortion of gas predicted by gas dynamical calculations as a response to a bar like potential. A detailed investigation of the Wolf-Rayet-feature at 466 nm indicates that it is spatially extended on a scale of a few arcseconds. 相似文献