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排序方式: 共有507条查询结果,搜索用时 13 毫秒
1.
麻痹性贝毒(Paralytic Shellfish Poisoning,PSP)毒素由石房蛤毒素(saxitoxin,STX)及其衍生物组成,目前己发现20余种,在赤潮研究、分子生物学和神经生物学基础研究、医药、军事防化等方面都有应用潜力[其结构、类型和应用见本集刊王云峰等(2003)“麻痹性贝毒毒素的应用研究进展”一文]。由于PSP毒素的稀有来源和国际社会对STX交易的禁止,限制了国内PSP毒素应用及研究的全面深入展开,因此本文作者对PSP毒素进行了制备,并采用不同方法对制备的PSP毒素进行了研究。
PSP毒素能够选择性地可逆抑制可兴奋膜的电压依赖钠离子通道的开放,从而阻止神经冲动的发生和传导,使神经、肌肉丧失兴奋性(Frace et al.,1986;Penzotti et al.,1998)。本文利用神经束膜下记录和全细胞膜片钳技术,报道了从塔玛亚历山大藻(Alexandrium tamarense)中提取的PSP粗毒素对小鼠运动神经末梢膜电流和NG108-15细胞钠离子通道的作用研究结果,并与STX标准毒素的作用结果进行了比较。
NG108-15细胞是由小鼠神经母细胞瘤和大鼠胶质细胞瘤融合的杂交细胞,经分化剂分化后,显示诸+L1196如兴奋性、合成和释放乙酰胆碱、与培养肌细胞形成突触联系等多种神经细胞的基本特性(Hamprcht,1977)和Na+、K+、Ca2+等多种离子通道,已作为神经细胞模型被广泛应用于分析药物对离子通道作用的研究(Enomoto et al.,1992; Docherty et al.,1992;Shi et al.,1993;Hu et al.,1997a;Hu et al.,1997b);发育出具有Na+内流支持的锋电位(Hamprecht,1977),该电位是分析作用于膜钠离子通道药物的好材料。 相似文献
2.
The characteristics and sources of natural gases from Ordovician weathered crust reservoirs in the Central Gas Field in the Ordos Basin 总被引:2,自引:2,他引:0
Xianqing Li Guoyi Hu Jian Li Dujie Hou Peng Dong Zhihong Song Yunfeng Yang 《中国地球化学学报》2008,27(2):109-120
The Central Gas Field is a famous large-sized gas field in the Ordos Basin of China. However, identification of main gas sources of the Ordovician reservoirs in this gas field remains puzzling. On the basis of a lot of geochemical data and geological research on natural gases, the characteristics and sources of natural gases from Ordovician weathered crust reservoirs in the Central Gas Field in the Ordos Basin were studied. The results indicated that natural gases from Ordovician weathered crust reservoirs in the Central Gas Field in the Ordos Basin have similar chemical and isotopic compositions to highly mature and over-mature dry gases. Both coal-derived gases and oil-type gases coexist in the Central Gas Field in the Ordos Basin. The former was derived mainly from Carboniferous-Permian coal measures and the latter from Lower Paleozoic marine carbonates. It is suggested that coal-derived gases occur in the eastern part of the Central Gas Field while oil-type gases may be produced mainly in the northern, western and southern parts of the Central Gas Field in the Ordos Basin. 相似文献
3.
In the process of steam stimulation exploitation of viscous crude oil, the injected water, at high temperature and under high pressure, reacts intensively with the host rock. This kind of water–rock interaction in Liaohe Shuguang Oil Field was studied on the basis of analysis of water composition changes, laboratory experiments, mineral saturation indices analysis, and mass balance calculation. Compared with the injected water, the changes of the composition of discharged water are mainly the distinct decrease of pH, Na+, SiO2 and Cl−, as well as the increase of K+, Ca2+, Mg2+, SO
4
2−
and HCO
3
−
. Laboratory experiments under field conditions showed: the dissolution sequence of minerals quantitatively is quartz>potassium feldspar>albite, and the main change of clay minerals is the conversion of kaolinite to analcime. Mass balance calculation indicated during the process of steam stimulation, large quantities of analcime are precipitated with the dissolution of large amounts of quartz, kaolinite, potassium feldspar, and CO2. These results correlated very well with the experimental results. The calculated results of Liaohe Shuguang Oil Field showed that during the steam stimulation for viscous crude oil, the amounts of minerals dissolved (precipitated) are huge. To control the clogging of pore spaces of oil reservoirs, increased study of water–rock interaction is needed. 相似文献
4.
沁水盆地煤层气钻井工艺方法 总被引:1,自引:1,他引:1
针对沁水盆地煤层气概况,地层情况及开采条件,介绍了参数井、生产井、多分支地面煤层气水平开发井、丛式井等钻井工艺、钻具组合、井身结构及井身质量要求. 相似文献
5.
This paper presents a statistical performance analysis of a semi‐active structural control system for suppressing the vibration response of building structures during strong seismic events. The proposed semi‐active mass damper device consists of a high‐frequency mass damper with large stiffness, and an actively controlled interaction element that connects the mass damper to the structure. Through actively modulating the operating states of the interaction elements according to pre‐specified control logic, vibrational energy in the structure is dissipated in the mass damper device and the vibration of the structure is thus suppressed. The control logic, categorized under active interaction control, is defined directly in physical space by minimizing the inter‐storey drift of the structure to the maximum extent. This semi‐active structural control approach has been shown to be effective in reducing the vibration response of building structures due to specific earthquake ground motions. To further evaluate the control performance, a Monte Carlo simulation of the seismic response of a three‐storey steel‐framed building model equipped with the proposed semi‐active mass damper device is performed based on a large ensemble of artificially generated earthquake ground motions. A procedure for generating code‐compatible artificial earthquake accelerograms is also briefly described. The results obtained clearly demonstrate the effectiveness of the proposed semi‐active mass damper device in controlling vibrations of building structures during large earthquakes. Copyright © 2003 John Wiley & Sons, Ltd. 相似文献
6.
GPS折射角资料的变分同化试验 总被引:3,自引:0,他引:3
越来越多的新型观测资料为数值天气预报水平的进一步提高提供了许多新的机会。在各种新型的观测资料中,GPS(全球定位卫星系统)折射角资料无疑是非常重要的。GPS折射角资料具有分辨率高、全天候探测、覆盖全球等优点,实现对GPS折射角资料的变分同化,将具有非常重要的意义。文中介绍了如何获得及同化GPS折射角资料的原理。对GPS折射角资料的变分同化可以分为两种:间接同化和直接同化,文中对这两种方法都作了具体介绍。在变分同化的最小化过程中,计算效率无疑是最重要的,而优化步长的计算又直接关系到算法效率的成败。根据最小化算法的特点,通过数学推导,得出一种适合于各种最小化算法的计算优化步长的自适应方法。最后,还利用1995年10月11日的GPS折射角资料进行了数值试验,结果表明了变分同化方法和计算优化步长方法的有效性。 相似文献
7.
8.
9.
Junlai Liu Anjian Wang Haoran Xia Yunfeng Zhai Lan Gao Qunye Xiu Zhaochong Zhang Zhidan Zhao Dianhua Cao 《Mineralium Deposita》2010,45(6):567-582
There are two types of lead–zinc ore bodies, i.e., sandstone-hosted ores (SHO) and limestone-hosted ores (LHO), in the Jinding
giant sulfide deposit, Yunnan, SW China. Structural analysis suggests that thrust faults and dome structures are the major
structural elements controlling lead–zinc mineralization. The two types of ore bodies are preserved in two thrust sheets in
a three-layered structural profile in the framework of the Jinding dome structure. The SHO forms the cap of the dome and LHO
bodies are concentrated beneath the SHO cap in the central part of the dome. Quartz, feldspar and calcite, and sphalerite,
pyrite, and galena are the dominant mineral components in the sandstone-hosted lead–zinc ores. Quartz and feldspar occur as
detrital clasts and are cemented by diagenetic calcite and epigenetic sulfides. The sulfide paragenetic sequence during SHO
mineralization is from early pyrite to galena and late sphalerite. Galena occurs mostly in two types of cracks, i.e., crescent-style
grain boundary cracks along quartz–pyrite, or rarely along pyrite–pyrite boundaries, and intragranular radial cracks in early
pyrite grains surrounding quartz clasts. The radial cracks are more or less perpendicular to the quartz–pyrite grain boundaries
and do not show any overall (whole rock) orientation pattern. Their distribution, morphological characteristics, and geometrical
relationships with quartz and pyrite grains suggest the predominant role of grain-scale cracking. Thermal expansion cracking
is one of the most important mechanisms for the generation of open spaces during galena mineralization. Cracking due to heating
or cooling by infiltrating fluids resulted from upwelling fluid phases through fluid passes connecting the SHO and LHO bodies,
provided significant spaces for crystallization of galena. The differences in coefficients of thermal expansion between pyrite
and quartz led to a difference in volume changes between quartz grains and pyrite grains surrounding them and contributed
to cracking of the pyrite grains when temperature changed. Combined thermal expansion and elastic mismatch due to heating
and subsequent cooling resulted in the radial and crescent cracking in the pyrite grains and along the quartz–pyrite grain
boundaries. 相似文献
10.