Controls of δS and δO in dissolved sulphate: Learning from a detailed survey in the Llobregat River (Spain)

The S and O isotopic composition of dissolved SO₄, used as a tracer for SO₄ sources, was applied to the water of the Llobregat River system (NE Spain). The survey was carried out at 30 sites where surface water was sampled on a monthly basis over a period of 2a. The concentration of dissolved SO₄ varied from 20 to 1575 mg L⁻¹. Sulphur isotopic compositions clustered in two populations: one – 93% of the samples – had positive values with a mode of +9‰; the other had negative values and a mode of −5‰. Data for δ¹⁸O_SO4 showed a mean value of +11‰, with no bi-modal distribution, though lower values of δ¹⁸O corresponded to samples with negative δ³⁴S. These values can not be explained solely by the contribution of bedrock SO₄ sources: that is, sulphide oxidation and the weathering of outcrops of sulphates, though numerous chemical sediments exist in the basin. Even in a river with a high concentration of natural sources of dissolved SO₄, such as the Llobregat River, the δ³⁴S values suggest that dissolved SO₄ is controlled by a complex mix of both natural and anthropogenic sources. The main anthropogenic sources in this basin are fertilizers, sewage, potash mine effluent and power plant emissions. Detailed river water sampling, together with the chemical and isotopic characterisation of the main anthropogenic inputs, allowed determination of the influence of redox processes, as well as identification of the contribution of natural and anthropogenic SO₄ sources and detection of spatial variations and seasonal changes among these sources. For instance, in the Llobregat River the input of fertilisers is well marked seasonally. Minimum values of δ³⁴S are reported during fertilization periods – from January to March – indicating a higher contribution of this source. The dual isotope approach, δ³⁴S and δ¹⁸O, is useful to better constrain the sources of SO₄. Moreover, in small-scale studies, where the inputs are well known and limited, the mixing models can be enhanced and the contribution of the different sources can be quantified to some extent.     

南海西部海域新生代构造古地理演化及其对油气勘探的意义

南海西部海域构造复杂,主要发育有北东—北东东向、北西向和近南北向3组深大断裂。其中,北西向断裂与板块汇聚、碰撞有关,多具走滑性质;北东—北东东向断裂具有与中国东部裂谷盆地相似的发育特点,呈张扭性质;近南北向断裂可能是南海在扩张活动期间于洋、陆壳过渡部位形成的走滑调节断裂,是洋盆扩张的西部边界。新生代里,南海经历了4次成盆事件与3期扩张活动,盆地经历了古新世—中始新世陆缘断陷、渐新世—早中新世扩展与中中新世以来的热沉降3个演化阶段。陆缘断陷阶段的充填系列主要是北东—北东东向与北西向的河流—冲积扇、湖泊沼泽等陆相沉积及火山岩等;盆地扩展阶段表现为中-小型断陷、断-坳陷逐渐复合与联合为大-中型坳陷,古地理格局逐渐由河流与湖沼陆相环境演变为滨海至浅海相的沉积环境;热沉降阶段的成盆活动逐渐减弱以至停止,地层表现超覆,盆地出现联合迹象。结合以往勘探与油气资源调查成果分析,认为南海西部海域陆架陆坡区发育的大-中型沉积盆地石油地质条件良好,蕴藏着丰富的油气资源,勘探潜力巨大。     

超声波对盐藻的破碎作用

通过实验和对超声波破碎盐藻作用机制分析,为从盐藻中提取胡萝卜素探求一种简便的新途径。     

温度、pH和金属离子对仿剌参蛋白酶活力影响的研究

利用分光光度计比色法测定了温度、pH值和Hg^2＋等9种金属离子对体长24．2cm±0．7cm仿刺参（Apostichopus japonicus）消化道中蛋白酶活性的影响。结果表明，蛋白酶的最适温度为40℃，对热不稳定，最适pH为7．2。Hg^2＋，Mn^2＋，Ag^＋，Pb^2＋，Ba^2＋，Ca^2＋对蛋白酶的活性有抑制作用，抑制作用由强至弱依次为Hg^2＋〉Ag^＋〉Mn^2＋〉Pb^2＋〉Ca^2＋〉Ba^2＋，其中Hg^2＋和Ag^＋与对照差异极显著（P〈0．01），表现出极强的抑制作用；Cu^2＋，Zn^2＋，Mg^2＋则对酶活有一定的促进作用，其促进作用大小依次为Mg^2＋〉Zn^2＋〉Cu^2＋，Mg^2＋与对照差异显著（P〈0．05）。     

羊栖菜多糖提取与含量的测定

通过水提醇沉方法提取羊栖菜粗多糖,纯化后测定羊栖菜中多糖含量,为今后羊栖菜化学及药理研究提供有益的参考.采用了分光光度法、苯酚-硫酸显色法在490 nm处测定其含量.结果表明,羊栖菜提取液中多糖含量达53.46%,平均多糖回收率为99.37%.证明该方法实验操作简便,准确可靠,可用于羊栖菜多糖含量的测定.     

Genetic Relationships Among One Non-Endemic and Two Endemic Mediterranean Triplefin Blennies (Pisces, Blennioidei)

Abstract. Three triplefin blennies occur sympatrically in the Mediterranean Sea; Tripterygion tripteronotus and T. melanurus are endemic, whereas T. delaisi is also found in the Eastern Atlantic. Although very similar in morphology, ecology and behaviour, some striking differences exist among reproductive strategies. Several authors proposed hypotheses on the evolution of these species. In order to enhance insight into the genetic structure of this genus, an enzyme electrophoretic survey was performed. Samples of 40 to 45 specimens of each species were collected near Calvi (Corsica, France). Twenty-four enzyme loci were analysed, of which 17 appeared polymorphic. T. delaisi showed a high level of average observed heterozygosity (H_o) of 0.140, as opposed to very low levels of 0.009 and 0.021 in T. tripteronotus and T. melanurus , respectively. Nei's genetic distance was 0.21 between T. delaisi and T. tripteronotus , 0.35 between T. delaisi and T. melanurus and 0.45 between T. tripteronotus and T. melanurus . We hypothesise that divergence of these species started before the Pleistocene and that the endemic species survived the Pleistocene glaciations in refugia within the Mediterranean. The possibility that T. tripteronotus and T. delaisi diverged through sympatric speciation is discussed.