Accelerator mass spectrometry radiocarbon dating of 1994 Lake Winnipeg cores

Conventional radiocarbon dating of Lake Winnipeg core samples has produced erroneously old ages due to the incorporation of pre-Quaternary carbon derived from carbonaceous rocks, soils and sediments in the watershed, as well as a hard-water effect resulting from leaching of calcareous rocks and soils. To circumvent these problems and develop a reliable chronology for the Lake Winnipeg core series, a total of 64 samples from the Lake Winnipeg core series were processed to isolate well preserved macrofossils suitable for Accelerator Mass Spectrometry (AMS) radiocarbon dating. Here we report six radiocarbon ages derived from plant macrofossils and ostracodes, and reconstruct aspects of the depositional environment of each sample based on the associated macrofossil assemblage.     

Anthropogenic changes in the sediment composition of Lake Gości& aogon;ż (central Poland), during the last 330 yrs*

According to historical sources, the development of settlement around Lake Goci& aogon; during the last 330 yrs was intense at two time periods: the second half of the 1700's, and from ca. 1880 until 1944. The small farms were then abandoned, following which the lake surroundings were planted with forest trees. The presented study of human influence on the lake ecosystem and surrounding vegetation has been based on analyses of general sediment composition and its chemistry, pollen, Cyanobacteria, Chlorophyceae, Rotatoria, Cladocera, and a preliminary diatom survey. The history of human impact has been divided into four phases: 1. Phase of small local hamlets (before ca. 1770): The human impact was rather moderate then, but the cultivation of Canabis sativa, Secale cereale and later of Fagopyrum is evidenced from that time. 2. Phase of Hollandii settlement (ca. 1770-1863). Its influence is indicated first by the recession of deciduous wood (Corylus, Carpinus) stands, which triggered drastic drop of calcium in sediments. The development of rural economy in the area, including, an extension of agriculture (Secale and other cereals, crucifers, potatoes), and animal breeding based partly on grazing in the forest, is evidenced only after 1820. 3. Phase of German colonization (1863-1944): In the early periods (before 1910) the pollen spectra do not document any essential change in the type of farming, however, a serious disturbance of the lake ecosystem and sediment chemistry is evidenced by the blooms of Araphidinae diatoms and Tetraedron minimum, a maximum frequency of Bosmina longirostris, disturbances of the regular spring blooms of Chrysophyceae, appearance of vivianite, distinct maxima of organic matter, potassium, and iron concentration in sediments and an increase of the sedimentation rate. An intensification of agricultural activities commenced around 1910; woods, including also pinewoods, were then heavily devastated, and farming extended on poor soils, what was symptomatic for the general poverty of population. Coincidently in the lake, Centriceae showed blooms, Araphidinae diatoms and Tetraedron minimum developed, and the content of potassium, iron and phosphorus increased substantially, indicating altogether rising eutrophication. 4. Phase of restoration of the natural landscape (after 1945): The farm degradation from ca. 1944 is very weakly expressed in pollen data, which show a substantial fall of farming indicators from 1953/6 only, when the whole area was used for forest plantation. It was accompanied by a certainly spontaneous development of Betula and Alnus woods, this process progressing till recent time. The gradual extinction of farming activity near Lake Goci was accompanied by abrupt changes in the lake ecosystem, expressed by the restored blooms of Chrysophyceae expansion of planktonic Cladocera, rapid decline of phosphorus and extinction of vivianite from sediments. As documented by the drop of Cu/Zn ratio, lake hypolimnion has been weakly oxidized since 1949, what was probably responsible for the drop of Fe and Mn content in sediments. Increasing strength of overturns affected preservation of laminae in sediments, which almost completely disappeared after 1966.     

Hubbert’s petroleum production model: an evaluation and implications for World Oil Production Forecasts

Following Hubberts successful prediction of the timing of US peak oil production, Hubberts model has been used extensively to predict peak oil production elsewhere. However, forecasts of world and regional peak oil and natural gas production using Hubberts methodology usually have failed, leading to the implicit belief that such predictions always will fail and that we need not worry about finite resources. A careful examination of Hubberts approach indicates that the most important reasons for his success in the US were stable markets, the high growth rate of demand, ready availability of low cost imports, and a reasonable estimate of easily extractable reserves. This analysis also shows that his model cannot predict ultimate oil reserves and that it should be considered an econometric model. Building on Hubberts vital insight, that cheap fossil fuel reserves are knowable and finite, one can state that for world peak oil production, political constraints should be much more important than resource constraints.     

Zipf’s Law: A Viable Geological Paradigm?

Zipfs Law originally was proposed as a guide to a statistical distribution in social studies. The law describes a relationship between size and rank of discrete phenomena. It is a variant of Paretos 1927 Law known as the 80/20 rule and is similar to Bodes Law in concept. The relationship described by Zipfs Law is a succession of order data with the largest followed by half the size for the next largest, which in turn, the next is half that size, and so on. In geology, it has been used with moderate success in resource assessment of mining and petroleum. In essence, it predicts how many entities of a certain size may be left in a sequence of decreasing size assuming the largest has been ascertained. Examples of applications would be plotting the rank and size of ore deposits or oil fields to determine how many deposits remained undiscovered and their size. After a flurry of papers in the 1970s and 1980s, application of the law apparently either was successful and thus not reported in way the literature or was determined to be ineffectual and its use discontinued, but either way the law lapsed into obscurity. Examples of oil- and gas-field size in Kansas, the occurrence of historic earthquakes that affected the state, and size of anticlines (plains-type folds) are presented to illustrate application and limits of Zipfs Law.     

Oxygen isotope ratios of organic matter in arctic lakes as a paleoclimate proxy: field and laboratory investigations

Paleoclimate research based on the stable isotopic composition of lake sediments is often hampered by the lack of preservation of suitable material for isotopic analysis. We examined organic material as a proxy for past water isotopic composition in a series of experiments. First, we cultured aquatic moss under constant illumination, temperature, and water ¹⁸O, and show that new cellulose records source water ¹⁸O precisely (r² = 0.9997). Second, we analyzed paired lakewater and vegetation samples collected from sites spanning strong climatic gradients. In field conditions, the relationship between organic ¹⁸O and water ¹⁸O is more variable, though it is still controlled by environmental water isotopic composition. However, terrestrial mosses in the arctic are often significantly enriched in ¹⁸O relative to aquatic mosses in nearby lakes due to their use of different water sources. Third, we measured ¹⁸O of cellulose extracted from disseminated sedimentary organic material. In the majority of the middle- to high-arctic lakes in this study, the ¹⁸O of disseminated sediment cellulose is greatly enriched relative to the expected values based on lakewater ¹⁸O, suggesting a significant component of terrestrial cellulose. This interpretation is supported by radiocarbon dates from a Holocene sediment core in which ¹⁴C ages of sediment cellulose are 700-5000 yrs older than the enclosing sediments. We conclude that aquatic cellulose can be used as a reliable tracer of lakewater isotope ratios, but terrestrial cellulose often dominates the sedimentary cellulose pool in places such as Baffin Island where sedimentation rates are low enough to allow the degradation of aquatic cellulose. Care must be taken when interpreting sediment cellulose ¹⁸O records where diagenesis has played a role, because terrestrial cellulose is more resistant to degradation, and therefore can predominate in environments with low organic carbon burial.     

Lacustrine organic matter and the Holocene paleoenvironmental record of Lake Albano (central Italy)

A combined bulk and detailed geochemical study of the sedimentary organic matter in Lake Albano, central Italy, provides critical data to track the response of this aquatic system to the environmental changes of variable amplitude that occurred during the Holocene. RockEval pyrolysis of this predominantly laminated, organic carbonrich sedimentary sequence shows changes in hydrogen and oxygen indices that are related to variations in the dominance of the primary producers. These variations are further confirmed by the pigments and the carbon isotopic composition of bulk organic matter showing that cyanobacteria dominated the lake waters during the early and late Holocene whereas diatoms have been the main producers during the middle Holocene. Sharp decreases in productivity, 2–3 centuries long, are identified at ca. 8.2, 6.4 and 3.8 ka. B.P. Changes in temperature and/or effective moisture are suggested as the most probable causes, although human impact cannot be ruled out for the latest part of the Holocene.     

Stable isotopic compositions of sulfur in PIRLA sediment cores

Stable isotopic compositions of total sedimentary sulfur were determined for cores from eight of the PIRLA study lakes. Preindustrial sulfur deposited prior to 1800 had roughly constant isotopic compositions within a 1–2 range in each core. In more recent sediments, large 5–10 decreases in ³⁴S content occurred and likely resulted from the addition of anthropogenic sulfur. These isotopic changes began at the same time that lead concentrations increased in sediments, suggesting a close linkage between lead and sulfur deposition in the mid and late 1800s.This is the ninth of a series of papers to be published by this journal which is a contribution of the Paleoecological Investigation of Recent Lake Acidification (PIRLA) project. Drs. D.F. Charles and D.R. Whitehead are guest editors for this series.     

Sub-sampling unconsolidated sediments: A solution for the preparation of undistrubed thin-sections from clay‐rich sediments

Using the principles of electroosmosis, a subsampling technique for clay-rich sediments is introduced that produces undisturbed thinsections from sediment cores. The fragile sample remains in the same disposable box throughout the process, preventing collapse of its structure during subsampling, manipulation and impregnation, as well as other potential problems.     

On the red coloration of saltern crystallizer ponds. II. Additional evidence for the contribution of halobacterial pigments

In a previous paper we attempted to assess the contribution of red bacteria of theHalobacterium — Haloferax — Haloarcula group and of the -carotene-rich green algaDunaliella salina to the red colour of saltern crystallizer ponds. By means of light absorption measurements, we showed that bacterioruberin contained in the bacteria was mainly responsible for the colour of the brines, in spite of the fact that -carotene derived fromDunaliella was the pigment present in the greatest amount. This apparent discrepancy was explained by the very smallin vivo optical cross-section of -carotene, which is densely packed in globules inside theD. salina cells. We recently observed that the centrifugation technique used in the previous study to collect biomass from the ponds was unsuitable for this type of measurements, as a substantial part of theDunaliella cells present did not sediment upon centrifugation due to the low specific gravity caused by the high -carotene content. Therefore similar measurements were performed with biomass collected by filtration. Again,in vivo absorption spectra were dominated by the absorption peaks of bacterioruberin. The results reported here show that, in spite of the methodological problem associated with the earlier study, all views and conclusions expressed in our earlier paper retain their validity.     

Diatom and chrysophyte functions and inferences of post-industrial acidification and recent recovery trends in Killarney lakes (Ontario, Canada)

The surface sediment diatom and chrysophyte assemblages from 33 Sudbury lakes were added to our published 72 lake data set to expand and refine the diatom and chrysophyte-based inference models that we had earlier developed for this region. Our calibration data set now includes 105 lakes, representing gradients for multiple environmental variables (e.g., lakewater pH, metals, and transparency). The revised models are based on the weighted averaging calibration and regression approach and include bootstrap error estimates. The pH model was the strongest (r² _boot = 0.75, RMSE _boot = 0.50). The chrysophyte-inferred pH model (r² _boot = 0.79, RMSE _boot = 0.48) that we developed was as robust as the diatom pH model. Diatom and chrysophyte inferred pH models were then applied to top (surface sediments representing current conditions) and bottom (generally from > 30 cm deep representing pre-industrial conditions) sediment diatom and chrysophyte assemblages of 19 Killarney area lakes near Sudbury. The top and bottom inferred pH results were compared to early-1970s measured pH data. These data suggest that, although many of the poorly buffered Killarney lakes had experienced acidification, marked pH recovery has occurred in many lakes within the last 25 years. Despite the stunning pH recovery, the present-day diatom and chrysophyte assemblages are significantly different from assemblages present during pre-industrial times. Our results suggest that biological recovery may require more time than chemical recovery. It is also likely that these lakes may never recover biologically because other anthropogenic stressors (e.g., climate warming and increased exposure to UV-B radiation) may now have greater influence on biological communities in Killarney/Sudbury area lakes than acidification.     

Regional assessment of long-term hypolimnetic oxygen changes in Ontario (Canada) shield lakes using subfossil chironomids

Subfossil chironomid assemblages were used to infer long-term water quality changes in south-central Ontario shield lakes, which are currently being impacted by anthropogenic eutrophication, acid rain, and recent climate change. Using a transfer function developed to infer average end-of-summer volume-weighted hypolimnetic oxygen (avg VWHO), a top-bottom paleolimnological approach was used to reconstruct pre-industrial (pre-1850) deepwater oxygen conditions. Comparison with present-day (top surface sediments) chironomid-based inferences of avgVWHO results suggest that hypolimnetic oxygen levels are presently similar to natural, pre-industrial (bottom sediments) levels in most lakes. Approximately half of the study lakes recorded an increase in hypolimnetic oxygen since the 19th century. Inferred changes in avgVWHO correlate well with our results from another chironomid-based oxygen model which reconstructs the Anoxic Factor (AF). When study lakes are separated according to their hydrological status (i.e., natural versus managed), lakes with a dam at their outlet and seasonally managed lake levels had significantly different changes in avgVWHO compared to lakes with natural hydrology. Lakes with a dam at their outlet generally recorded increases in avgVWHO, while natural hydrology lakes mostly recorded declines. There was no relationship between inferred changes in avgVWHO and the density of cottage and resort development along the shorelines of lakes. Changes in dissolved organic carbon (DOC) possibly related to recent climate changes may also be affecting deepwater oxygen, however patterns of change are very subtle.     

Organo-geochemical and stable isotope indicators ofenvironmental change in a marl lake, Malham Tarn, North Yorkshire,U.K.

Sediments of the marl lake Malham Tarn located in NW Englandpreserve an environmental record since 12 Ka. Eight Holocene pollen zones wereidentified, and the ¹³C of total organic carbon (TOC) showsthree stratigraphic divisions. The basal clay unit and overlayingsand/clay/marl unit have ¹³C of –24which decreases at the base of the principal marl unit to a mean value around–30, whilst the topmost black marl unit ¹³Cincreases to –28 at the surface. Representative samples of theseunits were selected for analysis of n-alkanes andn-fatty acids and their ¹³C.Samples of modern Chara and peat were analysed forcomparison. The clay unit has a minor contribution of redeposited matureorganic matter and autochthonous algae, the marl unit a high contribution ofChara, and the dark marl unit has a high contribution fromhigher plants. Compound-specific ¹³C revealssystematic differences between alkanes and fatty acids of different chainlength. The major shift in ¹³C in the short and medium chainfatty acids are probably due to the decreasing influence of carbonate rockflour as source of DIC. The major shift in ¹³C in the longchain n-fatty acids andn-alkanes could reflect the lower atmosphericCO₂ concentration at Last Glacial. The negative shift of short chainfatty acids in organic rich dark marls reflects introduction of detrital peatinto the lake. The ¹³C results show a dramatic change fromdominance of autochthonous plus eroded sources up to Pollen Zone IV, then slowcolonisation of the hinterland by higher plants, followed by constantChara contributions throughout the deposition of the marl,and a further increase of higher plant material after the rise in water levelin 1791.     

Quantitative inferences of past hypolimnetic anoxia and nutrient levels from a Canadan Precambrian Shield lake

Paleolimnological analyses were used to infer limnological changes during the past ~ 300 yrs in the west basin of Peninsula Lake, a small (853 ha) Precambrian Shield lake in Ontario, Canada, that has been subjected to moderate cultural disturbances (forest clearance, cottage and resort development). This study represents a pioneering attempt to use sedimentary chironomid assemblages and weighted-averaging models to quantify past hypolimnetic anoxia (expressed as the anoxic factor, AF). Impacts of forest clearance and human land-use on deepwater oxygen availability and surface water quality were assessed by comparing chironomid-inferred AF and diatom-inferred total phosphorus concentration ([TP]) to changes in terrestrial pollen and historical data. This study also discusses the ability of chironomids to quantitatively infer changes in AF.Pre-disturbance chironomid assemblages were stable and dominated by taxa indicative of oxygen-rich hypolimnetic conditions (e.g., Protanypus, Heterotrissocladius, Micropsectra type), while diatoms indicated oligotrophic lake status (diatom inferred [TP] = 5-7 g·l^-1). Chironomids characteristic of lower oxygen availability (e.g., Chironomus, Procladius) increased following land-clearance, road construction, establishment of a grist mill and lakeshore development beginning ca. 1870. Increased abundances of Tanytarsus s. lat., a multigeneric group of mainly littoral chironomids, since 1900, indicated that littoral chironomids may have comprised a greater proportion of fossil assemblages during periods of eutrophication and prolonged anoxia. Abundances of meso-eutrophic diatom taxa (e.g., Fragilaria crotonensis, Asterionella formosa, Aulacoseira ambigua, A. subarctica) increased concurrent with European settlement (ca. 1870) and diatom-inferred [TP] doubled (~ 6-12 g·l^-1), further indicating that naturally-oligotrophic Precambrian Shield lakes were extremely sensitive to initial land-clearance activities.Recent increases in oligotrophic diatom taxa (e.g., Cyclotella stelligera) indicate a shift to more oligotrophic conditions since ca. mid-1960s, with greatest changes since ca. 1980. The chironomids Heterotrissocladius and Micropsectra type also increased at this time suggesting greater deepwater oxygen availability. These recent water-quality improvements, possibly in response to enhanced nutrient removal from detergents and sewage, climate-related reductions in external phosphorus loads, and catchment (but not lake) acidification and reforestation, suggest that habitat for commercially-valuable cold-water fishes has improved in recent decades despite greater recreational lake-use.Paleolimnological assessment of trophic status changes in Peninsula Lake using fossil diatom and chironomid assemblages were in good agreement. Diatom inferences of [TP] and chironomid inferences of AF both suggest that Peninsula Lake was historically oligotrophic, became oligo-mesotrophic after European settlement, and returned to oligotrophy in recent yrs. Chironomid inferences of [TP] consistently underestimated the trophic status of Peninsula Lake, possibly due to its relatively large hypolimnion. These results suggest that AF represents a useful tool for quantitatively reconstructing the past trophic status of deeper, stratified lakes.     

The influence of flushing rates, terrestrial input and low salmon escapement densities on paleolimnological reconstructions of sockeye salmon (Oncorhynchus nerka) nutrient dynamics in Alaska and British Columbia

Recent advances in paleolimnology have enabled reconstructions of past sockeye salmon (Oncorhynchus nerka) dynamics using a number of proxy-indicators, including diatoms and stable isotopes. Thus far, studies have focused on nursery lakes with high escapement densities and low flushing rates, ensuring that levels of salmon-derived nutrients (SDN) are high and are incorporated into the food chain. This study examines three oligotrophic sockeye salmon nursery lakes in Alaska (Afognak and Saltery lakes) and British Columbia (Hobiton Lake) to determine if sockeye salmon populations can be tracked in nursery systems with lower salmon escapement densities, higher flushing rates and/or higher terrestrial input. We adopted a multi-proxy approach using diatoms, stable isotopes (¹⁵N), organic carbon to nitrogen (C/N) ratios and pollen to draw inferences from ²¹⁰Pb-dated sediment cores. ¹⁵N showed little response to historic variation in sockeye salmon populations, even in Saltery Lake, which has a very high escapement density, and in Afognak Lake, in which average escapement is known to have increased. Dilution effects due to high flushing rates were likely partly responsible for the low ¹⁵N and minimal variation throughout the cores, although very high terrestrial input in Hobiton Lake also dampened the salmon signal. Small changes in diatom species assemblages, however, were evident in all three lakes and may be in response to fluctuating loads of salmon-derived nutrients. Most notably, increases of mesotrophic diatom taxa, such as Asterionella formosa and Aulacoseira subarctica, corresponded to increased salmon production in Alaskan lakes as a result of enhancement (fertilization) activities and climatic changes. Changes in the relative abundance of Cyclotella pseudostelligera in Hobiton Lake may also be in response to a significant decline in sockeye salmon populations off the west coast of Vancouver Island in the 1970s. Other factors, however, such as logging and lake fertilization may also have influenced diatom species composition. These results confirm that, while salmon-derived nutrients may be of key importance in juvenile salmonid development in some lakes, this may not be the case in all systems, especially those in which flushing rates are high. Further, in these systems, diatom communities appear to respond more sensitively to fluctuations in salmon populations (and therefore nutrients) than stable isotope methods, provided that other changes in trophic status are minor.     

Diatoms and sockeye salmon (Oncorhynchus nerka) population dynamics: Reconstructions of salmon-derived nutrients over the past 2,200 years in two lakes from Kodiak Island, Alaska

The return of hundreds to millions of adult sockeye salmon (Oncorhynchus nerka), which have returned from the ocean to their natal nursery lake environment to spawn, can result in significant nutrient loading. By analyzing sedimentary diatom assemblages from nursery lakes, we demonstrated that a salmon-derived nutrient signal could be traced over time and be used to infer past sockeye salmon population dynamics. We conducted a 2,200 year paleolimnological study of two Alaskan sockeye salmon nursery lakes, Karluk and Frazer lakes. The two lakes are very similar, except that sockeye salmon were only introduced into Frazer Lake in 1951 (first spawners returned in 1956). In both lakes we found a strong correspondence between diatom assemblages and the number of adult salmon spawners recorded in the historical data (40 and 70 years for Frazer and Karluk lakes, respectively). Given this robust relationship, we then used our analyses of diatoms from Karluk Lake over the past 2,200 years to gain insight into salmon-derived nutrient loading changes (which are directly related to the number of sockeye salmon spawners). The diatom record from Karluk Lake recorded dramatic species changes on both decadal and century timescales, and was strongly correlated with an independent indicator of sockeye salmon abundances, ¹⁵N. Together, these data suggest pronounced variability in sockeye salmon abundances at Karluk Lake over the past 2,200 years. The direct impacts of regional environmental variability were not likely responsible for the patterns apparent in Karluk Lake, as the diatom and ¹⁵N profiles from Frazer Lake were relatively stable prior to the introduction of sockeye salmon. Application of total phosphorus transfer functions to the Karluk and Frazer lakes' diatom records revealed that sockeye salmon carcasses substantially increased the trophic status in these lakes, which has important implications for the health of juvenile salmon that rear in nursery lakes. Overall, this paper illustrates the potential use of diatoms in reconstructing past sockeye salmon population dynamics, which in turn can lead to a greater understanding of the mechanisms influencing abundances of sockeye salmon.     

Diatom-based ionic concentration and salinity models from the south Bolivian Altiplano (15–23 ° S

The relationship between surface sediment diatom assemblages and measured limnological variables in fifty eight lake samples from the south Bolivian Altiplano was examined by constructing a diatom-water chemistry dataset. Analysis of this dataset by canonical correspondence analysis revealed that salinity and ionic concentration accounted for a significant amount of the variation in the distribution of the diatom assemblages. Two methods weighted-averaging regression and calibration, and by-class mean percentage table were used to established a transfer function for future reconstruction of past lake water salinity and ionic concentration in the southern Bolivian Altiplano. Weighted-averaging regression and calibration with inverse deshrinking provided a better model for the water chemistry reconstructions in this region.     

Quaternary changes in delivery and accumulation of organic matter in sediments of Lake Biwa, Japan

A 911-m-long sediment core from Lake Biwa, Japan, provides a record of organic matter delivery and accumulation in this large lake during a succession of tectonic and climatic changes dating back to the latest Pliocene. Sediments deposited since 430 ky are profundal; older sediments vary in setting between shallow-water and fluviodeltaic conditions, with occasional deep-water intervals. C/N ratios identify algal production as the dominant source of organic matter throughout the core, although the proportion of land-derived contributions episodically increases in the fluviodeltaic and shallow-water sediments. Rates of organic matter delivery and burial in lake sediments change in response to glacial-interglacial climate changes over the past 430 ky. Sediments deposited during interglacial intervals have organic carbon mass accumulation rates up to 9 times greater than those from glacial intervals, reflecting interglacial climates that were wetter than glacial climates. Algal production of organic matter increased during interglacial times because of greater wash-in of soil nutrients, and organic matter preservation was enhanced because of faster sedimentation rates.     

Seasonal temperatures for the past ∼400 years reconstructed from diatom and chironomid assemblages in a high-altitude lake (Lej da la Tscheppa,Switzerland)

We analysed a 42 cm long sediment record from Lej da la Tscheppa, a high-altitude lake (2,616 m a.s.l.) in the Upper Engadine valley (Switzerland) for subfossil diatoms, chironomids and pollen. The chronology of the top 21 cm of the record was established using ²¹⁰Pb analysis using a constant-rate-of-supply model, and validated with ¹³⁷Cs measurements and the content of spheroidal carbonaceous particles. A tentative chronology for the lower part of the core was obtained through extrapolation of the sedimentation rates in the uppermost part of the record. Pollen assemblages in the record reflect regional changes in forestation and land-use patterns in the Upper Engadine valley and show no evidence of significant local human activity in the lake’s catchment. Diatom assemblages record a distinct increase in planktonic taxa since the early 19th century, suggesting a decrease in the duration of ice-cover. In contrast, chironomid assemblages remained stable during a large part of the record. We applied an established chironomid-based July air temperature transfer function and a newly developed diatom-based spring air temperature transfer function to reconstruct past seasonal air temperature changes at Lej da la Tscheppa. The reconstructions indicate a diatom-inferred warming trend in spring temperatures during the past ca. 400 years, whereas chironomid-inferred summer temperatures suggest a slight cooling trend. These biota-based reconstructions are in good agreement with the centennial-scale temperature trend in an independent reconstruction of regional temperatures in the Upper Engadine region based on instrumental records and documentary proxy evidence from the Alps. Our results suggest that, in high-altitude lakes, independent chironomid- and diatom-based seasonal temperature reconstruction is possible and can be successfully used to track seasonal temperature trends.     

Chrysophyte cysts as paleolimnological indicators of environmental change due to cottage development and acidic deposition in the Muskoka-Haliburton region, Ontario, Canada

Chrysophyte cysts preserved in recent and pre-industrial lake sediment samples from 54 Muskoka-Haliburton (Ontario) lakes were used in a paleolimnological study to determine the impact of acidic precipitation and cottage development on water quality. A total of 246 cyst morphotypes were identified. Ecological preferences of cyst morphotypes were determined using multivariate statistical analysis, cluster analysis, and species-environment correlations. Recent cyst assemblages were related to water chemistry and lake morphometric variables using Redundancy Analysis (RDA). The distribution of morphotypes was related to a gradient of acid neutralising capacity (ANC), expressed through the association of variables related to buffering (i.e. longitude, watershed area, and ionic concentration) with the first axis (₁ = 0.29). Cyst assemblages were also defined, to a lesser extent (₂ = 0.06), by a trophic status gradient, created through the combination of total nitrogen (TN), total phosphorus (TP), volume-weighted cottage density, and lake depth variables. The identification of lakewater pH and trophic status as important determinants of cyst assemblage structure allowed for the reconstruction of acidification and eutrophication related water chemistry changes using fossil cyst assemblages. The reconstruction of pre-industrial (pre-1850) water quality conditions with fossil cyst assemblages indicated that pH significantly decreased in 24.1% of the study lakes and increased in 16.7% of the lakes. Increases in pH in more alkaline drainage basins are attributed to alkalinity generation processes induced by acidic precipitation as has been shown in other studies. Total phosphorus (TP) concentrations significantly declined in 12.9% of the lakes and increased in 16.6% of lakes. Increases in [TP] were linked to cottage development. Decreases in trophic status may be due to landuse changes, the result of the acidification occurring in the area, or warmer and drier climates. A comparison of chrysophyte cyst and diatom water quality inferences show similar trends in pH changes. There is a good agreement between diatom and chrysophyte bioindicators with respect to [TP] changes in oligotrophic lakes (< 10 g/L); however, diatom inferences suggest that lakes with current [TP] values greater than 10 g/L have decreased in trophic status over time, while chrysophyte reconstructions suggest that these same lakes have become more productive systems.