Hydrolysis Activities of the Particle of Agarose-Ce^4＋ Complex for Compounds Containing Phosphodiester or Peptide Bonds

Hydrolysis activities of PACC （particle of agarose-Ce^4＋ complex, newly made through double emulsification） for compounds containing phosphodiester or peptide bonds were studied. The results showed that PACC could hydrolyze organophosphorous pesticides not only in water but also in vegetable juice or tea extract. Hydrolysis rates of methamidophos, omethoateand chlorpyrifos in water are 32.39%, 27.12% and 46.62% respectively, those of chlorpyrifos and methamidophos in mung sprout juice 38.28% and 35.45% respectively, and that of chlorpyrifos in tea extract 59.76%. Hydrolysis rates of BSA （bovine serum albumin） in water and protein in tea extract by PACC increase by 54.30% and 86.46% respectively as compared with the control.     

基于GEE云平台的福建省10 m分辨率茶园专题空间分布制图

福建省作为中国的产茶大省,快速准确获取茶园的空间分布对福建省农业经济发展和生态环境保护具有重要的决策意义,然而,传统的方法难以保证大范围准确地获取茶园空间分布。本文基于GEE云平台,快速获取覆盖福建省的Sentinel-1雷达影像、Sentinel-2光学影像及地形数据,从中提取光谱特征、纹理特征、地形特征等98个特征,利用递归消除支持向量机算法（SVM_RFE）对特征变量进行筛选,通过支持向量机分类器（SVM）进行茶园提取,首次得到福建省2019年10 m分辨率茶园种植区空间分布图。结果表明：① 光谱特征在茶园信息提取中占据重要地位,纹理特征和地形特征次之;② 利用SVM_RFE可以有效筛选出最有利于茶园提取的特征子集,有效提高提取精度,总体精度为94.65%,Kappa系数为0.93,茶园的生产者精度为91.64%,用户精度为92.91%;③ 基于Sentinel-1及Sentinel-2影像获取的福建省2019年茶园种植面积为1913 km²,主要分布在安溪县、福鼎市、福安市、武夷山市和寿宁县,其茶园总面积达910 km²,约占据全省茶园面积的48%。利用云计算技术可以克服大尺度茶园监测运算能力不足的问题,结合Sentinel-1和Sentinel-2影像能够较准确地提取福建省茶园分布,对南方丘陵山区茶园及其他作物提取具有参考价值,并为政府及有关部门进行茶园管理提供支持。     

Spatiotemporal Variation of Riverine Nutrients in a Typical Hilly Watershed in Southeast China Using Multivariate Statistics Tools

The water quality of lakes can be degraded by excessive riverine nutrients.Riverine water quality generally varies depending on region and season because of the spatiotemporal variations in natural factors and anthropogenic activities.Monthly water quality measurements of eight water quality variables were analyzed for two years at 16 sites of the Tianmuhu watershed.The variables were examined using hierarchical cluster analysis(HCA) and factor analysis/principal component analysis(FA/PCA) to reveal the spatiotemporal variations in riverine nutrients and to identify their potential sources.HCA revealed three geographical groups and three periods.Two drainages comprising towns and large villages were the most polluted, six drainages comprising widely distributed tea plantations and orchards were moderately polluted, and eight drainages without the factors were the least polluted.The river was most polluted in June when the first heavy rain(daily rainfall 50 mm) occurs after fertilization and the number of rainy days is most(monthly number of rainy days 20 days).Moderate pollution was observed from October to May, during which morethan 60% of the total nitrogen fertilizer and all of the phosphorus fertilizer are applied to the cropland, the total manure is applied to tea plantations and orchards, and a monthly rainfall ranging from 0 mm to 164 mm occurs.The remaining months were characterized by frequent raining(i.e., number of rainy days per month ranged from 5 to 24) and little use of fertilizers, and were thus least polluted.FA/PCA identified that the greatest pollution sources were the runoff from tea plantations and orchards,domestic pollution and the surface runoff from towns and villages, and rural sewage, which had extremely high contributions of riverine nitrogen, phosphorus,and chemical oxygen demand, respectively.The tea plantations and orchards promoted by the agricultural comprehensive development(ACD) were not environmentally friendly.Riverine nitrogen is a major water pollution parameter in hilly watersheds affected by ACD, and this parameter would not be reduced unless its loss load through the runoff from tea plantations and orchards is effectively controlled.     

胡萝卜花生乳发酵酸奶工艺研究

以胡萝卜花生仁为主要原料,经加工处理、调配后进行乳酸发酵,通过正交试验确定其最适的工艺条件。结果表明：当花生乳：胡萝卜汁为2：1,奶粉用量为2％,糖用量为12％,固形物：水为1：9,接种量为2％,前发酵温度为30℃或35℃,前发酵时间为10～12h或8～10h时,所制得胡萝卜花生乳发酵酸奶的凝乳状态、口感、色泽、风味最好。     

The Anti-allergic Activity of Polyphenol Extracted from Five Marine Algae

Natural polyphenol has been widely believed to be effective in allergy remission. Currently, most of the natural polyphenol products come from terrestrial sources such as tea, grape seeds among others, and few polyphenols have been developed from algae for their anti-allergic activity. The aim of the study was to screen some commercial seaweed for natural extracts with anti-allergic activity. Five algae including Laminaria japonica, Porphyra sp., Spirulina platensis, Chlorella pyrenoidosa and Scytosiphon sp. were extracted with ethanol, and the extracts were evaluated for total polyphenol contents and anti-allergic activity with the hyaluronidase inhibition assay. Results showed that the total polyphenol contents in the ethanol extracts ranged from 1.67% to 8.47%, while the highest was found in the extract from Scytosiphon sp. Hyaluronidase inhibition assay showed that the extracts from Scytosiphon sp. had the lowest IC50, 0.67 mg m L-1, while Chlorella pyrenoidosa extract had the highest IC50, 15.07 mg m L-1. The anti-allergic activity of Scytosiphon sp. extract was even higher than the typical anti-allergic drug Disodium Cromoglycate(DSCG)(IC50 = 1.13 mg m L-1), and was similar with natural polyphenol from Epigallocatechin gallate(EGCG)(IC50 = 0.56 mg m L-1). These results indicated that the ethanol extract of Scytosiphon sp. contains a high concentration of polyphenol with high anti-allergic activity. Potentially Scytosiphon sp. can be developed to a natural anti-allergic compound for allergy remission.     

基于改进SVM分类法的SAR图像水体面积提取研究

在复杂地表环境下的多云多雨地区,基于合成孔径雷达（SAR）图像提取水体时容易受到其它地物如水田、山体阴影等干扰,传统的灰度阈值法和SVM法未能考虑水体与其它地物在纹理和地形上的差异,因此水体提取结果精度较差。研究首先用Refined Lee滤波对SAR图像进行预处理;其后通过DEM建模和坡度计算提取地形特征,通过计算图像灰度共生矩阵以提取纹理特征（包括均匀性、角二阶矩和熵）,并结合SAR图像极化信息以及SDWI指数形成针对水体提取的特征空间,通过融合地形特征和图像纹理特征发展了改进SVM分类法的水体提取模型。在使用Sentinel-1 SAR数据对所发展模型与SDWI水体指数法、传统SVM法水体提取结果进行比对后发现,改进SVM分类法提取的水体结果较好地剔除了水田和山体阴影,且提取的水体水面比传统的SVM法更加完整;该方法在总体精度、Kappa系数、漏分率和错分率指标上均优于SDWI法和传统的SVM法,总体精度达到98.06%,比SDWI法和传统的SVM法分别提高了23.24%和5.49%,有效提高了复杂环境下地表水体的提取精度。研究最后将所发展模型应用于2018年马哈韦利河流域逐月水...     

浙江长兴顾渚山优质紫笋茶与地质背景相关性分析

长兴水口顾渚山风景区的紫笋茶,是闻名国内外的优质名茶。本文在实地调查的基础上,分析了紫笋茶优异的品质与地质环境背景条件的相关性。特定的地质背景条件,首先造就了一个避风向阳、雨量充沛的小气候环境;特定的泥盆系与花岗岩类浸入岩的地层背景所形成的土壤,为茶树生长提供了大量的营养物质,特别是有益的微量元素对提高茶叶品质起到了重要作用;同时这一特定的背景条件下,形成了对茶树生长有利的酸性土壤。特定的水文地质背景条件．又提供了充足的水份,还补充了茶树生长所必需的营养物质。通过对环境背景条件的分析,最终提出了长兴县茶叶发展的方向。     

Screening of Extraction Methods for Glycoproteins from Jellyfish （Rhopilema esculentum） Oral-Arms by High Performance Liquid Chromatography

In order to select an optimum extraction method for the target glycoprotein (TGP) from jellyfish (Rhopilema esculentum) oralarms, a high performance liquid chromatography (HPLC)–assay for the determination of the TGP was developed. Purified target glycoprotein was taken as a standard glycoprotein. The results showed that the calibration curves for peak area plotted against concentration for TGP were linear (r = 0.9984, y = 4.5895x+47.601) over concentrations ranging from 50 to 400 mgL-1. The mean extraction...     

Screening of extraction methods for glycoproteins from jellyfish (Rhopilema esculentum) oral-arms by high performance liquid chromatography

In order to select an optimum extraction method for the target glycoprotein (TGP) from jellyfish (Rhopilema esculentum) oral-arms, a high performance liquid chromatography (HPLC)-assay for the determination of the TGP was developed. Purified target glycoprotein was taken as a standard glycoprotein. The results showed that the calibration curves for peak area plotted against concentration for TGP were linear (r = 0.9984, y = 4.5895x+47.601) over concentrations ranging from 50 to 400 mgL-1. The mean extraction recovery was 97.84% (CV2.60%). The fractions containing TGP were isolated from jellyfish (R. esculentum) oral-arms by four extraction methods: 1) water extraction (WE), 2) phosphate buffer solution (PBS) extraction (PE), 3) ultrasound-assisted water extraction (UA-WE), 4) ultrasound-assisted PBS extraction (UA-PE). The lyophilized extract was dissolved in Milli-Q water and analyzed directly on a short TSK-GEL G4000PWXL (7.8 mm×300 mm) column. Our results indicated that the UA-PE method was the optimum extraction method selected by HPLC.     

江浙典型茶园土壤有机质空间异质性分析

茶园土壤有机质分布有明显的空间性,如何凭借有限的采样点数据预测研究区的有机质分布,对了解茶叶种植区土壤状况、指导茶园经营管理、提高茶叶品质和产量有重要意义。本文对江浙4种名茶种植区,利用描述性统计,以及地统计学中的半方差函数模型、空间插值等方法对比分析了茶园土壤有机质的空间异质性。研究结果表明:(1)4个研究区的土壤有机质平均水平均较高,适合茶叶生长,且浙江省2个茶叶种植区的有机质平均水平高于江苏省2个茶叶种植区的有机质水平;(2)半方差函数模型拟合表明,溪龙乡种植区有机质含量表现出中等空间相关性,而其他3个种植区表现出强烈的空间相关性;(3)各种植区土壤有机质的空间分布以结构性因子(土壤理化性质、地形地貌)为基础,受人类活动、经营模式、茶园管理等随机性因子影响,整体趋势上表现出局部变异的特征。     

Soil respiration under three different land use types in a tropical mountain region of China

Soil respiration (SR) Wis one of the largest contributors of terrestrial CO_2 to the atmosphere.Environmental as well as physicochemical parameters influence SR and thus, different land use practices impact the emissions of soil CO_2. In this study, we measured SR, bi-monthly, over a one-year period in a terrace tea plantation, a forest tea plantation and a secondary forest, in a subtropical mountain area in Xishuangbanna, China. Along with the measurement of SR rates, soil characteristics for each of the land use systems were investigated. Soil respiration rates in the different land use systems did not differ significantly during the dry season, ranging from2.7±0.2 μmol m~(-2) s~(-1) to 2.8±0.2 μmol m~(-2) s~(-1). During the wet season, however, SR rates were significantly larger in the terrace tea plantation(5.4±0.5 μmol m~(-2)s~(-1)) and secondary forest(4.9±0.4 μmol m~(-2)s~(-1)) than in the forest tea plantation(3.7±0.2 μmol m~(-2) s~(-1)).This resulted in significantly larger annual soil CO_2 emissions from the terrace tea and secondary forest,than from the forest tea plantation. It is likely that these differences in the SR rates are due to the 0.5times lower soil organic carbon concentrations in thetop mineral soil in the forest tea plantation, compared to the terrace tea plantation and secondary forest.Furthermore, we suggest that the lower sensitivity to temperature variation in the forest tea soil is a result of the lower soil organic carbon concentrations. The higher SR rates in the terrace tea plantation were partly due to weeding events, which caused CO_2 emission peaks that contributed almost 10% to the annual CO_2 flux. Our findings suggest that moving away from heavily managed tea plantations towards low-input forest tea can reduce the soil CO_2 emissions from these systems. However, our study is a casestudy and further investigations and upscaling are necessary to show if these findings hold true at a landscape level.     

基于水化学数据的矿山涌水水源识别:主成分分析与残差分析

涌水是矿山安全生产的重要威胁之一,掌握涌水的水源是预防这类灾害的重要依据。基于多期次的水化学监测数据,提出了利用主成分分析(PCA)和残差分析来识别涌水的水源数量和水源类型。主成分分析能够充分提取高维数据的结构信息,消除变量冗余。残差分析利用主成分分析结果,提取不同的主成分个数重构离子浓度,得出重构离子浓度和原始离子浓度之间的残差,利用残差的结构性信息来判断可能的涌水水源数量和水源类型。结果表明:随保留主成分个数的增加,重构离子浓度的精度越高,离子残差的结构性得到消除且表现出随机性。在实例应用中,通过利用5个主成分重构离子浓度消除了所有主要离子残差结构性,保证了信息的最大程度提取,因此,确定研究矿山涌水水源数量为5个;同时,利用每个主成分对残差结构性的影响程度及离子相关性分析,确定了这5个水源类型为海水、第四系孔隙水、富钙基岩水、富镁基岩水和淡水。     

Purification and photodynamic bioactivity of phycoerythrin and phycocyanin from Porphyra yezoensis Ueda

Phycoerythrin and phycocyanin were purified from Porphyra yezoensis Ueda with their bioactivity determined in this study. Continuous precipitation with ammonium sulfate at different concentrations （10%, 20%, 40%and 50%） increased the purity （A564：A280） of phycoerythrin to 1.49, 3.92 fold of the raw extract （0.38） and the purity （A615：A280） of phycocyanin to 0.70, 3.33 fold of the raw extract （0.21）. Two more times of chromatography with hydroxylapatites finally made the purity of phycoerythrin and phy-cocyanin reach 5.50, 14.47 fold of the raw extract, and 5.10, 24.29 fold of the raw extract, respectviely. The yield of high purity phycoerythrin and phycocyanin were 0.21%and 0.09%of dried P. yezoensis blade, respectively. The photodynamic cytotoxic ex-periment showed that both phycoerythrin and phycocyanin inhibited the growth of liver tumor cells significantly. It was found that 250 mg L-1 purified phycoerythrin and phycocyanin inhibited the growth of hepatocellular carcinoma cells 24 h after laser-irradiation by 80%and 59%, respectively, and 100 mg L-1 purified phycoerythrin and phycocyanin induced the apoptosis of 31.54%and 32.54%of the cells, respectively, 8 h after photodynamic therapy. Oue findings demonstrated that P. yezoensis can serve as photosensitizer （phycoerythrin and phycocyanin） producer.     

ALUMINUM CONTENT OF TEA LEAVES AND FACTORSAFFECTING THE UPTAKE OF ALUMINUM FROMSOIL INTO TEA LEAVES

1 INTRODUCTIONAluminum, the most abundant metallic elementwithin the lithosphere (7% -- 8% by weight), occursgenerally only in combined form: as an oxide inban-cite, the primary ore, and in complex aluminosilicates sueh as micas and feldspars (HEM, 1986;LINDSAY, 1979). These compounds of Al, nearlyunavailable to plants, dissolve to form the hydrated ionAl(H,O),' (written A13 for simplicity) or hydrolysisproducts of this ion under acidic conditions. The Alions bind to canon-excha…     

Distribution of Aluminum and Fluoride in Tea Plant and Soil of Tea Garden in Central and Southwest China

The distribution of Al and F contents and the relationship between Al and F in tea plants and soils of 12 tea gardens in Central and Southwest China were investigated from October 31 to November 14, 2006. The results show that there were differences in pH, CEC, the contents of organic matter (OM), Al and F in the different soils of the tea gardens. The Al content ranged from 1196 to 7976mg/kg for old leaf, 370 to 2681mg/kg for young leaf and 285 to 525mg/kg for stem, whereas the content of F ranged from 221 to 1504mg/kg for old leaf, 49 to 602mg/kg for young leaf and 13.5 to 77.5mg/kg for stem. The concentrations of labile Al varied obviously in the different soils, but the distribution law of labile Al content for the same garden was Alexchangeable≈AlFe-Mn oxide>Alorganic>Alwater-soluble. The contents of different labile F fractions varied slightly in the different soils and the different soil layers, though the exchangeable F content was lowest among the labile F in the soils. The concentrations of Al and F in tea plants increased with increasing amount of water-soluble Al or F, especially the amount of water-soluble fractions in the soil layer of 0-20cm.The correlation between Al content and F content in the tea leaf was more significant than that in the tea stem. Furthermore, the correlation between Al content and F content in whole tea plant was strongly significant (r=0.8763, p<0.01, n=36). There were evident tendency that Al concentration increased with the increase of F concentration in different soil layers. The correlation of water-soluble Al with water-soluble F in all soils was also strongly significant (r=0.7029, p<0.01, n=34). The results may provide a proof that Al and F are jointly taken up by tea plants to some extent in natural tea gardens.     

基于离散粒子群算法的2种新型水体提取方法的对比与验证

随着遥感技术在水体提取与监测方面的广泛应用,更多的研究者致力于提高遥感水体提取的精度。离散粒子群算法在遥感图像分类研究中获得了较高的精度和更稳健的分类效果,已经被应用到遥感水体提取领域,但其在水体提取中的适用性和精度还有待对比与验证。本文采用最新提出的2种基于离散粒子群算法的水体提取方法,即光谱匹配耦合离散粒子群算法（SMDPSO）与最大熵耦合离散粒子群算法（MEDPSO）,基于Landsat8_OLI遥感影像,分别选择了有冰雪、有云、有山体阴影和有建筑物的4种环境复杂,常规方法提取精度较低的区域进行水体提取,并与2种常用的水体指数法（NDWI、MNDWI）进行了对比与验证。结果表明：① SMDPSO和MEDPSO方法在4个实验区都能快速地寻找出最佳的水体分布,具有一定的通用性;NDWI和MNDWI方法对有冰雪、有云、有山体阴影和有建筑物影响的区域表现出水体信息的错分现象,提取精度较低;② SMDPSO方法能够识别细小河流和离散水体,水体提取精度较高,但在有冰雪、云、山体阴影和建筑物的复杂环境下提取精度较低、误判率高;MEDPSO方法不仅可以识别细小水体,而且也解决了其他3种方法在提取过程中无法抑制背景信息干扰的问题,在4个实验区的总体精度均在97.8%以上,高于其他3种方法;③ 将离散粒子群算法引入到水体提取方法之中,可增强方法的区域整体性,也可提高其水体提取的精度和自动化程度;④ 运用最大熵模型等机器学习方法,可以结合光谱、形状和纹理等影像信息以及地形信息来进行水体识别,使得水体信息提取精度更高。本文的研究可为离散粒子群算法的推广以及遥感水体提取方法的选择提供参考。     

Growth inhibition to three red tide microalgae by extracts of Ulva pertusa

1 INTRODUCTION In recent decades, red tides that induced by in- creasing eutrophication occur frequently in coastal areas worldwide. This has caused serious problems in marine environment, aquaculture industries and human health (Qi et al., 1993; Horner e…     

基于改进U-Net网络的高分遥感影像水体提取

传统基于光谱信息的水体提取未能考虑水体形状、纹理、大小、相邻关系等问题,且存在同物异谱、异物同谱现象,导致水体提取精度较低。而传统基于分类提取水体方法设计特征过程较为繁琐,且不能挖掘深度信息特征。因此,本文提出改进的U-Net网络语义分割方法,借鉴经典U-Net网络的解编码结构对网络进行改进：① 将VGG网络用于收缩路径以提取特征;② 在扩张路径中对低维特征信息进行加强,将收缩特征金字塔上一层的特征图与下一层对应扩张路径上的特征图进行融合,以提高提取结果分割精度;③ 在分类后处理中引入条件随机场,以将分割结果精细化。在保持相同训练集、验证集和测试集的情况下,分别用SegNet、经典U-Net网络和改进的U-Net网络做对照试验。试验结果表明,改进的U-Net网络结构在IoU、精准率和Kappa系数指标上均高于SegNet和经典U-Net网络,与SegNet相比,3项指标分别提升了10.5%、12.3%和0.14,与经典U-Net网络结果相比,各个指标分别提升了5.8%、4.4%和0.05。改进的网络水体提取结果较为完整,对小目标水体能够准确提取。改进的U-Net网络能够有效地实现水体提取任务。     

淮山提取液替代羧甲基纤维素钠作为酸奶稳定剂的效果研究

采用正交试验等方法对淮山提取液（DB-Ext）代替羧甲基纤维素钠(CMC)作为调配型酸奶稳定剂的效果进行了初步探讨。结果表明以原料的6.0倍水量在110℃的温度条件下，提取40min，所得的淮山提取液的粘度为3500mPa     

水库重力测量检测非牛顿引力的实验数据分析新方法

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