Effects of seasonal variation on soil microbial community structure and enzyme activity in a Masson pine forest in Southwest China

Soil microbial communities and enzyme activities play key roles in soil ecosystems. Both are sensitive to changes in environmental factors,including seasonal temperature, precipitation variations and soil properties. To understand the interactive mechanisms of seasonal changes that affect soil microbial communities and enzyme activities in a subtropical masson pine(Pinus massoniana) forest, we investigated the soil microbial community structure and enzyme activities to identify the effect of seasonal changes on the soil microbial community for two years in Jinyun Mountain National Nature Reserve, Chongqing, China. The soil microbial community structure was investigated using phospholipid fatty acids(PLFAs). The results indicated that a total of 36 different PLFAs were identified, and 16:0 was found in the highest proportions in the four seasons, moreover, the total PLFAs abundance were highest in spring and lowest in winter. Bacteria and actinomycetes were the dominant types in the study area. Seasonal changes also had a significant(P 0.05) influence on the soil enzyme activity. The maximum and minimum values of the invertase and catalase activities were observed in autumn and winter, respectively. However, the maximum and minimum values of the urease and phosphatase acid enzymatic activities were found in spring and winter, respectively. Canonical correspondence analysis(CCA) analysis revealed that the seasonal shifts in soil community composition and enzyme activities were relatively more sensitive to soil moisture and temperature, but the microbial community structure and enzyme activity were not correlated with soil pH in the study region. This study highlights how the seasonal variations affect the microbial community and function(enzyme activity)to better understand and predict microbial responses to future climate regimes in subtropical area.     

Effects of seasonal water-level fluctuation on soil pore structure in the Three Gorges Reservoir,China

Inundation of the Three Gorges Reservoir has created a 30-m water-level fluctuation zone with seasonal hydrological alternations of submergence and exposure, which may greatly affect soil properties and bank stability. The aim of this study was to investigate the response of soil pore structure to seasonal water-level fluctuation in the reservoir, and particularly, the hydrological change of wetting and drying cycles. Soil pore structure was visualized with industrial X-ray computed tomography and digital image analysis techniques. The results showed that soil total porosity(? 100 ?m), total pore number, total throat number, and mean throat surface area increased significantly under wetting and drying cycles. Soil porosity, pore number and throat numberwithin each size class increased in the course of wetting and drying cycles. The coordination number, degree of anisotropy and fractal dimension were indicating an increase. In contrast, the mean shape factor, pore-throat ratio, and Euler-Poincaré number decreased due to wetting and drying cycles. These illustrated that the wetting and drying cycles made soil pore structure become more porous, continuous, heterogeneous and complex. It can thus be deduced that the water-level fluctuation would modify soil porosity, pore size distribution, and pore morphology in the Three Gorges Reservoir, which may have profound implications for soil processes, soil functions, and bank stability.     

Effects of vegetation coverage and seasonal change on soil microbial biomass and community structure in the dry-hot valley region

Soil microorganisms are sensitive indicator of soil health and quality. Understanding the effects of vegetation biomass and seasonal change on soil microorganisms is vital to evaluate the soil quality and implement vegetation restoration. This study analyzed the soil phospholipid fatty acids (PLFAs) in fresh and withered Kudzu (Pueraria montana var. lobata) vegetation conditions in different seasons. The results showed that vegetation biomass and seasonal change significantly affected microbial biomass and its community structure. Both fresh and withered Kudzu cover significantly increased soil microbial biomass, and the growth effect of microbes in the soil with fresh Kudzu cover was more obvious than that with withered Kudzu cover. Compared with the dry season, the rainy season significantly increased the microbial biomass and the B/F (the ratio of bacterial to fungal PLFAs) ratio but dramatically reduced the G+/G- (the ratio of gram-positive to gram-negative bacteria PLFAs). Kudzu cover and seasonal change had a significant effect on microbial structure in soil covered by higher vegetation biomass. Furthermore, soil temperature and moisture had different correlations with specific microbial biomass in the two seasons. Our findings highlight the effect of Kudzu vine cover on the soil microenvironment and soil microhabitat, enhancing the soil quality in the Dry-hot Valley of Jinsha River, Southwest China.     

Spatial and seasonal variation in soil respiration along a slope in a rubber plantation and a natural forest in Xishuangbanna,Southwest China

Soil respiration is a key component of the global carbon cycle, and even small changes in soil respiration rates could result in significant changes in atmospheric CO₂ levels. The conversion of tropical forests to rubber plantations in SE Asia is increasingly common, and there is a need to understand the impacts of this land-use change on soil respiration in order to revise CO₂ budget calculations. This study focused on the spatial variability of soil respiration along a slope in a natural tropical rainforest and a terraced rubber plantation in Xishuangbanna, Southwest (SW) China. In each land-use type, we inserted 105 collars for soil respiration measurements. Research was conducted over one year in Xishuangbanna during May, June, July and October 2015 (wet season) and January and March 2016 (dry season). The mean annual soil respiration rate was 30% higher in natural forest than in rubber plantation and mean fluxes in the wet and dry season were 15.1 and 9.5 Mg C ha^-1 yr^-1 in natural forest and 11.7 and 5.7 Mg C ha^-1 yr^-1 in rubber plantation. Using a linear mixed effects model to assess the effect of changes in soil temperature and moisture on soil respiration, we found that soil temperature was the main driver of variation in soil respiration, explaining 48% of its seasonal variation in rubber plantation and 30% in natural forest. After including soil moisture, the model explained 70% of the variation in soil respiration in natural forest and 76% in rubber plantation. In the natural forest slope position had a significant effect on soil respiration, and soil temperature and soil moisture gradients only partly explained this correlation. In contrast, soil respiration in rubber plantation was not affected by slope position, which may be due to the terrace structure that resulted in more homogeneous environmental conditions along the slope. Further research is needed to determine whether or not these findings hold true at a landscape level.     

Field capacity in black soil region,Northeast China

In this work, 23 black soil profiles were surveyed and 113 soil samples were collected to determine the field capacity (FC) of the black soil in Northeast China. The effectiveness of three methods measuring FC, the Wilcox method (WM), the undisturbed soil pressure plate method (PUM) and the air-dried sieved soil pressure plate method (PDM) were compared to select a suitable laboratory measurement method. Results show that the FC values measured by PDM are greater than those measured by PUM, and the values m...     

Analysis of seasonal variation of water masses in East China Sea

Seasonal variations of water masses in the East China Sea （ECS） and adjacent areas are investigated, based on historical data of temperature and salinity （T-S）. Dynamic and thermodynamic mechanisms that affect seasonal variations of some dominant water masses are discussed, with reference to meteorological data. In the ECS above depth 600 m, there are eight water masses in summer but only five in winter. Among these, Kuroshio Surface Water （KSW）, Kuroshio Intermediate Water （KIW）, ECS Surface Water （ECSSW）, Continental Coastal Water （CCW）, and Yellow Sea Surface Water （YSSW） exist throughout the year. Kuroshio Subsurface Water （KSSW）, ECS Deep Water （ECSDW）, and Yellow Sea Bottom Water （YSBW） are all seasonal water masses, occurring from May through October. The CCW, ECSSW and KSW all have significant seasonal variations, both in their horizontal and vertical extents and their T-S properties. Wind stress, the Kuroshio and its branch currents, and coastal currents are dynamic factors for seasonal variation in spatial extent of the CCW, KSW, and ECSSW, whereas sea surface heat and freshwater fluxes are thermodynamic factors for seasonal variations of T-S properties and thickness of these water masses. In addition, the CCW is affected by river runoff and ECSSW by the CCW and KSW.     

The community structure and seasonal dynamics of plankton in Bange Lake,northern Tibet,China

The seasonal variations in biomass, abundance, and species composition of plankton in relation to hydrography were studied in the saline Bange Lake, northern Tibet, China. Sampling was carried out between one to three times per month from May 2001 to July 2002. Salinity ranged from 14 to 146. The air and water temperature exhibited a clear seasonal pattern, and mean annual temperatures were approximately 4.8°C and 7.3°C, respectively. The lowest water temperature occurred in winter from December to March at-2°C and the highest in June and July at 17.7°C. Forty-one phytoplankton taxa, 21 zooplankton, and 5 benthic or facultative zooplankton were identifi ed. The predominant phytoplankton species were Gloeothece linearis, Oscillatoria tenuis, Gloeocapsa punctata, Ctenocladus circinnatus, Dunaliella salina, and Spirulina major. The predominant zooplankton species included H olophrya actra, Brachionus plicatilis, Daphniopsis tibetana, Cletocamptus dertersi, and A rctodiaptomus salinus. The mean annual total phytoplankton density and biomass for the entire lake were 4.52×10~7 cells/L and 1.60 mg/L, respectively. The annual mean zooplankton abundance was 52, 162, 322, and 57, 144 ind./L, in the three sublakes. The annual mean total zooplankton biomass in Lakes 1–3 was 1.23, 9.98, and 2.13 mg/L, respectively. The annual mean tychoplankton abundances in Bg1, 2, and 3 were 47, 67, and 654 ind./L. The annual mean tychoplankton biomass was 2.36, 0.16, and 2.03 mg/L, respectively. The zooplankton biomass(including tychoplankton) in the lake was 9.11 mg/L. The total number of plankton species in the salt lake was signifi cantly negatively correlated with salinity.     

Variation law of microscopic pore of loess-like soil after several freeze-thaw cycles in seasonal frozen soil region

In seasonal frozen soil region,the engineering geological properties of loess-like soil will be deteriorated after freeze-thaw cycles.Through the freeze-thaw cycle experiment of remolded loess-like soil,under different freezing temperatures,the authors carried out freeze-thaw cycle tests for 3 times and 20 times,respectively.With mercury intrusion porosimetry and granulometric analysis,from the micro-structure,the authors studied the law that freeze-thaw cycle times and frozen temperature effect on the variation of microscopic pore of loesslike soil.This result can provide theoretical basis for comprehensive treatment of problems in the construction of the project in seasonal frozen loess-like soil region.     

Spatial-temporal variation of phytoplankton community structure in Jiaozhou Bay,China

To better understand the spatial-temporal variation in phytoplankton community structure and its controlling factors in Jiaozhou Bay,Qingdao,North China,four seasonal sampling were carried out in 2017.The phytoplankton community structure and various environmental parameters were examined.The phytoplankton community in the bay was composed of mainly diatoms and dinoflagellates,and several other species of Chrysophyta were also observed.Diatoms were the most dominant phytoplankton group throughout the year,except in spring and winter,when Noctiluca scintillans was co-dominant.High Si/N ratios in summer and fall reflect the high dominance of diatoms in the two seasons.Temporally,the phytoplankton cell abundance peaked in summer,due mainly to the high temperatures and nutrient concentrations in summer.Spatially,the phytoplankton cell abundance was higher in the northern part of the bay than in the other parts of the bay in four seasons.The diatom cell abundances show significant positive correlations with the nutrient concentrations,while the dinoflagellate cell abundances show no correlation or a negative correlation with the nutrient concentrations but a significant positive correlation with the stratification index.This discrepancy was mainly due to the different survival strategies between diatoms and dinoflagellates.The Shannon-Wiener diversity index(H')values in the bay ranged from 0.08 to 4.18,which fell in the range reported in historical studies.The distribution pattern of H' values was quite different from that of chlorophyll a,indicating that the phytoplankton community structure might have high biomass with a low diversity index.Compared with historical studies,we believe that the dominant phytoplankton species have been changed in recent years due mainly to the changing environment in the Jiaozhou Bay in recent 30 years.     

Integrated indicators-based gradation of cultivated land quality in mountainous region of Southwestern China

1 Introduction The gradation of cultivated land is to assess the suitability of cultivated land for agricultural production in terms of natural and economic prop-erties of land (Feng et al. 2004). It can be used to evaluate sustainability of land use and …     

Diversity and seasonal variation of marine phytoplankton in Jiaozhou Bay,China revealed by morphological observation and metabarcoding

Journal of Oceanology and Limnology - Phytoplankton are central components of marine environments, and are major players in the production and respiration budgeting. However, their diversity and...     

Modelling spatial variation in the treatment costs of nonpoint source pollution in mountainous regions of southwest China

Non-point source(NPS) pollution is considered to be one of the main threats of the aquatic environment. Mountainous regions are particularly important water sources for urban areas. The various driving factors of NPS pollution such as terrain, precipitation, and vegetation type in mountainous regions show clear spatial heterogeneity. Consequently, the management systems required for NPS pollution in mountainous regions are complex. In this study, we developed a framework to estimate and map the treatment costs for NPS pollution in mountainous regions and applied this method in Baoxing County, a typical mountainous county in Sichuan Province of southwest China. The export levels of total nitrogen(TN) and total phosphorus(TP) in Baoxing County were estimated using the water purification model in InVEST(Itegrated Valuation of Ecosystem Services and Tradeoffs) tool. NPS pollutant treatment costs were calculated based on the level of pollutants exports, water yield, water quality targets, and treatment costs of NPS pollutants per unit mass. The results show that at the watershed level the amounts of TN and TP exported in Baoxing County were below threshold limits. However, at the sub-watershed level, TN and TP excesses of 291.64 and 2.96 tons per year were found, respectively, with mean TN and TP treatment costs of 6.58 US$/hm~2 and 0.35 US$/hm~2. Appraising pollution treatment cost intuitively reflects the overall expenditure in NPS pollution reduction from an economic perspective. This study provides a foundation for the implementation of Payment for Ecosystem Service(PES) and the prevention and control of NPS pollution.     

Spatiotemporal variation of soil organic carbon in the cultivated soil layer of dry land in the South-Western Yunnan Plateau,China

The dynamics of soil organic carbon(SOC)in cropland is one of the central issues related to both soil fertility and environmental safety. However, little information is available at county level regarding the spatiotemporal variability of SOC in the southwestern mountainous region of China. Thus, this study aimed to explore spatiotemporal changes of SOC in the cultivated soil layer of dry land in Mojiang County,Yunnan Province, China. Data were obtained from the second national soil survey(SNSS) of 1985 and soil tests for fertilizer application carried out by the Mojiang Agricultural Bureau in 2006. The ANOVA test was applied to determine any significant differences between the datasets, while semivariogram analysis was performed on geostatistics via an ordinary Kriging method in order to map spatial patterns of soil organic carbon density(SOCD). The results revealed that SOCD in the cultivated soil layer significantly decreased from 3.93 kg m~(-2) in 1985 to 2.89 kg m~(-2) in 2006, with a total soil organic carbon stock(SOCS) decrease of 41.54×10~4 t over the same period. SOCS levels fell most markedly in yellow-brown soil at a rate of51.52%, while an increase of 8.70% was found in the analysed latosol. Geostatistical analysis also showed that the recorded changes in SOCD between 1985 and2006 were spatially structured. The decreasing trend might be attributed to the combined action of intense cultivation, major crop residue removal without any protective tillage measures, unreasonable fertilization and natural climatic diversity inducing a large decrease in SOC in the studied cultivated dry land region of Mojiang County. Therefore, management measures such as protective tillage should be undertaken in order to enhance soil C sequestration.     

Dynamic characteristics of seasonal thermocline in the deep sea region of the South China Sea

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Relationship between stand characteristics and soil properties of two typical forest plantations in the mountainous area of Western Sichuan,China

There is uncertainty and limited knowledge regarding stand characteristics and soil properties of middle-aged pure forest plantations in the mountainous area of Western Sichuan. Plantation forests are almost exclusively planted as monocultures in the Pengzhou forest farm in the western mountainous area of Sichuan Province. This study aimed to assess the spatial and ecological characteristics of middle-aged Cunninghamia lanceolata(Lamb.) Hook. and Cryptomeria fortunei Hooibr. in the Pengzhou forest farm. The relationships and differences between stand growth characteristics and forest soil composition were assessed on the two forests planted simultaneously within the same area and exposed to the same environmental conditions. Cr. fortunei grew sparse, and slower than Cun. lanceolata. Principal component analysis indicated that the soil properties in the Cr. fortunei plantation were better than those in the Cun. lanceolata plantation. Redundancy analysis revealed that the differences in stand structure were affected significantly by the total nitrogen content and pH value. Thus, we propose that Cr. fortunei is planted for priority consideration in the subtropical high mountain area. Further, tending operations and application of fertilizers need to be conducted in Cun. lanceolata forests. Based on theoretical and experimental data, the present study explains why local farmers are more willing to plant Cr. fortunei, and thus provides a useful theoretical reference for the development of sustainable plantations in the future.     

Traditional farming in the mountainous region of Bangladesh and its modifications

Shifting cultivation is a traditional farming system practiced in the tropical mountainous areas. Although it has been widely perceived as an economically inefficient and environmentally harmful agricultural production system, recent science reviews, however, indicate that the deleterious impacts of shifting cultivation on environment may have been overestimated. Despite the pressures of agricultural intensification in areas where shifting cultivation occurs, farmers across the tropics still maintain this traditional farming system. The objective of this study was to explore existing traditional shifting cultivation practices and their various modifications including the innovative farming techniques developed by farmers in the Chittagong Hill Tracts (CHTs), the mountainous region of Bangladesh, and examine their importance with respect to recent socio-economic and environmental changes. The study revealed that shifting cultivation still exists as the most dominant farming method which supports livelihoods and culture of the hill ethnic people. However, demand for more food and household income to meet livelihood needs of an increasing population combined with a rapid deterioration of soil and water quality over decades contributed to development of innovative farming practices through fallow land farming, crop substitution, agroforestry and homestead gardening in the CHTs. Through these farming techniques farmers maintain a strong relationship with traditional knowledge system embedded in the age-old shifting cultivation practices. Today state policies and market forces act in favor of replacement of traditional farming with intensive cash crop agriculture. It seems that disappearance of traditional farming practices from the hills may threaten local biodiversity and food security. It may be recommended that shifting cultivation should be encouraged in areas where they have potential for contributing to preservation of native biodiversity and ecosystem services, and protection of local peoples’ food security and cultural identity.     

Variation characteristics of temperature and moisture content of seasonal frozen soil during freezing process in Changchun,China

In China, seasonal frozen soil is widely distributed. The freezing damage of subgrade soil in Jilin Province has been one of major engineering geological problems troubling the safety of road, in which some common damage phenomena, such as frost heave, subsidence deformation and frost boiling, are all caused by water translocation. Aiming at the phenomenon, the changes of moisture content of seasonal frozen soil in Changchun City are mainly studied by long-term field observation and indoor testing of physical properties under different conditions, and then the variation characteristics of moisture content in soil under different compactness and temperature conditions are realized. The results show that the increasing section of moisture content and negative temperature section all lie in O. 0 - 1.0 m of subgrade. Both lowest air and earth temperature occur in January to February, and the most negative temperature ranges from -7℃ to -10℃.     

Variation characteristics of temperature and moisture content of seasonal frozen soil during freezing process in Changchun, China

In China, seasonal frozen soil is widely distributed. The freezing damage of subgrade soil in Jilin Province has been one of major engineering geological problems troubling the safety of road, in which some common damage phenomena, such as frost heave, subsidence deformation and frost boiling, are all caused bywater translocation. Aiming at the phenomenon, the changes of moisture content of seasonal frozen soil in Changchun City are mainly studied by long-term field observation and indoor testing of physical properties under different conditions, and then the variation characteristics of moisture content in soil under different compactness and temperature conditions are realized. The results show that the increasing section of moisture content and negative temperature section all lie in 0.0-1.0 m of subgrade. Both lowest air and earth temperature occur in January to February, and the most negative temperature ranges from -7 ℃ to -10 ℃.     

Diurnal and seasonal variation of the elevation gradient of air temperature in the northern flank of the western Qinling Mountain range,China

The typically sparse or lacking distribution of meteorological stations in mountainous areas inadequately resolves temperature elevation variability. This study presented the diurnal and seasonal variations of the elevation gradient of air temperature in the northern flank of the western Qinling Mountain range,which has not been thoroughly evaluated. The measurements were conducted at 9 different elevations between 1710 and 2500 m from August 2014 to August 2015 with HOBO Data loggers. The results showed that the annual temperature lapse rates(TLRs) for Tmean,Tmin and Tmax were 0.45?C/100 m,0.44?C/100 m and 0.40?C/100 m,respectively,which are substantially smaller than the often used value of 0.60°C/100 m to 0.65°C/100 m. The TLRs showed no obvious seasonal variations,except for the maximum temperature lapse rate,which was steeper in winter and shallower in spring. Additionally,the TLRs showed significant diurnal variations,with the steepest TLR in forenoon and the shallowest in early morning or late-afternoon,and the TLRs changed more severely during the daytime than night time. The accumulated temperature above 0°C,5°C and 10°C(AT0,AT5 and AT10) decreased at a lapse rate of 112.8?C days/100 m,104.5?C days/100 m and 137.0?C days/100 m,respectively. The monthly and annual mean diurnal range of temperatures(MDRT and ADRT) demonstrated unimodal curves along the elevation gradients,while the annual range of temperature(ART) showed no significant elevation differences. Our results strongly suggest that the extrapolated regional TLR may not be a good representative for an individual mountainside,in particular,where there are only sparse meteorological stations at high elevations.     

Environmental Drivers of Temporal and Spatial Fluctuations of Mesozooplankton Community in Daya Bay,Northern South China Sea

The response of zooplankton to the ecological environment in Daya Bay is unclear under the influence of both climate changes and anthropogenic activities on a seasonal to inter-annual scale. Based on monthly surveys and historical data, we found the zooplankton community had changed temporally and spatially. A total of 134 species was recorded during the study, and copepods dominated numerically in terms of diversity and abundance. Both copepods and cladocerans were the main contributors to zooplankton abundance. The community structure of zooplankton was temporally classified into the warm and cold groups, and spatially into the three groups located in the marine cage-culture area(MCCA), the outflow of nuclear power plants(ONPP) and unpolluted waters(UPW). The zooplankton was characterized by low biomass(dry weight), high diversity and abundance in the warm period in contrast to that in the cold period. Compared with the other two groups, the MCCA group of zooplankton showed high abundance, low diversity and biomass. Variations in dominant species were closely related to temperature, salinity and chlorophyll a concentration.Species diversity and dry weight decreased in comparison with 30 years ago, while zooplankton abundance increased. The seasonal variation in zooplankton was affected mainly by temperature that was controlled by monsoon, while the spatial difference in the community structure was probably due to eutrophication in the MCCA and thermal water discharge from ONPP. The zooplankton community is undergoing great changes with the tendency of miniaturization and gelatinization in recent 30 years in Daya Bay.