The relation of the average sound intensity to range in homogeneous shallow-water

Using a “three parameters” bottom model, the average sound intensity in homogeneous shallow water with constant depth is found by a better expression to be finite for normal mode number of water layer. The space structure of the sound field and some factors controlling the near, middle and far fields are discussed with this expression. This paper was published in Chinese inOceanologia et Limnologia Sinica 14 (2): 198–202, 1983.     

Matched-field inversion of sound speed profile in shallow water using a parallel genetic algorithm

A sound speed profile plays an important role in shallow water sound propagation. Concurrent with in-situ measurements, many inversion methods, such as matched-field inversion, have been put forward to invert the sound speed profile from acoustic signals. However, the time cost of matched-field inversion may be very high in replica field calculations. We studied the feasibility and robustness of an acoustic tomography scheme with matched-field processing in shallow water, and described the sound speed profile by empirical orthogonal functions. We analyzed the acoustic signals from a vertical line array in ASIAEX2001 in the East China Sea to invert sound speed profiles with estimated empirical orthogonal functions and a parallel genetic algorithm to speed up the inversion. The results show that the inverted sound speed profiles are in good agreement with conductivity-temperature-depth measurements. Moreover, a posteriori probability analysis is carried out to verify the inversion results.     

Discussion of some conceptions of a water mass based on the theory of fuzzy sets

The fundamental principle for differentiating water masses is a strict consideration of their “relative interier homogeneity” and obvious exterior differences with others in characteristics. The conceptions of water type, water mass and water system are dealt with on the basis of the theory of fuzzy sets. A proposal to apply the theory of fuzzy sets to define the water mass and its core, independent area, boundary and mixing area is put forward. As an example, the membership function of the surface water masses in the Yellow Sea and East China Sea in August, 1979, are considered. Their cores, independent areas, boundaries, mixing areas and the approximation degrees between different water masses are calculated respectively. The water masses are ranged according to their fuzzy degrees. This paper was published inOceanologia et Limnologia Sinica, 1986,17(2): 102–110. This study was financially supported by National Natural Sciences Foundation of China.     

Relationship between heavy metal pollution and water productivity in Xiamen estuarine harbor

This paper is based on the results of simulation experiments and annual observations on the effect of Cr, Cu, Hg, and Zn on diatom (Chaetoceros sp.Skeletonema costatum andMelosira sulcata) growth. Laboratory experiments reveal many physico-chemical parameters play an important role in the toxicity and accumulation of metals in organisms. The mesotrophic level in the water environment is related to phytoplankton growth. A higher nutrient level can have a negative impact on diatom production. Several of the above metals coexist in the investigated region and exert mainly a negative effect on growth. This paper was published in Chinese in theOceanologia et Limnologia Sinica 17(3):173–183, 1986.     

Influences of wind on vertical structures of temperature and salinity,and upwelling off Zhejiang coast in summer

In this paper the influences of short-term wind on vertical structures of temperature and salinity, and the upwelling off the Zhejiang coast are studied on the basis of hydrographic data and wind near the coast in Summer (1959–1979). It is pointed out that the variations of vertical distributions of temperature and salinity, and upwelling in this region are closely related to short-term (several days to a week) variation of wind observed. The occurrence of double thermocline, a special oceanographic phenomenon, is partially related to unstable SSW wind field. SSW and NE winds in June are two dynamic factors making intermediate warm water form or disappear. Contribution No. 792 from the Institute of Oceanology, Academia Sinica. This paper was published in Chinese inOceanologia et Limnologia Sinica 14 (1): 14–21, 1983.     

The fronts of the Huanghai cold water mass (HCWM) induced by tidal mixing

Since Simpson and Hunter proposed in 1971 the idea that shallow water fronts were induced by tidal mixing, many authors have investigated the tide induced shallow water fronts in various areas. It was generally assumed that a critical value of the stratified parameterK=log₁₀(H/U ³) may be used to identify the location of these fronts. Here, H means the depth of sea waler. U denotes the characteristic velocity of tidal current. and the critical value ofK is generally laken as 1.8–2.0. In this paper. Simpson-Hunter’s stratified parameterK was calculated by using quasi-maximum current velocity (which consists of the six main tidal constituents. i.e.M ₂.S ₂,O ₁.K ₁.M ₄,MS₄) in the H ranghai Sea (Yellow Sea) and in the northern Donghai Sea (East China Sea) as well. Caleulated results show that the areas of the Subei (the northern part of Jiangsu) Shoal. the area off the mouth area of the Changjiang (the Yangtze) River, along the coast of Shandong Peninsula. and along the western coast of Korea, the tidal mixing is strong. Calculations also show that along the whole boundary of the HCWM and also along the western boundary of the cyclonic eddy in the northern Donghai Sea, i.e. along the fronts, the value ofK is found to be very close to the eritical value 1.8. It is also to be noied that at these locations, the temperature of the surface water is very low. These clearly show that shallow water fronts in the Huanghai Sea and in the northern Donghai Sea as well, are induced by tidal mixing. Contribution No. 1241 from Institute of Oceanology, Academia Sinica. This paper was published in Chinese in theOcean. et Limn. Sinica 16(6): 451–459, 1985.     

Ventilation of the North Pacific Ocean: Water exchange between subtropical and tropical gyres

INTRODUCTIONIntheearly 1 980 s,ventilationtheoryemergedtopredictthehorizontalandverticalstructureofthethermocline.Thereweretwodifferentviewsonthestratifiedoceans.Thefirsttheory (RhinesandYoung,1 982 ,hereafterRY ;YoungandRhines,1 982 )proposedanunventilatedmodelinwhichonlytheuppermostlayerisexposedtothesurfaceandisforcedbythewindstress.Thesecondtheory (Luytenetal.,1 983 ,hereafterLPS)offeredaventilatedmodelwhichisclosertotheclassicalthermoclinetheoriesthanthatofthefirstone (e.g .,Nee…     

Studies on the eggs and larvae,ofSardinella aurita (Cuv. & Val.) in the fishing grounds of Southern Fujian and the Taiwan Bank

In this paper, the morphological characters of eggs and larvae ofSardinella aurita (Cuvier & Valenciennes), its spawning ground, spawning seasons and spawning conditions have been studied. Sardinella aurita likes to breed in the upwelling area and selects the Waixie fishing ground as its main spawning ground. The months from February to September are its spawning seasons, reaching its peak in April. In the main spawning ground, the temperature of the surface layer was found to be 24.4–25.2°C, the salinity 33.87–34.07%. and the depth of water between 34–60m. The distribution of the larvae is closely related to the distribution of plankton, the path of migration of adult fish, and the current direction of the water system. In order to protect fishery resources, it is necessary to prohibit catching the spawning fish in the Waixie fishing ground in April, and the catching of immatures from March to June. This paper was published in Chinese inOcean. Limn. Sinica 14(3): 240–248, 1983.     

Proteocephalus sinocyclocheili n. sp. (Cestoda: Proteocephalidae) in a fishSinocyclocheilus grahami tingi Fang from Yunnan, China

A new species ofProteocephalus from the fishSinocyclocheilus grahami tingi, a subspecies endemic to Fuxian Lake in Southwest China,is described. This is the first report of cestode from this host. The new species differs fromP. exiguus, P. Longicollis, P. parasiluri andP. torulosus, the only other species ofProteocephalus currently reported from China, by lacking an apical sucker, fromP. exiguus by having 45–108 rather than 24–54 testes per proglottis, and a maximum of 15 rather than 28 uterine diverticula; fromP.longicollis by having an average of 45–108 rather than 75–115 testes per proglottis and testes being in a single layer instead of 2 layers; and fromP. torulosus by having 45–108 instead of 150 testes per proglottis and testes being in 3–4 layers; and fromP. parasiluri by having 45–108 rather than 180 testes per proglottis. It resemblesP. percae, P. pollanicola, andP. thymalli in number of testes and uterine diverticula, but differs by lacking an apical sucker.Proteocephalus thymalli, like the new species, has a vagina that opens dorsally to the cirrus sac, butP. thymalli slao has postovarian testes, which the new species lacks. The possible significance of parasitological examinations of the other two subspecies ofS. grahami in two lakes close to Fuxian Lake is discussed in relation to host speciation and coevolution of the host-parasite system.     

Distribution of methyl mercury in <Emphasis Type="Italic">Rana chensinensis</Emphasis> and environmental media in gold-mining areas of upper reaches of Songhua River,China

The distribution characteristics of methyl mercury in Rana chensinensis and water, sediment and soil in gold-mining areas of the upper reaches of the Songhua River, China were studied by field sampling and laboratory testing. The results show that the methyl mercury contents in water, sediment and soil in gold-mining areas are much higher than those in the control site, indicating that gold-mining activities intensify the methyl mercury pollution in the study area. Methyl mercury contents are in a descending order of sediment > soil > water in the environment, and in a descending order of brain > viscera > muscle > skin in Rana chensinensis. There are significant correlation between methyl mercury contents in Rana chensinensis and those in water and sediment. In particular, the methyl mercury content in the skin of Rana chensinensis is positively correlated with those in water and sediment in spring. Therefore, skin is one of main intake pathways for methyl mercury due to its high permeability.     

A transformation model forLaminaria Japonica (Phaeophyta,Laminariales)

A genetic transformation model for the seaweedLaminaria japonica mainly includes the following aspects:

1. 1.

   The method to introduce foreign genes into the kelp,L. japonica

   Biolistic bombardment has been proved to be an effective method to bombard foreign DNA through cell walls into intact cells of both sporophytes and gametophytes. The expression ofcat andlacZ was detected in regenerated sporophytes, which suggests that this method could induce random integration of foreign genes.

   Promoters to drive gene expression

2. 2.

   The CaMV35S promoter was first used by us to induce the expression of GUS gene in brown algae. But results of further studies suggested that CaMV35S could be a tissue-specific promoter. Our use of SV40 promoter resulted in both transient and stable expression oflacZ andcat in sporophytes or gametophytes. No GUS or LacZ background was found in either sporophytes or gametophytes.The regeneration route of transgenic kelp

   The regeneration efficiency of explants is still very low. By using female gametophytes as gene hosts and parthenogenesis as regeneration route, CAT activity and LacZ activity were detected in regenerated sporophytes of parthenogenetic kelp. li]4.|The way to select transgenic kelp

3. 1.

   Results of sensitivity tests showed that kelp was only sensitive to chloramphenicol and hygromycin among many antibiotics. The regenerated sporophytes by parthenogenesis were more sensitive to hygromycin than to chloramphenicol. Resistant kelp was created by transforming female gametophytes with pSV40-CAT and stimulating parthenogenesis followed by selection in medium with lethal concentration of chloramphenicol.

   Safety consideration of transgenic kelp

   L. japonica was originally introduced from Japan. In China it is a cultured population. The possibility of its negative impact on natural populations is very low. 2) The vectors and target genes used for transformation should be restricted in order to avoid any negative impacts on human health and environment. 3) Specially devised containers (3.6 L, made of 200 μm membrane) were used to ensure that the kelp cannot escape or be eaten by marine animals. 4) To avoid the release of spores, it is very necessary to harvest the kelp at suitable age before the sporangium forms.

     

CYTOLOGICAL COMPARISON OF GAMETOGENESIS OF SCALLOPS, ARGOPECTEN IRRADIANS AND CHLAMYS FARRERI

Histological characteristics of gametogenesis of two kinds of scallops, gonochoric Chinese scallop, Chlamys farreri and hermaphroditic bay scallop, Argopecten irradians were investigated in this study. Spermatogenesis in C. farreri has different developmental stages: spermatogonia, primary spermatocyte, second spermatocyte, spermatid and spermatozoon. A large number of same developmental stage spermatic cells converge at a definite area of the testis. Premeiotic, previtellogenic and vitellogenic oocytes can be found during oogenesis in C. farreri, where oocyte distribution is obviously irregular. The A. irradians gonad consists of two different parts in one individual: one part functions as testis, the other as ovary. Between these two parts is a special appearance area, where a large number of spermatic cells are bound with two layers of acellular substance with many oocytes in it.     

Dynamic model for oil slick dispersion into a water column—A wind-driven wave tank experiment

A dynamic experiment for oil dispersion into a water column was performed with a 21 m long, 0.5 m wide, and 1 m high wind-driven wave tank. At wind velocity between 6–12 m/s and with the oil slick kept constant (about 1 μm), the rate of the oil content increase in the water column could be approximated from the difference between the dispersion rate (R) of the oil slick and the coagulation rate (R′) of the dispersed oil slick. Assuming the coagulation rate is directly proportional to the concentration of the water dispersed oil slick (i. e.R′-K ^*C), the integral form of the dynamic model can be expressed asC=R ^* [1−exp(−K ^*t)]/K and parametersR andK can be regressed with a computer. The relative deviation of model results from the experimental data was mainly less than 10%. The oil slick dispersion rate (R) had exponential relationship with the wind velocity (U), and can be fitted with a formulaR=A ^* (U+1) ^B . The fitted constant of the coagulation rate,K(0.8–3.0^* 10⁻³ min⁻¹) did not have significant relationship with the wind velocity.     

Spatio-temporal variability of small fishes related with environmental factors in a typical domestic tap water lake,Eastern China

The knowledge of prey small fish stock, distribution and abundance is necessary to guide stocking of piscivorous fish for the biomanipulation in domestic tap water lakes. This study describes the current status of small fish community in Lake Kuilei (China), and examines the spatial and seasonal variations of the community in relation to key environmental factors. Based on submerged macrophyte cover and water depth, the lake was divided into five major habitats: (1) macrophyte covered shallow habitat of water depth < 2.00 m, (2) uncovered or less-covered shallow habitat (2.00 m–3.50 m), (3) uncovered medium shallow habitat (3.50 m–5.00 m), (4) uncovered medium deep habitat (5.00 m–6.50 m) and (5) uncovered deep habitat (6.50 m–8.50 m). The abundance and composition of small fish were monitored by benthic fykenet sampling from April 2013 to January 2014. A total of 2881 individuals belonging to 5 families and 21 species were collected. Based on their abundance (accounted for 88.96% of the total) and occurrence (more than 33.33%), Acheilognathus chankaensis, Acheilognathus macropterus, Microphysogobio microstomus, Pseudorasbora parva and Rhinogobius giurinus were recognized as dominant small fish species. The results of correlation analysis identified that species richness ( Sr ), Shannon-Wiener diversity index ( H′ ) and Margalef′s richness index ( D ) were significantly negatively correlated with water depth, but positively correlated with biomass of submerged macrophytes.Redundancy analysis (RDA) revealed that the spatial distributions of most small fishes were negatively associated with water depth. The details of these findings are beneficial to understanding the adaptation of the small fishes in degraded environments, and to developing suitable biomanipulation strategies for the management of fish resources and water quality in the lakes along the lower reach of the Changjiang (Yangtze) River basin.

     

Impact of redox-stratification on the diversity and distribution of bacterial communities in sandy reef sediments in a microcosm

Relationships between microbial communities and geochemical environments are important in marine microbial ecology and biogeochemistry. Although biogeochemical redox stratification has been well documented in marine sediments, its impact on microbial communities remains largely unknown. In this study, we applied denaturing gradient gel electrophoresis (DGGE) and clone library construction to investigate the diversity and stratification of bacterial communities in redox-stratified sandy reef sediments in a microcosm. A total of 88 Operational Taxonomic Units (OTU) were identified from 16S rRNA clone libraries constructed from sandy reef sediments in a laboratory microcosm. They were members of nine phyla and three candidate divisions, including Proteobacteria (Alpha-, Beta-, Gamma-, Delta-, and Epsilonproteobacteria), Actinobacteria, Acidobacteria, Bacteroidetes, Chloroflexi, Cyanobacteria, Firmicutes, Verrucomicrobia, Spirochaetes, and the candidate divisions WS3, SO31 and AO19. The vast majority of these phylotypes are related to clone sequences from other marine sediments, but OTUs of Epsilonproteobacteria and WS3 are reported for the first time from permeable marine sediments. Several other OTUs are potential new bacterial phylotypes because of their low similarity with reference sequences. Results from the 16S rRNA, gene clone sequence analyses suggested that bacterial communities exhibit clear stratification across large redox gradients in these sediments, with the highest diversity found in the anoxic layer (15–25 mm) and the least diversity in the suboxic layer (3–5 mm). Analysis of the nosZ, and amoA gene libraries also indicated the stratification of denitrifiers and nitrifiers, with their highest diversity being in the anoxic and oxic sediment layers, respectively. These results indicated that redox-stratification can affect the distribution of bacterial communities in sandy reef sediments.     

Distributions of inorganic nutrients in the bohai sea of china

1　Introduction　TheBohaiSeaislocatedinthenorthernChinawithlongitudesofbetween 117°38′Eand 12 2°31′Eandlat itudesofbetween 37°0 8′Nand 4 1°0 2′N .Itisashal lowseawithanaveragewaterdepthof 18m (LiuandZhang ,2 0 0 0 ) .Severalbigrivers ,suchastheLiaoheRiver,theHaiheRiverandtheYellowRiver ,findtheirwaysintotheBohaiSeaandtransportlargeamountofnutrientsandsuspendedmattersfromthecontinentintothesea (Zhangetal.,1994 ;Zhang ,1996 ) .Duringthelasttwodecades ,marineenviron mentintheBohai…     

Extraction and PTP1B inhibitory activity of bromophenols from the marine red alga Symphyocladia latiuscula

Previously, we had characterized several structurally interesting brominated phenols from the marine red alga Symphyocladia latiuscula collected from various sites. However, Phytochemical investigations on this species collected from the Weihai coastline of Shandong Province remains blank. Therefore, we characterized the chemical constituents of individuals of this species collected from the region. Eight bromophenols were isolated and identified. Using detailed spectroscopic techniques and comparisons with published data, these compounds were identified as 2,3-dibromo-4,5-dihydroxybenzyl methyl ether (1), 3,5-dibromo-4-hydroxybenzoic acid (2), 2,3,6-tribromo-4,5-dihydroxymethylbenzene (3), 2,3,6-tribromo-4,5-dihydroxybenzaldehyde (4), 2,3,6-tribromo-4,5-dihydroxybenzyl methyl ether (5), bis(2,3,6-tribromo-4,5-dihydroxyphenyl)methane (6), 1,2-bis(2,3,6-tribromo-4,5-dihydroxyphenyl)-ethane (7), and 1-(2,3,6-tribromo-4,5-dihydroxybenzyl)-pyrrolidin-2-one (8). Among these compounds, 1 and 2 were isolated for the first time from S. latiuscula. Each compound was evaluated on the ability to inhibit protein tyrosine phosphatase 1B (PTP1B), which is a potential therapeutic target in the treatment of type 2 diabetes. Bromophenols 5, 6, and 7 showed strong activities with IC50 values of 3.9, 4.3, and 3.5 μmol/L, respectively. This study provides further evidence that bromophenols are predominant among the chemical constituents of Symphyocladia, and that some of these compounds may be candidates for the development of anti-diabetes drugs.     

Retrieval of chlorophyll a and suspended solid concentrations by hyperspectral remote sensing in Taihu Lake, China

Chlorophyll α（ch1-α） and suspended solid concentrations are two frequently used water quality parameters for monitoring a lake. Traditional measurement of ch1-α and suspended solids, requiring laborious laboratory work, which is often expensive and time consuming. Hyperspectral remote-sensing measurement provides a fast and easy tool for estimating water trophic status. In situ hyperspectral data on March 7-8, July 6-7, September 20 and December 7-8, 2004 and the corresponding water chemical data were used to regress the algorithm of water quality parameters. Results showed that the peak of water leaving radiance around 700 nm （R700） varied proportionally with ch1-α concentration, and moved to infrared when algal bloom occurred. The reflectance ratio of R702/R685 was well correlated with ch1-α when water surface in no algal bloom case and the correlation coefficient was better if absorption of phycocyanin was considered. The reflectance ratio R620/R531 was highly correlated to the concentration of suspended solids. The relationship between suspended solids and other band groups were also compared. Secchi disk depth could be calculated by non-linear correlation with suspended solids concentration.     

Diurnal variation in relative photosynthetic performance of marestail (Hippuris vulgaris Linn.) Across a water temperature gradient using PAM fluorometry in Jiuzhaigou National Nature Reserve, Sichuan Province, China

This paper reports the first photosynthetic study of marestail in Jiuzhaigou. In this work, we used PAM fluorometry to examine photosynthetic rates of submerged and emerged marestail in three lakes. Three lakes were studied across a gradient of water temperature, with low water temperature conditions in Grass Lake and Arrow Bamboo Lake, and higher water temperature in Five Colored Lake. In the field, electron transport rates (ETR_max) were measured as rapid light curves (RLCs) by in situ yield measurements. Submerged and emerged marestail showed higher photosynthetic activity in Five Colored Lake compared to the other lakes, a response consistent with the adaptation of marestail in Five Colored Lake to high water temperature. The optimal temperature for photosynthesis of submerged marestail in Jiuzhaigou was about 12 °C. Nonphotochemical quenching (NPQ) of submerged and emerged marestail increased with increasing water temperature. Maximum quantum yield (F _v/F _m) of submerged marestail in Five Colored Lake showed full recovery at 1700 h due to higher NPQ. Further, the chlorophyll a for submerged marestail was the highest in Grass Lake and the lowest in Five Colored Lake. These results indicate that in different lakes the function of these aquatic plants is associated with a diversity of place-dependent environmental conditions, especially water temperature that leads to pronounced differences in the plant’s ecophysiological reactions.     

Tidal Analysis of High and Low Water Data

1　Introduction　Itisverycommontousetidalconstantsinoceanen gineering .Ingeneral,tidalconstantscanbeobtainedfromtidalharmonicanalysisbasedonthehourlydata .However ,thereisamassofhistoricaltidaldatamere lyonthetimeandmagnitudesofsuccessivehighandlowwaterlevels .Moreover ,sometimeswemayhavetidaltableswhichonly givethepredictedvaluesofhighandlowwatersforsomeports,withoutinforma tionontidalconstants .Inthesetablesthetimeinter valsbetweensuccessivetidalextremaareirregular .Therefore ,mostofthestan…