极大螺旋藻铁型超氧物歧化酶的纯化及性质

极大螺旋藻 Ｓｐｉｒｕｌｉｎａｍａｘｉｍａ藻体ＳＯＤ聚丙烯酰胺梯度凝胶电泳呈现４条同工 酶带, Ｈ_ ２Ｏ_２明显抑制该酶活性, ＫＣＮ对酶活性无影响,确认为 Ｆｅ－ＳＯＤ经硫酸铵分部盐析、离子交 换柱层析及凝胶过滤,纯化到电泳单斑点均－程度．纯化的Ｆｅ－ＳＯＤ分子量为３９．３ＫＤ,亚基分子 量为２０ＫＤ金属元素分析表明,每个亚基含０ ５５个Ｆｅ原子,该酶在紫外区最大吸收峰值为 ２７５．８nｍ、该酶氨基酸组成与蓝、绿藻和高等植物的Ｆｅ－ＳＯＤ相似,但它含有较高的丙氨酸,酸性氨 基酸和碱性氨基酸比值与低等植物及原核生物相近而明显高于高等植物．     

百草枯和H2O2预处理提高盐泽螺旋藻对铅的耐受性

用１０μｍｏｌ．Ｌ＾－１的百草枯（Ｐａｒａｑｕａｔ）和２ｍｍｏｌ．Ｌ＾－１的Ｈ２Ｏ２对盐泽螺旋藻（Ｓｐｉｒｕｌｉｎａ ｓｕｂｓａｌｓａ）作预处理,以诱导提高藻细胞内超氧物歧化酶（ＳＯＤ）,过氧化氢酶（ＣＡＴ）和过氧化物酶（ＰＯＤ）的活性,随后用４０ｍｇ．Ｌ＾－１的Ｐｂ＾２＋进行肋迫试验,实验结果表明,预处理组的藻细胞生长量和光合速率的下降幅度明显较未预处理组小;预处理组的ＳＯＤ、ＣＡＴ活性虽有衰减     

含Fe~(3 )的硅酸盐尖晶石γ-Fe_2SiO_4的Mssbauer效应研究

对３个合成的含Ｆｅ~３＋的铁硅酸盐尖晶石γ－Ｆｅ_２ＳｉＯ_４进行了电子探针分析,并在２９８,１５０和９５Ｋ的温度下对它们进行了Ｍｏｓｓｂａｕｅｒ谱的测试．每个温度下的Ｍｏｓｓｂａｕｅｒ谱均由两个双峰组成．这两个双峰分别被指派给八面体位置上的Ｆｅ~２＋和四面体位置上的Ｆｅ~３＋,并且根据Ｍｏｓｓｂａｕｅｒ吸收面积给出了占位结果表明,Ｆｅ~３＋和少量的Ｓｉ~４＋分别占据在四面体和八面体位置上．根据Ｍｏｓｓｂａｕｅｒ分析所得到的占位,使用经过修正的公式,计算了八面体和四面体位置的平均键长．在此基础上,八面体和四面体的平均键长进而被用来计算含Ｆｅ~３＋的γ－Ｆｅ_２ＳｉＯ_４的晶胞参数和氧参数．此外,使用次近邻效应解释了含Ｆｅ~３＋的γ－Ｆｅ_２ＳｉＯ_４Ｍｏｓｓｂａｕｅｒ谱的Ｆｅ~３＋谱线的宽化现象。     

RECENT UNDERTHRUSTS OF TIANSHAN AND POSSIBLE MECHANISM OF THEIR ORIGIN

ＲＥＣＥＮＴＵＮＤＥＲＴＨＲＵＳＴＳＯＦＴＩＡＮＳＨＡＮＡＮＤＰＯＳＳＩＢＬＥＭＥＣＨＡＮＩＳＭＯＦＴＨＥＩＲＯＲＩＧＩＮ￥Ｏ．Ｋ．Ｃｈｅｄｉａ（ＩｎｓｔｉｔｕｔｅｏｆＳｅｉｓｍｏｌｏｇｙＡｃａｄｅｍｙｏｆＳｃｉｅｎｃｅｓＲｅｐｕｂｌｉｃｏｆＫｉｒｇ...     

SEDIMENTATION IN LOW HEAD RIVER RESERVOIRS

ＳＥＤＩＭＥＮＴＡＴＩＯＮＩＮＬＯＷＨＥＡＤＲＩＶＥＲＲＥＳＥＲＶＯＩＲＳ￥ＷｉｌｈｅｌｍＢｅｃｈｔｅｌｅｒ（Ｐｒｏｆ．Ｄｒ．－Ｉｕｇ．ＩｎｓｔｉｔｕｔｅｏｆＨｙｄｒｏｓｃｉｅｎｃｅｓ，ＦｅｄｅｒａｌＡｒｍｅｄＦｏｒｃｅｓＵｎｉｖｅｒｓｉｔｙＭｕｎｉ...     

SOIL LOSS INFLUENCED BY SLOPE LENGTH IN THE HILLY LOESS REGION, CHINA

ＳＯＩＬＬＯＳＳＩＮＦＬＵＥＮＣＥＤＢＹＳＬＯＰＥＬＥＮＧＴＨＩＮＴＨＥＨＩＬＬＹＬＯＥＳＳＲＥＧＩＯＮ，ＣＨＩＮＡＣＡＩＱｉａｎｇｇｕｏ１ａｎｄＳｈｉＵ－ＨｕｎｇＬＵＫ２Ｉ．ＩＮＴＲＯＤＵＣＴＩＯＮＳｌｏｐｅｌｅｎｇｔｈｈａｓｌｏｎｇｂｅｅｎｒｅｃ...     

AN EXPERIMENTAL STUDY OF BED DEFORMATION IN UNSTEANY NON-UNIFORM FLOWS

ＡＮＥＸＰＥＲＩＭＥＮＴＡＬＳＴＵＤＹＯＦＢＥＤＤＥＦＯＲＭＡＴＩＯＮＩＮＵＮＳＴＥＡＮＹＮＯＮ－ＵＮＩＦＯＲＭＦＬＯＷＳ￥ＺｈａｏｙｉｎＷａｎｇ；ＷｏｌｆｇａｎｇＫｒｏｎ；ａｎｄＥｒｉｃｈＪ．ＰＩａｔｅ（Ｐｒｏｆ．Ｄｒ．ＳｅｎｉｏｒＥｎｇｉｎｅｅｒ...     

MATHEMATICAL MODEL OF OVERLAND FLOW AND MECHANISM OF SOIL CONSERVATION FOR FORESTED STEEP HILLSLOPE－－（II）Mechanism of Soil Conservation Over Steep Hillslope

ＭＡＴＨＥＭＡＴＩＣＡＬＭＯＤＥＬＯＦＯＶＥＲＬＡＮＤＦＬＯＷＡＮＤＭＥＣＨＡＮＩＳＭＯＦＳＯＩＬＣＯＮＳＥＲＶＡＴＩＯＮＦＯＲＦＯＲＥＳＴＥＤＳＴＥＥＰＨＩＬＬＳＬＯＰＥ－－（ＩＩ）ＭｅｃｈａｎｉｓｍｏｆＳｏｉｌＣｏｎｓｅｒｖａｔｉｏｎＯｖｅｒＳｔ...     

STUDY ON THE MATHEMATICAL MODEL OF HEAVY－METAL POLLUTANT TRANSPOPT－TRANSFORMATION IN RIVERS

ＳＴＵＤＹＯＮＴＨＥＭＡＴＨＥＭＡＴＩＣＡＬＭＯＤＥＬＯＦＨＥＡＶＹ－ＭＥＴＡＬＰＯＬＬＵＴＡＮＴＴＲＡＮＳＰＯＰＴ－ＴＲＡＮＳＦＯＲＭＡＴＩＯＮＩＮＲＩＶＥＲＳ￥Ｓｕｉ－ｌｉａｎｇＨｕａｎｇａｎｄＺｈａｏ－ｈｕｉＷａｎ（ＰｏｓｔｄｏｃｔｏｒａｌＦｅ...     

Body waveform inversion for the source process of the February 3,1996 Lijiang,Yunnan earthquake

ＢｏｄｙｗａｖｅｆｏｒｍｉｎｖｅｒｓｉｏｎｆｏｒｔｈｅｓｏｕｒｃｅｐｒｏｃｅｓｏｆｔｈｅＦｅｂｒｕａｒｙ３,１９９６Ｌｉｊｉａｎｇ,ＹｕｎｎａｎｅａｒｔｈｑｕａｋｅＹＡＮＧＸＵ１）（徐扬）ＭＡＳＡＹＵＫＩＫＩＫＵＣＨＩ２）（菊地正幸）ＹＯＵ－ＪＩＮ...     

一秋龄性成熟中华绒螫蟹的生物学──1.外部形态特征及性腺变化

本文描述了一秋龄性腺成熟中华绒螯蟹（简称河蟹）的外部特征并与一秋龄未成熟及二秋龄成熟河蟹进行比较,同时给出了体重与壳宽的回归方程．保安湖的研究结果表明,对于一秋龄雌蟹,体重大于２９．８ｇ（壳宽为４．０３ｃｍ）全部成熟,而体重小于１３．１ｇ（壳宽为３．００ｃｍ）均不成熟,体重在１３、１－２９．８ｇ之间,既有成熟又有未成熟．对于一秋龄雄蟹,体重大于２６．０ｇ（壳宽３．９１ｃｍ）,全部成熟,而于１２．０ｇ壳宽２．６６ｃｍ）均未成熟,体重在１２．０－２６．０ｇ之间,既有成熟者又有未成熟个体．对一秋龄成熟河蟹成熟系数逐月变化的观察表明,在翌年３月份达到峰值,雌蟹为１２． ３２± １． ７５％,雄蟹为 ４． ２４± ０． ０６％;此后,性腺迅速退化,至翌年七月上旬,卵巢退变成细索状结构．己无完整的卵粒．     

Effects of 4-nonylphenol exposure in mussels (Mytilus galloprovincialis) and crabs (Carcinus aestuarii) with particular emphasis on vitellogenin induction

Since it is often difficult to estimate possible adverse effects due to contamination in selected ecosystems, multi-species biomonitoring may provide more information, taking into account different routes of exposure, ecological roles and metabolic capabilities of animals. In this context, we exposed for 7 days the mussel Mytilus galloprovincialis and the crab Carcinus aestuarii to 4-nonylphenol (NP), a well-known xenoestrogen. In mussels (0–0.2 mg NP l⁻¹), we measured NP bioaccumulation in soft tissues and vitellogenin (Vg)-like protein levels in digestive glands from both males and females by the alkali-labile phosphate assay (ALP). As no reference data were available for crab exposure, the NP 96-h LC₅₀ value was previously determined. Then, in sublethally exposed (0–1.0 mg NP l⁻¹) male crabs, NP bioaccumulation and Vg levels were measured in hemolymph, gonads and digestive gland. Bioaccumulation of NP increased from 43 to 371 μg g⁻¹ d.w. in mussels, and from 3.6 to 37 μg g⁻¹ d.w. in crabs, depending on the NP concentration in water. Dose-dependent Vg-like protein induction was observed in both species, appearing to be related to NP bioaccumulation, although a partial decrease was recorded at the highest concentration tested. A similar trend was observed in both digestive gland and gonad of exposed crabs; Vg increased to a lesser extent, although significantly, in hemolymph. Results demonstrated that NP induces Vg synthesis both in male and female mussels, as well as in male crabs. On the basis of the responsiveness of both species investigated, a multi-species approach is indicated in biomonitoring programmes.     

Morphological changes in branchial mitochondria-rich cells of the teleost Paralichthys olivaceus as a potential indicator of CO2 impacts

We studied the morphological and biochemical changes of mitochondria-rich cells (MRCs) of a demersal teleost, Paralichthys olivaceus, during exposure to 0.98, 2.97 and 4.95 kPa pCO₂. The apical opening area of MRCs increased 2.2 and 4.1 times by 24 h exposure to 2.97 and 4.95 kPa pCO₂, respectively, while the cross-sectional area or density of MRCs did not change. Gill Na⁺/K⁺-ATPase activity more than doubled at 72 h and then returned to the pre-exposure level at 168 h in 0.98 kPa pCO₂, while it increased 1.7 times at 24 h at 4.95 kPa. These results indicate that the apical opening area of MRCs and the gill Na⁺/K⁺-ATPase activity may be used as an indicator of acute (up to 72 h), but not chronic, impacts of high (>1 kPa) seawater CO₂ conditions in P. olivacues. Limitations of those parameters as indices of CO₂ impacts are discussed.     

Net, actual, and potential sediment-water interface NH4 fluxes in the northern Gulf of Mexico (NGOMEX): Evidence for NH4 limitation of microbial dynamics

A new approach, combining ¹⁵NH₄⁺ isotope dilution and continuous-flow techniques, provided estimates of “actual” and “net” NH₄⁺ flux and sediment NH₄⁺ demand (SAD) at the sediment-water interface (SWI) of sites in the northern Gulf of Mexico (NGOMEX). The sites included a hypoxic site (C6), two sites with intermediate oxygen levels (B7 and F5), and a normoxic site (CT). Control cores without isotope addition and other cores from the same site treated with ¹⁵NH₄⁺ labeled overflowing water differentiated between net and actual regeneration flux and actual vs. potential uptake flux of NH₄⁺. Experiments were conducted in 2008 before (July) and after (September) two successive hurricanes (Gustav and Ike) and in January and August, 2009. Actual regeneration was significantly higher than net flux at most sites. Net flux did not differ significantly in most sites/dates, but the actual regeneration, and the actual and potential uptake, showed temporal and spatial variation; the flux at the hypoxic site was more active than non-hypoxic sites. SAD, the difference between potential and actual NH₄⁺ uptake flux, was higher at the hypoxic site than at non-hypoxic sites before and after the hurricanes in 2008 and during the hypoxia season in 2009. SAD related negatively to bottom water DO values. Conclusions: (1) net flux often underestimated actual regeneration, (2) hurricane activity decreased N dynamics, and (3) microbial N limitation status at the hypoxic site related to NH₄⁺ removal processes that were independent of oxygen (e.g., anaerobic heterotrophic uptake or anammox). These results indicate a rather consistent NH₄⁺ demand at the SWI during the hypoxic season and suggest that reduced nitrogen may limit microbial dynamics in the region.     

Secular variations ing 1 0 component of geomagnetic field and its origin

The secular variations (SV) ing ₁ ⁰ of geomagnetic field, caused by the interaction between the geomagnetic field and the radial fluid-flow of the outer-core, are discussed with both statistical and analytic methods. When the value ofg ₁ ⁰ s ₂ ⁰ is relatively high compared with other terms, SV ing ₁ ⁰ is characterized by exponential change. When the effect of non-dipole field is notable, SV ing ₁ ⁰ shows complex features. Especially when the value of g ₁ ⁰ is close to zero, SV ing ₁ ⁰ is completely determined by the random process of non-dipole fields. Project supported by the National Natural Science Foundation of China (Grant No. 49429405) and by the Chinese Academy of Sciences.     

利用210Pb、137Cs和241Am计年法测算云南抚仙湖现代沉积速率

通过对云南抚仙湖沉积物柱芯样品的210Pb、137Cs和241Am测试表明,柱芯剖面上有明显的1963年和1986年137Cs蓄积峰,验证了1975年次级蓄积峰存在的可能性,这些峰形完好的蓄积峰对抚仙湖的现代沉积环境有明显的时标意义。利用137Cs计年法得到抚仙湖沉积物自1963年、1975年及1986年以来到2007年的平均沉积速率分别为0.063g/(cm2·a)、0.052g/(cm2·a)和0.039g/(cm2·a),说明了过去近五十年抚仙湖沉积速率整体上经历了一个由快到慢的过程。借助于241Am 的1963年蓄积峰可以提高137Cs计年的准确性。根据210PbCRS计年模式,计算出每个样品深度所对应的年代,与137Cs计年法比较存在一定的偏差,分析了两种计年方法存在差异性的原因。通过质量深度和年代分析,抚仙湖的沉积速率变化幅度比较大,表明抚仙湖近129年来的沉积环境不稳定,可能与相应历史时期的人类活动有密切的关系。     

曝气充氧对城市污染河道内源铵态氮释放的控制

以城市污染河道沉积物和上覆水为研究对象,利用模拟实验方法,探讨不同曝气充氧方式(水曝气EW、底泥曝气ES)对污染河道內源铵态氮(NH4+-N)释放的影响.研究结果发现:从间隙水和沉积物中NH4+-N的削减效果来看,底泥曝气均要优于水曝气;实验结束后,底泥曝气组沉积物与间隙水中NH4+-N含量分别减少63.39%和43.33%,水曝气组分别减少了7.54%和13.98%;从沉积物-水界面NH4+-N的扩散通量变化来看,水曝气组界面通量高于对照组,其变化规律与对照组相似;底泥曝气组沉积物-水界面NH4+-N扩散通量变化过程完全不同于其它两组,在整个试验周期内(除第5 d以外),底泥曝气组的通量低于水曝气组,在第15 d最低,为13.73 mg/(m2.d),仅为水曝气组和对照组的14.68%和19.93%,表明底泥曝气组沉积物NH4+-N的释放潜力低于水曝气组沉积物.     

不同pH处理对菹草(Potamogeton crispus L.)Cr6+毒害效应的影响

对Cr⁶⁺胁迫条件下不同pH处理对菹草无菌苗元素、光合色素、活性氧、丙二醛、抗氧化酶、脯氨酸(Pro)及多胺(PAs)含量的影响进行研究,以探讨不同pH处理影响水生植物Cr⁶⁺毒害效应的机制.结果表明:(1)偏酸性pH处理加剧菹草对铬的蓄积,而pH为7.0时Cr⁶⁺胁迫所造成的矿质元素失衡有所减弱;(2)Cr⁶⁺胁迫下pH为6.0~8.0的处理延缓了菹草的失绿症状,促进了光合色素的合成;(3)Cr⁶⁺胁迫下菹草超氧化物歧化酶、过氧化氢酶的活性在pH为6.0和7.0的处理组诱导程度最小;(4)Cr⁶⁺胁迫下pH为6.0和7.0处理的菹草Pro含量显著提高;(5)pH为4.0和5.0的处理使腐胺(Put)含量显著提高,而pH为6.0和7.0的处理则促进亚精胺(Spd)含量及游离态(Spd+Spm)/Put比值上升,精胺(Spm)含量总体趋势为随pH值升高而逐步上升,仅在pH为4.0和5.0的处理组中低于Cr⁶⁺胁迫对照组.因此,pH条件可影响菹草对铬及营养元素的积累,抗氧化酶、Pro、PAs代谢等生理生化过程,导致铬毒性效应差异.在pH为6.0~7.0的范围内,菹草能调节抗氧化酶系统,较有效地清除体内活性氧,提高机体中Pro、PAs含量及游离态(Spd+Spm)/Put比值,减轻膜系统脂质过氧化,维持机体内矿质元素平衡,致使毒性效应达到最小程度.     

Cr6+胁迫对水花生(Alternanthera philoxeroides)愈伤组织多胺代谢的影响

利用植物组织培养技术培养水花生愈伤组织并以此为实验材料,研究了不同Cr6+浓度(0、0.1、0.3、0.6、0.9 mmol/L)对水花生愈伤组织中腐胺(putrescine,Put)、亚精胺(spermidine,Spd)、精胺(spermine,Spm)、脯氨酸(Pro)含量及精氨酸脱羧酶(ADC)、鸟氨酸脱羧酶(...