Statistical principles for ecological status classification of Water Framework Directive monitoring data

Bias, precision and confidence of the classification framework are crucial elements for decisions to invest large sums to improve the ecological quality. In this study, the statistical principles for classification in relation to WFD are outlined and exemplified. Indicator adjustment to seasonal variation and other significant covariates reduces bias and improves precision. Precision is generally improved using annual means with seasonal adjustment instead of seasonal means. For classification I argue that the balance between costs of monitoring and reduction measures is only fully maintained by the fail-safe approach. The required monitoring efforts to ensure a precise classification are substantially higher than envisaged in WFD, for nutrients and phytoplankton measurements as high as 500 observations to characterise a water body. It must be ensured that sufficient monitoring data become available for classification, while indicator bias and precision is improved through modelling and further development of measurement techniques.     

Using historical data, expert judgement and multivariate analysis in assessing reference conditions and benthic ecological status, according to the European Water Framework Directive

The European Water Framework Directive (WFD) establishes a framework for the protection and improvement of estuarine and coastal waters, trying to achieve 'good surface water status at the latest 15 years after the date of entry into force of this Directive'. One of the biological elements that should be analysed is the benthos and, as such, the WFD normative definitions describe the aspects of the benthic communities that must be included in the ecological status assessment of a water body. Therefore, it is essential to include, in the assessment, the different metrics that address those parameters identified in the normative definitions for each of the ecological status classes. In this contribution the use of the AMBI, richness and diversity, combined with the use, in a further development, of factor analysis together with discriminant analysis, is presented as an objective tool (named here M-AMBI) in assessing ecological quality status. This assessment requires previous classification of water bodies and typologies, together with the definition of reference conditions; this is undertaken in this contribution using historical data, expert judgement and multivariate analysis. The study has been undertaken by examining changes in benthic communities in the Basque Country, over the last decade, as a case-study, to demonstrate the accuracy and potential of these methodologies.     

A new methodology based on littoral community cartography dominated by macroalgae for the implementation of the European Water Framework Directive

Macroalgae is a biological key element for the assessment of the ecological status in coastal waters in the frame of the European Water Framework Directive (WFD, 2000/60/EC). Here we propose a methodology for monitoring water quality based on the cartography of littoral and upper-sublittoral rocky-shore communities (CARLIT, in short). With the use of spatial databases, GIS, and available information about the value of rocky-shore communities as indicators of water quality, it is possible to obtain an environmental quality index representative of the ecological status of rocky coasts. This index, which completely fulfils the requirements of the WFD, is expressed as a ratio between the observed values in the sector of shore that is being assessed and the expected value in a reference condition zone with the same substrate and coastal morphology (Ecological Quality Ratio, EQR). The application of this index to the coast of Catalonia (North-Western Mediterranean) is presented.     

Palaeoecology, reference conditions and classification of ecological status: the EU Water Framework Directive in practice

The European Union's Water Framework Directive (WFD) requires that all Member States within the European Union determine reference conditions for aquatic ecosystems to provide a baseline against which to measure the effects of past and present activities. Reference conditions are subsequently used to classify the ecological status of European waters. The decisions regarding environmental status will be important future elements in the management of European coastal waters. We have developed a number of classification scenarios for total nitrogen (TN) in the overlying waters of the southern part of Roskilde Fjord, Denmark, taking as our basis a palaeoecological reconstruction of fluctuations in TN between 1850 and 1995. We present a provisional classification scheme for the ecological status of Roskilde Fjord, sensu the WFD. Decision(s) regarding the deviation from reference conditions will give a wide range of apparent ecological status from good, through moderate and poor, to bad depending upon the definition of an acceptable deviation from reference conditions. The determination of an acceptable deviation will ultimately be a political decision, and will result in a wide range in the protection of coastal waters in Europe. There is still, however, an urgent need for a sound scientific documentation of the various scenarios for the implementation of the WFD.     

A revisitation of TRIX for trophic status assessment in the light of the European Water Framework Directive: application to Italian coastal waters

The trophic status classification of coastal waters at the European scale requires the availability of harmonised indicators and procedures. The composite trophic status index (TRIX) provides useful metrics for the assessment of the trophic status of coastal waters. It was originally developed for Italian coastal waters and then applied in many European seas (Adriatic, Tyrrhenian, Baltic, Black and Northern seas). The TRIX index does not fulfil the classification procedure suggested by the WFD for two reasons: (a) it is based on an absolute trophic scale without any normalization to type-specific reference conditions; (b) it makes an ex ante aggregation of biological (Chl-a) and physico-chemical (oxygen, nutrients) quality elements, instead of an ex post integration of separate evaluations of biological and subsequent chemical quality elements. A revisitation of the TRIX index in the light of the European Water Framework Directive (WFD, 2000/60/EC) and new TRIX derived tools are presented in this paper. A number of Italian coastal sites were grouped into different types based on a thorough analysis of their hydro-morphological conditions, and type-specific reference sites were selected. Unscaled TRIX values (UNTRIX) for reference and impacted sites have been calculated and two alternative UNTRIX-based classification procedures are discussed. The proposed procedures, to be validated on a broader scale, provide users with simple tools that give an integrated view of nutrient enrichment and its effects on algal biomass (Chl-a) and on oxygen levels. This trophic evaluation along with phytoplankton indicator species and algal blooms contribute to the comprehensive assessment of phytoplankton, one of the biological quality elements in coastal waters.     

Integrating long-term water and sediment pollution data, in assessing chemical status within the European Water Framework Directive

The European Water Framework Directive (WFD) establishes a framework for the protection and improvement of estuarine (transitional) and coastal waters, attempting to achieve good water status by 2015; this includes, within the assessment, biological and chemical elements. The European Commission has proposed a list of priority dangerous substances (including metals such as Cd, Hg, Ni and Pb), with the corresponding list of environmental quality standards (EQS), to assess chemical status, but only for waters. In this contribution, a long-term (1995–2007) dataset of transitional and coastal water and sediment trace elements concentrations, from the Basque Country (northern Spain), has been used to investigate the response of these systems to water treatment programmes. Moreover, the approach proposed in the WFD, for assessing water chemical status (the ‘one out, all out’ approach), is compared with the integration of water and sediment data, into a unique assessment. For this exercise, background levels are used as reference conditions, identifying the boundary between high and good chemical status. EQS are used as the boundary between good and moderate chemical status. This contribution reveals that the first approach can lead to misclassification, with the second approach representing the pattern shown by the long-term data trends. Finally, the management implications, using each approach are discussed.     

Phytoplankton pigments and epifluorescence microscopy as tools for ecological status assessment in coastal and estuarine waters, within the Water Framework Directive

Inverted microscopy is widespread employed for the analysis of phytoplankton composition within water quality monitoring networks. However, the analysis at the lowest taxonomical level is not always required for ecological status assessment. In addition, inverted microscopy can underestimate the small phytoplankton, and not always distinguish photoautotrophic from heterotrophic cells. In this study, as alternative tools, epifluorescence microscopy and High Performance Liquid Chromatography (HPLC) were employed to characterize phytoplankton communities within waters of different trophic condition. Epifluorescence microscopy confirmed its effectiveness to count the small phytoplankton. Furthermore, significant correlations between nutrients of anthropogenic origin and nanoplankton abundances were found. However, this technique resulted very time-consuming. HPLC together with the CHEMTAX program was more appropriate than inverted microscopy, in terms of cost-effectiveness. Also, the main variability patterns observed in the phytoplankton community structure by HPLC coincided with previous findings in the study area. Nevertheless, a rapid screening at the inverted microscope is recommended.     

The French approach to managing water resources in the Mediterranean and the new European Water Framework Directive

The main content of the new European Water Framework Directive is presented. Within its river basin management approach, a special mention of coastal waters status is made. Among the issues at stake are the setting up of river basin management plans, including coastal waters, and water quality assessment system leading to an harmonized definition of quality objectives and their appropriate indicators. The Rhone-Mediterranean-Corsica Water Master Plan, launched in 1996, is considered to be well fitted to this river basin approach and the necessary tools which go with it. It shows up how a river quality assessment system (SEQ) can be adapted to the coastal waters and how it can progressively lead to an efficient set of publishable environmental and performance indicators. Since planning and implementation are devolved to the lowest appropriate level, a close look is then been given at how such a system can work at the local level through different selected case studies on the French Mediterranean coast. In conclusion, some guidelines are drawn up for future initiatives towards integrated coastal area and river basin management.     

A habitat-specific fish-based approach to assess the ecological status of Mediterranean coastal lagoons

A habitat approach was promoted in the framework of ecological status assessment of transitional waters, assuming the importance of habitat heterogeneity to the overall system status. The approach was applied to the use of fish-based multimetric indices by adapting them to seagrass and marsh habitats in the Venice lagoon, Italy, through selection of appropriate metrics and reference conditions. While for marsh habitats, no clear patterns resulted, the index response for seagrass was consistent with the habitat degradation and loss recorded in the lagoon between 2002 and 2005 and with the higher habitat disturbance in southern and central lagoon sub-basins. The assessment of individual habitats is presented as a first step in the process of evaluating the overall condition of a Mediterranean lagoon environment, which should also take account of the diversity of habitats and their availability within the system to properly define an overall index of ecological status.     

Development of a fish-based index to assess the ecological quality of transitional waters: The case of French estuaries

The Water Framework Directive requires the assessment of the ecological status of transitional waters considering the fish component. An original methodology, based on a pressure-impact approach, was established to develop a multimetric fish-based index to characterize the ecological quality of French estuaries. An index of contamination, based on the chemical pollution affecting aquatic systems, was used as a proxy of anthropogenic pressure. The fish metric selection was based on their response to disturbances tested via statistical models (generalized linear models) taking into account sampling strategy and estuarine features. Four metrics, for which discriminating responses to level of pressure were demonstrated, were retained to constitute the estuarine multimetric fish index. This new tool appeared particularly relevant to detect the contamination effects on fish communities in estuaries. It could help managers to take decisions in order to maintain or reach the good status required by the Water Framework Directive for 2015.     

Dissolved oxygen as a physico-chemical supporting element in the Water Framework Directive

For transitional and coastal waters the Water Framework Directive identifies 5 "General chemical and physiochemical elements supporting the biological elements". The five elements are transparency, thermal conditions, oxygenation conditions, salinity and nutrient conditions. "Supporting" in the context of the directive means that the values of the physicochemical quality elements are such as to support a biological community of a certain ecological status, recognising the fact that biological communities are products of their physical and chemical environment. Physicochemical and hydromophological aspects fundamentally determine the type of water body and habitat, and hence the type specific biological community. The directive does not intended that these supporting elements should be used as surrogates for the biological elements in monitoring. The monitoring and assessment of the physical and physicochemical quality elements will support the interpretation, assessment and classification of the results arising from the monitoring of the biological quality elements. This paper considers the challenges involved in the development of oxygen standards for the directive, their relationship to the biological elements and normative conditions of the directive and to regulatory requirements.     

Marine quality assessment by use of benthic species-abundance distributions: a proposed new protocol within the European Union Water Framework Directive

The aim of this study is to develop a new method for classification of marine benthic quality according to the European Union Water Framework Directive. Tolerance values to environmental disturbance were determined in an objective analysis for benthic species along the Swedish west coast by using 4676 samples from 257 stations. Based on a combination of the species tolerance values, abundance and diversity, a benthic quality index (BQI) was calculated for the assessment of environmental status at a particular station. The qualification of BQI was evaluated in relation to known spatial and temporal gradients of disturbance.     

Application of biomarkers for improving risk assessments of chemicals under the Water Framework Directive: a case study

To answer the requirement of the European Commission's Water Framework Directive (WFD) for biological-effects endpoints to classify the ecological health of aquatic ecosystems, we propose the biomarker response index (BRI). The BRI, based on a suite of biomarkers at different levels of biological response at the individual level, provides an integrated relative measure of the general health status of coastal invertebrates. Using the BRI, the health of mussels (Mytilus edulis) from 10 estuaries classified by the Environment Agency of England and Wales under the WFD was compared. Eight sites were healthier than predicted and two showed a similar health status to that of the predicted point-source pollution risk classification. Results indicate that the BRI offers a potential measure of organism health that can be used in monitoring under the WFD as an additional aid to reduce uncertainty in defining risk classification and to provide better evidence of existing impact.