Cold seep communities in the deep eastern Mediterranean Sea: composition, symbiosis and spatial distribution on mud volcanoes

Two mud volcano fields were explored during the French–Dutch MEDINAUT cruise (1998) with the submersible NAUTILE, one south of Crete along the Mediteranean Ridge at about 2000 m depth (Olimpi mud field) and the other south of Turkey between 1700 and 2000 m depth (Anaximander mud field) where high methane concentrations were measured. Chemosynthetic communities were observed and sampled on six mud volcanoes and along a fault scarp. The communities were dominated by bivalves of particularly small size, belonging to families commonly found at seeps (Mytilidae, Vesicomyidae, Thyasiridae) and to Lucinidae mostly encountered in littoral sulfide-rich sediments and at the shallowest seeps. Siboglinid polychaetes including a large vestimentiferan Lamellibrachia sp. were also associated. At least four bivalve species and one siboglinid are associated with symbiotic chemoautotrophic bacteria, as evidenced by Transmission Electronic Microscopy and isotopic ratio measurements. Among the bivalves, a mytilid harbors both methanotrophic and sulfide-oxidizing bacteria. Video spatial analysis of the community distribution on three volcanoes shows that dense bivalve shell accumulations (mainly lucinids) spread over large areas, from 10% to 38% of the explored areas (2500–15000 m²) on the different volcanoes. Lamellibrachia sp. had different spatial distribution and variable density in the two mud volcano fields, apparently related with higher methane fluxes in the Anaximander volcanoes and maybe with the instability due to brines in the Olimpi area. The abundance and richness of the observed chemosynthetic fauna and the size of some of the species contrast with the poverty of the deep eastern Mediterranean. The presence of a specialized fauna, with some mollusk genera and species shared with other reduced environments of the Mediterranean, but not dominated by the large bivalves usually found at seeps, is discussed.     

Geophysical evidence of mud diapirism on the Mediterranean Ridge accretionary complex

Mud volcanoes, mud cones, and mud ridges have been identified on the inner portion of the crestal area, and possibly on the inner escarpment, of the Mediterranean Ridge accretionary complex. Four areas containing one or more mud diapirs have been investigated through bathymetric profiling, single channel seismic reflection profiling, heat flow measurements, and coring. A sequence of events is identified in the evolution of the mud diapirs: initially the expulsion on the seafloor of gasrich mud produces a seafloor depression outlined in the seismic record by downward dip of the host sediment reflectors towards the mud conduit; subsequent eruptions of fluid mud may create a flat topped mud volcano with step-like profile; finally, the intrusion of viscous mud produces a mud cone.The origin of the diapirs is deep within the Mediterranean Ridge. Although a minimum depth of about 400 m below the seafloor has been computed from the hydrostatic balance between the diapiric sediments and the host sediments, a maximum depth, suggested by geometric considerations, ranges between 5.3 and 7 km. The presence of thermogenic gas in the diapiric sediments suggests a better constrained origin depth of at least 2.2 km.The heat flow measured within the Olimpi mud diapir field and along a transect orthogonal to the diapiric field is low, ranging between 16 ± 5 and 41 ± 6 mW m^–2. Due to the presence of gas, the thermal conductivity of the diapiric sediments is lower than that of the host hemipelagic oozes (0.6–0.9 and 1.0–1.15 W m^–1 K^–1 respectively).We consider the distribution of mud diapirs to be controlled by the presence of tectonic features such as reverse faults or thrusts (inner escarpment) that develop where the thickness of the Late Miocene evaporites appears to be minimum. An upward migration through time of the position of the décollement within the stratigraphic column from the Upper Oligocene (diapiric sediments) to the Upper Miocene (present position) is identified.     

Gas and fluid venting at the Makran accretionary wedge off Pakistan

The Makran accretionary complex shows a distinct bottom-simulating reflector, indicating a thick gas-hydrate-bearing horizon between the deformational front and about 1350 m water depth which seals off the upward flow of gas-charged fluids. A field of presently inactive mud diapirs with elevations up to 65 m was discovered in the abyssal plain seawards of the deformation front, suggesting that in the past conditions were favorable for periodic but localized vigorous mud diapirism. Regional destabilization of the gas hydrate leading to focused flow was observed where deep-penetrating, active faults reach the base of the gas-hydrate layer, as in a deeply incised submarine canyon (2100–2500 m water depth). At this location we discovered seeps of methane and H₂S-rich fluids associated with chemoautotrophic vent faunas (e.g., Calyptogena sp.). Driven by the accretionary wedge dynamics, the landward part of the gas-hydrate layer below the Makran margin is being progressively uplifted. Due to reduced hydrostatic pressure and rising ocean bottom-water temperatures, gas hydrates are progressively destabilized and dissociated into hydrate water, methane and H₂S. Sediment temperatures lie outside the methane stability field wherever water depth is less than 800 m. Above this depth, upward migration of fluids to the seafloor is unimpeded, thus explaining the abundance of randomly distributed gas seeps observed at water depths of 350 to 800 m. Received: 14 June 1999 / Revision accepted: 6 February 2000     

Mud volcanoes and gas hydrates in the Black Sea: new data from Dvurechenskii and Odessa mud volcanoes

Meteor cruise M52/1 documented the presence of gas hydrates in sediments from mud volcanoes in the Sorokin Trough of the Black Sea. In a mud flow on the Odessa mud volcano, a carbonate crust currently forms in association with anaerobic methane oxidation. Dvurechenskii mud volcano (DMV), a flat-topped mud pie-type structure, appeared to be very active. Pore water in sediments of DMV is enriched in several constituents, such as ammonium and chloride, which seem to originate at depth. High sediment temperatures of up to 16.5 °C in close contact to the ambient bottom water of 9 °C also suggest strong advective transport of material from greater depth. Steep temperature gradients indicate a high fluid and/or mud flux within DMV, which is confirmed by the shape of the pore water profiles. Active fluid expulsion sites are evidenced by direct seafloor observation, and a potential flux of methane from the sediment to the bottom water is indicated by water-column methane measurements.     

Mud volcanoes and gas hydrates in the Anaximander mountains (Eastern Mediterranean Sea)

Detailed multibeam, sedimentological, and geophysical surveys provide ample new data to confirm that the Anaximander Mountains (Eastern Mediterranean) are an important area for active mud volcanism and gas hydrate formation. More than 3000 km of multibeam track length was acquired during two recent missions and 80 gravity and box cores were recovered. Morphology and backscatter data of the study area have better resolution than previous surveys, and very detailed morphology maps have been made of the known targeted mud volcanoes (Amsterdam, Kazan and Kula), especially the Amsterdam “crater” and the related mud breccia flows. Gas hydrates collected repeatedly from a large area of Amsterdam mud volcano at a sub-bottom depth of around 0.3–1.5 m resemble compacted snow and have a rather flaky form. New gas hydrate sites were found at Amsterdam mud volcano, including the mud flow sloping off to the south. Gas hydrates sampled for the first time at Kazan mud volcano are dispersed throughout the core samples deeper than 0.3 m and display a ‘rice’-like appearance. Relative chronology and AMS dating of interbedded pelagic sediments (Late Holocene hemipelagic, sapropel layer S1 and ash layers) within the mud flows indicate that successive eruptions of Kula mud volcano have a periodicity of about 5–10 kyrs. New mud volcanoes identified on the basis of multibeam backscatter intensity were sampled, documented as active and named “Athina” and “Thessaloniki”. Gas hydrates were sampled also in Thessaloniki mud volcano, the shallowest (1264 m) among all the active Mediterranean sites, at the boundary of the gas hydrate stability zone. Biostratigraphical analyses of mud breccia clasts indicated that the source of the subsurface sedimentary sequences consists of Late Cretaceous limestones, Paleocene siliciclastic rocks, Eocene biogenic limestones and Miocene mudstones. Rough estimations of the total capacity of the Anaximander mud volcanoes in methane gas are 2.56–6.40 km³.     

Changes in seabed morphology, mud temperature and free gas venting at the Håkon Mosby mud volcano, offshore northern Norway, over the time period 2003–2006

The Håkon Mosby mud volcano is a 1.5-km-diameter geological structure located on the Southwest Barents Sea slope at a water depth of 1,270 m. High-definition seabed mapping of the mud volcano has been carried out in 2003 and 2006. A comparative analysis of the bathymetry and backscatter maps produced from the two surveys shows subtle morphological changes over the entire crater of the mud volcano, interpreted to be the consequence of mud eruption events. Mud temperature measurements point to a persistently warm mud at shallow depth in the crater. This is explained by upward fluid advection, rather than conductive cooling of mud flows. The small-scale spatial variability in the temperature distribution may be related to mud outflows or changes in the fluid flow regime. Furthermore, the locations of free gas venting observed in 2006 were found to differ from those of 2003. Our observations of overall similar topographic profiles across the mud volcano in 2003 and 2006 suggest that eruption events would have been modest. Nevertheless, the data bring evidence of significant change in activity even over short time intervals of only 3 years. This may be a characteristic shared by other submarine mud volcanoes, notably those considered to be in a quiescent stage.     

Comparison of modern fluid distribution,pressure and flow in sediments associated with anticlines growing in deepwater (Brunei) and continental environments (Iran)

Differences in fluids origin, creation of overpressure and migration are compared for end member Neogene fold and thrust environments: the deepwater region offshore Brunei (shale detachment), and the onshore, arid Central Basin of Iran (salt detachment). Variations in overpressure mechanism arise from a) the availability of water trapped in pore-space during early burial (deepwater marine environment vs arid, continental environment), and b) the depth/temperature at which mechanical compaction becomes a secondary effect and chemical processes start to dominate overpressure development. Chemical reactions associated with smectite rich mud rocks in Iran occur shallow (∼1900 m, smectite to illite transformation) causing load-transfer related (moderate) overpressures, whereas mechanical compaction and inflationary overpressures dominate smectite poor mud rocks offshore Brunei. The basal detachment in deepwater Brunei generally lies below temperatures of about 150 °C, where chemical processes and metagenesis are inferred to drive overpressure development. Overall the deepwater Brunei system is very water rich, and multiple opportunities for overpressure generation and fluid leakage have occurred throughout the growth of the anticlines. The result is a wide variety of fluid migration pathways and structures from deep to shallow levels (particularly mud dykes, sills, laccoliths, volcanoes and pipes, fluid escape pipes, crestal normal faults, thrust faults) and widespread inflationary-type overpressure. In the Central Basin the near surface environment is water limited. Mechanical and chemical compaction led to moderate overpressure development above the Upper Red Formation evaporites. Only below thick Early Miocene evaporites have near lithostatic overpressures developed in carbonates and marls affected by a wide range of overpressure mechanisms. Fluid leakage episodes across the evaporites have either been very few or absent in most areas. Locations where leakage can episodically occur (e.g. detaching thrusts, deep normal faults, salt welds) are sparse. However, in both Iran and Brunei crestal normal faults play an important role in the transmission of fluids in the upper regions of folds.     

Vodyanitskii mud volcano,Sorokin trough,Black Sea: Geological characterization and quantification of gas bubble streams

Vodyanitskii mud volcano is located at a depth of about 2070 m in the Sorokin Trough, Black sea. It is a 500-m wide and 20-m high cone surrounded by a depression, which is typical of many mud volcanoes in the Black Sea. 75 kHz sidescan sonar show different generations of mud flows that include mud breccia, authigenic carbonates, and gas hydrates that were sampled by gravity coring. The fluids that flow through or erupt with the mud are enriched in chloride (up to ∼650 mmol L⁻¹ at ∼150-cm sediment depth) suggesting a deep source, which is similar to the fluids of the close-by Dvurechenskii mud volcano. Direct observation with the remotely operated vehicle Quest revealed gas bubbles emanating at two distinct sites at the crest of the mud volcano, which confirms earlier observations of bubble-induced hydroacoustic anomalies in echosounder records. The sediments at the main bubble emission site show a thermal anomaly with temperatures at ∼60 cm sediment depth that were 0.9 °C warmer than the bottom water. Chemical and isotopic analyses of the emanated gas revealed that it consisted primarily of methane (99.8%) and was of microbial origin (δD-CH₄ = −170.8‰ (SMOW), δ¹³C-CH₄ = −61.0‰ (V-PDB), δ¹³C-C₂H₆ = −44.0‰ (V-PDB)). The gas flux was estimated using the video observations of the ROV. Assuming that the flux is constant with time, about 0.9 ± 0.5 × 10⁶ mol of methane is released every year. This value is of the same order-of-magnitude as reported fluxes of dissolved methane released with pore water at other mud volcanoes. This suggests that bubble emanation is a significant pathway transporting methane from the sediments into the water column.     

Reprint of: Comparison of modern fluid distribution,pressure and flow in sediments associated with anticlines growing in deepwater (Brunei) and continental environments (Iran)

Differences in fluids origin, creation of overpressure and migration are compared for end member Neogene fold and thrust environments: the deepwater region offshore Brunei (shale detachment), and the onshore, arid Central Basin of Iran (salt detachment). Variations in overpressure mechanism arise from a) the availability of water trapped in pore-space during early burial (deepwater marine environment vs arid, continental environment), and b) the depth/temperature at which mechanical compaction becomes a secondary effect and chemical processes start to dominate overpressure development. Chemical reactions associated with smectite rich mud rocks in Iran occur shallow (∼1900 m, smectite to illite transformation) causing load-transfer related (moderate) overpressures, whereas mechanical compaction and inflationary overpressures dominate smectite poor mud rocks offshore Brunei. The basal detachment in deepwater Brunei generally lies below temperatures of about 150 °C, where chemical processes and metagenesis are inferred to drive overpressure development. Overall the deepwater Brunei system is very water rich, and multiple opportunities for overpressure generation and fluid leakage have occurred throughout the growth of the anticlines. The result is a wide variety of fluid migration pathways and structures from deep to shallow levels (particularly mud dykes, sills, laccoliths, volcanoes and pipes, fluid escape pipes, crestal normal faults, thrust faults) and widespread inflationary-type overpressure. In the Central Basin the near surface environment is water limited. Mechanical and chemical compaction led to moderate overpressure development above the Upper Red Formation evaporites. Only below thick Early Miocene evaporites have near lithostatic overpressures developed in carbonates and marls affected by a wide range of overpressure mechanisms. Fluid leakage episodes across the evaporites have either been very few or absent in most areas. Locations where leakage can episodically occur (e.g. detaching thrusts, deep normal faults, salt welds) are sparse. However, in both Iran and Brunei crestal normal faults play an important role in the transmission of fluids in the upper regions of folds.     

Chemical and isotopic evidence for the nature of the fluid in CH4-containing sediments of the Håkon Mosby Mud Volcano

The pore waters of CH₄-containing sediments of the Håkon Mosby Mud Volcano were rich in NH⁺ ₄, Br^-, and I^-; exhibited a high total alkalinity; but were poor in Cl^-and SO^2- ₄. The geological evidence and our data suggest that organic matter decomposition in preglacial or early interglacial sediments took place during early diagenesis (bacterial processes) and during metamorphism (thermogenic processes under the 3100-m-thick layer of glacial sediments), accompanied by mud volcano fluid generation. It is argued that the CH₄of the mud volcano sediments has a mixed, biogenic and thermogenic, origin.     

Heat flow in the southern Chile forearc controlled by large-scale tectonic processes

Between 33°S and 47°S, the southern Chile forearc is affected by the subduction of the aseismic Juan Fernandez Ridge, several major oceanic fracture zones on the subducting Nazca Plate, the active Chile Ridge spreading centre, and the underthrusting Antarctic Plate. The heat flow through the forearc was estimated using the depth of the bottom simulating reflector obtained from a comprehensive database of reflection seismic profiles. On the upper and middle continental slope along the whole forearc, heat flow is about 30–60 mW m^–2, a range of values common for the continental basement and overlying slope sediments. The actively deforming accretionary wedge on the lower slope, however, in places shows heat flow reaching about 90 mW m^–2. This indicates that advecting pore fluids from deeper in the subduction zone may transport a substantial part of the heat there. The large size of the anomalies suggests that fluid advection and outflow at the seafloor is overall diffuse, rather than being restricted to individual fault structures or mud volcanoes and mud mounds. One large area with very high heat flow is associated with a major tectonic feature. Thus, above the subducting Chile Ridge at 46°S, values of up to 280 mW m^–2 indicate that the overriding South American Plate is effectively heated by subjacent zero-age oceanic plate material.     

The Norwegian–Barents–Svalbard (NBS) continental margin: Introducing a natural laboratory of mass wasting, hydrates, and ascent of sediment, pore water, and methane

Side-scan sonar mapping and ground-truthing of the Norwegian–Barents–Svalbard continental margin shed new light on shelf glaciation, mass wasting, hydrates, and features like the Håkon Mosby mud volcano (HMMV), reflecting upward mobility of gas, pore fluids, and sediments. Detailed HMMV examination revealed thermal gradients to 10°/m, bottom-water CH₄ and temperature anomalies, H₂S- and CH₄-based chemosynthetic ecosystems, and subbottom methane hydrate (to 25%). Seismic and chemical data suggest HMMV origins at 2–3?km depth within the 6-km-thick depocenter. The HMMV and mound fields bordering the Bjørnøyrenna slide valley and pockmarks bordering the Storegga slide may all have formed in response to sediment failure.     

长江口北槽浮泥周期性变化初探

根据2000年长江口北槽浮泥的实测资料,分析浮泥厚度和体积等几何尺度随时间的变化,发现长江口北槽浮泥存在日、半月和年际三种周期变化;日周期的变化受潮流动力周期的控制,这是由潮流冲刷浮泥形成的悬沙并再絮凝沉积造成的;半月周期的变化受潮流半月周期变化引起的泥沙供给量的变化控制;年周期的变化主要是由影响细颗粒泥沙絮凝沉积的长江口水温的年度变化所控制,泥沙供给和动力的年周期变化也有一定的影响.     

Active mud volcanoes on the upper slope of the western Nile deep-sea fan—first results from the P362/2 cruise of R/V Poseidon

In February 2008, cruise P362/2 was undertaken aboard R/V Poseidon to the Giza and North Alex mud volcanoes (MVs) on the upper slope of the western Nile deep-sea fan. Emitted fluids were strongly depleted in chloride and rich in hydrocarbons, predominantly of thermogenic origin. In-situ sediment temperature measurements indicate extremely high and moderate levels of activity for the North Alex MV and Giza MV, respectively, and suggest rapid changes from dormant to active stages. Both the physical properties of core sediments (e.g., color and magnetic susceptibility), and their assemblages of micro- and nannofossils point to different sources for the two mud volcanoes. Biostratigraphic dating suggests source depths of 2,100–2,450 mbsf for the Giza MV and 1,150–1,550 mbsf for the North Alex MV. Very high temperatures of up to 70°C in shallow sediments at the North Alex MV can be explained only if the fluid source were warmer and deeper than the sediment source.     

Pockmarks off Big Sur, California

A pockmark field was discovered during EM-300 multi-beam bathymetric surveys on the lower continental slope off the Big Sur coast of California. The field contains ∼1500 pockmarks which are between 130 and 260 m in diameter, and typically are 8-12 m deep located within a 560 km² area. To investigate the origin of these features, piston cores were collected from both the interior and the flanks of the pockmarks, and remotely operated vehicle observation (ROV) video and sampling transects were conducted which passed through 19 of the pockmarks. The water column within and above the pockmarks was sampled for methane concentration. Piston cores and ROV collected push cores show that the pockmark field is composed of monotonous fine silts and clays and the cores within the pockmarks are indistinguishable from those outside the pockmarks. No evidence for either sediment winnowing or diagenetic alteration suggestive of fluid venting was obtained. ¹⁴C measurements of the organic carbon in the sediments indicate continuous sedimentation throughout the time resolution of the radiocarbon technique (∼45?000 yr BP), with a sedimentation rate of ∼10 cm per 1000 yr both within and between the pockmarks. Concentrations of methane, dissolved inorganic carbon, sulfate, chloride, and ammonium in pore water extracted from within the cores are generally similar in composition to seawater and show little change with depth, suggesting low biogeochemical activity. These pore water chemical gradients indicate that neither significant accumulations of gas are likely to exist in the shallow subsurface (∼100 m) nor is active fluid advection occurring within the sampled sediments. Taken together the data indicate that these pockmarks are more than 45?000 yr old, are presently inactive, and contain no indications of earlier fluid or gas venting events.     

Dissolved organic carbon in sediments from the eastern North Atlantic

Profiles of dissolved organic carbon (DOC) were measured in the pore water of sediments from 1000, 2000 and 3500 m water depth in the eastern North Atlantic. A net DOC accumulation in the pore waters was observed, which followed closely the zonation of microbial respiration in these sediments. The concentration of pore water DOC in the zone of oxic respiration was elevated relative to that in the bottom ocean water. The resulting upward gradient across the sediment–water interface indicated a steady state diffusive benthic flux, F_DOC, of 0.25–0.44 mmol m⁻² day⁻¹ from these sediments. Subsequent increase in the concentration of DOC in the pore water occurred only in the sediments from 1000 and 2000 m water depth that supported anoxic respiration, leading to a deep concentration maximum. By contrast, in the sediments from 3500 m water depth, a deep concentration minimum was measured, coincident with minimal postoxic respiration in this near-abyssal setting. The gradient-based F_DOC represented approximately 14% of the total remineralized organic carbon (TCR=sum of F_DOC and depth-integrated organic carbon oxidation rate) in the sediments from 1000 and 2000 m water depth, while it was 36% of the TCR in the sediments from 3500 m water depth. A covariance of particulate organic carbon (POC) and pore water DOC with depth in the sediments was evident, more consistently at the deepest site. While the covariance can be related to biotic processes in these sediments, an alternative interpretation suggests a possible contribution of sorption to the biotic control on sedimentary organic carbon cycling. The steady state diagenetic conditions in which this may occur can be conceivable for some organic-poor deep-sea locations, but direct evidence is clearly required to validate them.     

Mud and fluid migration in active mud volcanoes in Azerbaijan

Mud volcanic eruptions in Azerbaijan normally last for less than a few hours, and are characterized by vigorous extrusion of mud breccias, hydrocarbon gases, and waters. Recent fieldwork and mapping on four active mud volcanoes show that dormant period activity ranges from quiet to vigorous flow of mud and fluids. Geochemical analyses of expelled waters show a wide range in solute concentrations, suggesting the existence of a complex plumbing system. The mud and fluids have a deep origin, but are sometimes stored in intermediate-depth mud chambers. A mixing model between deep-seated saline waters and shallow meteoric water is proposed.     

Spatial and seasonal variations of sulphate,dissolved organic carbon,and nutrients in deep pore waters of intertidal flat sediments

Spatial and seasonal variations of sulphate, dissolved organic carbon (DOC), nutrients and metabolic products were determined down to 5 m sediment depth in pore waters of intertidal flats located in NW Germany. The impact of sediment permeability, pore water flow, and organic matter supply on deep pore water biogeochemistry was evaluated. Low sediment permeability leads to an enrichment of remineralisation products in pore waters of clay-rich sediments. In permeable sandy sediments pore water biogeochemistry differs depending on whether tidal flat margins or central parts of the tidal flat are studied. Pore water flow in tidal flat margins increases organic matter input. Substrate availability and enhanced temperatures in summer stimulate sulphate reducers down to 3.5 m sediment depth. Sulphate, DOC, and nutrient concentrations exhibit seasonal variations in deep permeable sediments of the tidal flat margin. In contrast, seasonal variations are small in deep pore waters of central parts of the sand flat. This study shows for the first time that seasonal variations in pore water chemistry are not limited to surface sediments, but may be observed down to some metres depth in permeable tidal flat margin sediments. In such systems more organic matter seems to be remineralised than deduced from surface sediment studies.     

Authigenic carbonates related to active seepage of methane-rich hot brines at the Cheops mud volcano,Menes caldera (Nile deep-sea fan,eastern Mediterranean Sea)

On the passive margin of the Nile deep-sea fan, the active Cheops mud volcano (MV; ca. 1,500 m diameter, ~20–30 m above seafloor, 3,010–3,020 m water depth) comprises a crater lake with hot (up to ca. 42 °C) methane-rich muddy brines in places overflowing down the MV flanks. During the Medeco2 cruise in fall 2007, ROV dives enabled detailed sampling of the brine fluid, bottom lake sediments at ca. 450 m lake depth, sub-surface sediments from the MV flanks, and carbonate crusts at the MV foot. Based on mineralogical, elemental and stable isotope analyses, this study aims at exploring the origin of the brine fluid and the key biogeochemical processes controlling the formation of these deep-sea authigenic carbonates. In addition to their patchy occurrence in crusts outcropping at the seafloor, authigenic carbonates occur as small concretions disseminated within sub-seafloor sediments, as well as in the bottom sediments and muddy brine of the crater lake. Aragonite and Mg-calcite dominate in the carbonate crusts and in sub-seafloor concretions at the MV foot, whereas Mg-calcite, dolomite and ankerite dominate in the muddy brine lake and in sub-seafloor concretions near the crater rim. The carbonate crusts and sub-seafloor concretions at the MV foot precipitated in isotopic equilibrium with bottom seawater temperature; their low δ¹³C values (–42.6 to –24.5‰) indicate that anaerobic oxidation of methane was the main driver of carbonate precipitation. By contrast, carbonates from the muddy lake brine, bottom lake concretions and crater rim concretions display much higher δ¹³C (up to –5.2‰) and low δ¹⁸O values (down to –2.8‰); this is consistent with their formation in warm fluids of deep origin characterized by ¹³C-rich CO₂ and, as confirmed by independent evidence, slightly higher heavy rare earth element signatures, the main driver of carbonate precipitation being methanogenesis. Moreover, the benthic activity within the seafloor sediment enhances aerobic oxidation of methane and of sulphide that promotes carbonate dissolution and gypsum precipitation. These findings imply that the coupling of carbon and sulphur microbial reactions represents the major link for the transfer of elements and for carbon isotope fractionation between fluids and authigenic minerals. A new challenge awaiting future studies in cold seep environments is to expand this work to oxidized and reduced sulphur authigenic minerals.     

Origin of the saline paleofluids in fault-damage zones of the Jabal Qusaybah Anticline (Adam Foothills,Oman): Constraints from fluid inclusions geochemistry

The Jabal Qusaybah Anticline, in north Oman, is affected by syn-folding strike-slip and extensional fault zones developed during foreland deformation ahead of the Northern Oman Mountains thrust wedge, in Cenozoic times. Migration of fluids in fault-damage zones is recorded in complex calcite vein networks. By employing the microthermometric and compositional microanalysis of the fluid inclusions (crush-leach), two distinct generations of veins have been studied. The aim was to determine the source of elevated salinity in fluids involved in their cementation and explain their compositional evolution through fluid-rock interactions. The ionic ratios (Na/Br and Cl/Br) obtained from crush-leach analysis give supporting evidence that the elevated salinity of fluid inclusions in both vein groups originated from an evaporated seawater beyond the onset of halite precipitation (residual brines). The results reveal a gradual increase in salinity of the fluids, F/Cl molar ratios, as well as Li/Cl molar ratios. These results imply the progressively increasing contribution of evaporitic residual brines and fluids that interacted with, or were derived from siliciclastic rocks. We suggest that the most likely origin of the former fluids is provided by residual brines associated with precipitation of the Ara evaporites (Cambrian). The regional driving mechanism for such a significant fluid migration is believed to be compaction-driven upward flow that was channeled into faults and fractures during major deformational events.