Comparative study on the allergenicity of different Litopenaeus vannamei extract solutions

Allergen extracts are widely used for allergy diagnosis and treatment. The application of shrimp extract is hampered due to the low protein concentration and the inconsistent allergenicity. Extracting solutions are considered to be the primary limiting factor of protein extraction from crustaceans. This study aimed to select an optimal solution for shrimp protein extraction by comparing the allergenicity of different shrimp extracts. The effect of 7 existing or modified extracting solutions were evaluated, including the glycerol-NaCl solution, the glycerol Cocaine's solution, the buffered saline solution, the Cocaine's solution, the Glucose leaching solution, 1 mol L-1 KCl solution, and 0.01 mol L-1 phosphate buffered saline solution with and without dithiothreitolor(DTT). The quantitative(protein concentration) and qualitative parameters(SDS-PAGE protein patterns and immuno-reactivity) were determined using the sodium dodecyl sulfate polyacrylamide gel electrophoresis, enzyme linked immunosorbent assay and immunoblotting assay. Results showed that the 1 mol L-1 KCl solution with DTT was optimal for shrimp protein extraction, which yielded high concentration and allergenicity in the protein extract, including major and minor allergens. The 1 mol L-1 KCl solution with DDT is proposed for preparation of shrimp extract and associated allergy diagnosis, as well as potential applications for other crustaceans.     

函数模型与随机模型双约束的GPS-InSAR数据融合方法建立三维形变场

针对InSAR技术研究地表三维形变时监测信息不足的问题,以GPS监测信息为先验信息,建立附有随机模型约束的地表三维形变模型。考虑到SAR卫星极轨方式运行导致LOS向观测量对南北向形变不敏感的问题,以GPS南北向形变观测值作为强约束,构建三维形变解算的函数约束条件。模拟数据与西安地区实测数据的计算结果表明,基于随机模型与函数模型共同约束的地表三维形变参数最小二乘解的精度优于仅有函数模型约束或仅有随机模型约束及无任何约束的参数解精度。     

固定模糊度的GRACE-FO卫星简化动力学定轨精度分析

对GRACE-FO卫星进行精密定轨研究,利用简化动力学方法处理其14 d的星载GPS数据并进行精度分析。通过载波相位残差分析、重叠弧段比较、参考轨道比较以及KBR检核,对比通过UPD方法固定模糊度参数的简化动力学轨道与浮点解轨道。结果表明,GRACE-FO卫星固定解轨道的载波相位残差约为1 cm,比浮点解大1～2 mm。3 d固定解重叠弧段差异的RMS值在R、T、N方向上均小于等于9.4 mm,优于浮点解的12.4 mm。与GFZ提供的精密科学轨道相比,GRACE-C卫星简化动力学固定解轨道在各方向上差异的RMS均值均小于1 cm,表明解算得到的轨道与PSO具有较高的一致性。固定模糊度后K波段测距(K-band ranging)检核残差的RMS均值从9.6 mm下降到6.7 mm,说明固定解能够进一步提升GRACE-FO卫星间的相对位置精度。因此,模糊度固定能够改善GRACE-FO卫星的定轨精度,提供更可靠的轨道服务。     

Theoretical Analysis of Bearing Capacity of Shallowly Embeded Rectangular Footing of Marine Structures

In this paper, the finite element analysis software ABAQUS is used to analyze the ultimate bearing capacity of three-dimensional rectangular footing of marine structures. The deformation law and the failure mode of homogeneous seabed soil beneath the rectangular footing are analyzed in detail. According to the equivalent plastic strain of soil under rectangular footing, an allowable velocity field of homogeneous seabed soil is reasonably constructed. Based on the plastic limit analysis theory of soil mass and by using the Mohr-Coulomb yield criterion, an upper bound solution of the ultimate bearing capacity of three-dimensional rectangular footing on general homogeneous seabed soil is derived, and a correction factor of ultimate bearing capacity of three-dimensional rectangular footing is given. To verify the rationality and applicability of this theoretical solution, some numerical solutions are achieved using the general-purpose FEM analysis package ABAQUS, and comparisons are made among the derived upper bound solution, the solution of Vesic, and the solution of Salgado et al. The results indicate that the upper bound solution of the three-dimensional shallowly embedded rectangular footing proposed in this paper is accurate in calculating the bearing capacity of homogeneous seabed soil. For undrained saturated clay foundation and sandy foundation with smaller internal friction angle, this upper bound solution can evaluate the ultimate bearing capacity of rectangular footing; with the gradual increase of the internal friction angle of the soil, the ultimate bearing capacity of the proposed upper bound solution is slightly higher than that of the rectangular footing.     

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高程异常拟合病态EIV模型的广义岭估计算法

应用法方程矩阵的特征向量构造正则化矩阵,改进通过单一参数既要实现降正则化、又要实现正则化的高程异常拟合病态模型算法。以二次多项式拟合模型为例,应用实测数据对算法进行验证,并与现有病态模型的正则化算法进行比较。     

带耗散项Camassa-Holm方程解爆破的条件

研究了带耗散项Camassa-Holm方程解的性质.通过特征曲线给出了带耗散项Camassa-Holm方程解爆破的条件,并且给出了爆破率的估计.     

AN EXACT SOLUTION OF SUB-INERTIAL FREQUENCY BAROCLINIC WAVES

By using a coordinate transformation, an exact solution of internal tides with sub-inertial frequency isobtained when the bottom slope is linear and the Vaisala frequency is constant. Accordingly thedispersion relations of free waves are presented. This solution is suitable for general coastal low-frequencybaroclinic waves with zero alongshore wavenumber.     

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盐溶液入渗过程的电阻率成像法时空监测

采用电阻率成像法探讨了室内实验尺度上岩体中盐溶液的入渗过程。在岩柱试样上进行蒸馏水和盐溶液的入渗实验,在入渗过程中通过多通道高密度电阻率勘测系统对岩柱的电性特征在三维空间上进行动态监测。然后对入渗过程岩柱进行电阻率成像,从而获得不同时刻岩柱中的电阻率分布。各勘测截面上电阻率的分布和变化基本反映了岩柱内盐溶液的时空变化特征,表明电阻率成像法动态监测室内实验尺度岩石中盐溶液的入渗是可行的,岩柱5个截面的电阻率图像表明溶液的入渗是不均匀的。     

CHARACTER OF SIMPLIFIED NAVIER-STOKES EQUATIONS FOR NEAR THE LEADING EDGE PART OF TWO-DIMENSIONAL LAMINAR FLOW PAST A FLAT PLATE

Starting from the Simplified Navier-Stokes(SNS)equations presented at first by Godovachev-Kuzmin-Tsopov,and Gao Zhi,Davis,the authors analyze the character of the SNS equations for the laminarflow near the leading edge of a flat plate and far away from the plate by using the Weiner—Hopf meth-od and Fourier transform.It is proved that the solution of the SNS equations agree with the solution of the Navier-Stokes equations for flow near the leading edge of the plate and far away from the plate.How to match the solution of the SNS equations to the Blasius solution of the boundary layer equationsis also discussed.     

IMPROVEMENT OF SANDY SOIL WITH WATERCONSERVING MEMBRANE AND ITS EFFECT ON CROP GROWTH

1INTRODUCTIONArid, semi-arid, and semi-humid areas abound in sandysoil, which causes water to leak into deep soil orgroundwater so that soil′s water-conserving capabilitybecomes lower and water is used with low efficiency.Water is one of the important fa…     

地网GeoBeans与高访问量的"SARS网络地理信息系统"

介绍了如何利用地网GeoBeans开发高访问量条件下的“SARS网络地理信息系统”,指出瘦服务器模式是研制具有高访问量特点的网络地理信息系统的最佳解决方案。     

附有等式约束病态模型正则化解的单位权中误差无偏估计

利用平差参数间合理的等式约束虽能提升病态模型解的精度,但其本质仍是通过引入正则化参数来改善模型的病态性,由于改变了观测方程的结构,所得的估值残差及单位权中误差均有偏。针对这一不足,在病态模型正则化解的无偏单位权方差估计式基础上引入等式约束条件,根据约束正则化解的残差二次型期望公式,导出约束正则化解的无偏单位权中误差估计式,并用数值算例和病态测边网算例验证其正确性。结果表明,本文公式所估的单位权中误差精度优于传统公式所估结果。     

文昌A凹陷珠海组储层区域成岩作用特征及定量评价

运用砂岩铸体薄片、扫描电镜、图像分析、孔隙定量分析等资料,研究珠海组深部储层砂岩成岩作用,确定成岩作用横、纵向分布规律;计算成岩参数压实损失的孔隙度和压实率、胶结量、视溶蚀率和溶蚀量,分别对压实作用、胶结作用和溶蚀作用进行定量评价,确定区域成岩作用强度.结果表明:珠海组总体为中等—强压实.其中,凹陷西缘为弱胶结、中等溶蚀;凹陷中心以中等胶结、中等溶蚀为主;南断裂东段下降盘为中等—强溶蚀,珠海组一段和二段为中等—强胶结,珠海组三段为弱—中等胶结;南断裂中段下降盘珠海组一段和二段为中等胶结,珠海组三段为弱胶结,珠海组一段为中等近强溶蚀.     

秩亏自由网平差及其通解

通过坐标转换将初始坐标系下的特解转换得到任意坐标系下的通解,研究了秩亏自由网基准转换的实质。结果表明,秩亏自由网平差最优解实质是基于近似值所确定的基准下的最优解,在实际应用中确定合适的基准是关键。以西安地区GPS沉降监测网为例,不同基准下秩亏解均为该基准下最优解,但只有顾及板块运动的基准才具有物理意义。     

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高频单历元GPS双差解算及其精度分析

基于Windows研制WinTrack高频单历元GPS双差解算软件,进行10 Hz短基线和1 Hz中基线高频观测、动态差分解算及精度分析。试验表明,中短基线条件下,高频单历元GPS双差解的保守精度为cm级,与数据质量、观测条件及时机等因素相关;不同精密星历条件下的解算精度一致,而5 m精度广播星历对短基线结果最大差异小于1 mm。     

螺旋藻制取叶绿素铜钠盐

用乙醇提取螺旋藻中的叶绿素,再用５％ＮａＯＨ溶液皂化、石油醚萃取,在酸性环境中用１０％ＣｕＳＯ４溶液实现铜镁置换,沉淀物经丙配溶解后,加２％ＮａＯＨ的乙醇溶液成盐,过滤,水洗,真空干燥,制成叶绿素铜钠盐．制品的质量达到ＧＢ３２６２－８２的要求．提取率为６４．７％,制品得率占藻粉１．１％。着重对铜镁置换的条件、制品耐酸、耐热、耐光等性能进行了对比试验,结果表明整个工艺是切实可行的．     

Wave interaction with a partially reflecting vertical wall protected by a submerged porous bar

This study gives an analytical solution for wave interaction with a partially reflecting vertical wall protected by a submerged porous bar based on linear potential theory. The whole study domain is divided into multiple sub-regions in relation to the structures. The velocity potential in each sub-region is written as a series solution by the separation of variables. A partially reflecting boundary condition is used to describe the partial reflection of a vertical wall. Unknown expansion coefficients in the series solutions are determined by matching velocity potentials among different sub-regions. The analytical solution is verified by an independently developed multi-domain boundary element method(BEM) solution and experimental data. The wave run-up and wave force on the partially reflecting vertical wall are estimated and examined, which can be effectively reduced by the submerged porous bar. The horizontal space between the vertical wall and the submerged porous bar is a key factor, which affects the sheltering function of the porous bar. The wave resonance between the porous bar and the vertical wall may disappear when the vertical wall has a low reflection coefficient. The present analytical solution may be used to determine the optimum parameters of structures at a preliminary engineering design stage.