Ecological characterization of toxic phytoplankton species (Dinophysis spp., Dinophyceae) in Slovenian mariculture areas (Gulf of Trieste, Adriatic Sea) and the implications for monitoring

Diarrhetic shellfish poisoning (DSP) events are often registered in Slovenian mariculture areas (Gulf of Trieste, Adriatic Sea) and are related to the occurrence of Dinophysis spp. The annual dynamic of this genus and succession of the most important species were studied at two shellfish farms during monitoring fieldwork in the period 1995-2003. Results indicate that the Dinophysis genus maintains a relatively stable inter-annual dynamic at both sites. The Dinophysis community is characterized by two surface maxima in June and September, while in the middle layer only the autumn peak is pronounced (peak median 92 cells l(-1)). Occasional abundance maxima of around 2000 cells l(-1) in the surface layer indicate that potential outbursts of toxic species are less predictable than their seasonal dynamic. On the basis of multivariate analysis, Dinophysis sacculus was characterized as a typical late spring-early summer species, and Dinophysis caudata and Dinophysis fortii as autumn species. Correlation analysis revealed the influence of stratified conditions only on the most abundant species, D. sacculus. Ecological characteristics of the species were combined with shellfish safety requirements towards a more effective monitoring.     

Impact of sampling depth and plant species on local environmental conditions, microbiological parameters and bacterial composition in a mercury contaminated salt marsh

We compare the environmental characteristics and bacterial communities associated with two rushes, Juncus maritimus and Bolboschoenus maritimus, and adjacent unvegetated habitat in a salt marsh subjected to historical mercury pollution. Mercury content was higher in vegetated than unvegetated habitat and increased with sampling depth. There was also a significant relationship between mercury concentration and bacterial composition. Habitat (Juncus, Bolboschoenus or unvegetated), sample depth, and the interaction between both, however, explained most of the variation in composition (~70%). Variation in composition with depth was most prominent for the unvegetated habitat, followed by Juncus, but more constrained for Bolboschoenus habitat. This constraint may be indicative of a strong plant-microbe ecophysiological adaptation. Vegetated habitat contained distinct bacterial communities associated with higher potential activity of aminopeptidase, β-glucosidase and arylsulphatase and incorporation rates of (14)C-glucose and (14)C-acetate. Communities in unvegetated habitat were, in contrast, associated with both higher pH and proportion of sulphate reducing bacteria.     

A comparison of integrated river basin management strategies: A global perspective

In order to achieve the integrated river basin management in the arid and rapid developing region, the Heihe River Basin (HRB) in Northwestern China, one of critical river basins were selected as a representative example, while the Murray–Darling Basin (MDB) in Australia and the Colorado River Basin (CRB) in the USA were selected for comparative analysis in this paper. Firstly, the comparable characters and hydrological contexts of these three watersheds were introduced in this paper. Then, based on comparative studies on the river basin challenges in terms of the drought, intensive irrigation, and rapid industrialization, the hydrological background of the MDB, the CRB and the HRB was presented. Subsequently, the river management strategies were compared in three aspects: water allocation, water organizations, and water act and scientific projects. Finally, we proposed recommendations for integrated river basin management for the HRB: (1) Water allocation strategies should be based on laws and markets on the whole basin; (2) Public participation should be stressed by the channels between governance organizations and local communities; (3) Scientific research should be integrated into river management to understand the interactions between the human and nature.     

离散型湖泊水体提取方法精度对比分析

基于卫星遥感的陆地水体提取方法多种多样,并且应用广泛.对于水体分布支离破碎的枯水期湖泊,准确的水体提取方法尚不明晰,直接影响湖泊水域面积的提取精度.以鄱阳湖湖区为研究对象,利用ALOS遥感影像,以2.5 m高分辨率全色波段融合影像非监督分类(ISODATA)得到的水体面积为参考值,分别使用归一化水体指数(NDWI)法、NDWIISODATA法和基于近红外(NIR)的ISODATA法提取了10 m分辨率的水体分布,分析了不同方法提取结果之间的差异性及产生原因.结果表明:3种方法均可以较好地提取出水体,但利用ISODATA法提取的水体细部信息更为明显,面积值较NDWI法更大;相对于近红外单波段而言,基于NDWI图像的ISODATA法提取水体的精度更高.纵观3种方法,基于NDWI图像的ISODATA法提取的水体精度最高,基于近红外波段的ISODATA法提取结果次之,NDWI阈值法的提取效果最差.研究结果对于离散型湖泊水体提取方法及数据源的选择等具有重要的借鉴和参考意义.     

Selection and criteria for biological indicator species for Arctic monitoring

The estuarine ecosystems are considered as key objects in the Arctic monitoring. Crustacean Saduria entomon, bivalve Portlandia aestuariorum and Cyrtodaria curriana, whitefish Coregonus muksun, C. autumnalis and C. sardinella sardinella and sculpin Triglopsis polaris (= Myoxocephalus quadricornis) are proposed for monitoring of Arctic estuaries. Review of AMAP list of species was carried out. Some species, as included in the World and National ‘Red Books’ and thus being under legislation (polar bear, atlantic and Laptev sea subspecies of walrus, falcon Falco rusticolus) must be left out of the list of species-monitors. The same is applied as in the case of species which often feed on human waste (glaucous gull, fulmar). Instead of using the rare and valuable carnivorous species, appropriately their prey is used as monitors.     

多尺度综合地球物理探测:揭示成矿系统、助力深部找矿——长江中下游深部探测(SinoProbe-03)进展

两个原因使我们必须开展深部探测.一是寻找深部资源,二是更好地理解形成和控制陆内成矿的深部动力学过程,预测新的矿集区.在深部探测专项、国家自然科学基金和地质调查项目资助下,作者在长江中下游成矿带及邻区开展了系统的多尺度、综合地球物理探测.包括:成矿带尺度的宽频地震探测及"廊带式"综合探测,矿集区尺度的骨干剖面探测和三维建模,矿田尺度的三维探测与反演模拟.探测结果在三个层次取得了一系列新认识和新发现,完善了相关探测方法技术.主要包括:揭示了成矿带岩石圈结构、深部过程及对成岩、成矿的控制,提出了成矿带形成的动力学模型,诠释了在狭窄的成矿带内形成大规模金属堆积的深部因素;揭示了典型矿集区地壳三维结构、组成和断裂分布,建立了矿集区三维结构模型和区域成矿模式,推断了主要控矿岩体、地层的空间展布,预测了新的找矿靶区;开展了"玢岩型"、斑岩型和热液型多金属矿床综合地球物理探测试验,总结了勘查模式,预测了深边部找矿靶区;提出了硬岩区反射地震数据采集设计、激发到接收的有效措施,探索了地震弱信号提取、噪声压制、静校正和偏移等处理新技术、新方法;提出了强电磁干扰区电磁去噪的数字形态滤波等技术,完善了二/三维带地形和考虑各向异性情况的电磁正反演技术.     

Potential use of dissolved cyanobacterial DNA for monitoring toxic Microcystis cyanobacteria in filtered water

Toxic and non-toxic Microcystis sp. are morphologically indistinguishable cyanobacteria that are increasingly posing health problems in fresh water systems by producing odours and/or toxins. Toxic Microcystis sp. produces toxicologically stable water soluble toxic compounds called microcystins (MCs) that have been associated with cases of aquatic life and wildlife poisoning and kills including some cases of human illnesses/deaths around the world. Thus, the need for rapid detection of toxic Microcystis sp. in surface water is imperatively a necessity for early mitigation purposes. Genomic DNA from potentially toxic Microcystis sp. comprises of ten microcystin synthetase (mcy) genes of which six major ones are directly involved in MCs biosynthesis. In Polymerase Chain Reaction (PCR) methodsmcy genes can be amplified from intracellular/extracellular genomic DNA using PCR primers. However, little is known about the limitations of sourcing genomic DNA templates from extracellular DNA dissolved in water. In this work, filtered water (0.45 μM) from a Microcystis infested Dam (South Africa) was re-filtered on 0.22 μM syringe filters followed by genomic DNA isolation and purification from micro-filtrates (9 mL). Six major mcy genes (mcyABCDEG) from the isolated DNA were amplified using newly designed as well as existing primers identified from literature. PCR products were separated by gel electrophoresis and visualized after staining with ethidium bromide. The limitation of using dissolved DNA for amplification of mcy genes was qualitatively studied by establishing the relationship between input DNA concentrations (10.0–0.001 ng/μL) and the formation of respective PCR products. The amplification of mcyA gene using new primers with as little as 0.001 ng/μL of DNA was possible. Other mcy gene sensitivities reached 0.1 ng/μL DNA dilution limits. These results demonstrated that with appropriately optimized PCR conditions the method can provide accurate cost-effective tools for rapid detection of toxic Microcystis sp. in water giving early information for water quality monitoring against MC producing cyanobacteria.     

An integrated suspended sediment transport monitoring and analysis concept

A new integrated suspended sediment monitoring strategy applying direct and indirect technologies is presented.Optical sensors continuously record the turbidity at one point in the channel cross section close to the river bank and are calibrated by water samples taken close to the sensor.Additionally measurements are performed to establish the distribution of suspended sediment in a cross section（bottle samples combined with acoustic devices）.Using correction factors（probe and cross-sectional factor） these monitoring methods are combined and it is,thus,possible to fully document the temporal and spatial variability of the suspended sediment transport and to estimate the suspended sediment load for certain time periods.This monitoring strategy was implemented at various measurement sites in Austria as well as at the Hainburg Road Bridge site on the Danube River.It has already been successfully applied for three years at this measurement site and suspended sediment loads during high discharges up to a 15 year flood event have been monitored.To evaluate the new monitoring methods the results were compared with load estimation methods found in the literature including averaging and ratio estimators as well as rating curves.The results prove that with the new methodology,the temporal variability of the suspended sediment transport can be detected more accurately compared with the other methods.They also demonstrate that the additional consideration of the spatial distribution of the suspended sediment concentration in the cross section is crucial as the mean concentration in the cross section can significantly exceed the concentration near the banks,especially at large rivers like the Danube River.     

Time‐integrated sampling of fluvial suspended sediment: a simple methodology for small catchments

Fine‐grained (<62·5 µm) suspended sediment transport is a key component of the geochemical flux in most fluvial systems. The highly episodic nature of suspended sediment transport imposes a significant constraint on the design of sampling strategies aimed at characterizing the biogeochemical properties of such sediment. A simple sediment sampler, utilizing ambient flow to induce sedimentation by settling, is described. The sampler can be deployed unattended in small streams to collect time‐integrated suspended sediment samples. In laboratory tests involving chemically dispersed sediment, the sampler collected a maximum of 71% of the input sample mass. However, under natural conditions, the existence of composite particles or flocs can be expected to increase significantly the trapping efficiency. Field trials confirmed that the particle size composition and total carbon content of the sediment collected by the sampler were representative statistically of the ambient suspended sediment. Copyright © 2000 John Wiley & Sons, Ltd.     

A new and simple method for qualitative sampling of meiobenthos-communities

The introduced collecting method is based on a common entomologist exhauster, which is specified for the fieldwork in aquatic systems with a sieve-inset and a bottom-lid. The living organisms are collected visually from the sampled debris and no further extraction is needed. The apparatus is designed for qualitative sampling of microcrustaceans (e.g. Cladocera, Copepoda, Ostracoda) and minimises disturbance at the sampling site.     

A new collector for sampling volcanic aerosols

A new apparatus, Venturi Effect System (VES), designed for sampling volcanic plumes is described and tested at Vulcano (Italy). This device, together with purified basic NH₄OH solutions, supplies optimal conditions to obtain reliable S_total/Cl/F ratios and enrichment factors for metallic trace elements (MTE). Good concordance for acid gas ratios and metal enrichment factors in both the gas phase and the related plume allows the procedure to be validated. The VES appears in Vulcano conditions as a simple, robust and easily portable apparatus that allows reliable collection of both acid gases and MTE within a single sample and the analysis with current chemical methods (High Pressure Liquid Chromatography, Inductively Coupled Plasma–Mass Spectrometry). This apparatus may be suitable for more difficult volcanoes where only the plume can be sampled.     

基于微服务架构的地震站网全流程一体化监控平台设计与实现

地震站网全流程一体化监控平台是基于微服务框架,应用数据库、通信、并行计算等传统信息技术,结合新兴的云计算、大数据进行建设的信息服务平台。该平台采用Hadoop分布式处理方案,应用Spring Cloud框架搭建,进行Docker容器封装,以标准RESTful API作为服务接口的微服务架构,实现了各应用服务模块之间的高内聚、低耦合及灵活、可扩展的特性,最终实现对地震台站运行的实时监测、高效运维和一体化管理。     

Numerical calculation for bed variation in compound-meandering channel using depth integrated model without assumption of shallow water flow

In a compound meandering channel, patterns of flow structures and bed variations change with increasing water depth owing to complex momentum exchange between high-velocity flow in a main channel and low-velocity flows in flood plains. We have developed a new quasi-three-dimensional model without the shallow water assumption, i.e., hydrostatic pressure distribution; our method is known as the general bottom velocity computation (BVC) method. In this method, a set of depth-integrated equations, including depth-integrated momentum and vorticity equations, are prepared for evaluating bottom velocity and vertical velocity distributions. The objective of this study is to develop a bed variation calculation method for both single and compound meandering channels by using the BVC method coupled with a sediment transport model. This paper shows that the BVC method can reproduce the pattern change of bed variation in a compound meandering channel flow with increasing relative depth. The variation in sediment transport rate due to overbank flow is explained by experimental and computational results.     

Event monitoring of herbicides with naked and membrane-covered Empore disk integrative passive sampling devices

Subsequent to an initial wet season flood event in the Brisbane River, Australia, both fast (naked disk) and slow (membrane-covered) variants of SDB-RPS Empore disk passive sampling devices were deployed with an automated grab sampling program. A trend increase in the aquatic dissolved concentrations of diuron and simazine was observed over a 10-day period. Kinetic and equilibrium parameters for each sampler were calculated based on the dynamic concentration. Absolute percent difference for duplicate passive samples was <10% in the fast and <25% in the slow samplers. For kinetic sampling, significantly shortened integrative periods are available with the fast compared with the slow variant, with higher sampling rates offering improved detection limits. The study demonstrates a method for determining kinetic parameters of passive samplers in a variable concentration field deployment, and illustrates the differences in quality between active and passive data, in terms of capturing changes in concentration associated with rainfall events.     

An integrative method for the evaluation,monitoring, and comparison of seagrass habitat structure

Assessing environmental condition is essential for the management of coasts and their resources, but better management decisions occur when large databases are simplified into more manageable units of information. Here we present the habitat structure index (HSI), which enables rapid assessment and direct comparison of seagrass habitat structure using scores of 0 (poor) to 100 (excellent) based on integrating five habitat variables: area, continuity, proximity, percentage cover, and species identity. Acquiring data to calculate the HSI can be done in situ or from video recordings, and requires relatively simple methodology of belt transects, estimating percentage cover, and basic taxonomy. Spatiotemporal comparisons can usefully identify locations and periods of seagrass habitat change, potentially providing an early warning indicator of habitat damage and decline in environmental quality. Overall, the integrative approach of the HSI represents a step toward simplifying the exchange of environmental information among researchers, coastal managers, and governing bodies.     

Short-term effects of instream habitat restoration on macroinvertebrates and a comparison of sampling approaches

Many streams and rivers worldwide are restored with the intention to mitigate degradation caused by human activities, but these rehabilitation projects often involve physical instream work with diggers or other heavy machinery. The short-term effects of such restoration works on stream ecosystems are rarely investigated. The primary aim of our study was to assess the short-term effects of physical instream restoration works (which likely increased instream fine sediment load temporarily) on the benthic macroinvertebrate community in a lowland soft-bottomed stream, and our second aim was to compare the results of semi-quantitative kick-net and quantitative Surber sampling in this assessment. Invertebrates were collected using these two methods from three Control and three Impact sites, before and two days after recontouring the stream banks and installing instream woody cover features. Three of seven macroinvertebrate community-level indexes suggested positive short-term effects of the restoration works, whereas none indicated negative effects. By contrast, seven of the 14 most common taxa were negatively affected by the restoration works, possibly due to increased fine sediment levels reducing habitat quality for these taxa. These differences imply that taxon-specific invertebrate responses may be more suitable for detecting short-term impacts of instream restoration works than community-level metrics. Community indexes and common taxa were also likely affected by a drought in the spring and summer before restoration, which may have obscured some effects of the restoration works. Finally, kick-net and Surber sampling methods yielded similar findings for the community-level metrics but differed more for the common taxa, probably because the two methods sampled different microhabitats within the stream. Consequently, we recommend that for routine monitoring of macroinvertebrate communities in lowland soft-bottomed streams, the semi-quantitative kick-net sampling method should be used.     

Fractal dimensions of suspended solids in streams: comparison of sampling and analysis techniques

Fluvial suspended sediment typically consists of a variety of complex, composite particles referred to as flocs. Floc characteristics are determined by factors such as the source, size and geochemical properties of the primary particles, chemical and biological coagulation processes in the water column and shear stress and turbulence levels in the stream. Studies of floc morphology have used two contrasting methods of sampling and analysis. In the first method, particles settle on a microscope slide and are observed from below using an inverted microscope. The second method uses filtration at no or low vacuum and particles deposited on the filter are observed with a microscope. Floc morphology can be quantified using fractal dimensions. The aims of the present study were to examine the effect of the two sampling methods on the fractal dimensions of particle populations, and to evaluate for each method how well the fractal dimensions at the various sampling sites reflect basin conditions. Suspended solids were collected in triplicate on inverted microscope slides and on 0·45 μm Millipore HA filters in two southern Ontario streams with contrasting riparian zones during a minor runoff event resulting from the melt of a freshly fallen snowpack. An image analysis system was used to determine area, longest axis and perimeter of particles. The morphology of the particle population of each sample was characterized using four fractal dimensions (D, D₁, D₂ and D_K). Systematic differences in fractal dimensions obtained with the two methods were observed. For the settling method, outlines of larger particles were frequently blurred because of the distance between the focal plane (the top of the inverted microscope slides) and the plane of the particle outline. In this method, the blurring of large particles can cause an increase in the projected area and length of the particle. The effect on the particle perimeter is unpredictable because it depends on the amount of detail lost through blurring and its effect on the apparent increase in particle size. Because of blurring, D and D₁ tend to be systematically lower for the settling method, whereas the net effect on D₂ is unpredictable. Particle size distributions derived from settling are typically coarser because small, low density particles may remain in the water column and all particles may not deposit on the slides. This loss of fines results in systematically lower D_K values for the settling method compared with the filtration method. Fractal dimensions and particle size distributions obtained with the filtration method were sensitive to and clearly indicated differences between drainage basins and between sites within each basin. These differences were explained by basin characteristics and conditions. Fractal dimensions and particle size distributions obtained with the settling method were less sensitive to drainage basin characteristics and conditions, which limits their usefulness as process indicators. Copyright © 1999 John Wiley & Sons, Ltd.     

Optimization of an “in situ” subtidal rocky-shore sampling strategy for monitoring purposes

This study compared 2 standardized protocols to monitor subtidal rocky shores. We tested 2 sampling methods (temporal unit and quadrat) to assess the efficiency of extracting biota parameters (diversity, abundance, and biomass) of macroalgae, Mollusca, and Porifera with respect to time–cost and the number of sampling units. Species richness and occurrence of rocky subtidal habitats were better described by visual censuses than by quadrats. The same estimated richness was provided by the 2 methods. The association of a visual census and a quadrat was the most efficient way for responding to the requirements. A minimum of 5 sampling units per discrete area is recommended for accurately describing habitats. Then, we tested the sensitivity of the proposed protocol on the Bizeux Islet to study the variations of community structures according to depth and station. Based on the results, recommendations for monitoring purposes have been proposed according to European directives.     

Concordance degrees in macrozoobenthic monitoring programmes using different sampling methods and taxonomic resolution levels

In summer 1997, an intensive survey on the hard bottoms of the 'Abra de Bilbao' (N. Iberian Peninsula) was carried out in the context of the macrozoobenthic monitoring programmes developed to assess the biological recovery of the area. Three types of measurements (abundance, biomass and cover) were used to describe and compare the structure and composition of the communities at three littoral zones: subtidal, lower intertidal and upper intertidal. In addition, several taxonomic aggregation levels of data were successfully applied. The main objective of this paper is to explore the relative effect caused on the results of such programmes by earlier decisions concerning the type of measurement and the taxonomic resolution level to be applied. A 'second stage' multivariate procedure of analysis has been perforrmed based on the previously obtained sampling site ordinations. The measurement type chosen has been found to have a greater effect on the results than the taxonomic resolution used. Moreover, it is suggested that analyses based on abundance data usually lose more information when taxonomic resolution decreases than those based on biomass or cover estimates. The highest concordance among the different analyses has been found in the subtidal zone, which is considered the most appropriate habitat for the development of benthic monitoring programmes.     

A simple two-step method for spatio-temporal design-based balanced sampling

We introduce a two-step method to perform spatio-temporal balanced sampling in a design-based approach. For populations with spatio-temporal trends and with anisotropic effects in the variable of interest, the prediction can be further improved by selecting samples that are well spread over the entire population in space and time. We control the spread of the sample over the population by using the volume of the corresponding three-dimensional Voronoi tessellation. Indeed, spatio-temporal design-based balanced sampling is even more efficient under the presence of a trend and anisotropic effects. We present an intensive simulation study comparing our method to other available methods for spatio-temporal sampling. Finally, we analyze real data by sampling from a population of temperature stations over six European countries.