The significance of transparent exopolymeric particles in the vertical distribution of bacteria and heterotrophic nanoflagellates in Lake Pavin

The abundance, size distribution, and bacterial colonization of Transparent Exopolymeric Particles (TEP) were examined in two consecutive years during the spring diatom development throughout the water column of the deep meromictic Lake Pavin, France. TEP concentrations ranged from 1.9 to 13.4 × 10⁵ particles l⁻¹ and their distribution and size spectra indicated that these particles are the main factor in governing the transport of diatoms to the deep hypolimnion of the lake. The majority of TEP was colonized by bacteria that constituted 0.4–8.9% of total DAPI-stained bacteria. The intensity of bacterial colonization was strongly related to temperature and decreased with particle size. A more important colonization of small particles in the hypolimnion during thermal stratification suggested that bacterial colonisation also increased with the age of the particle. The abundance of heterotrophic nanoflagellates (HNF) was more significantly related to the density of particles than to the density of total bacteria and the intensity of bacterial colonization of TEP. Our results therefore suggest that TEP are a more important factor for HNF development than attached and free bacteria. We conclude that TEP are involved not only in sedimentation processes but also in the dynamics of bacteria and protozoa in freshwater pelagic environments.     

Bacterial decay of the autumnal phytoplankton in Lake Constance (Bodensee)

The breakdown of autumnal phytoplankton was studied in Lake Constance by combining chemical seston analyses, bacterial counts and scanning electron microscopy. High algal biomass was associated with low numbers of free-living bacteria. The degradation of algae occurred in two phases: First, dissolved material was released leading to growth of free-living bacteria. Thereafter, bacteria attached to the decaying particles and decomposed them until highly refractory material was left behind. Attached bacteria always comprised less than 25% of total bacteria. Dead organic seston exceeded microbial biomass by a factor of 4–10. Pheopigments increased in senescent and dying algal cells relative to chlorophyll. Therefore, pheopigment fractions were used as a relative index of algal decay.     

Bacteria—particle interactions in turbid estuarine environments

A study of the distribution of bacteria in relation to particle concentration and type was conducted over a spring-neap tidal cycle in the Tamar Estuary, southwest England. Three groups of bacteria were recognized: free-living; those attached to permanently suspended particles; and those attached to particles which undergo tidally controlled resuspension and sedimentation. The total activity and the activity of all three groups of bacteria increase in the turbidity maximum region. The bacteria associated with the permanently suspended particles, which have a larger mean size and organic carbon content than those in the resuspended sediments, contribute the major part of this increased activity. This is a significant finding as it had been previously thought that the increase in bacterial activity at the turbidity maximum was due to bacteria attached to resuspended sediments. However, resuspension still plays an important role because the increase in bacterial activity is consistently coincident with the turbidity maximum.     

Landscape transformation of an Alpine floodplain influenced by humans: historical analyses from aerial images

Natural floodplains are spatially heterogeneous and dynamic ecosystems but at the same time, a highly endangered landscape feature due to climate change and human impacts such as water storage, flood control and hydropower production. Flow is considered a master variable that shapes channel morphology and the heterogeneity, distribution, and turnover of floodplain habitats. Despite their highly dynamic nature, the relative abundance of different habitat elements (islands, gravel bars) in natural floodplains seems to remain relatively constant over ecological periods and is referred to as the shifting mosaic steady state concept. In this conceptual context, we analysed spatiotemporal changes in relative habitat abundance and channel complexity of an alpine floodplain from its near natural state in 1940 before water abstraction and levee construction until 2007 using historical aerial images. Within the first decades of impairment, the relative abundance of floodplain habitats that depend on flood and flow pulses such as parafluvial channels and islands shifted toward a greater abundance of terrestrial forest and grassland habitats. After 1986, the duration and frequencies of high‐precipitation events (>60 mm 24 h^–1) triggering major, channel‐reworking floods increased substantially and caused a restructuring of the floodplain and decrease in the abundance of more terrestrial habitat types. These results are contrary to expectations of the shifting mosaic steady state concept yet suggest its potential application as an indicator of landscape transformation and human impacts on floodplain ecosystems. Last, the results raise the applied question as to whether an increased frequency of high flow events induced by climate change can contribute to floodplain restoration. Copyright © 2011 John Wiley & Sons, Ltd.     

Organic carbon release by phytoplankton and bacterial reassimilation

The release of organic carbon by phytoplankton and its reassimilation by bacteria were studied in Lake Geneva during four daily kinetics, using¹⁴C techniques. Gentamycin was used to suppress bacterial activity. Size fractionation was used to measure¹⁴C fixation in 2 particulate fractions (≥1 μm and 0.2 to 1 μm) and in a third fraction: dissolved organic carbon. Measurements of bacterial biomass and¹⁴C glucose uptake as the H¹⁴CO ₃ ⁻ uptake by samples prefractionated before incubation showed that the whole algae were retained on 1 μm pore size Nuclepore filters, but bacteria were retained on 1 μm and 0.2 μm filters. Concentration of 20 μg.ml⁻¹ gentamycin resulted in incomplete bacterial inhibition while phytoplankton was affected. Phytoplankton released less of 20% of its photoassimilated carbon of which a large proportion was utilized by bacterioplankton.     

Bacterial and Virus‐Like Particle Abundances in Purged and Unpurged Groundwater Depth Profiles

Bacteria and viruses are ubiquitous in subterranean aquatic habitats. Bacterial abundance is known to vary with depth in aquifers; however, whether viral abundance varies with depth is less well known. Here we use flow cytometry (FCM) to enumerate bacteria and virus‐like particles (VLP) from groundwater depth profiles. Groundwater samples were obtained from a set of nested piezometers from depths of 15, 30, 45, 60, 80, and 90 m and bacteria and VLP abundances were determined in purged aquifer water and unpurged water at each slot depth. Mean bacterial abundance (cells / mL) was not significantly different in unpurged water (3.2 × 10⁵) compared to purged water (1.4 × 10⁵); however, mean VLP abundance (particles / mL) was significantly greater in unpurged water (4.4 × 10⁵) compared to purged water (2.3 × 10⁵). Purged water was used to investigate the aquifer depth profile and bacterial and VLP abundances were observed to vary significantly between depths. The virus‐bacteria ratio was determined and was observed to steadily increase with depth. Overall, our data indicate the dynamic nature of bacterial and viral abundances in subsurface environments which should be considered when designing groundwater microbial sampling methodologies.     

The interplay between extrinsic and intrinsic controls in determining floodplain wetland characteristics in the South African drylands

Controls on the characteristics of floodplain wetlands in drylands are diverse and may include extrinsic factors such as tectonic activity, lithology and climate, and intrinsic thresholds of channel change. Correct analysis of the interplay between these controls is important for assessing possible channel–floodplain responses to changing environmental conditions. Using analysis of aerial imagery, geological maps and field data, this paper investigates floodplain wetland characteristics in the Tshwane and Pienaars catchments, northern South Africa, and combines the findings with previous research to develop a new conceptual model highlighting the influence of variations in aridity on flow, sediment transport, and channel–floodplain morphology. The Tshwane–Pienaars floodplain wetlands have formed in response to a complex interplay between climatic, lithological, and intrinsic controls. In this semi‐arid setting, net aggradation (alluvium >7 m thick) in the wetlands is promoted by marked downstream declines in discharge and stream power that are related to transmission losses and declining downstream gradients. Consideration of the Tshwane–Pienaars wetlands in their broader catchment and regional context highlights the key influence of climate, and demonstrates how floodplain wetland characteristics vary along a subhumid to semi‐arid climatic gradient. Increasing aridity tends to be associated with a reduction in the ability of rivers to maintain through‐going channels and an increase in the propensity for channel breakdown and floodout formation. Understanding the interplay between climate, hydrology and geomorphology may help to anticipate and manage pathways of floodplain wetland development under future drier, more variable climates, both in South African and other drylands. Copyright © 2016 John Wiley & Sons, Ltd.     

Microlites and "nanolites" in rhyolitic glass: microstructural and chemical characterization

 We used transmission electron microscopy (TEM) and scanning electron microscopy (SEM) to study magmatic crystals in the Ben Lomond rhyolite lava dome, Taupo Volcanic Center, New Zealand. Using TEM and SEM to investigate the size distributions of these crystals, we identified three size populations: microphenocrysts (>1.2 μm wide), microlites (>0.6 μm wide), and smaller crystals (<0.6 μm wide) which we term "nanolites". The predominant mineral phases of the microlites and nanolites are augites, pigeonites, and hypersthenes. The compositions and microstructures within these pyroxenes indicate disequilibrium crystallization at approximately 850–900  °C and undercoolings as high as 300  °C from equilibrium crystallization temperatures. Complex microstructures resulting from subsolidus reactions in augite and pigeonite are consistent with moderate cooling rates within the upper obsidian layer of the Ben Lomond rhyolite dome. This study demonstrates the existence of sub-micron magmatic crystals in a rhyolite and illustrates the potential of TEM to provide unique information about the crystallization and cooling histories of glassy volcanic rocks. Received: May 8, 1995 / Accepted: November 27, 1995     

Composite suspended sediment particles in river systems: their incidence,dynamics and physical characteristics

Most of the existing data on the effective particle size characteristics of fluvial suspended sediment derive from instantaneous sampling methods that may not be representative of the overall suspended sediment loads. This presents difficulties when there is a need to incorporate effective particle size data into numerical models of floodplain sedimentation and sediment‐associated contaminant transfer. We have used a field‐based water elutriation apparatus (WEA) to assemble a large (36 flood) database on the time‐integrated nature of the effective and absolute particle size characteristics of suspended sediment in four subcatchments of the River Exe basin of southwest England. These catchments encompass a wide range of terrains and fluvial environments that are broadly representative of much of the UK and temperate, low relief northwest Europe. The WEA provides important data on the physical characteristics of composite particles that are not attainable using other methods. This dataset has allowed, for the first time, detailed interbasin comparisons of the time‐integrated particle size characteristics of suspended sediment and reliable estimates of the contribution of five effective size classes to the mean annual suspended sediment load of the study catchments. The suspended sediment load of each river is dominated by composite rather than primary particles, with, for example, almost 60% (by mass) of the sediment load of the River Exe at Thorverton transported as composite particles > 16 µm in size. All the effective size classes contain significant clay components. A key outcome of this study is the recognition that each catchment has a distinctive time‐integrated effective particle size signature. In addition, the time‐integrated effective particle size characteristics of the suspended loads in each of the catchments display much greater spatial variability than the equivalent absolute particle size distributions. This indicates that the processes producing composite particles vary significantly between these catchments, and this has important implications for our understanding of the dynamics of suspended sediment properties. Copyright © 2007 John Wiley & Sons, Ltd.     

The formation of high-grade ignimbrites, I: Experiments on high- and low-concentration transport systems containing sticky particles

 High-grade ignimbrites are thought to be deposited by pyroclastic flows at temperatures exceeding minimum welding temperature or even solidus temperature. Corresponding pyroclastic-flow particles range from plastic to partially liquid and are able to aggregate or coalesce. This contrasts with particles in pyroclastic flows producing unwelded ignimbrite, which are capable of elastic grain interactions. The low aspect ratio and great areal extent of high-grade ignimbrites requires transport in a particulate state either by (a) high-concentration mass flow facilitated by fluidizing gas reducing internal friction, or by (b) expanded turbulent flow of low but downward increasing concentration. This paper presents experiments designed to investigate the effects of plastic to liquid particles on these two contrasting transport mechanisms. Gas fluidization experiments using polyethyleneglycole (PEG) powders heated above minimum sintering (T_ms) and melting (T_m) temperatures cover a wide range of fluidization velocities (U_mf>U_a>0.6·U_t) but are always in the bubbly fluidization regime similar to fluidized ignimbrite ash, where particle volume concentration outside the bubbles is high (≈10^–1). When the powders reach a critical temperature T_m≥T≥T_ms, defluidization by catastrophic particle aggregation immediately commences in both stationary and laterally moving fluidized beds as well as in experiments using mixtures of high- and low-T_m (≥30 wt.%) PEG powders, when T≥T_ms of the lower-T_m powder. This indicates that extended particulate transport at T≥T_ms is not possible at such high particle concentrations. In the turbulent flow experiments, liquid sprays of molten PEG or water, vertically injected into a high-Re (>10⁴) horizontal air flow, form a low-concentration (10^–5 to 10^–4) turbulent suspension current. Proximal formation of partially coalesced aggregates, which settle faster than individual particles, causes the measured downstream decay of sedimentation rate to be steeper than predicted by theory of single solid-particle sedimentation from turbulent suspensions. As particles become finer downstream and coalescence efficiency decreases in response to cooling, more distally formed aggregates become too small and rare to modify sedimentation-rate decay from that of suspension flows containing solid particles. The key difference between the two transport systems is particle concentration, C. Since particle collision rate R_coll∝C², collision rates in fluidized beds are so high that all particles immediately aggregate when coalescence efficiency (1≥E_coal≥0) is larger than 10^-3. Low-concentration suspensions, on the other hand, require much higher values of E_coal for significant aggregation to occur. Dilute pyroclastic flows will have higher particle volume fractions (≈10^–3) than the experimental currents, but then viscous pyroclasts should have lower coalescence efficiencies than PEG droplets. Experimental results thus support an expanded turbulent transport mechanism of pyroclastic flows generating extensive high-grade ignimbrite sheets. Received: 28 August 1996 / Accepted: 3 December 1997     

Partially melted lithic megablocks in the Yardea Dacite, Gawler Range Volcanics, Australia: implications for eruption and emplacement mechanisms

 Lithic megablocks ranging from <1 to 50 m in diameter occur in the Yardea Dacite, a widespread (12,000 km²), thick (>200 m) felsic volcanic unit in the Mesoproterozoic Gawler Range Volcanic Province (GRV) of South Australia. Throughout its vast extent, the Yardea Dacite shows typical lava-like features, in that it is massive, columnar jointed and evenly porphyritic with 30–40% crystals in a spherulitic and granophyric groundmass. In addition, flow banding is present at many locations. The megablocks are abundant at two sites 50 km apart, but isolated megablocks and smaller (<6 cm) lithic clasts are also scattered throughout the unit. At both sites the megablocks are matrix supported, non-graded, randomly oriented and show no evidence of being confined to a particular stratigraphic level in the dacite. The most abundant and largest megablocks are granitoids derived from older basement and from early-crystallised plutons of the Hiltaba Suite, which is broadly coeval and comagmatic with the GRV. The granitoid megablocks have been partially melted, most likely prior to eruption when resident in the thermal aureole of the Yardea Dacite magma chamber. The lithic megablock occurrences are unlike coarse pyroclastic breccias but are similar in distribution and abundance to xenoliths in lavas, consistent with the lava-like character of the host dacite. Using reasonable estimates of megablock density, magma density and magma viscosity, we show that the rise rate of the dacitic magma exceeded the settling velocity of the megablocks, implying that they could have been entrained and erupted effusively. All but the largest and least-melted megablocks would have remained suspended or else settled very slowly in the dacitic lava during outflow. The rapid rate of magma withdrawal required to produce such an extensive felsic sheet could have also triggered disintegration of the thermally stressed wallrock surrounding the magma chamber, dislodging megablocks that were later entrained and effusively erupted. Received: 11 November 1998 / Accepted: 18 April 1999     

Overbank sediment deposition patterns for straight and meandering flume channels

Experiments with the 10 m Flood Channel Facility at HR Wallingford, UK, indicate a fundamental dependency of the overbank deposition pattern of channel suspended sediments on channel planform. Two experiments (100 and 140 l s^?1) in a 1·95 m wide straight channel showed deposition concentrated in a berm along the channel bank. Little sediment was transferred further onto the floodplain. For the larger flow, the berm formed further from the channel. A single experiment (103 l s^?1) with a 1·31 m wide meandering channel showed deposition across the entire floodplain tongue between successive meanders. Maximum deposition occurred on the downstream side of the meander, just past the bend apex. These generalized flume results complement the real‐world but site‐specific data of field studies. Copyright © 2002 John Wiley & Sons, Ltd.     

The particle size characteristics of fine‐grained channel deposits in the River Exe Basin,Devon, UK

Deposition and storage of fine‐grained (<62·5 μm) sediment in the hyporheic zone of gravel bed rivers frequently represents an important cause of aquatic habitat degradation. The particle size characteristics of such fine‐grained bed sediment (FGBS) exert an important control on its hydrodynamic properties and environmental impact. Traditionally, particle size analysis of FGBS in gravel bed rivers has focused on the absolute size distribution of the chemically dispersed mineral fraction. However, recent work has indicated that in common with fluvial suspended sediment, significant differences may exist between the absolute and the in situ, or effective, particle size composition of FGBS, as a result of the existence of aggregates, or composite particles. In the investigation reported in this paper, sealable bed traps that could be remotely opened to sample sediment deposited during specific storm runoff events and a laser back‐scatter probe were used to quantify the temporal and spatial variability of both the absolute and effective particle size composition of FGBS, and the associated suspended sediment from four gravel bed rivers in the Exe Basin, Devon, UK. The absolute particle size distributions of both the FGBS and suspended sediment evidenced c. >95%<62·5 μm sized primary particles and displayed a seasonal winter–summer fining, while the opposite trend was displayed by the effective particle size distribution of the FGBS and suspended sediment. The effective particle size distributions of both were typically highly aggregated, comprising up to 68%>62·5 μm sized particles. Spatial variation in the effective particle size and aggregation parameters was of secondary importance relative to temporal variation. The effective particle size distribution of the FGBS was consistently coarser and more aggregated than the associated suspended sediment and there was evidence of aggregate break‐up in samples of resuspended bed sediment. The implications of these findings for sediment transport modelling are considered. Copyright © 1999 John Wiley & Sons, Ltd.     

Mineralogical analyses and in vitro screening tests for the rapid evaluation of the health hazard of volcanic ash at Rabaul volcano, Papua New Guinea

The continuous ash and gas emissions from the Tavurvur cone in Rabaul caldera, Papua New Guinea, during 2007–08, raised concerns regarding how exposure would affect the respiratory health of nearby populations and impact on the environment. As part of a formal evaluation of the effects of volcanic emissions on public health, we investigated the potential health hazard of the ash using a suite of selected mineralogical analyses and in vitro toxicity screening tests. The trachy-andesitic ash comprised 2.1–6.7 vol.% respirable (sub-4 μm diameter) particles. The crystalline silica content was 1.9–5.0 wt.% cristobalite (in the bulk sample) with trace amounts of quartz and/or tridymite. Scanning electron microscopy showed that the ash particles were angular with sparse, fibre-like particles (∼3–60 μm max. diameter) observed in some samples, which we confirmed to be CaSO₄ (gypsum, at <6 wt.% in the bulk samples) and not asbestiform fibres. The ash specific surface area was low (0.1–2.7 m² g⁻¹). The leached solution from one of the ash samples was slightly acidic (pH 5.6), but did not contain high levels of toxic metals (such as F, Cu, Zn, Mn, As, Ni and Cd) when compared to previously tested volcanic ash leachates. Ash samples generated potentially-harmful hydroxyl radicals through an iron-mediated catalytic reaction, in the range of 0.15–2.47 μmol m⁻² (after 30 min of reaction). However, measurement of particle oxidative capacity (potential oxidative stress reaction using ascorbic acid) and silica-like injury to red blood cells (erythrolysis assay, i.e. measurement of cell death) nevertheless revealed low biological reactivity. The findings suggest that acute exposure to the ash would have a limited potential to exacerbate pre-existing conditions such as asthma or chronic bronchitis, and the potential for chronic exposure leading to silicosis was low.