Biodegradation of dispersed marine fuel oil in sediment under engineered pre-spill application strategy

Biodegradation of marine fuel oil was studied by monitoring changes in residual oil and populations of microorganisms in marine sediments. Biodegradation rates for dispersant and soap water were 2.09 and 2.27 g/kg per day, respectively, under pre-application strategy, suggesting that the strategy may promote MFO dispersion and provide with sufficient source of food. The effect of temperature on the effectiveness of pre-application strategy is particularly obvious for the growth of fungi and Pseudomonas maltophilia. The effect of pre-application of soap water on the tolerance of aerobic bacteria, Escherichia coli, and P. maltophilia, was gradually diminished within 25–33 days.     

Effects of oil and a dispersant on intertidal organisms in field experiments with a Mesocosm,the Bremerhaven Caisson

Three medium-scale field experiments on the effects of oil, a dispersant and an oil/dispersant mixture were carried out in an intertidal mud flat ecosystem of the Wadden Sea (German Bight). For six successive tides each contaminant was added to the water enclosed in a mesocosm during submersion of the flat. The fate of the oil in the sediment and effects on phytobenthos, bacteria and macrozoobenthos were studied. Penetration of the oil into the sediment was mainly observed at the surface layer. were present when oil was chemically dispersed. Sublethal effects were found in some macrofauna species (reduced feeding activity) and in phytobenthic organisms (increased activity); oil degrading bacteria increased. No major effects were observed when the dispersant alone was added.     

Histopathological alterations in Mya arenaria following a # 2 fuel oil spill in the arthur kill, Elizabeth, New Jersey

Following a 2270000-liter #2 fuel oil spill on 1 January 1990 studies were carried out to evaluate histopathological alterations in a population of Mya arenaria inhabiting an impacted tidal mud flat in the Arthur Kill, Elizabeth, New Jersey. Approximately 30 clams were collected 1 week after the spill and on a monthly basis thereafter. The lesion incidence found in M. arenaria following the spill was compared to a base-line incidence established at the same location during 1988 and 1989. There was an increased incidence of moderate and severe lesions of the digestive gland, gills, and mantle 1 week after the spill, returning to pre-spill levels by 1 month. During the first 3 months post-spill an increase in lesion incidence of the intestines was observed which returned to previous levels by 6 months post-spill. The heart and kidney showed a latent increase in lesion incidence 2 and 3 months following the spill, which by 4 months returned to previous levels. Examination of the gonadal development indicated that ova and sperm development were not adversely affected and spawning occurred both in late spring and late summer. The siphon, gill, stomach, foot muscle, and body epithelium were not found to be adversely affected.     

Hydrodynamics of oil jets without and with dispersant: Experimental and numerical characterization

In this paper, we present the analysis of an underwater horizontal oil jet experimental measurement and Computational Fluid Dynamics (CFD) using the Reynolds Averaged Navier Stokes (RANS) equations. Two oil subsurface releases were conducted: one with crude oil and another with crude oil premixed with dispersant at the dispersant to oil ratio (DOR) of 1:20. The jet profile was captured by a camera at moderate resolution, and the instantaneous velocity was measured by a Vectrino Profiler. The velocity components, turbulence kinetic energy, and turbulence dissipation rate from the experiment agreed well with those from the CFD simulation using the k-epsilon turbulence model. The spread angle of the jet was found to be around 21° and 24° from the experiment measurement, for oil without dispersant and oil with dispersant, respectively. The latter is close to the angle of miscible jets at 23°. The jet profile of oil with dispersant had a smaller buoyancy than that without dispersant, which is probably due to the large water entrainment for the oil with dispersant jet. The cross sections of the jet for both cases gradually became flattened with distance, as the plume turned upward.     

横流环境中水下溢油输移扩散的实验研究

文章基于自制的组合式环形水槽（周长9.7 m、宽0.45 m、深1.0 m）,分别以阿曼原油及其消油剂混合物和淡水（含示踪剂）为模拟污染物,开展水下溢油的物理模拟实验,以浮射流输移轨迹、污染物扩散范围和油滴粒径分布为考察指标,研究横流环境和消油剂的使用对水下溢油输移扩散的影响。实验结果表明:在横流环境中,浮射流输移轨迹开始弯曲的高度随着流速的增加而降低;与淡水浮射流主要在水中输移扩散的情况不同,当污染物为原油时,大粒径油滴脱离浮射流主体并上浮至水面,导致扩散范围更大;消油剂的添加会使原油浮射流内部油滴的体积中值粒径变小,油滴粒径分布曲线向小尺寸方向偏移。实验结果可为后续的物理模拟实验和数值模拟研究提供参考。     

Long-term sublethal and short-term high dose effects of physically and chemically dispersed oil on accumulation and clearance from various tissues of juvenile coho salmon, Oncorhynchus kisutch

The toxicity of crude oil and the dispersant BP1100X was tested in sea water and in each case was much less than the toxicity to fish in fresh water. Uptake of oil from water, with or without dispersant, was similar after a short exposure to 200 ppm. The oil was cleared from the fish in three weeks. A long-term exposure to oil or oil plus dispersant at a concentration of 5 ppm showed a slow accumulation but significantly above exposure levels. Thus, salmon exposed to oil in sea water can take up the hydrocarbons but appear to cleanse themselves rapidly.     

南海深水井喷溢油输移扩散的数值模拟研究

文章以假设发生在南海北部深水油气勘探开发海域的1 279 m深水井喷溢油事故为例,采用深海溢油输移扩散模型,以2020年冬季(1月)和夏季(7月)三维流场和海面风场为主要环境动力,数值模拟研究未实施和实施海底消油剂处理情景下的溢油在海洋环境中的输移扩散全过程。模拟结果显示：不同深度的海流对溢油输移轨迹有着显著的影响;深水环境中,流速较小,井喷形成的浮射流能够向上输移较长距离;随着水深变浅,流速增强,流向多变,水中不同粒径的油滴以摇摆或螺旋轨迹向上浮升;在表层海流和冬季或夏季盛行风的共同作用下,海面油膜主要往下风向方向漂移扩散;实施海底消油剂处理措施后,水中油滴扩散范围较未处理时的更大,其中微小粒径油滴将在深水环境中长时间悬浮输移。结果可为今后南海深海溢油输移扩散规律的深入研究提供参考。     

浅海原油净化过程的模拟实验──分散油的挥发降解过程

于1989年3－7月在青岛海洋大学进行“河口”原油和“东营”原油实验室模拟实验，以了解海上溢油进入海水中的分散（溶解和乳化）油的挥发降解过程。结果表明，分散油的蒸发速率除与原油本身性质有关外，还与环境温度、水体盐度和水面风速有关。水体中油含量随时间变化可表示为：，温度、盐度和风速增大时，均有助于分散油的挥发，其中风速变化对Kv的影响最大，而盐度变化的影响最小。     

A chemical investigation of the transport and fate of petroleum hydrocarbons in littoral and benthic environments: The TSESIS oil spill

The fate of saturated and aromatic hydrocarbons discharged into the coastal Baltic Sea environment from the TSESIS oil spill has been studied in the acute and postacute (one year) phases of the spill. Periodic samples of Mytilus edulis (mussels) from eight littoral zone stations and Macoma balthica from nine soft bottom stations were obtained as well as sediment trap samples and surface sediment samples. Glass capillary gas chromatography and gas chromatographic mass spectrometry were used as the analytical tools to determine saturated and aromatic hydrocarbon composition and concentrations in these samples.Sediment trap samples indicated that sizable quantities of chemically and microbially weathered oil were sedimented, and available for benthic uptake shortly after the spill. After initial uptake of sedimented oil (500 to 1000 μ/g dry weight), Macoma populations appear to have begun slow depuration through the first winter after the spill, but TSESIS oil was again introduced to the benthic stations studied during the following summer. Mytilus populations in the region were severely impacted by the oil. Initial depuration of spilled oil during the first month was rapid and nearly complete at all but the most heavily impacted stations one year after the spill. The post-spill depuration of assimilated hydrocarbons was characterised by a relative retention of alkylated dibenzothiophenes and alkylated phenanthrenes compared to their unsubstituted parent compounds and compared with the entire homologous naphthalene series.These data suggest that petroleum hydrocarbons from the TSESIS spill have become a chronic source of degraded saturated and aromatic hydrocarbons to the soft bottom benthic communities. Petroleum hydrocarbons in the benthic environment from this spill appear to reside in the difficulty sampled and mobile flucculent layer at the sediment/water interface and may affect epifaunal communities for an extended period of time.     

Effects of crude oil on the supralittoral meiofauna of a sandy beach

The supralittoral zone of an open sandy beach was dosed with crude oil and changes in the meiofauna monitored. Treatments included weathered and fresh oil on the surface, fresh oil mixed with dispersant on the surface and weathered oil at the water table, all in layers 0·8 cm thick. In all cases the meiofauna was reduced 1 month after dosing but numbers had returned to normal by 5 months except in the site dosed with fresh oil mixed with dispersant. Oil on the surface had greater effects than oil at the water table; fresh oil had a greater effect than weathered oil and fresh oil with dispersant was more toxic than fresh oil alone. Nematodes were least sensitive to the oil and oligochaetes more sensitive, their numbers being correlated with oil concentrations and depth in the substrate. It is concluded that, under all but the heaviest conditions of pollution in their environment, recovery from oil contamination should occur within 5 months in the meiofauna.     

Effects of MV Rena heavy fuel oil and dispersed oil on yellowtail kingfish early life stages

ABSTRACT

The grounding of the MV Rena has highlighted the lack of information concerning the effects of oil-related compounds on New Zealand marine life. Yellowtail kingfish (YTK), Seriola lalandi, embryos were exposed in static incubations to the water-accommodated fraction (WAF) of Rena heavy fuel oil as well as a similar preparation treated with the commercial dispersant Corexit 9500. Mortality in WAF treatments generally increased in association with total polycyclic aromatic hydrocarbon (tPAH) concentration over a 24-h period. Physical abnormalities were observed in some of the larvae exposed to WAF for 48 h. There was no survival in dispersed oil treatments after 24 h of exposure. These treatments had greater tPAH concentrations (2–53?µgL^?1) than equivalent WAF dilutions (0.2–1.5?µgL^?1?tPAH). Indications are that significant morbidity is induced in YTK at ecologically relevant tPAH concentrations. This highlights the need for further research into oil and dispersant toxicity in New Zealand marine species.     

掺水工艺实施后存在问题及解决办法

2007年1月,腰滩油田全面实施掺水工艺。工艺实施初期,因原油物性差、油井分散、单井产量低、新井生产无水采油周期短、油井产液含水率上升快等导致了掺水量过大的问题,使站区日处理液量增加、原油销售超标,严重影响油田的正常生产。为保证掺水工艺在腰滩油田的顺利实施,依据掺水理论,结合油田实际,通过现场简化流程、完善加药方式、制定合理工作制度等措施,使掺水工艺在该油田得到了正常运行,达到了生产稳定、节能降耗、提高经济效益的目的。     

A field study on the acute effects of the dispersant Corexit 9527 on glucose uptake by marine microorganisms

The effects of the dispersant Corexit 9527 and Corexit with crude oil on the rate of glucose uptake and mineralisation were studied in Arctic and Subarctic marine waters and sediments. Essentially all of the 149 water and 95 sediment samples tested displayed decreased glucose uptake rates in the presence of either 15 or 50 ppm Corexit. Depressed uptake rates were observed at concentrations as low as 1 ppm. The mean concentration at which Corexit depressed glucose uptake by 50% was 12 ppm. The effect of Corexit was more pronounced on pelagic than on benthic microbial populations.     

Response of benthic communities in merl experimental ecosystems to low level, chronic additions of No. 2 fuel oil

The macrofauna and meiofauna of three oiled and three control experimental ecosystems at the Marine Ecosystems Research Laboratory (MERL) were followed for 25 weeks of semi-continuous additions of an oil-water dispersion of No. 2 fuel oil. Water column hydrocarbon levels were maintained at about 190 ppb, and after 20 weeks 109 sm/g dry weight fuel oil hydrocarbons were recorded in the top 2 cm of sediment.This simulated chronic oil pollution resulted in a highly significant decline in the number of macrofaunal and meiofaunal individuals in the experimental tanks compared with the controls. The effect was apparent on the numbers of individual macrofaunal species and on all metazoan meiofaunal groups.     

Acute sublethal toxicity of MV Rena contaminants (heavy fuel oil,oil dispersant and cryolite) to finfish and rock lobster

ABSTRACT

The wreck of the MV Rena in October 2011 discharged large quantities of potentially toxic materials in the coastal marine environment of the Bay of Plenty, New Zealand. We evaluated sublethal effects of the water soluble fractions of physically and chemically dispersed heavy fuel oil (HFO) and the water soluble fraction of cryolite on the haematology of subadult or adult snapper (Chrysophrys auratus), spotted wrasse (Notolabrus celidotus) and red rock lobster (Jasus edwardsii). Small but transient effects occurred, the most significant of which were changes in circulating immune cell populations in fish and lobster that recovered by the conclusion of a 96-h exposure period. No post-exposure effects were observed during a 10-day recovery in toxicant-free seawater. Corexit 9500 oil dispersant increased polycyclic aromatic hydrocarbon (PAH) uptake from HFO in fish and lobster by around two-fold and four-fold, respectively, and caused a larger change in immune cell abundance in snapper than exposure to HFO alone. While depuration of PAHs was more rapid in fish than lobster, significantly elevated PAH levels were still evident after 10 days of depuration.     

Some dynamics of spilled oil in a fractured ice field in Buzzards Bay, Massachusetts

A spill of #2 fuel oil occurred 28 January 1977 in a dynamic ice field in BBuzzards Bay, Massachusetts. An oil budget study revealed that within four days after the spill occurred, approximately 60% of the oil was incorporated into the fractured ice zones and onto the ice surface. The movement of the oil (initially spilled under the ice) into the fracture zones appears to be the result of water current below the ice and buoyancy spreading mechanisms. Areas of fracture zones and leads provided a surface outlet for the less dense oil as it moved under the ice. When the leads closed, the oil was trapped into tools on top of the ice at the floe edges. The further spread of the oil onto the surface of the ice floes appears to have been caused by the wind.     

Method for assessing the chronic toxicity of marine and estuarine sediment-associated contaminants using the amphipod Corophium volutator

Acute sediment toxicity tests do not test key life stage events such as moulting and reproduction and therefore do not reveal the longer-term effects of contaminant exposure. A laboratory method is described for determining the chronic toxicity of contaminants associated with whole sediments. The test is conducted using neonates of the estuarine amphipod Corophium volutator at 15 degrees C, salinity 25 psu and a 12 h light:12 h dark photoperiod. The endpoints are survival and growth after 28 days and survival, growth and reproduction of amphipods upon termination of test i.e. reproduction within all control vessels (ca 75 days). The sediment chronic toxicity test was used to investigate the effects of sediments spiked with environmentally relevant preparations of slightly weathered Alaskan North Slope crude oil, including a water-accommodated-fraction (WAF) and a chemically-dispersed (Corexit 9527) WAF. Sediment oil concentrations were quantified using ultra-violet fluorescence. The amphipods exposed to chemically dispersed oil had higher mortality and lower growth rates than control-, Corexit 9527- and WAF-exposed organisms, resulting in reduced reproduction. The described method supplements the standard acute sediment test and would be particularly useful when long-term ecological effects are suspected but acute tests reveal no significant mortality. The sediment chronic test reported herein has shown that sediment that was not evidently toxic during 10-day acute tests could have population-level effects on sediment-dwelling amphipods.     

东太平洋海隆(EPR) 13°N 附近含金属沉积物孔隙率研究

研究了采自东太平洋海隆13°N(EPR 13°N)的一个热液成因含金属箱式沉积物岩心E272的孔隙率变化特征。样品孔隙率在70.0%-85.2%之间,顶部层位比底部层位高14%左右。孔隙率随深度增加而指数式单调递减,平均递减梯度约为-0.31%/cm。该岩心上部层位呈红棕色,下部层位呈黄绿色,表明该岩心经历了显著的早期化学成岩作用。前人关于该岩心的元素地球化学,粒度和年代学数据,共同证明该岩心孔隙率随深度变化特征受稳态压缩过程控制。其孔隙率随深度的变化关系符合稳态压缩模型的经验拟合公式。E272顶部沉积物-水界面处溶解物质与海水的交换以离子扩散机制为主,而岩芯内部溶解物质的迁移则可能主要受自下而上的孔隙水流动机制控制,这与岩芯中活动元素含量上部高,下部低的分布模式一致。该研究对将来进一步讨论E272岩心在早期成岩作用中的化学过程具有指导意义。     

海上油气开发水下井喷溢油的化学分散技术研究

为有效应对海上油气开发水下井喷溢油事故,降低海底溢油对海洋环境的危害,文章通过模拟试验研究消油剂的类型、使用量和喷注位置对溢油分散效果的影响,并提出深水水下消油剂使用技术体系。研究结果表明：GM-2消油剂对原油呈现良好的分散性能,且分散效果随剂油比的增大而增强;水下喷注消油剂的使用量为溢油量的10%时即可获得良好的分散效果;在一定范围内,消油剂喷口与水下井口的水平距离对溢油分散效果的影响极小,而垂直高度对溢油分散效果的影响较大。     

化学消油剂对原油乳化及微生物降解的影响

化学消油剂的使用已成为国内外海洋溢油事故应急处理的重要措施之一。本文分别从光明化学消油剂GM-8的用量、油水比及环境pH等方面,对正十四烷及原油乳化效果进行分析。结果表明, GM-8对正十四烷及原油的乳化效果较好,当化学消油剂的用量为原油的5%时,正十四烷及原油的乳化指数较高。在消油剂与原油用量比为一定值时,原油的乳化效果与海水的量几乎没有关系。环境pH对GM-8乳化原油的影响不大,乳化后的原油可以在海洋环境中稳定7 d以上。GM-8对原油的微生物降解有一定的增强作用。N/P、环境的pH以及微生物的接种量等均对乳化原油的生物降解有较大的影响。当微生物的接种量由培养基的1%增加到10%时,乳化原油的微生物降解率增加了一倍以上,达到91.6%。