Macrobenthic community structure and species composition in the Yellow Sea and East China Sea in jellyfish bloom

To understand the characteristics of macrobenthic structures and the relationship between environment and benthic assemblages in jellyfish bloom, we studied the macrobenthos and related environmental factors in the coastal waters of the Yellow Sea and East China Sea. Data were collected during two seasonal cruises in April and August of 2011, and analyzed with multivariate statistical methods. Up to 306 macrobenthic species were registered from the research areas, including 115 species of Polychaeta, 78 of Crustacea, 61 of Mollusca, 30 of Echinodermata, and 22 of other groups. Nine polychaete species occurred at frequencies higher than 25% from the sampling stations： Lumbrineris longifolia, Notomastus latericeus, Nin6e palmata, Ophelina acuminata, Nephtys oligobranchia, Onuphis geophiliformis, Glycera chirori, Terebellides stroemii, and Aricidea fragilis. Both the average biomass and abundance of macrobenthos are higher in August （23.8 g/m^2 and 237.7 ind./m^2） than those in April （11.3 g/m^2 and 128 ind./m^2）; the dissimilarity ofmacrobenthic structures among stations is as high as 70%. In terms of the dissimilarity values, we divided the stations into four clusters in spring and eight in summer. The ABC curve shows that the macrofauna communities in high jellyfish abundance were not changed. Canonical correspondence analysis showed that depth, temperature, median grain size, total organic carbon of sediment and total nitrogen in sediment were important factors affecting the macrozoobenthic community in the study area.     

Phytoplankton distribution and community structure in the East China Sea (ECS) continental shelf

ＩＮＴＲＯＤＵＣＴＩＯＮＰｈｙｔｏｐｌａｋｔｏｎｈａｓａｋｅｙｒｏｌｅｉｎｔｈｅｍａｒｉｎｅｅｃｏｓｙｓｔｅｍａｓｔｈｅｂａｓｉｃｐａｒｔｉｎｔｈｅｆｏｏｄｃｈａｉｎ ,ｗｈｉｃｈｉｓｉｍｐｏｒｔａｎｔｉｎｆｏｒｍａｔｉｏｎｆｏｒａｓｓｅｓｓｉｎｇｐｒｏｄｕｃｔｉｖｉｔｙｐｏｔｅｎｔｉａｌａｎｄｆｉｓｈｅｒｙｒｅｓｏｕｒｃｅｓ.Ｐｈｙｔｏｐｌａｎｋｔｏｎａｌｓｏｈａｓａｎｉｍｐｏｒｔａｎｔｒｏｌｅｉｎｔｈｅｃａｒｂｏｎｂｉｏｇｅｏｃｈｅｍｉｃａｌｃｙｃｌｅ,ｂｅｃａｕｓｅｉｔｃａｎａｂｓｏｒｂａｌａ…     

Analysis of seasonal variation of water masses in East China Sea

Seasonal variations of water masses in the East China Sea （ECS） and adjacent areas are investigated, based on historical data of temperature and salinity （T-S）. Dynamic and thermodynamic mechanisms that affect seasonal variations of some dominant water masses are discussed, with reference to meteorological data. In the ECS above depth 600 m, there are eight water masses in summer but only five in winter. Among these, Kuroshio Surface Water （KSW）, Kuroshio Intermediate Water （KIW）, ECS Surface Water （ECSSW）, Continental Coastal Water （CCW）, and Yellow Sea Surface Water （YSSW） exist throughout the year. Kuroshio Subsurface Water （KSSW）, ECS Deep Water （ECSDW）, and Yellow Sea Bottom Water （YSBW） are all seasonal water masses, occurring from May through October. The CCW, ECSSW and KSW all have significant seasonal variations, both in their horizontal and vertical extents and their T-S properties. Wind stress, the Kuroshio and its branch currents, and coastal currents are dynamic factors for seasonal variation in spatial extent of the CCW, KSW, and ECSSW, whereas sea surface heat and freshwater fluxes are thermodynamic factors for seasonal variations of T-S properties and thickness of these water masses. In addition, the CCW is affected by river runoff and ECSSW by the CCW and KSW.     

东海中部夏季鱼类群落结构及其多样性分析

根据2008年8月东海区主要渔场重要渔业资源调查资料,分析东海中部鱼类的种类组成、区系特征、多样性等群落结构特征。结果表明:共调查捕获鱼类88种,其中暖水种59种,占67.0%;暖温种29种,占33.0%。相对重要性指数(IRI)值大于500的优势种鱼类共有5种,分别为带鱼、刺鲳、小黄鱼、六斑刺鲀和鳄齿鱼;种类丰富度指数变动范围为0.10～2.31,平均为0.88;Shannon-Wiener多样度指数变动范围为0.01～1.86,平均为0.87;种类均匀度指数变动范围为0.01～0.92,平均为0.41;东海中部夏季鱼类多样性指数与往年相比有下降的趋势。     

Design ofVibrio 16S rRNA gene specific primers and their application in the analysis of seawaterVibrio community

The pathogenic species of genus Vibrio cause vibriosis, one of the most prevalent diseases of maricultured animals and seafood consumers. Monitoring their kinetics in the chain of seafood production, processing and consumption is of great importance for food and mariculture safety. In order to enrich Vibrio-representing 16S ribosomal RNA gene （rDNA） fragments and identify these bacteria further real-timely and synchronously among bacterial flora in the chain, a pair of primers that selectively amplify Vibrio 16S rDNA fragments were designed with their specificities and coverage testified in the analysis of seawater Vibrio community. The specificities and coverage of two primers, VF169 and VR744, were determined theoretically among bacterial 16S rDNAs available in GenBank by using BLAST program and practically by amplifying Vibrio 16S rDNA fragments from seawater DNA. More than 88.3% of sequences in GenBank, which showed identical matches with VR744, belong to Vibrio genus. A total of 33 clones were randomly selected and sequenced. All of the sequences showed their highest similarities to and clustered around those of diverse known Vibrio species. The primers designed are capable of retrieving a wide range of Vibrio 16S rDNA fragments specifically among bacterial flora in seawater, the most important natural environment of seafood cultivation.     

STUDY OF WATER—TRANSPORT THROUGH SOME MAIN STRAITS IN THE EAST CHINA SEA AND SOUTH CHINA SEA

OCCAM global ocean model results were applied to calculate the monthly water transport through 7 straits around the East China Sea(ECS)and the South china Sea(SCS).Analysis of the features of velocity profiles and their variations in the Togara Strait,Luzon Strait and Eastern Taiwan Strait showed that;1)the velocity profiles had striped pattern in the Eastern Taiwan Strait,where monthly flux varied from 22.4 to 28.1 Sv and annual mean was about 25.8 Sv;2)the profiles of velocity in the Togara Strait were characterized by core structure,and monthly flux varied from 23.3 to 31.4 Sv,with annual mean of about 27.9 Sv;3)water flowed from the SCS to the ECS in the Taiwan Strait,with maximum flux of 3.1 Sv in July and minimum of 0.9 Sv in November;4)the flux in the Tsushima Strait varied by only about 0.4 Sv by season and its annual mean was about 2.3 Sv;5)Kuroshio water flowed into the SCS in the Luzon Strait throughout the year and the velocity profiles were characterized by multi-core structure.The flux in the Luzon Strait was minimun in June(about 2.4 Sv)and maximum in February(about 9.0 Sv),and its annual mean was 4.8 Sv;6)the monthly flux in the Mindoro Strait was maximum in December(3.0 Sv)and minimum in June(Only 0.1 Sv),and its annual mean was 1.3 Sv;7)Karimata Strait water flowed into the SCS from May to August,with maximum in-flow flux of about 0.75 Sv in June and flowed out from September to April at maximum outflow flux of 3.9 Sv in January.The annual mean flux was about 1.35 Sv.     

HETEROTROPHIC BACTERIOPLANKTON PRODUCTION IN THE EAST CHINA SEA

ＩＮＴＲＯＤＵＣＴＩＯＮＭｏｒｅｔｈａｎａｄｅｃａｄｅｈａｓｐａｓｓｅｄｓｉｎｃｅｂａｃｔｅｒｉａｗａｓｒｅｃｏｇｎｉｚｅｄａｓｑｕａｎｔｉｔａｔｉｖｅｌｙｉｍｐｏｒｔａｎｔｃｏｎ ｓｕｍｅｒｓｏｆｏｒｇａｎｉｃｃａｒｂｏｎｉｎｍａｒｉｎｅｆｏｏｄｗｅｂｓａｎｄｍａｒｉｎｅｅｃｏｓｙｓｔｅｍｓ (Ｆｕｈｒｍａｎ ,1 992 ) .ＴｈｅｂａｓｉｃｉｎｆｏｒｍａｔｉｏｎｏｎｔｈｅｓｉｇｎｉｆｉｃａｎｃｅｏｆｔｈｅｍｉｃｒｏｂｉａｌｆｏｏｄｗｅｂｗａｓｐｒｅｓｅｎｔｅｄｂｙＰｏｍｅｒｏｙ ( 1 974 ) ,ｗｈｏｐｉｅ…     

Numerical Study of Water and Suspended Matter Exchange Between the Yellow Sea and the East China Sea

INTRODUCTIONTheYellowSeaandtheEastChinaSea (ECS)aremarginalseasofthenorthwestPacificandhaveexpansivecontinentalshelves .TheuniqueandstrikingfeaturesoftheYellowSeaandtheECSarethattheyhavestrongtidalcurrent;aresubjecttostrongmonsooninfluence ;andreceiveinflowfromthebiggestriverinChina ,theChangjiangRiver ;andthatthefamouswesternboundarycurrent,theKuroshio ,passesthroughtheECS ,withitsbranchesintrudingupwardintothecontinentalshelfareas.Generallyspeaking ,thewaterexchangecapacityofthe…     

DISSOLVED ORGANIC CARBON IN THE EAST CHINA SEA IN AUTUMN

ImODUcrIONThepoolofdissolvedorganicrnatter,eva1uatalasdissolvedorganiccarbonpoC),hasbostudndforrnanyycars(Med,l964,SkopintSevCtal.,l966).Continentalmar-ginshavedrihypotheSedtobeasirnPortanttothemarinecarbonbiogeochdricalCy-desasthedeepsea(Walsh,l99l),butinmostdiscussions,onlyexportofparticulateorganieswasconsidetal.HighconcentIationofDOCincoastalwatershasaspedlroleinexportingcarbonfromcontinentalmarginstotheopenocean.UndelstandingtheroleoftheoceanmarginsintheglobalcarbonCycAsdependsla…     

A THREE-DIMENSIONAL THERMOCLINE MODEL FOR THE YELLOW SEA AND NORTHERN EAST CHINA SEA

A time-dependent, three-dimensional finite difference model is presented for simulating the stratifiedYellow Sea and northem East China Sea. The mode is forced by time-dependent observed wind, surfaceflux of heat, and tidal turbulence. With this model, momentum and temperature distribution can be computed,and an approximation for the sub-grid scale effects is introduced by the use of mass and momentumexchange coefficients. The vertical exchanges are quite dependent on these assumed coefficents, whichare complicated functions of the turbulence energy of tide and wind, of the stratified strength and otherfactors. This model was applied to describe the mechanics of the variations in strength and thickness ofthe thermocline covering almost the whole Yellow Sea and northern East Chna Sea in summer. Comparisonsof the computed output with obtained survey data led to some important conclusions.     

STUDY ON THE ANALYSIS AND DISTRIBUTION OF DIMETHYL SULFIDE IN THE EAST CHINA SEA

A method is described for determining dimethylI sulfide (DMS) in seawater. DMS was first extractedfrom the seawater using organic reagent, then reverse-extracted by 5% HgCl2. In the laboratory DMS wasreleased by concentrated HCI and finally measured by GC-FPD. The limit of detection me O.05 ng ofS. Measurements of DMS along surface transects and on wtital profibe across the EaSt China Sca (Ers)continental sheif showed tha itS conodIations of S in the surface seawater ranged from 64-180 ng/L andthat itS vertical djstribuion was divided into 3 trpes. Model talculations of a stagnant film show a DMSflux of 10.6 umol/m_2d the air-sea inteIha.     

Chemical hydrography of coastal upwelling in the East China Sea

Based on the field data obtained during cruises on the shelf of the East China Sea from 1997 to 1999, seasonal variations of coastal upwelling on the inner shelf are discussed by using cross-shelf transect profiles and horizontal distributions of chemical and hydrographic variables. Results show that the coastal upwelling was year-round, but the areas and intensities of the upwelling were quite different in season. The coastal upwelling occurred in all of the coastal areas of the region in spring and summer, but in autumn only in the area off Zhejiang Province, and in winter in the area off Fujian Prov- ince. It was the strongest in summer and the weakest in winter. Geographically, it was the strongest in the area off Zhejiang Province and the weakest in the southmost or northmost parts of the East China Sea. The estimated nutrient fluxes upward into euphotic zone through coastal upwelling were quite large, es- pecially for phosphate, which contributed significantly to primary production and improved the nutrient structure of the coastal ecosystem in the East China Sea.     

Nutrient structure of the Taiwan Warm Current and estimation of vertical nutrient fluxes in upwelling areas in the East China Sea in summer

Based on field data for nutrients collected on the continental shelf of the East China Sea (ECS) during summer 2006, the structure and variations of nutrients in every water mass related to the Taiwan Warm Current (TWC) were analyzed. The supplementary effect of nutrient of upwelling on harmful algal blooms (HABs) in the ECS was also estimated, based on upwelling data. Then the maintenance contribution of nutrient of upwelling to HABs was assessed. The results showed that N/P ratio is fairly low in both surface and deep layers of the TWC, which possibly controls nutrient structure of the HABs-frequently-occuring areas. In upwelling areas, the rate of phosphate (PO₄-P) uptake exceeds that of nitrate (NO₃-N) of the TWC. The TWC may relieve PO₄-P limitation during the process of HABs. Furthermore, upwelling plays an important role in providing nutrients to HABs. After estimating nutrient fluxes (NO₃-N, PO₄-P, SiO₃-Si) in the upwelling areas along a typical section (S07), the results showed that the nutrient uptake rate is the greatest at 10–20 m below euphotic zone, sustaining the ongoing presence of HABs. The uptake rate of PO₄-P is the highest among dissolved inorganic nutrients. Therefore, upwelling is most likely the main source of PO₄-P supply to HABs.     

Tidal analysis in the venturi-shaped area between Zhenhai and Shenjiamen in the East China Sea

In order to better understand the general tidal features in the venturi-shaped area between Zhenhai and Shenjiamen in the northern coastal region of Zhejiang Province in the East China Sea, the tidal data were obtained from both the three permanent tide stations of Zhenhai, Dinghai and Shenjiamen, and four temporary tide stations of Mamu, Chuanshan, Guoju and Liuheng, along with the current speed being observed at Luotou Waterway. Results from harmonic analysis show that: (1) The area was dominated by shallow water tides with irregular semi-diurnal features, and the smallest tidal range occurred in the area near a crossing line between Zhenhai and Dinghai stations, indicating that a tidal node existed in the southern Hangzhou Bay; (2) Formulae, HS2/HM2 >0.4 and gM2-(gK1+gO1)=270° (where H and g are harmonic constants), could be used as judging criteria for high and low tidal level diurnal inequalities; (3) The duration difference between ebb and flood tides could be roughly assessed by the ratio of HM4 vs. HM2; and the larger the ratio is, the bigger the duration difference is. At the same time, the duration period could be assessed by 2gM2-gM4, the epoch difference between M2 and M4 tidal constituents. If 2gM2-gM4 <180°, then the ebb duration is longer than the flood duration; if 180°< 2gM2-gM4 <360°, the result is reversed; (4) Taking Dinghai station as a center point, the highest tidal levels and the average high tidal levels, as well as the average tidal ranges at all stations became higher and larger both southeastwards and northwestwards, while the lowest tidal levels and the average low tidal levels appeared to be lower both southeastwards and northwestwards; and (5) The tidal patterns were not all in line with the tidal current patterns. As a conclusion, the smallest tidal range occurred in the narrow part of the venturi-shaped area. Along the both sides of the area, the highest tidal level and tidal range became higher and larger, while the lowest tidal level became lower with the increase of the distance from the narrow throat area. This is somehow different from the theory that the tidal level increases gradually when it moves towards the top narrow area of a V-shaped bay or estuary.     

Vertical structure of the tidal currents on the continental shelf of the East China Sea

The available data on tidal currents spanning periods greater than six months for the continental shelf of the East China Sea (26°30.052′N, 122°35.998′E) were analyzed using several methods. Tidal Current Harmonic Analysis results demonstrated that semi-diurnal tides dominated the current movement. The tidal currents of the principal diurnal and semidiurnal rotated clockwise with depth, with the deflection of the major semi-axes to the right in the upper layer and to the left in the lower layer. The vertical structures of two principal semi-diurnal constituents-M2 and S2-were similar, which indicates that the tidal currents are mainly barotropic in this area. The main features of the variation of the four principal tidal constituents with depth demonstrate that the currents in this region are influenced by the upper and lower boundary layers. Therefore, the tidal constituents of the shallow water are similar. Different vertical modes were calculated based on the Empirical Orthogonal Function (EOF) analysis of the Eastern and Northern components of the tidal currents, with a variance contribution for the zero-order model of at least 90%. The variance contribution of the baroclinic model is minimal, which further reveals a strong barotropic character for the tidal currents of this region.     

Retrieval of inherent optical properties of the Yellow Sea and East China Sea using a quasi-analytical algorithm

We tested and modified the quasi-analytical algorithm (QAA) using 57 groups of field data collected in the spring of 2003 in the Yellow Sea and East China Sea. The QAA performs well in deriving total absorption coefficients of typical coastal waters. The average percentage difference (APD) is in a range of 13.9%–38.5% for the total absorption coefficient (13.9% at 440 nm), and differences in particle backscattering coefficient bbp(λ) are less than 50% (in the case of the updated QAA). To obtain improved res...     

东海区鲳鱼资源利用现状分析

通过对东海区鲳鱼近年群体组成变化及银鲳生物学特性变化来分析目前鲳鱼资源利用状况,结果表明：一方面,虽然近年鲳鱼产量大幅增加,但其平均体重、优势叉长组成及年龄组成均出现明显下降,资源状况日益恶化;另一方面,1龄鱼已占渔获的93．68％,目前捕捞现行点开捕年龄tc=1和捕捞死亡系数F＝2．51,单位补充量繁殖力相对百分比P＜1．9％,单位补充量渔获量(Yw／R)为70g／尾,均显示已处于严重的生物学型捕捞过度．建议渔业管理部门大力削减作业渔船约50％,严格控制捕捞强度,逐步推迟开捕年龄至3龄以上,使鲳鱼资源真正能够永续利用。     

Distribution of heavy metals and nutrients in rainwater in coastal regions between the southern Yellow Sea and East China Sea

Rainwater samples were collected in series in Qianliyan Island (southern Yellow Sea) and Shengsi Archipelago (East China Sea) between May 2000 and May 2002, chemical analysis for pH values,concentrations of heavy metals (Cu, Pb, Zn and Cd) and nutrients (NH4^-, NO3^-, PO43^-, SiO32^-) were performed.Results indicate that concentrations of most of the heavy metals and nutrients in rainwater show clear seasonal variation, i.e. high level in winter and low level in summer. Regionally, concentrations are higher in the southern Yellow Sea than in the East China Sea, but the annual input of heavy metals into oceans by wet deposition is similar in both stations. However, the input of nutrients by wet deposition in the East China Sea is 2-3 times higher than that in the southern Yellow Sea. In individual, Pb and PO4^3- are input to the sea mainly by dry deposition; whereas Cu, Zn, Cd and N compounds are input dominantly by wet deposition, the N/P ratios in the rainwater from two stations are much higher than those in seawater, showing a significant impact of atmospheric wet deposition on marine production and biogeochemical circulation of nutrients in these sea regions.     

A probe heat flow value of the East China Sea shelf

Bottom temperature variation (BTV) is a serious problem in determining the thermal gradient and heart flow of the sediments in shallow seas. The water depth of the East China Sea shelf is mostly below 150m, and the heat flow measurement is strongly affected by BTV. Following a statistical algorithm, we rechecked the temperature and thermal conductivity data of the cruises KX90-1 and KX91-1, carried out by a cooperation program of China and Japan, and calculate the heat flow in a site without long-term temperature record. The calculated heat flow in the site was 58.6±3.6 mW/m², being just within the range of the drill heat flow value of East China Sea shelf. The inversed amplitude spectrum of BTV has a peak in frequency of 1/10 per year, and the annual component is also an important part. Comparison with two lakes of Lake Greifensee and Lac Leman (i.e. Lake Geneva), which are in different water depth, revealed that with increasing water depth, the peak of amplitude spectrum moved towards low frequency components. The heat flow values calculated in this paper and from petroleum bore hole in East China Sea shelf are much more close to that in southeast China than in Okinawa Trough. Supported by the National High Technology R&D Program of China (2004 AA616060)     

东、黄海鲐鱼群体遗传差异的RAPD分析

利用随机扩增多态DNA(RAPD)技术分别分析了五岛西部日本鲐、东海西部日本鲐和澳洲鲐三群体的样本,共产生226个分子标记,其中143个为多态性标记,多态百分率达到63.3%,每条引物产生的平均标记数为7.29。五岛西部日本鲐、东海西部日本鲐和澳洲鲐群体内遗传相似度分别为0.949、0.877和0.936,而群体间遗传距离依次为:五岛西部日本鲐-东海西部日本鲐(0.2357)<东海西部日本鲐-澳洲鲐(0.3030)<五岛西部日本鲐-澳洲鲐(0.3528),五岛西部日本鲐与东海西部日本鲐个体在UPGMA系统树独立聚类为两个类群,初步支持东、黄海的日本鲐存在东海西部和五岛西部的两个种群的观点。Shannon多样性指数表明,其遗传多样性大小依次为东海西部日本鲐、澳洲鲐、五岛西部日本鲐。