Morphogenetic studies on Uronychia uncinata(Protozoa, Ciliophora) during its asexual division

ＭｏｒｐｈｏｇｅｎｅｔｉｃｓｔｕｄｉｅｓｏｎＵｒｏｎｙｃｈｉａｕｎｃｉｎａｔａ（Ｐｒｏｔｏｚｏａ，Ｃｉｌｉｏｐｈｏｒａ）ｄｕｒｉｎｇｉｔｓａｓｅｘｕａｌｄｉｖｉｓｉｏｎ￥ＳｏｎｇＷｅｉｂｏ（ＲｅｃｅｉｖｅｄＡｕｇｕｓｔ２８，１９９５；ａｃｃｅｐｔｅｄ...     

寡毛双眉虫无性生殖期间的皮膜演化和细胞发生学

于１９９１年６月在青岛海区采集到一腹毛目纤毛虫,寡毛双眉虫。应用蛋白银技术研究了其无性生殖期间的细胞发生学,其特征为：１）ＡＺＭ原基独立地出现于远离模棘毛的前方而非紧靠老的横棘毛附近;２）口棘毛并非来自第一ＦＶＴ原基（在后仔虫）,而是由ＵＭ原基分化而来;３）老的ＡＺＭ将发生后半群的局部重建而非整个地由前仔虫简单继承。     

敏捷瘦体虫(Urosomoida agiliformis FOISSNER,1982)无性生殖期间的形态发生研究(原生动物,纤毛虫)

敏捷瘦体虫（UrosomoidaagiliformisFOISSNER，1982）为小型腹毛目纤毛虫。利用银染法对该种二分裂期间的形态发生学进行初步研究，指出该虫形态发生的主要过程为：1．伴随大核改组带的出现，口原基发生于老口围带的下方，后演化为后仔虫的口围带。在前仔虫，老口围带及口侧膜完全保留并被继承；2．体棘毛场首先出现后一组棘毛原基，后形成前棘毛场，随后各自独立演化成前后仔虫的8：3／4：2／3模式的额-腹-横棘毛；3．在背触毛中，分别于前、后仔虫的中部产生前、后3列新原基，每列原基向其两端伸展替代老背触毛列，成为前、后仔虫相应的3列新触毛；接着，在右缘棘毛原基右前端发生前、后第4列背触毛。     

宽口虫(Steinia sp.)无性生殖周期中核器和纤毛器的演化

利用改进的蛋白银染色法，研究了宽四虫（Steiiniasp．）的形态及其无性生殖周期中核器和纤毛器的发育演化过程。其发生过程为：1．大核改组带出现后，口原基出现在老口围带后方之腹棘毛左侧，其内的毛基体组装成整齐排列的小膜，构成新AZM，老AZM后部也发生部分更新的现象。2．颇腹根棘毛原基各5列，分别以3：3。3：4：3方式分化成前、后仔虫的额、腹、横棘毛。3．在第1－3列背触毛中，分别于前、后仔虫的中部范围产生第1—3列新原基，每列原基向两端伸展替代老背触毛列，成为前、后仔虫相应的第一列新背触毛。4．在前后第3列背触毛原基后端发生前、后第4列原基，它稍偏向第3列原基的右侧，向前伸展成第4列背触毛；在虫体腹面，前、后右缘棘毛原基的前方出现第5、6列背触毛原基，后发育为第5、6列背触毛，在虫体演化过程中转位到背面。     

贻贝棘尾虫形态及二分裂期间形态发生的扫描电镜观察

于１９８６年在哈尔滨郊外水池采集贝棘尾虫标本，利用扫描电镜观察其形态及二分裂期间形态发生，在扫找镜样品制备中，采ＫＭｎＯ４代替常用的ＯｓＯ４研究表明，（１）口原基发生的位置在第一横棘毛的左前角，这下贪食棘尾虫相同，但不同于鬃棘尾虫。参与后仔虫额，腹，横棘毛原基发生的老腹棘毛只有一个，这与前人在其他贻贝棘尾虫株系上的观察结果不同，这些表明，口原基起源的位置和参与形成额、腹，横棘毛原基的老腹棘毛的数目     

Reproductive and growth strategies of Idotea balthica basteri (Pallas, 1772) population in the Bizerte lagoon (Tunisia,Southern Mediterranean)

We analysed several life history traits of the marine isopod Idotea balthica basteri (Pallas, 1772) from the Bizerte lagoon, Southern Mediterranean Sea. Growth was continuous throughout the life of the animal with a high growth rate in the first life phase, and the growth curve was described according to von Bertalanffy's model. The lowest growth rate (0.23 mm) was recorded in winter (December, January and February) and the maximum rate (2.31 mm) between April and June. The total number of hatched eggs or embryos was positively correlated with the body length of ovigerous females. This population of I. balthica basteri was iteroparous, showing distinct strategies of reproduction. Large ovigerous females with high fecundity were collected during the whole sampling period, while breeding in smaller females with low fecundity was restricted to the period from late spring to early autumn, Manca size increased significantly with increasing female body size and there was also a significant trade‐off between manca size and the number of eggs per brood. Reproductive allocation, ranging between 17.1 ± 1.2% in winter and 23.2 ± 1.8% in summer, was positively correlated with female weight. Accordingly, parental investment in producing a juvenile varied between 1.02% per manca in winter to 3.38% in spring. Evaluated traits show that late summer and autumn cohorts have a K‐strategy, whereas cohorts born in winter and spring, and which exhibit a shorter life time, exhibit faster development, earlier reproduction and a smaller parental investment tending towards an r‐selected strategy.     

游仆虫科下阶元系统关系的探讨(纤毛门,腹毛目)

借助聚类分析和近祖演化推测（支序分类方法）对纤毛门原生运行中一高级类群－游仆虫科内各属间系统关系及科内地位予以分析探讨。结果建议：所涉各成员可细分为７个亚群，按演化顺序依次为Ｇａｓｔｒｏｃｉｒｒｈｕｓ；Ｓｗｅｄｍａｒｋｉａ－Ｄｉｓｃｏｃｅｐｈａｌｕｓ；Ｕｒｏｎｙｃｈｉａ；Ｄｉｏｐｈｒｙｓ；Ｅｕｐｌｏｔｉｄｉｕｍ－Ｃｅｒｔｅｓｉａ；Ｃｙｔｈａｒｏｉｄｅｓ－Ｅｕｐｌｏｔｅｓ；Ｅｕｐｌｏｔａｓｐｉｓ－Ａ     

扇贝外寄生车轮虫（Trichodina jadranica Raabe，1958）的形态学研究 （原生动物：纤毛门：缘毛目）

采用Ｗｉｌｂｅｒｔ蛋白银法及Ｃｈａｔｔｏｎ—Ｌｗｏｆｆ银浸法对一种附生于栉孔扇贝鳃丝上的寄生车轮虫进行了光镜水平下的研究。依据其形态学和纤毛图式特征，该种被鉴定为ＴｒｉｃｈｏｄｉｎａｊａｄｒａｎｉｃａＲａａｂｅ，１９５８．此为我国新记录，同时也是第一例有关外寄生于扇贝鳃表纤毛虫原生动物的报道。     

Complementary (secondary) metabolites in a soft coral: sex-specific variability, inter-clonal variability, and competition

Sex‐specific interactions involving competition for space between the dioecious alcyonacean soft coral Sarcophyton glaucum and the scleractinian coral Acropora robusta were assessed experimentally on Bald Rock, central region of the Great Barrier Reef. To examine this, plus inter‐clonal responses, one male colony of S. glaucum, known to produce sarcophytoxide as its predominant complementary (secondary) metabolite, was sectioned, producing 10 clones. The same was done for a female colony. These two sets of clones were then relocated to grids and placed in contact with Acropora clones. Relocated and non‐relocated controls were also monitored. High levels of tissue necrosis were observed in the hard coral under contact conditions with both the male and female clones after 20 days. The development of a protective polysaccharide layer in the alcyonacean was also observed. Differences observed in the concentrations of complementary metabolites within the two different S. glaucum colonies were related to sex. Both under competition and non‐competition conditions, females exhibited significantly higher concentrations of sarcophytoxide than males, and this increased with time. Fatty ester concentration was also higher in females than males, varying significantly through time, and falling dramatically just after spawning. Fatty ester concentrations decreased linearly through time in the male clones. When involved in competition for space, females possessed higher concentrations of fatty esters than males, both at the site of contact and in non‐contact sites, again, decreasing after spawning. No significant changes in sarcophytoxide levels were noted in the parental colonies, but such changes were observed in fatty esters, with the female producing higher concentrations until after spawning. The use of these two variates in the form of a ratio (sarcophytoxide concentration:fatty ester concentration) yielded a variable Rho (ρ) which was a more sensitive indicator of biochemical change than either of its components alone. These two sets of compounds appeared to have a negative association through time and varied highly significantly between sexes. The diterpene sarcophytoxide may be considered an allelopathic or stress metabolite, while the lipids act as energy storage metabolites.     

Tectonics of an extinct ridge-transform intersection,Drake Passage (Antarctica)

New swath bathymetric, multichannel seismic and magnetic data reveal the complexity of the intersection between the extinct West Scotia Ridge (WSR) and the Shackleton Fracture Zone (SFZ), a first-order NW-SE trending high-relief ridge cutting across the Drake Passage. The SFZ is composed of shallow, ridge segments and depressions, largely parallel to the fracture zone with an `en echelon' pattern in plan view. These features are bounded by tectonic lineaments, interpreted as faults. The axial valley of the spreading center intersects the fracture zone in a complex area of deformation, where N120° E lineaments and E–W faults anastomose on both sides of the intersection. The fracture zone developed within an extensional regime, which facilitated the formation of oceanic transverse ridges parallel to the fracture zone and depressions attributed to pull-apart basins, bounded by normal and strike-slip faults.On the multichannel seismic (MCS) profiles, the igneous crust is well stratified, with numerous discontinuous high-amplitude reflectors and many irregular diffractions at the top, and a thicker layer below. The latter has sparse and weak reflectors, although it locally contains strong, dipping reflections. A bright, slightly undulating reflector observed below the spreading center axial valley at about 0.75 s (twt) depth in the igneous crust is interpreted as an indication of the relict axial magma chamber. Deep, high-amplitude subhorizontal and slightly dipping reflections are observed between 1.8 and 3.2 s (twt) below sea floor, but are preferentially located at about 2.8–3.0 s (twt) depth. Where these reflections are more continuous they may represent the Mohorovicic seismic discontinuity. More locally, short (2–3 km long), very high-amplitude reflections observed at 3.6 and 4.3 s (twt) depth below sea floor are attributed to an interlayered upper mantle transition zone. The MCS profiles also show a pattern of regularly spaced, steep-inclined reflectors, which cut across layers 2 and 3 of the oceanic crust. These reflectors are attributed to deformation under a transpressional regime that developed along the SFZ, shortly after spreading ceased at the WSR. Magnetic anomalies 5 to 5 E may be confidently identified on the flanks of the WSR. Our spreading model assumes slow rates (ca. 10–20 mm/yr), with slight asymmetries favoring the southeastern flank between 5C and 5, and the northwestern flank between 5 and extinction. The spreading rate asymmetry means that accretion was slower during formation of the steeper, shallower, southeastern flank than of the northwestern flank.     

Morphology of a Pre-collisional, Salt-bearing, Accretionary Complex: The Mediterranean Ridge (Eastern Mediterranean)

The Eastern Mediterranean Sea is a remnant of a deep Mesozoic oceanic basin, now almost totally consumed as a result of long-term plate convergence between Eurasia and Africa. The present-day surface morphology of the Eastern Mediterranean relates both to the early history of formation of the deep basins and the recent geodynamic interactions between interfering microplates. Among the most conspicuous morphologic features of the basin is an arc-shape, elongated and wide, bathymetric swell bisecting the entire basin from the Ionian to Levantine areas, known as the Mediterranean Ridge. During the last decade this tectono-sedimentary accretionary prism, which results from the Hellenic subduction, has been intensively surveyed by swath mapping, multichannel seismic profiling and deep dives. We present here, and briefly discuss, the main morphological characteristics of this feature as derived from swath bathymetric data that considerably help to better assess the lateral and north–south morphostructural variability of the Mediterranean Ridge. This study reveals that the characteristics and morphostructural variability of the Mediterranean Ridge are related to: (1) a specific incipient collision geodynamic setting south of Crete, where the African and Aegean continental margins are nearly in contact, (2) a unique regional kinematics, controlled by frontal convergence south of Crete (central Mediterranean Ridge) and oblique subduction with opposite sense of shear for the western (Ionian) and eastern (Levantine) domains of the Mediterranean Ridge, that explain the lateral variations of deformation and (3) particularities of its sedimentary cover, which includes massive salt layers within the outer Mediterranean Ridge and local salt deposits within the inner domains, that control the north–south morphostructural variability of the sedimentary wedge.     

Distribution and Adaptations of Sea Ice Inhabiting Harpacticoida (Crustacea, Copepoda) of the Weddell Sea (Antarctica)

Abstract. Seven harpacticoid species were found to be associated with sea ice collected during 5 Antarctic cruises of the RV Polarstern. Their distribution within the fast ice and pack ice zones of the Weddell Sea is presented. Whereas some individuals probably become entrapped into the ice only accidentally, at least 4 species arc genuinely sympagic. These have evolved remarkable adaptations, as evidenced by field and laboratory studies of Dresclieriella glacialis , which is by far the most abundant. Such adaptations include: the ability to penetrate deep into ice; a comparatively high salinity tolerance allowing it to endure large salinity fluctuations associated with ice crystal formation and melting; good swimming ability, necessary both for horizontal dispersal and for a planktonic intermezzo after annual melting of the sea ice. D. glacialis is the first polar non-vertebrate mctazoan to be cultivated through its entire life cycle. Its life history suggests an r-stratcgy; this would be the first indication of such a trait in the polar environment. A new species of Drescheriella , on the other hand, exhibits resting stages (C IV-V) known to date only for Calanoidu but not reported for Harpacticoida.     

Effect of siderophores on the light-induced dissolution of colloidal iron(III) (hydr)oxides

Siderophores play an important role in biological iron acquisition in iron-limited aquatic systems. While it is widely accepted that the solubilization of iron-bearing mineral phases is a key function of siderophores, the mechanism of siderophore-promoted mineral dissolution in aquatic systems is largely unknown. In this study, we investigated the effect of siderophores (desferrioxamine B (DFOB) and aerobactin) on light-induced dissolution of goethite and lepidocrocite in the presence or absence of oxalate in aerated and deaerated suspensions at pH 6. For the irradiated two-ligand system (oxalate/siderophore), the experimental results suggest that oxalate acts as the electron donor for the formation of surface Fe(II), and the siderophore acts as an efficient shuttle for the transfer of surface Fe(II) into solution. Furthermore, even in the absence of an electron donor such as oxalate, both DFOB and aerobactin accelerated the light-induced dissolution of lepidocrocite as compared to the thermal dissolution. Experiments with dissolved Fe(III)–DFOB and Fe(III)–aerobactin complexes suggest that this enhancing effect is not due to photolysis of corresponding surface complexes but to efficient transfer of reduced surface Fe(II) into solution, where surface Fe(II) may be formed, e.g., through photolysis of surface Fe(III)–hydroxo groups. Based on this study, we conclude that the interplay of light and siderophores may play a key role in the dissolution of colloidal iron(III) (hydr)oxides in marine systems, particularly in the presence of efficient electron donors.     

A string of large and deep gas-induced depressions (pockmarks) offshore Killini peninsula, western Greece

A string of five large depressions (pockmarks) has been discovered offshore Killini peninsula in western Greece. They can be considered as some of the deepest (61-m maximum detected depth), if not the deepest observed on the seafloor world-wide. The pockmarks erode deeply buried compacted sediments and are modified by sliding. Their formation is attributed to gases which were detected in the subbottom sedimentary layers. Gas origin is considered to be both biogenic and thermogenic as gas was detected geochemically in exploratory wells in the neighbouring Katakolon area. Although uncertainties concerning their mode of formation, age and activity still remain, it is believed that the high seismicity and salt tectonics of the region contributed to the violent eruption and escape of large quantities of gases through small faults or joints which enabled erosion deep into the seafloor.     

On the measurement of socioeconomic benefits of integrated coastal management (ICM): Application to Xiamen,China

We develop a systematic approach for the measurement of overall socioeconomic benefits associated with an integrated coastal management (ICM) program. The analytical framework includes multiple marine industry sectors (e.g., ocean shipping and commercial fisheries) as well as environmental sectors (e.g., coastal erosion). The net benefit measure captures both economic and environmental effects. We apply our analytical model to Xiamen, China, using empirical data from 1992 to 2001. Results of the case study show that the implementation of ICM program in Xiamen has led to a significant increase (over 40%) in annual socioeconomic benefit from its marine sectors. Thus, the Xiamen ICM program has been effective in achieving sustainable development.     

Genesis and evolution of hydrocarbons entrapped in the fluorite deposit of Koh-i-Maran, (North Kirthar Range, Pakistan)

The exceptional development of coeval hydrocarbon and aqueous fluid inclusions (FI) in fluorite from the MVT-type ore deposit of Koh-i-Maran, Baluchistan (North Kirthar range, Pakistan), provides samples which are representative of the ore-forming fluid and which support the hypothesis of petroleum migration in the province. Primary brines at 125°C (10 wt% equ. NaCl) and secondary CH₄-rich brines at 135°C (7 wt% equ. NaCl), are recognised to be associated with oil migration in the fluid inclusions. They support the model of a per ascensum MVT (Mississippi Valley Type) stratabound hydrothermal deposit. A pressure–temperature path of 120–125°C to 165–200 bars is calculated from microthermometric data and PVT modelling of hydrocarbon FI using the modified Peng–Robinson Equation of State (IFP software) from primary cogenetic inclusions (oil and brines).The composition of gas and oil fractions is obtained by a combination of Synchrotron FTIR microanalysis and gas chromatography performed on individual fluid inclusions. The oil entrapped as a coeval primary fluid phase is a light aliphatic normal oil in the range C₈–C₃₅ with a high CO₂ content. The brown solid phase found systematically in the oil is probably asphaltene resulting from precipitation after trapping of the heavy fraction, which commonly occurs by decreasing pressure and temperature and\or by CO₂ injection. Later CH₄-rich brine influx probably modified part of the oil in the primary fluid inclusions because degraded oil is observed within such inclusions. Biomarkers obtained by GC-MS analysis indicate a terpane distribution quite similar to the nearest oil seepage in the Gokurt area. This result and the high CO₂ content of organic fluid inclusions indicate a restricted/confined sedimentary environment for the source rock, which could correspond to the Eocene Carbonate formation with type-II organic matter. A possible additional input of gas from the Sambar formation is suggested as feasible. The link between the fluid inclusion data and the geodynamic evolution lead us to propose a circulation of basinal fluids driven mainly by the fault system during dewatering in the foredeep. In Pakistan, they are coeval to major compressional NW–SE Oligocene episode in the thrust belt. The origin of the fluorine may be found in the basin sediments as well as near the basement. The brines originated in salt structures recognized in eocambrian at the decollement level, the source rock was already mature.     

Larval fish assemblages,environment and circulation in a semienclosed sea (Gulf of California,Mexico)

Fish larvae and hydrographic data collected in the Gulf of California (GC) in December 2002 are used to describe larval fish assemblages (LFAs) and to explore their relationships with environmental variables (temperature, salinity, dissolved oxygen, fluorescence maximum, ?$?$ and superficial chlorophyll a). The Bray–Curtis dissimilarity index defined three LFAs, distributed in areas with distinctly different environmental conditions. The affinity of most of the species with the environmental characteristics of their areas of distribution could be interpreted as an indication that spawning occurred inside those areas. Particle tracking in current fields from a 3D numerical model and connectivity matrices are used to assess larval retention in the LFA areas. The technique is well suited for seas like the GC that have well-defined circulation patterns. On time scales around 30 days, retention (from 56% to 73% of the particles) occurred (1) for the North LFA in the Upper GC, (2) for the Channel-Center LFA in the anticyclone over the Northern GC and in Ballenas Channel, and (3) for the South LFA in the eddy over San Pedro Mártir basin and in the shallow zone off the peninsula. Therefore, the Lagrangian analysis revealed that the observed LFAs have a permanency long enough to allow fish larvae to remain in a favorable environment until they develop motility. The main particle export path (less than 26% of the particles) was from the North to the South LFA, following the anticyclonic main flow and coinciding with the gradient in species number and larval abundance.     

Life history of Galaxias divergens (Salmonoidea: Galaxiidae)

Galaxias divergent Stokell is a small, bottom‐dwelling fish inhabiting streams in the North Island and the northern part of the South Island. Its ecology is similar in many respects to that of G. vulgaris. The fry develop in the same environment as the adults, unlike the sea‐going young of several other Galaxias spec'es. Spawning occurs in spring and summer, and at least some of the fish mature at the beginning of their second year. The maximum age attained seems to be about 3 years, and the fish can reach a length of about 80 mm in this time. The food consists mostly of small benthic invertebrates taken from both the stream bed and the drift fauna.     

Distrubution of Dissolved and Particulate Iron, copper and Manganese in the Shelf waters of the Ross sea (Antarctica)

Abstract. During the austral summer 1997–98, within the framework of the activities of the Climatic Long-term Interaction for the Massbalance in Antarctica (CLIMA) Project of the Italian National Program for Antarctic Research (PNRA) in the Ross Sea, measurements were conducted to focus on the role of dissolved iron, copper and manganese as micronutrients, and on their distribution in suspended particulate matter in different water masses. Sampling was carried out in two selected shelf areas, both important for formation and mixing processes of the water bodies.
Metal data were evaluated together with physical measurements and classical chemical parameters such as oxygen and nutrients.
In both the studied areas, the distribution of dissolved metals along the waste column confirmed their micronutrient behaviour, showing depletion where phytoplanktonic activities occurred.
The trend of particulate metals underlined the scavenging phenomena along the water column and presented an interesting correlation at intermediate depths with the amount and origin of suspended matter.     

Reproductive biology of Parazoanthus axinellae (Schmidt, 1862) and Savalia savaglia (Bertoloni, 1819) (Cnidaria,Zoantharia) from the NW Mediterranean coast

Despite the wide distribution of zoanthids, little is known about their pattern of reproduction. Here we investigate the reproductive biology of two Mediterranean species, the common Parazoanthus axinellae (Schmidt) and the rare Savalia savaglia (Bertoloni). For both species, samples were collected during an annual cycle, from January to December 2005, in the Western Mediterranean (Ligurian Sea, Italy). Both species are gonochoric. In P. axinellae the sex‐ratio (n colonies = 30) showed a slight predominance of male colonies (M/F = 1.35), whereas in the population of S. savaglia (n colonies = 15) a predominance of females was found (M/F = 0.3). In P. axinellae the first gametocytes were visible in March, whereas in S. savaglia they became visible in May. Both species reproduce at the end of autumn when seawater temperature begins to decrease. Parazoanthus axinellae (10 m depth) spawns eggs and sperms in November, whereas S. savaglia (67 m depth) spawns in December. In P. axinellae sexes were segregated on a rocky wall, with males occurring deeper, whereas male and female colonies of S. savaglia were irregularly dispersed in the population. The maximum number of oocytes differed between the species, being higher in P. axinellae than in S. savaglia.