An electrochemical RNA hybridization assay for detection of the fecal indicator bacterium Escherichia coli

Monitoring waters for indicator bacteria is required to protect the public from exposure to fecal pollution. Our proof-of-concept study describes a method for detecting fecal coliforms. The coliform Escherichia coli was used as a model fecal indicator. DNA probe-coated magnetic beads in combination with the electrochemical monitoring of the oxidation state of guanine nucleotides should allow for direct detection of bacterial RNA. To demonstrate this concept, we used voltammetry in connection with pencil electrodes to detect isolated E. coli 16S rRNA. Using this approach, 10(7) cells of E. coli were detected in a quantitative, reproducible fashion in 4h. Detection was achieved without a nucleic acid amplification step. The specificity of the assay for coliforms was demonstrated by testing against a panel of bacterial RNA. We also show that E. coli RNA can be detected directly from cell extracts. The method could be used for on-site detection and shows promise for adaptation into automated biosensors for water-quality monitoring.     

Monitoring of regional drug abuse through wastewater-based epidemiology—A critical review

Wastewater-based epidemiology is a new approach to monitor drug abuse. It involves collecting wastewater, analysis of residues of drugs or its metabolites in wastewater, and back-calculation of drug consumption by taking into account wastewater flow, stability of drug target residues in wastewater, and excretion rates of drugs/metabolites. Wastewater-based epidemiology has the advantages of being inexpensive and yielding more consistent and near real-time results. It has the great potential to supplement the existing drug monitoring methods. It can be used to build large-scale (regional, national, or even continental) monitoring networks that would yield spatial patterns and temporal trends in drug abuse. This paper described in detail the principle and procedures of this wastewater-based approach. Application of this approach across the globe was also reviewed. The uncertainties involved in the approach and knowledge gaps were identified. Finally, necessity, benefits, and feasibility to set up nation or province-wide monitoring networks based on wastewater analysis in China were discussed.     

Kurzzeit-Toxizitätstests zur Messung der Toxizität von Chemikalien gegenüber Belebtschlamm

Short-term Toxicity Tests for the Measurement of Toxicity of Chemicals Towards Activated Sludge. To avoid severe damage to the activated sludge of a wastewater treatment plant it is necessary to control the sludge activity by means of biological test systems. Especially the short-term respiration assay and the dehydrogenase assay with resazurin as a redox-dye seem to be appropriate for this monitoring program. The practical performance of both test systems was optimized in some essential points. With the help of several reference substances both monitoring systems were tested for practical use as short-term toxicity tests. The results of the investigations showed that the inhibitory concentrations measured in both test systems were partially very different. In monitoring experiments with laboratory-scale wastewater treatment plants it could be shown that a positive correlation between respiration activity and dehydrogenase activity is not always given. Furthermore, a pH-dependent toxicity could be demonstrated for several substituted phenols.     

A Comprehensive Review on Microalgae-Based Biorefinery as Two-Way Source of Wastewater Treatment and Bioresource Recovery

In the past few years, microalgae have gained huge recognition from the scientific community due to their potential applications in the production of a broad array of bio-based products varying from biofuels to nanoparticles. Due to their elevated growth rate, high tolerance to various types of abiotic stresses, and complex metabolic capacity, microalgae can be used as promising tools for the attainment of a circular bioeconomy. Moreover, they can simply utilize nutrients from wastewater for biorefinery purposes, resulting in resource recovery coupled with wastewater treatment. However, due to their sub-optimal yields and high production costs, microalgae-based bio-products have not yet been commercialized. This review provides insights into the employment of microalgae as an efficient bioresource for the treatment of wastewater with simultaneous enactment as a biorefinery to produce biofuels, biochar, bioplastic, fertilizers, and other high-value bioproducts. Furthermore, the application of microalgal nanoparticles in wastewater treatment and prospects for genetic modification of microalgae for enhanced biorefinery capabilities have also been briefly highlighted.     

Restoration of the eutrophic Orbetello lagoon (Tyrrhenian Sea, Italy): water quality management

The Orbetello lagoon (Tyrrhenian coast, Italy) receives treated urban and land based fishfarms wastewater. The development of severe eutrophication imposed the three main activity adoption focuses on (1) macroalgae harvesting; (2) pumping of water from the sea; (3) confining wastewater to phytotreatment ponds. The responses to these interventions were rapid and macroalgal reduction growth and seagrass return were recorded. Since 1999, a new macroalgal development was recorded. The aim of this research was to discover whether the recent macroalgal growth can be attributed to the continuing wastewater influx from the remaining persistent anthropic sources (PAS) or from the sediment nutrient release. A monitoring programme was carried out between August 1999 and July 2000 in order to measure dissolved inorganic nitrogen and phosphorus in the wastewaters entering into the lagoon and in central lagoon areas, seaweed and seagrass distribution and lagoon N, P annual budgets. The results showed higher N and P values close to PAS. The distribution of the macroalgal species confirms that the available P comes almost entirely from these remaining PAS. In conclusion, the environmental measures adopted produced a significant reduction in algal biomass development in the lagoon; the macroalgal harvesting activities produced a sediment disturbance with following oxidize conditions, which make P unavailable in the lagoon water, excepting close the PAS.     

Early experiences in monitoring the effects of Hong Kong's new generation of sewage outfalls on the marine environment

The rapid post war development of Hong Kong as a centre of population, industry and commerce far outstripped the provision of sewers, wastewater treatment and disposal facilities. A legacy of marine pollution remains; the Government is now addressing this problem through the implementation of an ambitious sewage strategy. At the core of the strategy lies a major sewerage programme and the construction of new sewage treatment plants and marine disposal outfalls. The authors outline the background to recent sewerage and related infrastructure development in Hong Kong and discuss the scope and role of an outfall performance verification monitoring programme. This monitoring programme also is intended to identify the need for future improvements to the system in order to ensure that statutory Water Quality Objectives can be achieved and maintained. This work is seen as an essential element in the strategy for providing a cost effective treatment and disposal system. The early stages of a programme to measure the effects of major sewage outfalls on coastal waters is described. Initial work in the north-west New Territories of Hong Kong has focused on understanding the dilution and dispersion of sewage plumes. Ongoing and recently completed plume tracking work, which has made use of innovative tools such as radio-isotope tracers, dye fluorescence, ADCP and other water quality sensors multiplexed to three dimensional positional recording systems is reviewed. The subsequent interpretation of these results and their value in assessing outfall performance is considered. The success of this work is discussed and the lessons for future monitoring programmes are identified.     

Coastal pollution hazards in southern California observed by SAR imagery: stormwater plumes, wastewater plumes, and natural hydrocarbon seeps

Stormwater runoff plumes, municipal wastewater plumes, and natural hydrocarbon seeps are important pollution hazards for the heavily populated Southern California Bight (SCB). Due to their small size, dynamic and episodic nature, these hazards are difficult to sample adequately using traditional in situ oceanographic methods. Complex coastal circulation and persistent cloud cover can further complicate detection and monitoring of these hazards. We use imagery from space-borne synthetic aperture radar (SAR), complemented by field measurements, to examine these hazards in the SCB. The hazards are detectable in SAR imagery because they deposit surfactants on the sea surface, smoothing capillary and small gravity waves to produce areas of reduced backscatter compared with the surrounding ocean. We suggest that high-resolution SAR, which obtains useful data regardless of darkness or cloud cover, could be an important observational tool for assessment and monitoring of coastal marine pollution hazards in the SCB and other urbanized coastal regions.     

基于萤石云API的测震台站视频监视系统

为了提高测震台站运维工作效率,设计一套视频监视系统,实现地震台网中心对测震台站的远程、实时视频监视,数据流监视与中断告警,入侵侦测与告警以及市电中断告警等功能。系统分为台站端子系统和中心端子系统,其中,台站端子系统集成萤石网络摄像机、市电监控装置和4G路由器等设备,可以将台站视频截图通过4G信道近实时传输至中心端子系统;中心端子系统是一套应用程序,基于萤石云API开发。该系统为无人值守台站的视频监视提供了完整解决方案,在承德中心台的应用证明,可提高台网运维工作效率。     

Growing Duckweed to Recover Nutrients from Wastewaters and for Production of Fuel Ethanol and Animal Feed

Lemnaceae or duckweed is an aquatic plant that can be used to recover nutrients from wastewaters. The grown duckweed can be a good resource of proteins and starch, and utilized for the production of value‐added products such as animal feed and fuel ethanol. In the last eleven years we have been working on growing duckweed on anaerobically treated swine wastewater and utilizing the duckweed for fuel ethanol production. Duckweed strains that grew well on the swine wastewater were screened in laboratory and greenhouse experiments. The selected duckweed strains were then tested for nutrient recovery under laboratory and field conditions. The rates of nitrogen and phosphorus uptake by the duckweed growing in the laboratory and field systems were determined in the study. The mechanisms of nutrient uptake by the duckweed and the growth of duckweed in a nutrient‐limited environment have been studied. When there are nutrients (N and P) available in the wastewater, duckweed takes the nutrients from the wastewater to support its growth and to store the nutrients in its tissue. When the N and P are completely removed from the wastewater, duckweed can use its internally stored nutrients to keep its growth for a significant period of time. A modified Monod model has been developed to describe nitrogen transport in a duckweed‐covered pond for nutrient recovery from anaerobically treated swine wastewater. Nutrient reserve in the duckweed biomass has been found the key to the kinetics of duckweed growth. Utilization of duckweed for value‐added products has a good potential. Using duckweed to feed animals, poultry, and fish has been extensively studied with promising results. Duckweed is also an alternative starch source for fuel ethanol production. Spirodela polyrrhiza grown on anaerobically treated swine wastewater was found to have a starch content of 45.8% (dry weight). Enzymatic hydrolysis of the duckweed biomass with amylases yielded a hydrolysate with a reducing sugar content corresponding to 50.9% of the original dry duckweed biomass. Fermentation of the hydrolysate using yeast gave an ethanol yield of 25.8% of the original dry duckweed biomass. These results indicate that the duckweed biomass can produce significant quantities of starch that can be readily converted into ethanol.     

Developing a long-term monitoring network under uncertain flowpaths

A long-term monitoring well network is developed using complementary and simple approaches in conjunction with a stochastic ground water flow and transport model. The development is illustrated for a case study of a U.S. nuclear testing site (Shoal) that is undergoing environmental restoration. The network design builds on three different, yet complementary, tools for locating the monitoring wells with a main objective of detection monitoring. The first tool is applied to select potential siting horizons where monitoring wells could be located. The second tool is used to place monitoring wells in locations with high success probability. The success here is defined as the detection of migrating stochastic plumes before a certain mass percentage reaches a compliance boundary. The third tool is used to analyze detection efficiency of multiple combinations of three wells. Seventy-six different three-well networks are selected from 20 candidate locations and are evaluated for detection efficiency. From the 76 networks analyzed, 28 attain detection efficiency close to or above 70%. The results of the different analyses provide multiple alternatives for the locations of the three wells, which will become part of the long-term monitoring network at Shoal. A number of combinations are equally good, and the final choice will depend on practical considerations and future agreements between model sponsor and regulators.     

Development of a SPME-GC Method for the Determination of Organic Compounds in Wastewater

A manual SPME method is presented for the analysis of organic compounds in industrial wastewater. 24 compounds commonly found in the wastewater of a chemical plant in northern Germany have been selected as reference compounds. Precision, linearity, and detection limits have been determined. Moreover, the effect of methanol content, pH value, salt content, and an excess of compounds on the extraction process have been studied. Several compounds have been investigated for their applicability as internal standards to quantify the compounds of interest. Since the method will later be transferred to a fully automated SPME-GC system, which will be operated directly on-site at an industrial wastewater purification plant, special attention has been paid to the analysis of real wastewater samples. In this context, the fibre stability has been studied by performing 53 extraction/desorption cycles from one wastewater sample. Neither a decrease in fibre performance nor in precision has been observed indicating that the proposed method is suitable for the analysis of real wastewater.     

铁磁形状记忆合金研究进展与展望（Ⅲ）：应用研究

阐述了铁磁形状记忆合金（Ferromagnetic Shape Memory Alloy,简写为FSMA）在智能材料及其应用领域的优越性。重点介绍了FSMA装置研发和基于FSMA装置的体系分析理论的发展历程和研究现状。仔细分析了各种FSMA装置频率、输出力和最大位移等关键技术指标,讨论了基于电磁学、热力学和力学原理的包含FSMA装置体系的分析理论,指出了各自在面向应用时存在的问题。研究表明,FSMA独特的性能使其具有广阔的应用前景,然而在装置研发和系统理论领域还有待深入。最后指出,更广领域的新型FSMA装置和基于FSMA装置的体系分析理论等问题还有待进一步研究。     

Algal Cultivation for Treating Wastewater in African Developing Countries: A Review

The tremendous increase in human population and rapid decline in freshwater resources have necessitated the development of innovative and sustainable wastewater treatment methods. Africa as a developing continent is currently backing on sustainable solutions to tackle impending water resource crisis brought forward by wastewater‐induced environmental pollution and climate change. Microalgae‐based wastewater treatment systems represent an emerging technology that is capable of meeting the new demand for improved wastewater treatment and climate change mitigation strategies in an environmentally friendly manner. This review critically looks at the opportunities of Africa in harnessing and exploiting the potential of microalgae for the treatment of various wastewaters based on their capacity to recycle nutrients and for concurrent production of valuable biomass and several useful metabolites. Wastewaters, if improperly/completely untreated and discharged, simultaneously pollute freshwater sources and present significant health and environmental risks. Nutrients in wastewater can be utilized and recovered in the form of marketable biomass and products when integrated with the cultivation of microalgae. Several valuable bioproducts can be generated from wastewater‐grown microalgal biomass including biofuels, biofertilizers, animal feed, and various bioactive compounds. This biorefinery approach would most certainly improve wastewater treatment process economics, enhancing the technical feasibility of algae‐based wastewater remediation in African countries.     

Local groundwater balance model: stakeholders’ efforts to address groundwater monitoring and literacy

Understanding groundwater systems is crucial to ensure their monitoring and protection. Global groundwater models and predictive tools exist to better understand them. In view of scarce groundwater data, especially in developing countries, the question of how to model these systems and make them usable for groundwater management is crucial. Herein, we demonstrate how a transdisciplinary process can overcome the data scarcity problem and lead to the development of a local groundwater balance model (LGBM). Over 50 actors from more than 15 disciplines and groundwater-related sectors were involved for the case of Yucatan, Mexico. Results revealed high wastewater emissions to the aquifer discharged without treatment and poor recycling practices. The method can be adapted to specific regions, can be used to address methodological challenges for monitoring, and can contribute to the achievement of the 2030 Development Agenda by addressing Sustainable Development Goal 6-related Targets (6.3, 6.4, 6.5, 6.6(a, b)).     

Municipal Wastewater Treatment Using Moringa oleifera Seed and Press Cake Powder: A Comparative Analysis

Moringa oleifera (MO), also known as drumstick tree, has gained worldwide attention among researchers and policy makers, for showing its remarkable potential in environmental management. Recently, MO seed or press cake (a by-product of oil extraction industry) powder has also emerged as an alternative and promising coagulant for environmental remediation. In this view, attempts have been made in this study to evaluate the efficacy of MO seed and press cake powder (MSP & PCP) for the treatment of municipal wastewater. Both MSP and PCP are characterized by using swelling ratio, scanning and transmission electron microscopy analysis. The effect of various operational parameters such as coagulation–flocculation pH (2–10), coagulant dosage (0.1–1 g L⁻¹), and sedimentation time (0–180 min), etc., is investigated to understand the potential of MSP and PCP. At optimized operational conditions, sedimentation kinetics is also performed suggesting that the treatment process is being governed by the second-order kinetic model. The chemical and biological oxygen demand removals at optimized conditions are observed as follows: ≈38 and ≈73% for MSP and ≈47 and ≈85% for PCP. Overall, the results of the present study elucidated that PCP can be employed as a promising alternative of MSP for municipal wastewater with improved treatment efficiencies.     

基于区域生长法和变差函数的PolSAR影像山脊线提取

山脊线通常是地震引发的山地灾害的起始部位。在监测此类山地灾害时,需要明确山脊线所处位置,从而为制定出相关预防措施提供支持。根据实际经验,由于合成孔径雷达(Synthetic Aperture Radar,SAR)的成像特殊性,在检测含建筑物的全极化SAR(Polarimetric Synthetic Aperture Radar,PolSAR)影像山脊线时,往往受到高散射强度的建筑物干扰,从而出现误判。针对PolSAR影像中山脊线受到建筑物干扰出现误识的问题,提出一种结合区域生长法和变差函数的识别方法。在识别过程中,首先,对采集的PolSAR影像通过区域生长法进行分割处理,再通过模糊C均值(Fuzzy C-Means,FCM)分类方法对变差纹理等特征进行聚类分析,并进行相似度判断,获取建筑物区域,在此基础上,对比建筑物、山脊线的混分成分,进而提取山脊线。通过对该方法进行实验对比分析,发现其相较于阈值分割方法提取精度有明显提高,可以为PolSAR影像中山脊线识别提供一种新思路。     

大震震中参数及时测定在救灾中的意义

从测震学的角度,审视了大震参数快速测定的方法,讨论了为保证测定参数所需能完整记录大地震的仪器配套问题。叙述了适用于城市救灾的高技术救灾器械的研制问题。     

Innovative Sampling Techniques for Ground Water Monitoring at Hazardous Waste Sites

The authors have recently used several innovative sampling techniques for ground water monitoring at hazardous waste sites. Two of these techniques were used for the first time on the Biscayne Aquifer Super-fund Project in Miami, Florida. This is the largest sampling program conducted so far under the U.S. Environmental Protection Agency (EPA) Superfund Program.
One sampling technique involved the use of the new ISCO Model 2600 submersible portable well sampling pump. A compressed air source forces water from the well into the pump casing and then delivers it to the surface (through a pulsating action). This pump was used in wells that could not be sampled with surface lift devices.
Another sampling technique involved the use of a Teflon manifold sampling device. The manifold is inserted into the top of the sampling bottle and a peristaltic pump creates a vacuum to draw the water sample from the well into the bottle. The major advantage of using this sampling technique for ground water monitoring at hazardous waste sites is the direct delivery of the water sample into the collection container. In this manner, the potential for contamination is reduced because, prior to delivery to the sample container, the sample contacts only the Teflon, which is well-known for its inert properties.
Quality assurance results from the Superfund project indicate that these sampling techniques are successful in reducing cross-contamination between monitoring wells. Analysis of field blanks using organic-free water in contact with these sampling devices did not show any concentration at or above the method detection limit for each priority pollutant.     

核电站结构隔震研究进展

基底隔震已经大量应用于一般民用建筑中,被认为是在剧烈地震中保护建筑物最具发展前景的技术之一,但对于核电站这类特殊的建筑物应用较少,且研究比较薄弱。介绍了核电站水平及三维隔震装置的研发及性能测试,概述了核电站隔震系统抗震性能的研究进展和核电站隔震设计规范,指出了在核电站中应用隔震技术还有待解决的问题,对今后核电站隔震的研究提出了一些建议。     

Hydraulic Tomography Offers Improved Imaging of Heterogeneity in Fractured Rocks

Fractured rocks have presented formidable challenges for accurately predicting groundwater flow and contaminant transport. This is mainly due to our difficulty in mapping the fracture‐rock matrix system, their hydraulic properties and connectivity at resolutions that are meaningful for groundwater modeling. Over the last several decades, considerable effort has gone into creating maps of subsurface heterogeneity in hydraulic conductivity (K) and specific storage (S_s) of fractured rocks. Developed methods include kriging, stochastic simulation, stochastic inverse modeling, and hydraulic tomography. In this article, I review the evolution of various heterogeneity mapping approaches and contend that hydraulic tomography, a recently developed aquifer characterization technique for unconsolidated deposits, is also a promising approach in yielding robust maps (or tomograms) of K and S_s heterogeneity for fractured rocks. While hydraulic tomography has recently been shown to be a robust technique, the resolution of the K and S_s tomograms mainly depends on the density of pumping and monitoring locations and the quality of data. The resolution will be improved through the development of new devices for higher density monitoring of pressure responses at discrete intervals in boreholes and potentially through the integration of other data from single‐hole tests, borehole flowmeter profiling, and tracer tests. Other data from temperature and geophysical surveys as well as geological investigations may improve the accuracy of the maps, but more research is needed. Technological advances will undoubtedly lead to more accurate maps. However, more effort should go into evaluating these maps so that one can gain more confidence in their reliability.