Effect of substrate grain size on the growth and morphology of the submersed macrophyte Vallisneria natans L.

Experiments were conducted to investigate the growth and morphology of the submersed macrophyte Vallisneria natans in relation to seven substrate types. The study also aims to evaluate the influence of the grain size of the aquatic substrate on the submersed macrophyte. The results show that growth, morphology and biomass accumulation of V. natans were significantly affected by the physical properties of the growth substrate. Compared with V. natans grown in pebble and gravel substrates, V. natans grown in silt and clay substrates had greater height, more ramets and leaves, as well as greater biomass accumulation. No significant differences in biomass allocation patterns were found among the different experimental treatments. The total porosity and dry bulk density of the growth substrate strongly influence plant traits. Substantial positive correlations were observed between total porosity and height and total biomass. Also, negative correlations were observed between dry bulk density and height, number of ramets, total biomass and lacunal volume. These results indicate that at high concentrations of water-column nutrients, V. natans responds to different physical properties of the growth substrate by changing growth and biomass accumulation strategies, rather than by changing biomass allocation patterns. Compared with V. natans grown in substrates with larger grain sizes, V. natans appeared well-adapted to substrates with smaller grain sizes. Thus, the physical properties of the growth substrate could be important in determining submersed macrophyte colonization and distribution in shallow eutrophic lakes. This information may be useful in managing shallow eutrophic lakes wherein rooted submersed macrophytes are declining.     

On the period and quality factor of the Chandler wobble

The motion of the Earth’s geophysical pole is modeled by numerical integration of the Liouville equations. The dependence of variable parameters of these equations (the period of free nutation T and the quality factor of the shell Q) is examined as a function of time and initial data sets used for constructing geophysical perturbing functions. In particular, we used oceanic perturbing functions constructed from TOPEX/POSEIDON altimetry data. The annual and Chandler components of the pole motion were also modeled. Apart from the subtraction of quadratic trends, initial data were not filtered or averaged. The performed analysis provided model values of T = 425–440 days and Q = 20–60, and Q was found to be somewhat unstable with time.     

Effects of hypoxia in the gills of the Manila clam Ruditapes philippinarum using NMR-based metabolomics

Coastal hypoxia affects the survival, behavior, and reproduction of individual local marine organisms, and the abundance, biomass, and biodiversity of coastal ecosystems. In this study, we investigated the chronic effects of hypoxia on the metabolomics in the gills of Ruditapes (R.) philippinarum. The results indicated significant alterations in the metabolite profiles in the gills of the hypoxia-treated clams, in comparison with those maintained under normoxia. The levels of betaine, taurine, glycine, isoleucine, and alanine were significantly reduced, suggesting a disturbance of osmotic balance associated with hypoxia. Meanwhile, metabolites involved in energy metabolism, such as alanine and succinate, were also affected. Dramatic histopathological changes were observed in the gills and hepatopancreases of R. philippinarum grown in hypoxic waters, demonstrating tissue damages apparently caused by long-term exposure to hypoxia. Our findings suggest that hypoxia significantly affects the physiology of R. philippinarum, even at a sub-lethal level, and impedes health of the clams.     

Microbial water quality of the Georgian coastal zone of the Black Sea

From 2006 to 2008, microbial water quality was monitored along the Georgian coast of the Black Sea. Temperature, pH, salinity, and dissolved oxygen were measured, along with a variety of aquatic microbial parameters, including heterotrophic plate count (HPC), total culturable bacterial count (TCBC), and chlorophyll a (Chl-a) concentration. Total and fecal coliforms and total enterococci counts were recorded as indicators of fecal pollution. Vibrio bacteria, and Escherichia coli- and Vibrio-specific bacteriophages were isolated and enumerated to determine their relationships to standard marine pollution indicators.Persistent microbial pollution was observed, particularly in the summer months, with a higher rate of contamination in estuaries. Microbial indicators generally showed seasonal dependence, suggesting that temperature may influence bacterial dynamics in this environment. No correlation was apparent between fecal pollution indicators and physical-chemical and aquatic microbial parameters, although there were significant relationships amongst the indicators themselves, as well as with the prevalence of Vibrio bacteria and phage.     

Modelling the Velocity Field in a Regular Grid in the Area of Poland on the Basis of the Velocities of European Permanent Stations

The paper presents the results of testing the various methods of permanent stations’ velocity residua interpolation in a regular grid, which constitutes a continuous model of the velocity field in the territory of Poland. Three packages of software were used in the research from the point of view of interpolation: GMT (The Generic Mapping Tools), Surfer and ArcGIS. The following methods were tested in the softwares: the Nearest Neighbor, Triangulation (TIN), Spline Interpolation, Surface, Inverse Distance to a Power, Minimum Curvature and Kriging. The presented research used the absolute velocities’ values expressed in the ITRF2005 reference frame and the intraplate velocities related to the NUVEL model of over 300 permanent reference stations of the EPN and ASG-EUPOS networks covering the area of Europe. Interpolation for the area of Poland was done using data from the whole area of Europe to make the results at the borders of the interpolation area reliable. As a result of this research, an optimum method of such data interpolation was developed. All the mentioned methods were tested for being local or global, for the possibility to compute errors of the interpolated values, for explicitness and fidelity of the interpolation functions or the smoothing mode. In the authors’ opinion, the best data interpolation method is Kriging with the linear semivariogram model run in the Surfer programme because it allows for the computation of errors in the interpolated values and it is a global method (it distorts the results in the least way). Alternately, it is acceptable to use the Minimum Curvature method. Empirical analysis of the interpolation results obtained by means of the two methods showed that the results are identical. The tests were conducted using the intraplate velocities of the European sites. Statistics in the form of computing the minimum, maximum and mean values of the interpolated North and East components of the velocity residuum were prepared for all the tested methods, and each of the resulting continuous velocity fields was visualized by means of the GMT programme. The interpolated components of the velocities and their residua are presented in the form of tables and bar diagrams.     

Zooplankton and Karenia brevis in the Gulf of Mexico

Blooms of the toxic dinoflagellate Karenia brevis are common in the Gulf of Mexico, yet no in situ studies of zooplankton and K. brevis have been conducted there. Zooplankton abundance and taxonomic composition at non-bloom and K. brevis bloom stations within the Ecology of Harmful Algal Blooms (ECOHAB) study area were compared. At non-bloom stations, the most abundant species of zooplankton were Parvocalanus crassirostris, Oithona colcarva, and Paracalanus quasimodo at the 5-m isobath and P. quasimodo, O. colcarva, and Oikopleura dioica at the 25-m isobath. There was considerable overlap in dominance of zooplankton species between the 5 and 25-m isobaths, with nine species contributing to 90% of abundance at both isobaths. At stations within K. brevis blooms however, Acartia tonsa, Centropages velificatus, Temora turbinata, Evadne tergestina, O. colcarva, O. dioica, and P. crassirostris were dominant. Variations in abundance between non-bloom and bloom assemblages were evident, including the reduction in abundance of three key species within K. brevis blooms.     

Distribution of hydrocarbons in the oyster,Pinctada margaratifera,along the coast of Kuwait

Concentrations of total hydrocarbons within the boiling point range of the alkanes n-C₁₄ and n-C₃₂ were determined in oysters, Pinctada margaratifera, from coastal waters of Kuwait. Levels of petroleum-derived hydrocarbons were highest in an area adjacent to the major oil loading facilities. Whether the use of dispersants to treat minor spills increases levels of incorporation of petroleum compounds into the food webs could not be concluded from the data of this study. Levels of total petroleum-type hydrocarbons in the oysters at this site were equivalent to those in mussels, Mytilus sp., from harbours, bays and urban coastal areas of California. The Kuwaiti oysters lacked a C₂₈ pentacyclic triterpane that was present in extracts of mussels from southern California that had been recently exposed to a minor spill or to a natural seepage. Levels of DDE and PCB were comparable to those in relatively unpolluted areas of North America.     

On the remote monitoring of Karenia brevis blooms of the west Florida shelf

In situ surveys (1997–2002) of Karenia brevis distribution on the west Florida shelf were used to explain spectral remote sensing reflectance, chlorophyll-a concentration, and backscattering coefficient estimates derived using SeaWiFS satellite data. Two existing approaches were tested in an attempt to differentiate K. brevis blooms from other blooms or plumes. A chlorophyll-anomaly method used operationally by the National Oceanic and Atmospheric Administration (NOAA) sometimes correctly identified K. brevis blooms but also generated false positives and false negatives. The method identified approximately 1000 km² of high chlorophyll-anomalies (>1 mg m⁻³) off southwest Florida between the 10 and 50-m isobaths nearly every day from summer to late fall. Whether these patches were K. brevis blooms or not is unknown. A second method used a backscattering:chlorophyll-a ratio to identify K. brevis patches. This method separated K. brevis from other blooms using in situ optical data, but it yielded less satisfactory results with SeaWiFS data. Spectral reflectance (R_rs) estimates for K. brevis blooms, diatom blooms, and coastal river plumes are statistically similar for many cases. Large pixel size, shallow water, and imperfect algorithms distort satellite retrievals of bio-optical parameters in patchy blooms. At present, a combination of chlorophyll-a, chlorophyll-anomaly, backscattering:chlorophyll-a ratio, RGB composites, MODIS fluorescence data, as well as time-series analysis and ancillary data such as winds, currents, and sea surface temperature can improve K. brevis bloom assessments. Progress in atmospheric correction and bio-optical inversion algorithms is required to help improve capabilities to monitor K. brevis blooms from space. Further, satellite sensors with improved radiometric capabilities and temporal/spatial resolutions are also required.     

Anomalous variations in the foF2 critical frequency above Japan prior to the earthquake of March 9, 2011

Based on data from the Japanese Kokubunji and Wakkanai stations of vertical sounding of the ionosphere, the variations in the foF2 critical frequency prior to the strong earthquakes of March 9 and 11, 2011 (M 7.2 and 9.0, respectively), are analyzed. It is found that significant positive disturbances of foF2 had been recorded approximately one day before the first earthquake. Notably, at the Irkutsk reference station, which is located about 3300 km from the earthquake epicenters, there were no significant disturbances of foF2. This suggests that the effects of increased foF2, observed at the Kokubunji and Wakkanai stations, were probably caused by the earthquake preparation processes. The seismo-ionospheric manifestations of the stronger earthquake on March 11, 2011, even if they took place, were hidden by the geomagnetic storm’s effects.     

Effects of the modified Huanglian Jiedu decoction on the disease resistance in grey mullet (Mugil cephalus) to Lactococcus garvieae

Lactococcosis is prevalent on grey mullet (Mugil cephalus) in Hong Kong aquaculture resulting in serious economic loss. A compound formulation of Traditional Chinese Medicines (TCM) (modified Huanglian Jiedu decoction (HLJDD)) comprising Rhizoma coptidis, Radix scutellaria, Cortex phellodendri, Fructus gardeniae, Fructus forsythiae and Flos lonicerae japonicae (in a ratio of 3:2:2:3:3:5) were applied as feed supplements to deal with the disease. The Nitroblue tetrazolium activity in blood, bactericidal activity and total immunoglobulin in plasma were significantly enhanced after feeding 1% of this TCM for 28 days. The disease resistances to Lactococcus garvieae in 1% and 2% TCM feeding groups were significantly enhanced. In the in vitro study, the modified HLJDD also activated the plasma bactericidal activities (p < 0.01). Based on this study, 1% modified HLJDD feeding for 28 days may be an optimal dose to prevent L. garvieae infection and could be used in aquaculture industries.     

Earthquake responses in the high-latitude ionosphere

The data, obtained using the methods of partial reflections and ionosphere vertical sounding on the Kola Peninsula and in Scandinavia, at Tumannyi (69.0° N, 35.7° E) and Sodankyla (67.37°N, 26.63°E) observatories, have been analyzed in order to detect earthquake responses. The strong earthquakes have been considered: one earthquake with a magnitude of 7.7 occurred at 0819:25 UT on July 17, 2006, on the western coast of Indonesia (9.33° S, 107.26° E), and another earthquake with a magnitude of 6.2 occurred 2253:59 UT on May 26, 2006, on Yava (7.94° S, 110.32° E). These earthquakes, the epicenters of which were located in the same region and at identical depths (10 km), were observed under quiet conditions in the geomagnetic field (ΣK _p = 5.7 and 6.3) and during small solar flares. The response of the ionosphere to these flares was mainly observed in the parameters of the lower ionosphere in the D and E regions. It has been found out that the period of variations in the ordinary component of the partially reflected signal at altitudes of the E region increased before the earthquake that occurred on July 17, 2006. The f _min variations at Sodankyla observatory started 20 h before the earthquake. The periods of these variations were 3–6 h. The same periods were found in the variations in other ionospheric parameters (foEs and h’Es). The variations in the ordinary component of partially reflected signals with periods of 2–5 hours were observed on the day of another earthquake (May 26, 2006). Internal gravity waves with periods of several hours, which can be related to the earthquakes, were detected in the amplitude spectra of the ordinary component of partially reflected signals and in other parameters in the lower ionosphere.     

What factors drive seasonal variation of phytoplankton,protozoans and metazoans on leaves of Posidonia oceanica and in the water column along the coast of the Kerkennah Islands,Tunisia?

A hierarchical sampling design was used during two seasons (spring (May) and summer (August) 2006). Using this design, three regions of the Kerkennah Islands (Tunisia) were analyzed for the distribution of microalgal, protozoan and metazoan assemblages in two different habitats: (1) the water column; and (2) on Posidonia oceanica (L.) Delile (P. oceanica) leaves in shallow meadows. A total of 85 species were obtained. In particular, the diatom family Naviculacea consistently dominated (both numerically and in their diversity) the micro-algae in all regions for the two seasons of the study and in both habitats. In the Chergui region, which is the closest area to a source of impact, fast growing centric diatoms (such as Thalassionema, Rhizosolenia, Striatella, and Skeletonema) were identified as indicators of high organic matter and nutrient enrichment in water bodies. Protozoan and metazoan species abundance in the different regions indicate a non-random spatial and temporal distribution of the epiphytic organisms on leaves of P. oceanica that correlated with phytoplankton. The results also indicate that (1) the abundance of micro- and macroorganisms in the three regions were higher on P. oceanica leaves than in the water column for the two seasons; (2) environmental factors such as currents and tide influenced assemblages; and (3) the highest abundance was due to direct exposure to the polluted coast of Sfax and the effect of tidal asymmetries generating nutrient-rich inputs from the city.     

Retrospective Study on the Predictability of Pattern Informatics to the Wenchuan M8.0 and Yutian M7.3 Earthquakes

Two large earthquakes occurred in the western part of China in 2008, one of them being the Yutian (35.6°N, 81.6°E) M7.3 earthquake that occurred on March 21 (BJT) and the other the Wenchuan (31.0°N, 103.4°E) M8.0 earthquake that occurred on May 12 (BJT). In this paper, the West Continental China (included in 20.0°–50.0°N, 70.0°–110.0°E region) was the study region for verifyong the predictability of the pattern informatics (PI) method using the receiver-operating characteristic curve (ROC) test and R score test. Different forecasting maps with different calculating parameters were obtained. The calculating parameters were the grid size Δx, base time t _b, reference interval t _b to t ₁, change interval t ₁ to t ₂, and forecasting interval t ₂ to t ₃. In this paper, the base time t _b fixed to June 1, 1971, the ending forecast time t ₃ fixed to June 1, 2008, and the forecasting interval t ₂ to t ₃ changed from 1 to 10 years, and the grid sizes were chosen as 1° × 1° and 2° × 2°, respectively. The results show that the PI method could forecast the Yutian M7.3 and Wenchuan M8.0 earthquakes only using suitable parameters. Comparing the forecast results of grid sizes 1° × 1° and 2° × 2°, the models with 2° × 2° grids were better. Comparing the forecast results with different forecasting windows from 1 to 10 years, the models with forecasting windows of 4–8 years were better using the ROC test, and the models with forecasting windows of 7–10 years were better using the R score test. The forecast efficiency of the model with a grid size of 2° × 2° and forecast window of 8 years was the best one using either the ROC test or the R score test.     

Ballast water as a vector of coral pathogens in the Gulf of Mexico: the case of the Cayo Arcas coral reef

The discharge of nutrients, phytoplankton and pathogenic bacteria through ballast water may threaten the Cayo Arcas reef system. To assess this threat, the quality of ballast water and presence of coral reef pathogenic bacteria in 30 oil tankers loaded at the PEMEX Cayo Arcas crude oil terminal were determined. The water transported in the ships originated from coastal, oceanic or riverine regions. Statistical associations among quality parameters and bacteria were tested using redundancy analysis (RDA). In contrast with coastal or oceanic water, the riverine water had high concentrations of coliforms, including Vibrio cholerae 01 and, Serratia marcescens and Sphingomona spp., which are frequently associated with “white pox” and “white plague type II” coral diseases. There were also high nutrient concentrations and low water quality index values (WQI and TRIX). The presence of V. cholerae 01 highlights the need for testing ballast water coming from endemic regions into Mexican ports.     

Long-term earthquake prediction in the Marmara region based on the regional time- and magnitude-predictable model

In order to estimate the recurrence intervals for large earthquakes that occurred in the Marmara region, this region, limited with the coordinates of 39°–42°N, 25°–32°E, has been separated into seven seismogenic sources on the basis of certain seismological criteria, and regional time- and magnitude-predictable model has been applied for these sources. Considering the interevent time between successive mainshocks, the following two predictive relations were computed: log T _t = 0.26 M _min + 0.06 M _p –0.56 log M ₀ + 13.79 and M _f = 0.63 M _min ? 0.07 M _p + 0.43 log M ₀ ? 7.56. Multiple correlation coefficient and standard deviation have been computed as 0.53 and 0.35 for the first relation and 0.66 and 0.39 for the second relation, respectively. On the basis of these relations and using the occurrence time and magnitude of the last mainshocks in each seismogenic source, the probabilities of occurrence P(Δt) of the next mainshocks during the next five decades and the magnitude of the expected mainshocks were determined.     

Characteristics of seismic wave attenuation in the crust and upper mantle of the Northern Caucasus

The quality factor Q as a function of frequency in an S wave range of 1–8 Hz is estimated from records of ～60 earthquakes (M _w > 3.9 and source depths of 1–60 km) obtained at the Sochi seismic station at epicentral distances of less than ～300 km. Methods of Q estimation used in the paper were developed in works by Aki, Rautian, and others; they are based on the suppression of source-related and local effects in S wave spectra with the help of coda waves measured at a fixed time from the first arrival. To compensate for directivity effects, averaging was performed over the set of events whose sources were located in a wide range of back azimuths. The geometric divergence is represented as a three-segment function: 1/R, 1, and 1/√R at epicentral distances of 1/50–1/70 to 50–70 km, 50–70 to 130–150 km, and greater than 130–150 km, respectively. The geometric divergences in this model yielded the following estimates of the quality factor: Q(f) ～ 80f ^0.9 with a base of 35–280 km and Q(f) ～ 110f ^0.8 with a base of 60–280 km. The resulting combinations of the propagation path effects (Q and the geometric divergence) can be used for predicting strong motion parameters in the Northern Caucasus.     

Radiolarian biostratigraphy in the Southern Bering Sea since Pliocene

Detailed radiolarian biostratigraphy in the Plio-Pleistocene was analyzed by using samples from IODP Site U1340 that was drilled to a core depth of 604 m in the southern Bering Sea. A total of 227 species belonging to 102 genera were identified. Based on the distributions of the radiolarian index species at Site U1340, five radiolarian zones since the Pliocene were established in the southern Bering Sea for the first time, and 25 radiolarian bioevents were recognized. Their ages were estimated on the basis of the age-depth plot that was constructed by the synthetical datum of the effective biostratigraphic and magnetostratigraphic events. The radiolarian zones at Site U1340 were systematically compared with those in its adjacent regions since the late Early Pliocene, which further improved and interpreted the biostratigraphic datum as well as their correlations in the middle-high latitude of the North Pacific. In addition, the comparative results of radiolarian zones show that Botryostrobus aquilonaris Zone emended in this paper is equivalent to the upper part of the same zone defined by Hays, 1970, and Druppatractus irregularis-Dorydruppa bensoni Zone as well as Spongodiscus sp. Zone, newly proposed in this paper, are well correlated with Cycladophora sakaii Zone and Stylatractus universus Zone in the subarctic North Pacific, respectively.     

The factors controlling the partitioning of polybrominated diphenyl ethers and polychlorinated biphenyls in the water-column of the Pearl River Estuary in South China

In this study, the distribution and partition of polybrominateddiphenyl ethers (PBDEs) and polychlorinated biphenyls (PCBs) in water from the Pearl River Estuary were investigated. A seasonal dependence for dissolved and particle phase PCB and PBDE levels was observed. A high dissolved phase level occurred in the dry season. The positive correlation between POC content in the particles and phase distribution coefficients (K_p) suggested that POC may play an important role in determining the partition of PBDEs and PCBs. A linear correlation between the observed log K_OC and log K_OW for PBDEs and PCBs was found with slopes less than one. The observed log K_OC values were lower than the predicted log K_OC values for PBDEs (except for BDE28). The log K_OC values were higher than the predicted values for PCBs. A combination of sorption to colloids (10-98% for PBDEs and less than 25% for PCBs) and adsorption to BC was responsible for this observation.     

The relationships between particulate and soluble alkaline phosphatase activities and the concentration of phosphorus dissolved in the seawater of Toulon Bay (NW Mediterranean)

The activities of particulate and soluble phosphatase were analyzed monthly for 1 year in the coastal ecosystems of the NW Mediterranean Sea. The mean contribution of the particulate activity increased from 56% at an MUF-P concentration of 30 μM to 77% at 0.04 μM. This particulate activity was negatively correlated with the DIP, DOP and TDP concentrations when the activities were related to the seawater volume, chlorophyll a or the protein concentration. The TDP correlations were highly significant (p: 0.001). The DOP correlations were significant (p: 0.04) and became highly significant (p: 0.009) at low DIP concentrations (<0.13 μM). The DIP correlations were significant (p: 0.04) only at low DOP concentrations (<0.18 μM). Thus, the effects of seawater DIP and DOP were found to be linked. The soluble activity exhibited distinct phosphatase fractions with high (0.5–29.5 μM) and low (0.02–2 μM) Km values, but none exhibited significant correlations with phosphorus compounds.