Acute toxicity of bis(tributyltin) oxide to a marine copepod

A static renewal toxicity test was conducted to evaluate the response of the copepod Acartia tonsa to bis(tributyltin) oxide (TBTO), the active compound of recently developed antifouling paints. Copepods were individually placed in test tubes containing 4 ml of 0.3, 0.5, 1.0, 1.7 or 3.0 μg l^?1 TBTO with acetone and seawater. Thirty copepods were used for each treatment and control condition and all solutions were renewed daily. The 96-h LC₅₀ was 1.0 μg l^?1 with a 95% confidence interval between 0.8 and 1.2 μg l^?1 TBTO. The 144-h EC₅₀ was 0.4 μg l^?1 TBTO which approaches concentrations recently measured in San Diego Bay. Some copepods exposed to 0.3 μg l^?1 TBTO became moribund after six days.     

Acute toxicity of copper to sea catfish

Sea catfish (Arius felis) were exposed to aqueous solutions of reagent grade cupric chloride in artificial seawater ($\text{30.0±2.0‰}$, 21–23°C) in four static bioassays. The 24, 48, 72 and 96 h LC₅₀ were calculated and found to be 5.43, 4.17, 3.57 and 2.40 mg l.^?1 copper, respectively. Experimental concentrations of copper producing subtle behavioral changes in this species correspond to less than 0.3% of the 72 h LC₅₀. Based on this comparison with literature values, a new, maximum ‘safe’ concentration for copper in marine waters of 0.01 mg l.^?1 is proposed.     

Use of a harpacticoid copepod in toxicity tests

The harpacticoid copepod Nitocra spinipes has been tested for acute toxicity of 12 metal chlorides in brackish water. Their order of toxicity, expressed as 96 h LC₅₀, was in good agreement with other investigations performed in freshwater and seawater. The 96 h LC₅₀-values were of intermediate levels compared to these two environments. The organochlorines p,p′-DDE and p,p′-DDE methyl sulphone were tested for effects on reproduction and mortality during two weeks, and it was found that p,p′-DDE was the most toxic. It is concluded from the investigation that N. spinipes is a suitable toxicity test organism in brackish water.     

Differentiation of the sensitivity to copper and cadmium in different life stages of a copepod

Copper is more toxic to all life stages of the copepod Tisbe than cadmium. The most sensitive life stage of Tisbe to both copper and cadmium is the one-day-old nauplius. The resistance of larval stages of Tisbe increases with age (one-day-old nauplii 48h $\text{lc}{}_{50}\text{=0.3142}$ mg Cu l^?1. and 0.5384 mg Cd l^?1, 0.3415 mg Cu l^?1. and 0.645 mg Cd l^?1. for five-days-old nauplii and 0.5289 mg Cu l^?1. and 0.9061 mg Cd l^?1. for ten-days-old nauplii. The two reproductive stages of Tisbe tested (females with ovigerous bands and females bearing the first ovigerous sac) demonstrated an increased sensitivity to metals and proved more sensitive than the ten-days-old copepodids (only females with ovigerous bands had a similar sensitivity to copper with the ten-days-old copepodids).     

Some effects of sub-lethal concentrations of copper on a marine copepod

Laboratory experiments were carried out to the influence of different concentrations of copper in feeding and respiratory rates, fecundity and longevity of the marine planktonic copepod Acartia clausi, taken from a polluted and a clean area of the Saronic gulf (Greece). In the range of copper concentrations 0.001 to 0.01 mg l.^?1, all tested activities of the animals coming from the clean region seem affected. Feeding acitity, longevity and fecundity showed a progressive reduction from 0.001 to 0.01 mg l.^?1 On the other hand oxygen consumption rates showed a continuous increase at the same range of concentrations.The pollution-adapted population of Acartia seems more resistant to sub-lethal copper stress. Although longevity and respiration were affected in all concentrations used (the reaction was of the same kind as for the animals from the clean region), ingestion rate was not affected at 0.001 mg l.^?1 decreasing at higher concentrations. The fecundity of the pollution-adapted population is higher than that of the clean area, shows a slight increase at 0.001 and 0.0025 mg l.^?1 dropping about to the control animals fecundity level at 0.01 mg l.^?1     

Acute toxicity of inorganic mercury to Capitella

The polychaete worm Capitella capitata is shown to be fairly resistant to high concentrations of inorganic mercury. Survival of adults compared with those of the polychaete Ophryotrocha labronica shows that C. capitata can withstand 1 ppm Hg²⁺ for several days whilst O. labronica succombs to 0.5 ppm Hg²⁺ in 2 h.     

Acute toxicity of mine tailings to four marine species

Acute toxicity bioassays were conducted on mine tailings produced by pilot plant testing for the proposed Quartz Hill molybdenum mine, which will be situated near Ketchikan, Alaska. Tailings bioassays were conducted in seawater with juvenile coho salmon (Oncorhynchus kisutch), mussel larvae (Mytilus edulis), infaunal amphipods (Rhepoxynius abronius), and euphausiids (Euphausia pacifica). The same general range of mine tailings concentrations was acutely toxic to all four test species with acute effects observed between 61 000 to 277 000 mg l^?1 (wet wt) tailings solids (range of 95% confidence limits for LC₅₀ and EC₅₀ values). Chemical analyses of bioassay test solutions and leaching test solutions were conducted for metals (including Cd, Cu, Pb, Zn, Mn and Mo), EPA Priority Pollutant base/neutral organics, and more general parameters such as sulphate, nitrate/nitrite, cyanides, phosphate and ammonia. Parameters possibly contributing to the observed toxicity were complex contaminant mixtures including total suspended solids and heavy metals. The present study provides information related to the marine disposal of mine tailings and shows that these mine tailings present a relatively low level of acutely toxic effects.     

Acute toxicity of heavy metals to some marine larvae

The toxicity of copper, mercury and zinc to the larvae of oysters, shrimp, crab and lobsters has been examined over periods of up to 64 hours. Mercury was found to be more toxic than copper and zinc, which had similar levels of toxicity. Over the experimental period, the relationship between toxicity and concentration was linear. Larvae were from 14 to 1,000 times more susceptible than adults of the same species. The median lethal concentrations (LC₅₀) of each metal to the most sensitive species of larvae, tested over a 48 hour period, exceeded the concentrations found in natural sea water by a factor of 100. For longer test periods, the LC₅₀ would be considerably less and this factor would then be considerably reduced. Hence the continued addition of these metals to confined waters should give cause for concern.     

Acute toxicity of alkyl leads to some marine organisms

Following the wreck of the cargo ship Cavtat in the Adriatic Sea and the danger of pollution by alkyl lead antiknock compounds, the acute toxicity of alkyl leads to marine organisms at different trophic levels has been determined in the laboratory.     

Effect of chelation on toxicity of copper

Metals in the sea may form complexes with organic compounds, a process known as chelation, and this may modify their properties very greatly. Here the effect of chelation on the toxicity of copper to algae has been studied in the laboratory using the powerful chelating agent EDTA. Similar phenomena in the sea have implications both for anti-fouling paints, which usually contain copper, and for metal pollution in waters with a high organic content.     

Acute toxicity of cadmium to Channa punctatus (BLOCH)

Effects of acute cadmium poisoning on survival, its residual values and histopathology in certain organs of a freshwater airbreathing fish, Channa punctatus (BLOCH ) were investigated. The threshold concentration, MATC and LC₅₀ values obtained from 96 h static bioassay, revealed that Channa is more susceptible to cadmium ions at higher temperature. The atomic absorption spectrophotometric determination of cadmium residues differed significantly in organs of specimens having survived and died after 96 h of exposure. The gill accumulate the highest amount of cadmium, the liver accumulated a slightly smaller amount than the gill, while the kidney accumulated the least. The histopathological lesions subjected to sublethal (5.2 mg/l Cd) and lethal (8.4 mg/l Cd) concentrations of cadmium were: detachment and rupture of lamellar epithelium, collapse of pillar cells and hypertrophy in mucus producing gland cells in the gill; vacuolization and coagulative necrosis in hepatic cells of the liver; and expansion, necrosis and accumulation of cellular debris in renal tubules of the kidney. The probable causes for death of fish due to cadmium ions have been discussed.     

Acute toxicity of platinum to coho salmon (Oncorhynchus kisutch)

The effects of short-term exposure to tetravalent platinum on survival, opercular movement and post-treatment growth of coho salmon fry (Oncorhynchus kisutch) was investigated. Employing a static water acute toxicity bioassay with platinum as PtCl₄2HCl·6 H₂O, at 8.5±0.2°C, and a water hardness of 55.9±3.5 mg l.^?1 (as CaCo₃), the 24, 48, and 96-h LC₅₀ values were 15.5, 5.2, and 2.5 mg Pt⁴⁺ l.^?1 respectively.Rates of opercular movement for fish exposed to platinum increased with increasing concentrations to a level of 1.0 mg l.^?1. No further significant increases were evident above this level. Hypoactivity of fish exposed to 0.3 mg l.^?1 and higher was evident during the acute toxicity bioassay and much of the post-treatment study. Post-treatment rate of growth for fish exposed to sublethal concentration of platinum for 96 h was less than that of the controls.All organs examined histopathologically were within normal ranges with the exception of the gills and olfactory organ. Lesions in fish exposed to concentration of 0.3 mg l.^?1 and higher were characterized by branchial epithelial hypertrophy and hyperplasia, and necrosis of olfactory epithelial cells.     

Comparison of the behavioural and acute toxicity of copper to sheepshead,Atlantic croaker and pinfish

Sheepshead (Archosargus probatocephalus), Atlantic croaker (Micropogon undulatus) and pinfish (Lagodon rhomboides) were each exposed to aqueous solutions of reagent grade cupric chloride in artificial seawater in four static bioassays. The 24, 48, 72 and 96-h TL_m were determined to be, respectively, 4.17, 3.02, 2.19 and 1.14 mg Cu²⁺·I^?1 for sheepshead, 9.15, 7.55, 7.20 and 5.66 mg Cu²⁺·I^?1 for croaker, and 7.33, 4.43, 4.14 and 2.75 mg Cu²⁺·I^?1 for pinfish. These results are compared with literature values of experimental concentrations of copper producing subtle behavioural changes in these species.     

Acute and long-term toxicity of cadmium to Idothea baltica (crustacea,isopoda)

Acute toxicity tests of cadmium on Idothea baltica demonstrated differences among females, males and juveniles. Chronic toxicity tests showed that a sublethal concentration of cadmium sulphate administered during embryonic and/or larval development causes reduction of growth and increased death rate. A paradoxical effect was observed in juveniles treated during embryonic development, which in the first 60 days of life showed a temporarily significant increase in body size, as compared with controls. The criteria for establishing water quality in order to guarantee protection of the environment are discussed.     

Acute toxicity of the insecticide cypermethrin to three common European mayfly and stonefly nymphs

Freshwaters are particularly vulnerable to pesticide contamination from adjacent agriculture and forestry. Cypermethrin is a commonly used pyrethroid insecticide, but information on its toxicity to non-target freshwater invertebrates is limited to a small number of taxa. We assessed cypermethrin toxicity to nymphs of the common European mayflies Baetis rhodani and Ecdyonurus venosus, and the stonefly Isoperla grammatica in 96 h static bioassays. The 96 h median lethal concentrations (LC50) we recorded for the three species were 0.08 μg/L, 0.15 μg/L, and 0.13 μg/L, respectively. The effective concentration (EC50), quantified as lack of escape response (implying inability to avoid drift, predation or abiotic stress), was usually 32 % of the LC50. All 96 h LC50 values were lower than the maximum concentrations detected in streams in UK and continental Europe. Alarmingly, the 96 h LC50 for B. rhodani was below the currently applied single-pesticide EU safe drinking water standard of 0.1 μg/L. Contrasts among species were most evident at 24 h LC50, when B. rhodani was approximately ten times more sensitive than the other two taxa. B. rhodani is a key algal grazer, ubiquitous in European streams, and its decline could exacerbate algal blooms. The order of taxa sensitivity to cypermethrin in our study contrasts with their sensitivity to organic pollution in the same region, which suggests a tolerance trade-off, and thus a compounding effect of simultaneous stressors. Mayflies and stoneflies are important prey for instream and riparian predators, and key components of most eutrophication indices globally, all of which could be compromised in presence of cypermethrin.     

Acute toxicity of the water-soluble fraction of Cook Inlet crude oil to the Manila clam

Recognition of the inherent complexities of oil bioassays prompted the development of several apparatuses designed to deliver uniform solutions of the water-soluble components of crude oil and capable of system equilibration for both static and continuous-flow bioassays without the loss of the more volatile compounds and without the formation of emulsions. Clams from a relatively contaminated area exhibited greater resistance to the water-soluble fraction of Cook Inlet crude oil than specimens from a more pristine area. Behaviour was altered below lethal levels suggesting the possibility of using behavioural modifications as a concommitant criterion of toxicity with mortality. An increase in mortality with a longer exposure period and a significant rate of latent mortalities following the end of the 96-h exposure period suggest that future bioassays with bivalves should be determined over a longer exposure period.     

Copper toxicity in the marine copepod Tigropus japonicus: low variability and high reproducibility of repeated acute and life-cycle tests

The intertidal copeopod Tigriopus japonicus, which is abundant and widely distributed along the coasts of Western Pacific, has been suggested to be a good marine ecotoxicity testing organism. In this study, a series of experiments were conducted to investigate the reproducibility and variability of copper (Cu) sensitivity of T. japonicus so as to evaluate its potential to serve as an appropriate test species. To understand the seasonal variation of Cu sensitivity, individuals of T. japonicus were collected from the field in summer and winter, and subjected to standard 96 h acute (static renewal) toxicity tests. 96 h-LC50 values of T. japonicus collected from the two seasons were marginally different (p = 0.05), with an overall coefficient of variation (CV) of 33%. Most importantly, our results indicated that chronic Cu sensitivity of T. japonicus was highly reproducible. The CVs of intrinsic rates of increase in the population of the control and Cu treatment (10 microg Cu l(-1)) groups were only 10-11% between 10 runs of a standardised complete life-cycle test. Moreover, different Cu(II) salts generally resulted in a similar 96 h-LC50 value while Cu(I) chloride was consistently slightly less toxic than Cu(II) salts. Given such a high reproducibility of toxic responses, it is advocated to use T. japonicus as a routine testing organism.     

Copper toxicity in the marine copepod Tigropus japonicus: Low variability and high reproducibility of repeated acute and life-cycle tests

The intertidal copeopod Tigriopus japonicus, which is abundant and widely distributed along the coasts of Western Pacific, has been suggested to be a good marine ecotoxicity testing organism. In this study, a series of experiments were conducted to investigate the reproducibility and variability of copper (Cu) sensitivity of T. japonicus so as to evaluate its potential to serve as an appropriate test species. To understand the seasonal variation of Cu sensitivity, individuals of T. japonicus were collected from the field in summer and winter, and subjected to standard 96 h acute (static renewal) toxicity tests. 96 h-LC50 values of T. japonicus collected from the two seasons were marginally different (p = 0.05), with an overall coefficient of variation (CV) of 33%. Most importantly, our results indicated that chronic Cu sensitivity of T. japonicus was highly reproducible. The CVs of intrinsic rates of increase in the population of the control and Cu treatment (10 μg Cu l⁻¹) groups were only 10–11% between 10 runs of a standardised complete life-cycle test. Moreover, different Cu(II) salts generally resulted in a similar 96 h-LC50 value while Cu(I) chloride was consistently slightly less toxic than Cu(II) salts. Given such a high reproducibility of toxic responses, it is advocated to use T. japonicus as a routine testing organism.     

Predicting copper toxicity in estuarine and marine waters using the Biotic Ligand Model

The Biotic Ligand Model (BLM) has proven efficient in predicting the toxicity of a variety of metals to freshwater organisms. Consequently, the US EPA has proposed its use for calculating freshwater copper criteria. This study evaluates the BLM for use in estuarine and marine waters. Studies were conducted using the bivalve, Mytilus sp. and 48-h embryo-larval development chronic estimator test methods. These are the most sensitive taxa and test in the US EPA saltwater copper criteria database. Samples from five locations around the USA were tested. There is a strong relationship between measured and BLM predicted copper EC50s (log transformed data, r² = 0.76, p < 0.001, n = 44). The BLM predicted within a factor ±2 of measured EC50s in 41 of 44 cases. However, the BLM tends to predict lower EC50s when measured EC50s are approximately 10 μg Cu/L. This may be due to limitations of the metal-dissolved organic matter interaction model.     

Copper toxicity in the marine copepod Tigropus japonicus: Low variability and high reproducibility of repeated acute and life-cycle tests

The intertidal copeopod Tigriopus japonicus, which is abundant and widely distributed along the coasts of Western Pacific, has been suggested to be a good marine ecotoxicity testing organism. In this study, a series of experiments were conducted to investigate the reproducibility and variability of copper (Cu) sensitivity of T. japonicus so as to evaluate its potential to serve as an appropriate test species. To understand the seasonal variation of Cu sensitivity, individuals of T. japonicus were collected from the field in summer and winter, and subjected to standard 96 h acute (static renewal) toxicity tests. 96 h-LC50 values of T. japonicus collected from the two seasons were marginally different (p = 0.05), with an overall coefficient of variation (CV) of 33%. Most importantly, our results indicated that chronic Cu sensitivity of T. japonicus was highly reproducible. The CVs of intrinsic rates of increase in the population of the control and Cu treatment (10 μg Cu l⁻¹) groups were only 10–11% between 10 runs of a standardised complete life-cycle test. Moreover, different Cu(II) salts generally resulted in a similar 96 h-LC50 value while Cu(I) chloride was consistently slightly less toxic than Cu(II) salts. Given such a high reproducibility of toxic responses, it is advocated to use T. japonicus as a routine testing organism.