The effects of recombinant eel growth hormone,methyltestosterone and L-thyroxine on the growth of red sea bream,Pagrosomus major

the effects of recombinant eel growth hormone (reGH), methyltestosterone (MT) and L-thyroxine (T4) on the growth of red sea bream,Pagrosomus major, were investigated. Administration of reGH to fry by immersion at 2 mg/1 for 2 h every 5 days resulted in significant increase in both weight and length, but the condition factor (CF) diminished relative to that of similarly treated controls over the 37 day treatment period. Immersion in 0.1 mg/l T4 also resulted in significant increase in both weight and length and higher survival rate of test fry compared to the controls. Immersion in MT had less effect on growth and high-dose resulted in high mortality. In the second study, injection of 2 μg reGH/(g·wk) caused a significant increase in the specific growth rate (SGR) of test red sea bream fingerlings relative to that of the controls during the 4—week treatment period and maintained the increasing trend over the post-treatment period (weeks 4–6). Injection of MT at a dosage of 1μg/(g·wk) resulted in a significant increase in SGR during the 4—week treatment period. Intramuscular injection of 0.1 μg T4/(g·wk) also resulted in a significant increase in weight. Injection of high-doses of MT and T4 inhibited growth and resulted in darkening skin, bulging eyes and thinning body. In muscle chemical composition, the treated groups had no significant differences compared to the controls, but the high-dose MT and T4 groups showed significant increases in lipid content. Potential practical methods for hormone application in fish culture are discussed.     

Chromosome behavior of heat shock induced triploid in Fenneropenaeus chinensis

Triploidy was induced in Chinese shrimpFenneropenaeus chinensis by 30±0.5°C heat shock treatment (initiated at 20 min after fertilization) for 10 min to inhibit the release of PB2 at 18.0°C. The highest triploid rate obtained was 84.5% in nauplius stage. The effect of heat shock treatment on meiosis and cleavage of eggs was investigated in this work aimed to establish efficient procedures for triploid induction and to gain understanding of the mechanism of triploid production. Three pronuclei that could be observed in the treated eggs under fluorescence microscope developed into triploid embryos. Some abnormal chromosome behavior was observed in heat shocked eggs. Contribution No. 4407 from the Institute of Oceanology, Chinese Academy of Sciences. This work was supported by Project under Major State Basic Development Program (G1999012009) and Knowledge Innovation Program of the Chinese Academy of Sciences (KZCX 2-211)     

GROWTH PROMOTION OF RED SEA BREAM, PAGROSOMUS MAJOR, BY ORAL ADMINISTRATION OF RECOMBINANT EEL AND SALMON GROWTH HORMONE

Recombinant eel GH and yeast containing chinook salmon growth hormone (reGHand rcsGH) were incorporated into gelatin and sodium alginate (reGH-GS and rcsGH-GS) or polymer ma-trix (reGH-HP55) to protect the hormone from proteolytic cleavage in the stomach. The diets containin greGH-GS, rcsGH-GS, reGH-HP55 and free-reGH or uncoated-rcsGH were administered to red sea bream. Feeding of reGH-GS, reGH-HP55 and rcsGH-GS diets resulted in significant increases in body weight and fork length over those of controls. These results strongly suggest that gelatin and sodium algi-nate as well as polymer matrix protected the hormone from proteolytic enzymes in the gastrointestinal tract to allow the bioactive hormone to enter the circulation and eventually stimulato fish growth.     

CYTOTOXICITY AND GENOTOXICITY OF POLYETHYLENIMINE AND NICKEL CHLORIDE IN RED SEA BREAM（Pagrosomus major）FIN CELL LINE RSBF

A continuous marine fish cell line RSBF(i.e.Red Sea Bream Fin)was utilized to screen the cytotoxicity and genotoxicity of polyethylenimine(PEI)and nickel cholride(NiCl2)in this study on the deleterious effects of aquatic genotoxins on fish.At the 0.01 to 1μgml concentration tested,PEI had acute toxicity to the treated RSBF cells(IC50=1.12,0.92,0.88 and 0.64μg/ml PEI for time 0 h,24 h,48 h and 72 h after treatment,respectively)and markedly inhibited their proliferation in a dose-dependent manner.At the 0.001 to 5 μmol/L concentration tested,NiCl2 posed no acute toxicity but significantly stimulated their growth(107?214?of control).Random amplified polymorphic DNA(RAPD)technique was used to detect the genotoxic effects of PEI and NiCl2 by comparing the RAPD banding patterns of the control and treated cells.RAPD analysis indicated that at the concentrations tested,PEI was more genotoxic than NiCl2 to RSBF cells;that there was a slight dose-dependent response in the genotoxic effect of PEI bue not NiCl2;and that RAPD technique might provide a sensitive,non-specif-ic gentoxic endpoint.And the potent cytotoxicity and genotoxicity of PEI on fish cells showed that we should be cautious in utilizing it as gene vector in fish gene transfer and human gene therapy.     

Seasonal variation of the glycogen enzyme activity in diploid and triploid Pacific oyster gonad during sexual maturation

The glycogen content and the activities of two key enzymes in glycogen metabolism, glycogen phosphorylase and gly- cogen synthetase, in the gonad of diploid and triploid Pacific oysters (Crassostrea gigas) were compared during maturation. The glycogen content in the gonad of diploids decreased with gametogenesis (by 85.7%), but the glycogen content in the gonad of trip- loids did not vary significantly. Activity of glycogen phosphorylase (GP) in the gonad of diploids decreased with gametogenesis (by 55.5%), while GP activity of triploids did not vary significantly during maturation. Activity of glycogen synthetase (GS) in the gonad of diploids increased slightly with gametogenesis, reaching a peak in June. Activity of GS declined sharply from June to July, which might be due to gonad spawning. GS activity of triploid oysters in spawning time (July and August) was significantly higher than that in other months, which might be explained with a ‘compensating’ mechanism for the higher glycogen content in triploids.     

Chromosome Behavior of Heat Shock Induced Triploid in Fenneropenaeus Chinensis

INTRODUCTIONTriploidanimalsareusefulbecausetheyaresterile ,characterizedbypositivegrowthinthere productiveseason ,usefulfortesting ,forintroductionofnon nativespecies ,andforprotectionofde velopedstrains.Theinductionoftriploidyhadbeenreportedinmanyaquaculturespecies .Triploidshavebeensuccessfullyinducedinshrimpusingtemperatureshockorchemicalshock (CB ,6 DMAP)in 4species :Sicyoniaingentis (Xiangetal.,1 991 ) ,Fenneropenaeuschinensis (Xiangetal.,1 992 ;Daietal.,1 993;Baoetal.,1 993;Li…     

An experimental study on major element release from the sediments in the Changjiang (Yangtze River) Estuary

With the enhanced warming and acidification of global ocean, whether and to what extent the naturally-weathered fluvial sediment into the sea can release elements and thus influence the geochemical pro...     

STUDY ON EMBRYONIC DEVELOPMENT AND EARLY GROWTH OF TRIPLOID AND GYNOGENETIC DIPLOID LEFI—EYED FLOUNDER, PARALICHTHYS OLIVACEUS （T. et S. ）

The early effects of chromosomal manipulation of eggs and sperm on the yields of trip-loid and gynogenefic diploid larvae of Paralichthys olivaceus were investigated. Triploidy was achieved by cold shocking fen.ilized eggs at 0 - 2℃ for 45 minutes duration 5 minutes after fen.ilization, and the in-duced triploidy rates were 31.2% - 50% and the relative hatching rates were 53.3% - 99%. Gynnge-aetic diploids were obtained when eggs were inseminated with irradiated sperm and cold shocked at 0 -2℃ for 45 minutes duration 5 minutes after fertihzation. The induced gynogenetic diploid rates and the relative hatching rates were 94 % - 96 % and 48.5 % - 68.5 % respectively. The embryonic development of the triploid experimental group and of the gynogenetic diploid experimental group was delayed at first compared with the control group. But from the gastrula stage, it was not delayed anymore. There were no significant differences in the growth of the triploid experimental group larvae and the control group larvae, and in the growth of the gynogenetic diploid experimental group larvae and the control group larvae ac-cording to Student‘s t-test (α = 0.05). The relationship between the early growth of the triploid experi-mental group larvae and that of gynogenetic diploid experimental group larvae was also studied.     

Studies on the phosphorus requirement and proper calcium/phosphorus ratio in the diet of the black sea bream (Sparus macrocephalus)

An expriment on the phosphorus requirement and the proper Ca/P ratio in the diet of the black sea bream using the phosphorus gradient method (with casein as basic diet, sodium dihydrogen phosphate as source of phosphorus, and calcium lactate as source of calcium) showed that growth was greatly affected by the diet's phosphorus content and Ca/P ratio. Inadequate phosphorus in the diet resulted in slow growth and poor food conversion ratio (FCR). Analyses of the fish body showed it contained a high level of lipid but a low level of moisture, ash, calcium and phosphorus. The optimal values of phosphorus and Ca/P ratio in the black sea bream diet are 0.68% and 1∶2 respectively. Phosphorus in excess of this optimum value resulted in slow growth or even death. The results of this experiment clearly indicated that phosphorus is the principal mineral additive affecting black sea bream growth. Contribution No. 1972 from the Institute of Oceanology, Academia Sinica.     

Preliminary results on relationship between thermal diffusivity and porosity of sea ice in the Antarctic

The in situ sea-ice temperature, salinity and density observed from Chinese Antarctic Zhongshan Station have been applied to calculate the vertical profile of sea ice porosity. Based on numerical method, a number of schemes on sea-ice thermal diffusivity versus porosity have been accessed and one optimized scheme is identified by an optimal control model with an advanced distributing parameter system. For simplicity, the internal heating source item was neglected in the heat conduction equation during the identification procedure. In order to illustrate the applicability of this identified scheme, the vertical ice temperature profiles have been simulated and compared with measurements, respectively by using identified scheme and by classical thermodynamic formulae.The comparisons indicated that the scheme describing sea-ice thermal diffusivity and porosity is reasonable. In spite of a minor improvement of accuracy of results against in situ data, the identified scheme has a more physical meaning and could be used potentially in various applications.     

Growth, metabolism and physiological response of the sea cucumber, Apostichopus japonicus Selenka during periods of inactivity

The growth, metabolism and physiological response of the sea cucumber, Apostichopus japonicus, were investigated during periods of inactivity. The body weight, oxygen consumption rate (OCR), activities of acidic phosphatase (ACP), alkaline phosphatase (AKP), catalase (CAT) and superoxide dismutase (SOD), and content of heat shock protein 70 (Hsp70) in the body wall and coelomic fluid of A. japonicus were measured during starvation, experimental aestivation and aestivation. The results showed that the body weight of sea cucumber in the three treatments decreased significantly during the experimental period (P < 0.05). The OCR of sea cucumber reduced in starvation and experimental aestivation treatments, but increased gradually in natural aestivation treatment. The activities of ACP and AKP of sea cucumber decreased gradually in all treatments, whereas those of SOD and CAT as well as Hsp70 content decreased in the starvation and experimental aestivation treatments and increased in natural aestivation treatment. The sea cucumber entered a state of aestivation at 24℃. To some extent, the animals in experimental aestivation were different from those in natural aestivation in metabolism and physiological response. These findings suggested that the aestivation mechanism of A. japonicus is complex and may not be attributed to the elevated temperature only.     

Effects of water depth and substrate color on the growth and body color of the red sea cucumber,Apostichopus japonicus

Three color variants of the sea cucumber,Apostichopus japonicus are recognized,the red one is highly valued in the market. When the red variant is cultured in ponds in China,its body color changes from red to celadon in 3–6 months. The effects of water depth and substrate color on the growth and body color of this animal were investigated. Juveniles of red A. japonicus were cultured in cages suspended at a range of water depths(20,50,100,150 and 200 cm). The specific growth rate of red sea cucumbers was significantly higher in animals cultured at deeper water layers compared with those grown at shallowers. Body weights were greatest for sea cucumbers cultured at a depth of 150 cm and their survival rates were highest at a depth of 200 cm. A scale to evaluate the color of red sea cucumbers(R value) was developed using a Pantone standard color card. All stocked animals in the 9-month trial retained a red color,however the red body color was much more intense in sea cucumbers cultured at shallower depths,while animals suspended in deeper layers became pale. In a separate trial,A. japonicus were cultured in suspended cages with seven different colored substrates. Substrate color had a significant effect on the growth and body-color of red A. japonicus. The yield were greatest for A. japonicus cultured on a yellow substrate,followed by green white orange red black and blue. All sea cucumbers in the 7-month trial retained a red color,although the red was most intense(highest R value) in animals cultured on a blue substrate and pale(lowest R value) for animals cultured on a green substrate.     

Studies on the optimum protein content and the ratio of animal to plant protein in the diet of the black sea bream (Sparus macrocephalus)

An experiment on the optimum dietary protein content for the black sea bream using semi-purified diets showed the optimum is 50.19% by weight as inferred from the growth rate and food consumption of the fish. Another experiment on the proper ratio of animal to plant protein in its diet using sandeel as the animal protein source and peanut cake meal as plant protein source showed that the ratio had great effect on its growth and should be 1.00: 0.89–0.96 as judged from the growth rate and food consumption of the fish. Contribution No. 1973 from the Institute of Oceanology, Chinese Academy of Sciences.     

Preliminary study on the erratic exposure ages of Grove Mountains, East Antarctica

The first study of erratic boulder exposure ages in the Grove Mountains,interior Antarctica,indicates the two erratic boulders(060131-1 and 060131-2,collected from a typical nunataks,Zakharoff Ridge in the Grove Mountains) have 10Be minimum exposure ages of 1.24±0.11 Ma,1.37±0.12 Ma,and 26Al ages of 0.90±0.12 Ma,0.44±0.04 Ma,respectively.Meanwhile,another erratic boulder sample 060131-4,coming from vicinal ice surface,has 10Be and 26Al minimum exposure ages of 0.47±0.3 Ma and 0.44±0.04 Ma,respectively.The e...     

Effect of n-3 HUFA levels in rotifers and Artemia on growth and survival of larval black sea bream （Sparus macrocephalus）

Requirement for dietary n-3 HUFA (n-3 highly unsaturated fatty acid) for growth and survival of black sea bream (Sparus macrocephalus) larvae was studied using rotifers and Artemia at various levels of n-3 HUFA. Five treatments with rotifers and Artemia differing in n-3 HUFA were prepared by enriching them with various oil emulsions. Results indicated that dietary n-3 HUFA significantly influence fish n-3 HUFA levels and are essential for growth and survival of black sea bream larvae. The results also indicated that the incorporation of n-3HUFA TG (triacylglycerols) into tissues of larval black sea bream was more effective from natural fish oil in comparison with n-3 HUFA fatty acid ethyl esters from ethyl-esterified oil.     

NUMERICAL STUDY ON THE INTERANNUAL OSCILLATION OF SEA SURFACE TEMPERATURE IN THE SOUTH CHINA SEA

A two and a half layer oceanic model of wind-driven, thermodynamical general circulation is appliedto study the interannual oscillation of sea surface temperature (SST) in the South China Sea (SCS). Themodel consists of two active layers: the upper mixed layer (UML) and the seasonal thermocline, with themotionless abyss beneath them. The governing equations which include momentum, continuity and sea.temperature for each active layer, can describe the physics of Boussinseq approximation, reduced gravityand equatorial β-plane. The formulas for the heat flux at the surface and at the interface between twoactive layers are designed on the Haney scheme. The entrainment and detrainment at the bottom of theUML induces vertical transport of mass,momentum and heat, and couples of dynamic andthermodynamic effect.Using leap-frog integrating scheme and the Arakawa-C grid the model is forced bya time-dependent wind anomaly stress pattern obtained from category analysis of COADS. The numerical results indicate that t     

中国东部末次冰期雪线高程重建与成冰气候分析

在蒙山第四纪冰川遗迹与光释光测年研究的基础上,结合以往我国东部猛犸象化石出土位置、孢粉数据库、以及雪线高程的研究成果,首次对我国东部末次冰期时的雪线高程进行了恢复重建,并绘制了我国东部及东亚地区雪线高程图,在此基础上对我国东部蒙山冰期时的气候特征进行了探讨,认为我国东部冰期时的北路寒潮形成的"东亚冷槽",是我国东部第四纪山谷冰川的表现形式及形成原因。     

Preliminary study on plasma membrane fluidity of Psychrophilic Yeast Rhodotorula sp.NJ298 in low temperature

The ability of cell to modulate the fluidity of plasma membrane was crucial to the survival of microorganism at low temperature. Plasma membrane proteins, fatty acids and carotenoids profiles of Antarctic psychrophilc yeast Rhodotorula sp. NJ298 were investigated at -3 ℃, 0 ℃ and 8 ℃. The results showed that plasma membrane protein content was greater at -3 ℃ than that at 8 ℃, and a unique membrane polypeptide composition with an apparent molecular mass of 94.7 kDa was newly synthesized with SDS-PAGE analysis; GC analysis showed that the main changes of fatty acids were the percentage of unsaturated fatty acids (C18∶ 1 and C18∶ 2) and shorter chain saturated fatty acid (C10∶ 0) increased along with the decrease of the culture temperature from 8 ℃ to -3 ℃; HPLC analysis indicated that astaxanthin was the major functional carotenoids of the plasma membrane, percentage of which increased from 54.6±1.5% at 8 ℃ to 81.9±2.1% at -3 ℃. However the fluidity of plasma membrane which was determined by measuring fluorescence anisotropy was similar at -3 ℃, 0 ℃ and 8 ℃. Hence these changes in plasma membrane's characteristics were involved in the cellular cold-adaptation by which NJ298 could maintain normal plasma membrane fluidity at near-freezing temperature.     

Preliminary study on particulate organic carbon export fluxes in the Bering Sea

During the Second Chinese National Arctic Expedition (CHINARE) from July to September 2003, depth profiles of dissolved and particulate 234Th in upper water columns were collected at two stations of BR03 and BR24 in the Bering Sea. 234Th was sampled by using a traditional Fe(OH)3 co-precipitation technique, which is a reliable approach to 234Th measurement. We observed 234Th excess at station BR03 below the euphotic zone, which was possibly due to the intensive remineralization of particulate matter. Particulate organic carbon (POC) export fluxes were estimated from a one-dimensional irreversible steady state model of 234Th fluxes together with measurements of the POC/234Th ratio on the suspended particles. The POC export fluxes from the euphotic zone were 11.66 and 11.69 mmol C m-2 d-1 at BR03 and BR24 stations,respectively. The ratios of POC fluxes to primary production at the two stations were about 0.5 and 0.59, respectively, probably due to the presence of large phytoplankton (in particular diatoms).     

A Preliminary study on traditional level-trench method to prevent rill initiation in the Three Gorges region, China

The level-trench method is a traditional tillage operation to develop a drainage system on slope land in the Three Gorges region.Before crops are planted,farmers prepare the land with level trenches spaced 3-10 m apart,depending on the slope gradient:steeper slopes require shorter distances.Little scientific analysis has been done on the use of traditional level trenches for soil conservation.We conducted a field investigation and simulation experiments,and present a comparison between the spacing of level trenches and the slope length required for rill initiation.The results indicate that the spacing of level trenches in farmland is close to the slope length required for rill initiation in the experimental plots,and regression models of trench spacing with the gradient and the slope length required for rill initiation with the gradient have similar formats;the coefficients of determination (R2) for the two equations are 0.99 and 0.88,respectively.There were some differences between the field survey and experiments,but we conclude that the traditional level-trench method has an important role in preventing rill initiation.