Chemical element composition and amphipod concentration function in Baikal littoral zone

Mass-spectrometry with inductively coupled plasma was used to determine the element composition of 19 amphipod species, most of which are widespread in the stony littoral of Lake Baikal. Amphipod composition was found to be dominated by Ca > P ≥ S > K ≥ Na > Cl > Mg > Sr ≥ Br ≥ Si. The concentrations of all elements determined in amphipods is greater than the respective concentrations in water. The amphipods were found to concentrate P > Br > Cu > Zn > Cd to the greatest extent relative to the element composition of water and Br > P ≥ I > Ca > S > Cl ≥ As > Sr relative to that of the stone substrate. The concentrations of Cr, Mn, Fe, Co, Cu, Zn, As, Mo, Cd, Pb, and Hg in 2003–2006 in the amphipods of the stony littoral of Baikal was not greater than their concentrations in the amphipods from conventionally non-polluted or weakly polluted aquatic ecosystems. The obtained results can be used as background values in environmental monitoring.     

The Acute Toxicity of Plant Origin Pesticides into the Freshwater Fish Channa punctatus

Mortality to fish Channa punctatus caused by the latices of four species of terrestrial plants belonging to family Euphorbiaceae and Apocynaceae have been reported. It was found that extremely dilute aqueous solutions of the latex were effective in killing the fishes. The ranking of toxicity of four plants was Nerium indicum > Euphorbia royleana > Thevetia peruviana > Jatropha gossypifolia. It has been suggested that these plant products cannot be used directly in freshwater ecosystem unless their detailed toxicity has been studied on the beneficial freshwater animals.     

Extreme variability of water table dynamics in temperate calcareous fens: Implications for biodiversity

Calcareous fens are minerotrophic peatlands with very high species diversity, and maintenance of the water table is assumed to be a key contributor to this diversity. However, this assumption is based on limited study of fen water table dynamics. Here we monitor water table fluctuation in distributed locations across three calcareous fens differing in hydrogeomorphic setting for three growing seasons. Water table position was extremely variable with absolute ranges of 89, > 100, and > 118 cm in the Riparian, Trough and Basin Fens, respectively, and was controlled by landscape position and weather variability. Areas adjacent to a second‐order stream experienced the least water table fluctuation, while the Basin Fen, at > 75 m from a surface water connection, was very prone to year‐to‐year precipitation differences. Mean and median water table values were found to be poor indicators of biologically relevant hydroperiods. We introduce the term ‘duration of initial growing season saturation’ as a potentially more useful statistic to relate to plant species distribution. Across the studied fens, this duration ranged from 1 to 14 weeks from the start of the growing season. The water table resided below the ground surface for between 0 and 22 weeks of the growing season across the calcareous fens and study period. These findings impart great differences in the development of oxidized rooting depths. Our results demonstrate that there is much more variation in calcareous fen hydrology than previously reported, and this variability has important implications for fen vegetation patterning and management. Copyright © 2011 John Wiley & Sons, Ltd.     

Additive toxicity of herbicide mixtures and comparative sensitivity of tropical benthic microalgae

Natural waters often contain complex mixtures of unknown contaminants potentially posing a threat to marine communities through chemical interactions. Here, acute effects of the photosystem II-inhibiting herbicides diuron, tebuthiuron, atrazine, simazine, and hexazinone, herbicide breakdown products (desethyl-atrazine (DEA) and 3,4-dichloroaniline (3,4-DCA)) and binary mixtures, were investigated using three tropical benthic microalgae; Navicula sp. and Cylindrotheca closterium (Ochrophyta) and Nephroselmis pyriformis (Chlorophyta), and one standard test species, Phaeodactylum tricornutum (Ochrophyta), in a high-throughput Maxi-Imaging-PAM bioassay (Maxi-IPAM). The order of toxicity was; diuron > hexazinone > tebuthiuron > atrazine > simazine > DEA > 3,4-DCA for all species. The tropical green alga N. pyriformis was up to 10-fold more sensitive than the diatoms tested here and reported for coral symbionts, and is recommended as a standard tropical test species for future research. All binary mixtures exhibited additive toxicity, and the use of herbicide equivalents (HEq) is therefore recommended in order to incorporate total-maximum-load measures for environmental regulatory purposes.     

Molluscicidal Activity of Euphorbiales against the Snail Indoplanorbis exustus

Mortality caused to the snail Indoplanorbis exustus by the latices of four species of plants belonging to the family euphorbiaceae has been reported. This snail is the vector of the liver fluke Fasciola hepatica and Fasciola gigantica. It was found that very low concentrations of the latex were effective in killing the snails. The order of toxicity of the four plants was E. royleana > E. antisyphilitica > E. lactea cristata > J. gossypiifolia. The toxic activity of these plants was marginally lost after extraction by organic solvents. The toxic effect of all the plants was both dose and time dependent. It has been suggested that the latex of these plants may eventually find use as molluscicides in the field.     

Plastic ingestion by fulmars and shearwaters at Sable Island,Nova Scotia,Canada

Plastic pollution is widespread in the marine environment, and plastic ingestion by seabirds is now widely reported for dozens of species. Beached Northern Fulmars, Great Shearwaters, Sooty Shearwaters and Cory’s Shearwaters are found on Sable Island, Nova Scotia, Canada regularly, and they can be used to assess plastic pollution. All species except Cory’s Shearwaters contained plastic debris in their gastrointestinal tracts. Northern Fulmars, Sooty Shearwaters and Great Shearwaters all showed high prevalence of plastic ingestion (>72%), with Northern Fulmars having the highest number and mass of plastics among the species examined. There was no difference in plastic ingestion between sexes or age classes. In all species user plastics made up the majority of the pieces found, with industrial pellets representing only a small proportion in the samples. Sable Island could be an important monitoring site for plastic pollution in Atlantic Canada.     

Mercury bioaccumulation in the spotted dogfish (Scyliorhinus canicula) from the Atlantic Ocean

The annual and life-cycle mercury bioaccumulation pattern in selected tissues of the economically relevant Elasmobranchii species Scyliorhinus canicula was studied, and the risks associated with its consumption evaluated.Preferential mercury bioaccumulation occurred in muscle tissue, and followed the order muscle > heart > liver > gills > pancreas. Total mercury in muscle tissue ranged from 0.13 mg kg⁻¹ (wwt) in 1+ year old males to 0.8 mg kg⁻¹ (wwt) in 8+ year old mature females, with no significant differences found between genders, and no clear lifespan bioaccumulation pattern observed, except for mature females. Organic mercury in the muscle ranged from 0.05 mg kg⁻¹ (wwt) to 0.52 mg kg⁻¹ (wwt), corresponding to an average of 70% of total mercury content. In mature females, a significant correlation (R = 0.99, P = 0.01) was found between size and organic mercury fraction, suggesting reproduction as an important factor controlling organic mercury bioaccumulation in the spotted dogfish.     

Bioindication of the environmental state of marine areas with the use of fish biomarkers

The study is focused on the effect of the concentrations of toxic elements Zn, Cu, As, Pb, Hg, and Cd in the muscles of six species of Black-Sea fish on the concentrations of modified forms of proteins and medium-molecular peptides in fish serum. Correlation analysis established that the effect of toxic elements on the concentration of modified forms of proteins decreases in the series Zn + Pb > As > Hg > Cu > Cd and that for medium-molecular peptides decrease in the series Pb > Cu > Zn > Hg > As + Cd. The possible use of the concentration of modified forms of proteins and medium-molecular peptides in fish blood as biomarkers for assessing their state and the effect of habitat on them.     

Antibiotic resistance of bacteria isolated from shrimp hatcheries and cultural ponds on Donghai Island, China

The resistance of bacteria to 12 different antibiotics was investigated in shrimp farms on Donghai Island, China. Antibiotic-resistant bacteria were found to be widespread in shrimp farms, indicating a high environmental risk. Further, significant differences were found in bacterial strains among farms (ANOVA, p < 0.05), showing resistance to antibiotics such as ampicillin, trimethoprim, compound sinomi, tetracycline, chloramphenicol and cefazolin. No significant differences in antibiotic resistance were found among 6 hatcheries evaluated in this study (ANOVA, p > 0.05), between exalted and traditional shrimp ponds (ANOVA, p > 0.05), and between cultural ponds and corresponding control water source sites (T-test, p > 0.05). In cultural ponds, no significant difference in bacterial resistance to antibiotics was found between water and sediment (T-test, p > 0.05), and antibiotic resistance of bacteria from water showed a significant positive correlation with that from sediment (p < 0.05). Therefore, our study indicates that bacterial multiple antibiotic resistance (MAR) is more widespread in shrimp hatcheries than ponds.     

Determination of Partition Coefficients of Metals in Natural Tropical Water

The values for the partition coefficient (K_d) were calculated for Ca, Mg, Cd, Cr, Cu, Fe, Mn, Pb, Ni, and Zn at 19 sites in the Capivara hydroelectric reservoir in Brazil. It was found that the relative values of K_d follow the order: Cr > Mn > Fe > Cu > Zn > Ni > Pb > Ca > Cd, differing from the values reported for K_d in aquatic systems in the northern hemisphere. A hierarchical cluster analysis and linear correlations showed that Cr is strongly associated with Fe and Cu, and that Cd is the only metal found in complexation with organic matter, explaining its higher solubility.     

Raindrop impact and water flow on the vegetative surfaces of trees and the effects on stemflow and throughfall generation

The physical processes of raindrop impact and water flow on the leaves and branches of selected tropical tree species were examined under laboratory conditions using simulated rainfall. Inclined branches were found to be particularly efficient in detaining impacting water droplets. This efficiency in raindrop detention increases linearly as a function of branch inclination on branches that are initially dry. On branches that have been thoroughly wetted, this increase in raindrop detention with branch inclination is best expressed as a logarithmic function. At branch inclinations of 60° above the horizontal, the quantity of branchflow was found to be > 80 per cent of the total quantity of impacting rainfall, indicating that < 20 per cent was lost to rainsplash. The strong positive relationship between branchflow and branch inclination explains why trees with large projected areas of steeply inclined upthrust branches have significant quantities of intercepted water draining from their branches and trunks under heavy rainfall conditions. This drainage from the woody frame of trees influences surficial processes by generating both stemflow and large throughfall droplets.     

Localization of Branchial Enzymes and their Significance in the Detection of Crude Oil Toxicity in Colisa fasciatus

The effect of crude oil on the histochemical localization of branchial enzymes CA, SDH, LDH, Alkpase and Acidpase was investigated in Colisa fasciatus. The activity of all the enzymes experimented here was found to have decreased when treated with the lethal solutions of crude oil except the LDH which showed an increasing trend throughout the experiment. A transitory increase in the CA and SDH was observed in the beginning but reached an almost normal level after 48 h in 1000 ppm. On the basis of sensitiveness towards crude oil toxicity, the enzymes have been arranged in the following order: LDH > SDH > CA > Alkpase > Acidpase. The results suggest that crude oil is capable of impairing the enzyme activity. The decreased or increased level of enzymes may be used as an indicator for assessing the toxicity of oil pollutants.     

Cumulative effects of land use on fish metrics in different types of running waters in Austria

The catchment land-use composition of 249 fish sampling sites in Austrian running waters revealed effects on the biological integrity. Beyond correlative analysis, we investigated (1) which land-use category had the strongest effect on fish, (2) whether metrics of functional fish guilds reacted differently, (3) whether there were cumulative effects of land-use categories, and (4) whether effects varied in strength across river types. We fed 5 land-use categories into regression trees to predict the European Fish Index or fish metric of intolerant species (mainly Salmo trutta fario). Agriculture and urbanisation were the best predictors and indicated significant effects at levels of >23.3 and >2%, respectively. Model performance was R ² = 0.15 with the Fish Index and R ² = 0.46 with intolerant species. The tree structure showed a cumulative effect from agriculture and urbanisation. For the intolerant species metric, a combination of high percentages for agriculture and urbanisation was related to moderate status, whereas <7.3% agriculture were related to good status, although urbanisation was higher than 1.8%. Headwater river types showed stronger responses to land use than river types of lower gradient and turned out to be more sensitive to urbanisation than agriculture.     

Water use strategies of the desert riparian forest plant community in the lower reaches of Heihe River Basin,China

The object of this paper is the different plant communities in the Ejina desert riparian forest.Groundwater depth,soil moisture,plant water potential,relative leaf moisture content and water use efficiency was monitored,and the response of soil moisture and plant ecology to the groundwater depth and the water use efficiency of the different plant communities was analyzed.The results showed that:(1)Along with the groundwater depth increasing,predawn and midday water potential of the plants,with the exception of Reaumuria soongorica,did not decrease significantly,indicating that when the groundwater depth is less than3 m,the plant communities in the range of 4 km from the river way did not suffer or slightly suffer from water stress;(2)The distribution of higher soil moisture content within 0–3 m soil layer is suitable with the plants’root system,as indicated in the communities of coexisting overripe Populus euphratica or Taramrix chinensis,both of which can release excessive water into soil for shallow rooted shrubs or herbaceous plants when there is water shortage;(3)R.soongorica can absorb deep soil moisture through deep roots for their own survival;(4)The community consisting of Sophora alopecuroides,Karelinia caspica,T.chinensis,and overripe P.euphratica has the best species combination for restoring the damaged eco-environment in the lower reaches of Heihe River;(5)The order of plants’relative leaf water contents is K.caspicaS.alopecuroidesyoung P.euphraticaoverripe P.euphraticamature P.euphratica=T.chinensis coexisting with other speciessingle R.soongoricasingle T.chinensis and the order of WUE is single T.chinensissingle R.soongoricaT.chinensis living in symbiosis with other speciesS.alopecuroides=young P.euphraticamature P.euphraticaoverripe P.euphraticaK.caspica.Therefore,with ample soil moisture,the plant community helps rapid growth of T.chinensis,young P.euphratica and R.soongorica plants of less moisture content.Despite this they do not have much water storage capability,but have strong drought resistance,and higher moisture contents of S.alopecuroides and K.caspica,thus leaving them with poor drought resistance.Overall,the desert riparian forest plant community in the lower reaches of Heihe River helps the species of higher WUE live on it.     

Polycyclic aromatic hydrocarbons in commercial fish and lobsters from the coastal waters of Madagascar following an oil spill in August 2009

Concentrations of polycyclic aromatic hydrocarbons were determined in species of commercial fish and lobsters following an oil-spill just off the protected Madagascan coastline. Samples were collected along the coastline within and outside the affected area. Summed PAH concentrations ranged from 1.9 μg kg⁻¹ to 63 μg kg⁻¹ wet weight, but with no higher molecular weight PAHs (>202 Da) being detected. All concentrations of benzo[a]pyrene, benz[a]anthracene and dibenz[a,h]anthracene were <0.1 μg kg⁻¹ wet weight, well within the EU and UK set limits for the protection of human health. Additionally, samples were calculated as the benzo[a]pyrene toxic equivalency quotient (TEQ) and found to be well below the level of concern in relation to health of human consumers. Evaluation of the biota PAH data indicated the origin of PAH was predominantly petrogenic with >80% arising from oil sources. Profile studies indicate a low-level multisource petrogenic contamination probably representing a pre-spill background for the area.     

Temporal and spatial biomonitoring of heavy metals in eastern Aegean coastal waters using Amphibalanus amphitrite

This biomonitoring study presents the spatial and temporal distributions of heavy metals in the soft tissues of a major fouling species Amphibalanus amphitrite living on hard substrate at different sites along the eastern Aegean coast. A. amphitrite has been chosen as a strong candidate for monitoring heavy metals. Sediment and seawater samples were also collected to detect their metal contents in order to gain more information on the environmental conditions and possible bioaccumulation patterns. The physico-chemical characteristics of sampling stations have been measured in order to characterize the sampling area. The order of metal concentrations in barnacles, sediment and seawater decreased in the following order Cu > Fe > Zn > Mn > Cd > Cr > Pb > Hg, Fe > Mn > Zn > Cu > Pb > Cr > Hg > Cd and Fe > Zn > Mn > Cu > Pb > Cr > Cd > Hg, respectively. These results showed that barnacles accumulate Cu in a higher degree than both sediment and seawater. Moreover, metal concentrations in barnacle have the potential for use in any future regulatory framework monitoring and eventually controlling ambient metal pollution levels.     

Population declines and heavy metal exposure of Swinhoe’s Storm Petrels (Oceanodroma monorhis) breeding on the southwest coast of Korea

We investigated the population dynamics of globally near-threatened Swinhoe’s Storm Petrels on Kugul and Chilbal islets where >90% of the world breeding population have resided for >25 years. We also determined the levels of six heavy metals in the seabird species as a possible factor affecting reproduction. Of the 892 and 307 marked burrows since 1986, the breeding density of Swinhoe’s Storm Petrels has changed significantly, based on a 55% decline at Kugul islet, and a 39% decline at Chilbal islet, suggesting that this species has suffered an extensive population decline. Of the individuals studied, five Swinhoe’s Storm Petrels and two Streaked Shearwaters had >8.0 μg/g Pb in tissues, which is a potentially lethal level in avian species. Given that substantial changes in their breeding populations may occur in a limited breeding colony, ecological threats including environmental pollutants warrant attention for global conservation of these birds.     

Autochthonous and allochthonous plant contributions to coastal benthic detritus deposits: a dual-stable isotope study in a volcanic lake

The high numbers of primary producers represent multiple sources of organic matter accumulating onto lake bottoms. The difficulty of distinguishing the relative contribution to the mixture presents considerable challenges to the analysis of these organic deposits. In this study, dual-stable isotope analysis and IsoSource model were used to identify allochthonous and autochthonous components of detritus deposits (Particulate Organic Matter: POM) at two different bottom slope sites of a volcanic lake (lake Bracciano). Experiments were carried out to calibrate IsoSource on constructed plant mixtures and assess changes in isotope ratios during plant decomposition. IsoSource satisfactorily discriminated the constructed mixture sources with a few exceptions. Changes in isotopic enrichment during decomposition were low, and thus did not represent a confounding variable in the isotopic analysis. By contrast, chemical and geological differences of the study sites were associated with differences in plant δ¹³C and δ¹⁵N values (more than 2‰ within single plant species). At both sites, the isotopic signals of POM fell between polygons delineated by source end members with an evident shift of δ¹³C toward allochthonous sources. POM amount and diversity were greater at the flatter bottom site, where allochthonous contributions were larger than at the other site. In particular, IsoSource ranked species contributions as follows: A. glutinosa > P. australis > A. donax > S. alba > P. nigra > the benthic macroalga Chara sp. at the first site, and A. glutinosa > P. nigra > the aquatic macrophyte C. demersum at the latter. The composition of littoral POM was determined by allochthonous sources in proportion to their relative abundances (as percent land cover) with differences between sites due to bottom slope.