Combined Effects of Temperature and Salinity on the Zoeal Development of the Crab Liocarcinus puber (Decapoda: Portunidae)

Abstract. Larval development of the crab Liocarcinus puber was studied from hatching to the megalopa stage under 12 different temperature and salinity combinations, 10°C, 15°C, and 20°C (T): 20, 25, 30, and 35% (S), with a view to establishing optimum conditions for zoeal development. High larval mortalities were recorded at 10°C in all the experimental salinities, indicating the sensitivity of the larvae to low temperatures. Highest zoeal survival was obtained in the higher salinity and temperature combinations. First zoeae completed their development in all T-S combinations tested, while successive stages showed increased sensitivity and suffered high mortalities in lower salinities at 15° and 20°C and in all salinities at 10°C. The duration of zoeal development decreased with increasing temperature, whilst the lower salinities delayed development. Q₁₀ values did not differ much at higher salinities, with values ranging from 1.5 to 2.6.     

Preparation and characterization of agar,agarose,and agaropectin from the red alga Ahnfeltia plicata

Agar,agarose,and agaropectin were extracted from the red alga Ahnfeltia plicata,and their properties and structures were characterized.Agar was extracted by a comparatively low alkaline consumption of 1.2%.It exhibited a gel strength of 1 152.50±74.25 g/cm^2 and a sulfate content of 0.55%±0.08%.The yield of agar from A.plicata was 24.53%,which is higher than those of other agarophytes commonly used in China.Three kinds of the method were compared for the purification of agarose,and the physicochemical properties of agarose that was prepared under the optimal condition were identical to those of commercially available agarose.Furthermore,agaropectin was purified from A.plicata and characterized by GC,HPLC,UV-spectrum,and FI-IR to understand its composition and structure.It was the first time to comprehensively study the agar and its fractions from the red alga of A.plicata.This research provided an eco-friendly agar extraction method from A.plicata and revealed its potential application for the production of agar,agarose,and agaropectin.     

南黄海夏季温盐年际变化时空模态与气候响应

根据南黄海断面1977—2016年历年8月标准层温度、盐度与气候要素观测资料,采用时空分析等方法,分析了南黄海断面夏季温度、盐度年际时空变化与气候响应。断面温度主要有4种时空模态,夏季风生环流、冷水团强度、面积与断面冬季温度模态是主要温度模态年际变化的主要影响因素;夏季风生流场形态、春季PDO指数与断面冬季温度模态是次要温度模态年际变化的主要影响因素;温度模态时间分量均为准平衡态长期变化。断面盐度主要有4种时空模态,夏季苏北沿海低盐度水体、南黄海中部高盐度水体与夏季黄海风生流输送作用是盐度主要模态年际变化的主要影响因素;夏季南黄海降水量减少与风生流输送减弱是盐度次要模态年际变化的主要影响因素。盐度主要模态时间分量为准平衡态长期变化,次要模态时间分量存在显著线性低盐趋势变化。断面夏季温盐多年平均分布主要受到夏季多年平均风生环流影响。断面核心冷水团月平均温度为准平衡态长期变化;面积存在显著线性减小趋势,黄海风生流场季节与年际变化是南黄海核心冷水团年际变化主要影响因素,春季PDO指数对冷水团面积年际变化有显著非线性影响。断面冷水团、核心冷水团月平均盐度为显著线性低盐趋势周期年际变化。由于黄海温盐长期线性趋势变化,与30多年前状况相比,目前黄海温盐场季节循环时空变化形态可能已经发生显著改变。     

Influence of combined changes in salinity and copper modulation on growth and copper uptake in the tropical green macroalga Ulva reticulata

The influence of salinity on growth and Cu uptake in the green macroalga Ulva reticulata collected from the intertidal area in the Western Indian Ocean was studied under controlled laboratory conditions. Exposure concentrations ranged from 5 to 500 μg Cu l⁻¹ at five salinities (ranging 20–40). The accumulation of Cu increased with decreasing salinity, so that the uptake at 500 μg Cu l⁻¹ was approximately 2.7, 2.4 and 2.0 times higher at salinities of 20, 25, and 30 respectively, than uptake at salinity of 35, and with uptake being lowest at salinity of 40. Ulva maintained a positive growth rate over the whole salinity range (20–40), with highest rates at salinity of 35. When exposing to Cu at low salinities (20 and 25), the growth rate of Ulva was strongly inhibited suggesting an increase in toxicity of Cu with decreasing salinity. EC50 and NOEC increased with increase in salinity, implying a reduced Cu toxicity at high salinities. It was concluded that salinity needs to be considered when using macroalgae, such as U. reticulata, as a bioindicator of heavy metals in areas with heavy rainfall, underground fresh water intrusion or in estuaries, as they might accumulate more metals and be more negatively affected.     

Effects of salinity on embryonic and larval development of Chinese mitten crab Eriocheir sinensis(Decapoda: Brachyura) and salinity-induced physiological changes

To investigate the effects of salinity on early development of Chinese mitten crab ( Eriocheir sinensis ), and the salinity tolerance mechanism of embryos, different developmental stages of embryos (gastrula, eyespot and pre-hatching stage), and hatched stage I zoea and megalopa, were exposed to a range of salinities (1, 5, 10, 15 (control), 20, 25, 30, 35 and 40). Hatching, survival and molting were monitored. Effects of 24-hour hypersaline (35) and hyposaline (1) stress on egg diameter, water content, Na +/K +-ATPase (NKA) activity, and crustacean hyperglycemic hormone (CHH) gene mRNA expression in embryos and megalopa, are reported. Embryos are more tolerant of low (≤ 5) than high (≥25) salinities, with optimum ranges for gastrula and pre-hatching stage embryos being 5-20, and for eyespot embryo and stage I zoea, 10-20. Most megalopa can molt to the first juvenile instar by day 5 at salinities between 1 and 40, whereas molting of megalopa stages was delayed at 40. Hypersaline conditions resulted in a loss of moisture, reduction of egg volume, and a signifi cant increase in NKA activity and CHH mRNA expression at some developmental stages. Hyposaline conditions did not affect moisture content or egg volume, but resulted in decreased NKA activity and CHH mRNA expression in embryos. For megalopa stages, NKA activity was significantly upregulated following both hypo- and hypersaline stress. Our results suggest high salinity will inhibit development and hatching of E. sinensis embryos, and low salinity will affect the survival of their stage I zoea. Increased NKA activity in embryos and megalopa stages might indicate a hyporegulation response under hypersaline conditions. These findings provide useful information for spawning ground protection of indigenous E . sinensis and enrich the knowledge of embryonic tolerance mechanisms of hyperregulating crustaceans following osmotic stress.     

提取江蓠琼脂新工艺条件的研究

提取江蓠琼脂新工艺条件的研究李来好，陈培基，王道公，吴燕燕（中国水产科学研究院南海水产研究所水产加工研究室）摘要采用＂高温稀碱法＂提取江蓠琼脂，系统地研究了江蓠的碱处理、漂白和提胶的新工艺条件。其主要特点是：（１）在碱处理中，添加了蒽衍生物，使琼脂的...     

Geochemistry of rare earth elements in the dissolved,acid-soluble and residual phases in surface waters of the Changjiang Estuary

The concentrations of rare earth elements in the dissolved, acid-soluble and residual phases in surface waters of the Changjiang Estuary were determined using ICP-MS. The main purposes of the study are to understand the estuarine geochemistry of rare earth elements and to explore water-particle interactions in the Changjiang estuarine mixing zone. The results show that there are two distinct processes operating on dissolved rare earth elements in the estuary: large scale removal at low salinities due to salt-induced coagulation and remarkable release at mid to high salinities. These processes result in modification of the effective river water flux and the systematical fractionation of the dissolved rare earth elements toward the East China Sea. The increase in concentration of dissolved rare earth elements in the mid to high salinity waters of the Changjiang Estuary suggests a sediment source in the mixing zone of the estuary, which is located over a shallow, broad shelf where there is extensive physical contact between bottom sediment and estuarine waters. Acid-soluble rare earth elements, the concentrations of which also dropped sharply in the low salinity region, appear to be controlled by salt-induced coagulation process and intense deposition of suspended particulate matter in the low salinity region. In the mid to high salinities, all acid-soluble rare earth element concentrations increase slightly with increasing salinity, suggesting that resuspension of sediments occurred. In contrast, the residual rare earth element concentrations are relatively constant with salinity variation in the Changjiang estuarine surface waters.     

盐度变化对铝牺牲阳极保护效果的影响

为研究河流入海口处盐度周期变化时铝牺牲阳极对钢结构的保护效果,测试对比了盐度固定与盐度循环条件下Q235钢有无阳极保护时的腐蚀失重和电位变化、铝牺牲阳极的电流效率.结果表明盐度循环会造成Q235钢的严重腐蚀,盐度差越大腐蚀越严重,甚至可能超过天然海水的腐蚀程度;在盐度循环条件下铝牺牲阳极对Q235钢具有良好的保护效果,...     

Impacts of Habitat Complexity on Physiology: Purple Shore Crabs Tolerate Osmotic Stress for Shelter

Purple shore crabs, Hemigrapsus nudus (Crustacea: Decapoda), can survive indefinitely in salinities of 8 (25% SW), but also tolerate short-term exposure to salinities as low as 2. In the laboratory their salinity preference range, determined from choice chamber experiments, is 22–32 and they can discriminate between pairs of salinities separated by a difference of 2. These crabs show a strong positive thigmotaxis and a weak negative phototaxis and tend to choose environments with available shelter. The presence of shelter significantly alters the behaviour of this species. When shelter is available the salinity preference range is 10–32. Even in salinities below this preference range, the presence of shelter prolongs the time spent in the lower test salinities. This change in behaviour has implications on the crab's physiology: the haemolymph osmolality falls to lower levels when crabs remain in low salinity under shelters. In the field, H. nudus is found in creeks with salinities close to freshwater and they may remain in this salinity for up to 11 h, if there are rocks under which to shelter. An increase in habitat complexity increases the number of crabs that are found within the creek. These crabs in the low salinity environment have a lower haemolymph osmolality than crabs on the nearby open shore. In H. nudus the behavioural selection of a shelter appears to outweigh the physiological costs associated with osmotic regulation of the body fluids. Therefore, the distribution of H. nudus in estuaries may depend more on the availability of suitable habitats rather than the salinity tolerance of this species.     

Influence of Bacterial Exopolymers, Conspecific Adult Extract and Salinity on the Cyprid Metamorphosis of Balanus amphitrite Cirripedia: Thoracica

Abstract. The influence of bacterial exopolymers and conspecific adult extract of Balanus amphitrite on metamorphosis of cyprid larvae at different salinities has been evaluated through laboratory assay. The bacterial exopolymers epm extracted from Pseudomonas, Acinetobacter and Enterobacteriaceae strains, isolated from the shell surface of Balanus amphitrite , were examined for their influence on metamorphosis, both individually and in combination with conspecific adult extract at 15, 25, 35 and 45% salinities. The epm extracted from the pool of these three strains mixed culture was also tested similarly.
The influence of epm varied with the strain of bacteria and salinity. The surface condition and time interval significantly influenced the metamorphosis rate. The interaction between these two variables, however, did not change in any combination, irrespective of the bacterial strain used. The Pseudomonas epm promoted metamorphosis at 15%, but was inhibitory at 45%. The Acinetobacter epm induced metamorphosis to a level of 71 ± 15% at 15%. No such metamorphosis promotion was observed with the epm of this strain at higher salinities and the influence was less than the conspecific adult extract. The epm of Enterobacteriaceae and mixed culture epm did not promote metamorphosis. There was no appreciable change in the metamorphic rate in the control conditions at 25%, 35% and 45% salinities and the rate was much lower than that obtained with conspecific adult extract. At 15%, however, the influence of adult extract was closer to that of the control condition. This shows that even in the absence of any attractant, the settlement intensity is positively influenced at mid-salinity of the barnacles' natural distributional range.     

Importance of vertical mixing for additional sources of nitrate and iron to surface waters of the Columbia River plume: Implications for biology

The influence of the Columbia River plume on the distributions of nitrate and iron and their sources to coastal and shelf waters were examined. In contrast to other large estuaries, the Columbia River is a unique study area as it supplies very little nitrate (5 μM) and iron (14–30 nM) at salinities of 1–2 to coastal waters. Elevated nitrate and dissolved iron concentrations (as high as 20 μM and 20 nM) were observed, however, in the near field Columbia River plume at salinities of 20. Surface nitrate concentrations were higher than observed in the Columbia River itself and therefore must be added by entrainment of higher nitrate concentrations from subsurface coastal waters. Tidal flow was identified as an important factor in determining the chemical constituents of the Columbia River plume. During the rising flood tide, nitrate and iron were entrained into the plume waters resulting in concentrations of 15 μM and 6 nM, respectively. Conversely, during the ebb tide the concentrations of nitrate and total dissolved iron were reduced to 0.3–3 μM and 1–2 nM, respectively, with a concomitant increase in chlorophyll a concentrations. As these plume waters moved offshore the plume drifted directly westward, over a nitrate depleted water mass (< 0.2 μM). The plume water was also identified to move southwards and offshore during upwelling conditions and nitrate concentrations in this far field plume were also depleted. Iron concentrations in the near-field Columbia River plume are sufficient to meet the biological demand. However, due to the low nitrate in the Columbia River itself, nitrate in the plume is primarily dependent on mixing with nitrate rich, cold, high salinity subsurface waters. Without such an additional source the plume rapidly becomes nitrate limited.     

Environmental tolerance of Caprella mutica: implications for its distribution as a marine non-native species

Physiological tolerances limit the distribution of marine species, with geographical ranges being set by environmental factors, such as temperature and salinity, which affect the rates of vital processes and survival of marine ectotherms. The physiological tolerances of the non-native marine amphipod Caprella mutica were investigated in laboratory experiments. Adult C. mutica were collected from a fish farm on the west coast of Scotland and exposed to a range of temperatures and salinities for 48 h. C. mutica were tolerant of a broad range of temperature and salinity conditions, with 100% mortality at 30 degrees C (48 h LT50, 28.3+/-0.4 degrees C), and salinities lower than 16 (48 h LC50, 18.7+/-0.2). Although lethargic at low temperatures (2 degrees C), no mortality was observed, and the species is known to survive at temperatures as low as -1.8 degrees C. The upper LC(50) was greater than the highest salinity tested (40), thus it is unlikely that salinity will limit the distribution of C. mutica in open coastal waters. However, the species will be excluded from brackish water environments such as the heads of sea lochs or estuaries. The physiological tolerances of C. mutica are beyond the physical conditions experienced in its native or introduced range and are thus unlikely to be the primary factors limiting its present distribution and future spread.     

盐度对香螺行为、鳃和肾抗氧化酶活性与组织结构的影响

为明确不同盐度胁迫下香螺的生理生态学响应及其适应机制,本文采用行为学、组织学和生物化学手段研究了不同盐度条件下(16、20、24、28、32、36、40、44、48)香螺的行为、病理损伤以及抗氧化酶活性。结果显示,在高盐和低盐胁迫下,香螺出现应激反应,摄食率下降,死亡率升高。随盐度升高,香螺的总抗氧化能力先升高后降低,在盐度32时达到峰值;香螺鳃的过氧化氢酶(CAT)活力和超氧化物歧化酶(SOD)活力呈先降低后升高的趋势;肾的CAT和SOD活力呈先升高后降低的趋势。组织切片结果显示,在高盐和低盐胁迫下,香螺嗅检器和鳃丝的形态呈现显著变化,黏液细胞数量均增加。上述结果表明,盐度显著影响香螺的生理生态反应,且香螺可通过行为变化、调节抗氧化酶以及改变组织形态结构从而适应外界盐度的变化。     

盐度对大叶藻种子萌发和幼苗发育的影响

大叶藻海草场是重要生境但在我国严重退化,亟需研究有效的修复方法。本文以桑沟湾的大叶藻种子为研究对象,探讨了盐度急变(0~30)对种子萌发、萌发后继续发育和存活及幼苗幼叶的光合与呼吸速率的影响,为以种子繁殖修复大叶藻海草场提供参考。结果表明:盐度降低促进种子萌发,盐度15及以下效果显著,盐度0时萌发率最高;盐度0~15中萌发的种子均可发育成幼苗,发育和衰亡的比例和速率受萌发盐度背景和萌发后培养盐度的双重影响——较高萌发盐度有利于种子萌发后的发育和存活,种子萌发后于盐度5~30中均可建成幼苗并存活、但在盐度0或1中发育至不同程度后全部死亡。盐度5~30范围内,幼苗幼叶均有净产氧,光合和呼吸作用及净产氧率具随盐度升高而增强趋势。于盐度0或5中萌发、盐度5~30中进一步培育的变盐育苗策略,最高实现了24%或12%的种子于2个月内建成幼苗并继续发育,可作为种子繁殖法修复大叶藻海草场的技术参考。     

紫菜胶与紫菜琼胶

研究了坛紫菜所含多糖的年率、性质和组成。用水直接提取的多糖产率为５．４５～６．８９％，无凝固性，含ＳＯ４１２．７３～１４．０８％，含３，６－ＡＧ９．９８～１２．１２％。经碱处理后提取的多糖产率为４．９～９．５％，最高的达１９．１％，它能形成很好的凝胶，其凝胶强度达７５０～１１００ｇ／ｃｍ＾２，含ＳＯ４０．２０～０．７５％，含３，６－ＡＧ４０．２８～４３．０４％，它的红外光谱和＾１３Ｃ－ＮＭＲ波谱与     

Characteristics of water mass under the surface mixed layer in Tsushima Straits and the southwestern Japan Sea in autumn

Two different cold waters were found under the surface mixed layer in Tsushima Straits and the southwestern Japan Sea in autumn 2004. One is cold saline water with a low concentration of dissolved oxygen, and the other is cold less saline water with a high concentration of dissolved oxygen. The older saline water originates from the bottom of the East China Sea, strongly influenced by the Kuroshio water with high salinity. The bottom density in the eastern channel of the Tsushima Straits is coincident with that of the East China Sea in autumn, corresponding to the season when the cold saline water was frequently found in the Tsushima Straits. The newer less saline water originates from the front of Tsushima Warm Current between the Tsushima Warm Current water and the surface cold water in the Japan Sea. This water is formed by subduction above the isopycnal surface from the front of the Tsushima Warm Current.     

台湾东北海域冷水块的特征

根据海洋调查历史资料 ,对台湾东北海域冷水块的时空分布、结构、特点进行分析 ,主要结果如下 :(1 )首次利用卫星跟踪漂流浮标的轨迹 ,佐证冬季该冷水块的存在。 (2 )鉴于该冷水的重要性 ,建议对它给予称谓 ,暂定名为“彭花棉”冷水块。 (3 )该冷水块是黑潮次表层水涌升的结果 ,是黑潮对我国近海海洋环境影响的典型实例之一。 (4)该冷水块具有低温、高盐、高密、低氧、高磷、高硅和高氮等特性     

龙须菜琼胶的提取方法研究

采用多种方法提取了龙须菜(Gracilaria lemaneiformis)中的琼胶,并研究了碱处理、微波处理、超声波处理等方法对龙须菜的琼胶提取率、硫酸基质量分数和多糖质量分数的影响。结果表明:当碱质量分数由2.5%增加到10%时,琼胶产率由15%降低到9.8%,硫酸基质量分数由2.66%降低到2.06%,同时,多糖质量分数由98.3%增加到102.6%;微波和超声波处理在不同程度上增加了琼胶的提取率和多糖质量分数,同时降低了硫酸基质量分数。另外,还从宏观和微观两个角度考察了龙须菜碱处理前后藻体的变化。     

Effects of sea ice formation and diapycnal mixing on the Okhotsk Sea intermediate water clarified with oxygen isotopes

Processes relating to the formation of dense shelf water and intermediate water in the Okhotsk Sea were studied by examining oxygen isotope ratios (δ¹⁸O), salinity, and temperature. The salinity and δ¹⁸O of the cold dense shelf water on the northern continental shelf showed peculiar relationship. The relationship indicates that 3% of the mixed-layer water, having salinity of 32.6, froze and the remaining 97% became dense shelf water of salinities of more than 33.2 (σ_θ>26.7) during the sea ice formation. The salinity–δ¹⁸O relationship also shows that 20% of the Okhotsk Sea Intermediate Water at the σ_θ=26.8 level was derived from the dense shelf water. The remaining 80% came from the Western Subarctic Pacific water modified by diapycnal mixing of water affected by the surface cooling and freshening within the Okhotsk Sea. The mixing with dense shelf water contributes to only 26% of the temperature difference or 8% of the salinity difference between the original Pacific water and the Okhotsk Sea Intermediate Water at σ_θ=26.8. This result suggests that the cold and less saline properties of the Okhotsk Sea Intermediate Water are produced mainly by diapycnal mixing, rather than by mixing of the Pacific water with the dense shelf water.