Identification of diverse bacterial communities for potential bio-aids capable of troubleshooting for wastewater biological treatment processes

Three commercial bio-aid samples have shown good troubleshooting capabilities earlier during several biological wastewaters processing, but their microbial contents were still unclear. This study was aimed to assess eubacteria contents for the three bio-aids based on sequence comparisons of 16S ribosomal deoxyribonucleic acid (rDNA). Majority of target 16S rDNA sequences of bacteria within or cultured from bio-aids were obtained via pyrosequencing-based approach, and few sequences were from isolation- or cloning-sequencing approaches. Results showed that a total of 8114, 2327, and 5402 sequences were retrieved from pyrosequencing-based methods for the three bio-aids, respectively, which were affiliated with 100, 80, and 59 bacterial species based on sequence comparison using Ribosome Data Project (RDP) Classifier and Basic Local Alignment Search Tool (BLAST). Target sequences matched Bacillus sp., Enterococcus sp., and Clostridium sp. of Firmicutes phylum were the most abundant for the bio-aids, while some sequences identical to bacteria of phyla Actinobacteria, Bacteroidetes, Chloroflexi, Cyanobacteria, and Proteobacteria were also found. Additionally, most of sequences from the isolation- or cloning-sequencing-based methods were not retrieved by the pyrosequencing-based methods. Culture media appeared to potentially select several bacteria species within phyla Firmicutes and/or Proteobacteria, but sequences from the culture-based methods did fulfill some absences for the uncultured method. Based on principal coordinate analysis, bacteria community structures of the three bio-aids were different. This study provided insights into bacteria contents for the three bio-aids as fundamental information in attempts for future selection or formulation of suitable bio-aids for solving various troubles during wastewater biological processing.     

Evaluation of the use of legumes for biodegradation of petroleum hydrocarbons in soil

The aim of this research was to evaluate the potential of six legumes: Medicago sativa L., Glycine max, Arachis hypogea, Lablab purpureus, Pheseolus vulgaris and Cajanus cajan to restore within a short period of time soil contaminated with 3% crude oil. The legumes in five replications were grown in crude oil-contaminated and crude oil-uncontaminated soil in a completely randomized design. Plants were assessed for seedling emergence, plant height and leaf number. GC–MS was used to analyze the residual crude oil from the rhizosphere of the legumes. Plant growth parameters were reduced significantly (P < 0.05) for legumes in contaminated soil compared to their controls. In the 4th week after planting (WAP), shoot height increased across the species up to the 8th WAP. However, in the 12 WAP no significant increase in the shoot of all species was observed. Two WAP legumes planted in contaminated soil had significantly (P < 0.05) higher leaf number than these planted in uncontaminated soil with the exception of M. sativa. In the 4th WAP, only A. hypogea and P. vulgaris had increased leaf number, while in the 6th WAP, only L. purpureus had increased leaf number and survived up to the 12th WAP while most of the legumes species died. Chromatographic profiles indicated 100% degradation of the oil fractions in C. cajan and L. purpureus after 90 days. For other legumes however, greater losses of crude oil fractions C₁–C₁₀ and C₁₀–C₂₀ were indicated in rhizosphere soil of P. vulgaris and G. max, respectively. The most effective removal (93.66%) of C₂₁–C₃₀ components was observed in G. max-planted soil even though vegetation was not established. The legumes especially C. cajan, L. purpureus and A. hypogea are promising candidates for phytoremediation of petroleum hydrocarbon-impacted soil.     

Bacterial consortium expressing surface displayed,intra- and extracellular lipases and pseudopyronine B for the degradation of oil

The lipA gene, encoding a solvent-tolerant extracellular lipase from Proteus sp. SW1, was displayed on the cell surface of Escherichia coli by fusing it to an antigen 43 anchoring motif. The display of LipA on the Escherichia coli cell surface was directly confirmed by immunofluorescence microscopy and flow cytometry. After 6 days of incubation in media containing 1 % used cooking oil, an Escherichia coli strain expressing surface displayed lipase was able to degrade 27 % of the oil. The biosurfactant, pseudopyronine B, was purified from culture supernatants of Pseudomonas sp. SL31. Its critical micelle concentration was determined to be 1400 mg/l, and the surfactant was stable within a temperature range from 0 to 120 °C and a pH range of 3–11. Pseudopyronine B-containing crude media extracts efficiently removed up to 51 % of the cadmium from contaminated water. We demonstrated the oil degradation ability of the mixed culture of four bacterial strains, namely the recombinant Escherichia coli expressing cell surface displayed lipase (pKKJlipA), His-tagged lipase (pETlipA), extracellular lipase-producing Proteus sp. SW1, and pseudopyronine B-producing Pseudomonas sp. SL31 by culturing in LB media containing 1 % oil. The consortium degraded 29 % of oil in one day and reached 84 % after 7 days.     

Soil bacterial strains with heavy metal resistance and high potential in degrading diesel oil and <Emphasis Type="Italic">n</Emphasis>-alkanes

Four bacterial strains, capable of degrading diesel oil, n-alkanes or hexadecane, were isolated from soils contaminated with petroleum oil and identified. Strains of Pseudomonas sp., Pseudomonas putida TPHK-1 and Pseudomonas aeruginosa TPHK-4, were more efficient in degrading high concentrations of the hydrocarbons than the other two strains, Stenotrophomonas maltophilia TPHK-2 and Acenitobacter sp. TPHK-3. P. putida TPHK-1 exhibited tolerance to very high concentrations of heavy metals such as cadmium, lead, zinc and copper. The innate ability of P. putida TPHK-1, as evidenced by the amplified genes alkB1 and alkB2 that encode alkane hydroxylases, and cat12o and cat23o coding for catechol dioxygenase, in degrading diesel oil in the presence of heavy metals is far greater than that of the strains reported in the literature. Heavy metal tolerance coupled with rapid degradation of hydrocarbons, even at high concentrations, suggests that P. putida TPHK-1 has a great potential in remediating soils contaminated with mixtures of hydrocarbons and heavy metals.     

Facies analysis of Pleistocene limestones from Neil West Coast Formation,Neil Island,Ritchie’s archipelago of South Andaman,India

Petrographic thin section analysis of the samples collected from the type section of Neil West Coast Formation, situated in the west coast of Neil Island yielded moderately preserved coralline red algae, benthic and planktic foraminifers, coral fragments, echinoid spines and gastropod shells. The coralline red algae are represented by both non-geniculate and geniculate forms. The non-geniculate forms belong to melobesids, lithophylloids and mastophoroides. The geniculate forms are represented by species of Amphiroa, Corallina, and Jania. However, the diversity and abundance of coralline algal forms are less in comparison to the benthic foraminifers those are represented by Amphistegina, Neorotalia, Ammonia, Elphidium, Operculina, Assilina, Amphisorus and texularids. Planktic foraminifers like Globigerinoides and other biogenic components viz., gastropod shells, echinoid spines and coral fragments are also common. A foraminiferal-algal grainstone facies has been recognized as observed in the field as well as in thin section analysis. The overall assemblage of the biogenic components and facies analysis indicate intertidal to near shore environment of deposition with high energy condition and increased hydrodynamic activity.     

Stability and phase relations of nonstoichiometric clinopyroxenes in the join diopside-Ca-Eskola component at high pressures

The stability of nonstoichiometric clinopyroxenes in the Di-CaEsk join was experimentally studied, and phase diagrams were constructed for this join at pressures of 2.0 and 3.0 GPa. It was found that melting in the diopside part of the join occurs at anomalously low temperatures, and nonstoichiometric clinopyroxene coexists with a phase approaching diopside in composition. Phase relations along the Di-CaEsk join can be described and consistently interpreted only assuming that the diopside phase (α-diopside) is thermodynamically stable. The following phase volumes were observed along the solidus of the join at a pressure of 3.0 GPa: Cpx, αDi+Cpx, αDi+Cpx+Qtz, αDi+Cpx+Grt+Qtz, Cpx+Grt+Qtz, Cpx+Grt+Ky+Qtz, Grt+Ky+Qtz. Melting occurs via the eutectic reaction αDi+Cpx+Grt+Qtz=L at a temperature of about 1200°C in the diopside part of the system and via the eutectic reaction Cpx+Grt+Ky+Qtz=L at a temperature of 1400°C in the calcium-rich part of the system. At a pressure of 2.0 GPa, melting occurs at temperatures of 1200–1300°C via the eutectic reaction αDi+Cpx+ An + Qtz=L. The invariant equilibrium (L, An, Cpx, Grt, αDi, Qtz) lies within the pressure range 2.0–3.0 GPa. Nonstoichiometric clinopyroxenes form complex solid solutions, the compositions of which are not strictly confined to the Di-CaEsk join and depend on temperature, pressure, and phase association. Grossular garnets coexist with nonstoichiometric clinopyroxenes and α-diopside.     

Community vulnerability to hazards: introducing local expert knowledge into the equation

The accuracies of three different evolutionary artificial neural network (ANN) approaches, ANN with genetic algorithm (ANN-GA), ANN with particle swarm optimization (ANN-PSO) and ANN with imperialist competitive algorithm (ANN-ICA), were compared in estimating groundwater levels (GWL) based on precipitation, evaporation and previous GWL data. The input combinations determined using auto-, partial auto- and cross-correlation analyses and tried for each model are: (i) GWL _t?1 and GWL _t?2; (ii) GWL _t?1, GWL _t?2 and P _t ; (iii) GWL _t?1, GWL _t?2 and E _t ; (iv) GWL _t?1, GWL _t?2, P _t and E _t ; (v) GWL _t?1, GWL _t?2 and P _t?1 where GWL _t , P _t and E _t indicate the GWL, precipitation and evaporation at time t, individually. The optimal ANN-GA, ANN-PSO and ANN-ICA models were obtained by trying various control parameters. The best accuracies of the ANN-GA, ANN-PSO and ANN-ICA models were obtained from input combination (i). The mean square error accuracies of the ANN-GA and ANN-ICA models were increased by 165 and 124% using ANN-PSO model. The results indicated that the ANN-PSO model performed better than the other models in modeling monthly groundwater levels.     

Paleoweathering and Paleoclimatic Reconstructions using Neoproterozoic Paleosol in the Eastern Margin of the Pranhita-Godavari Basin,India

Paleogene sediments of the inner fold belt, Naga hills, have very well preserved ichnofossils. 16 ichnospecies have been documented among 13 ichnogenera such as Arenicolites isp., Chondrites targionii, Cylindrichnus isp., Diplocraterion parallelum, Gyrochorte isp., Ophiomorpha annulata, O. irregulaire, O. nodosa, O. rudis, Palaeophycus tubularis, Planolites beverleyensis, Scolicia palaeobullia, Skolithos linearis, Trypinites weisei, Thalassinoides horizontalis and Zoophycos isp. The ichnofossil assemblages comprise mostly domichnia and fodinichnia benthos of the Skolithos and Cruziana ichnofacies. A shallow marine nearshore to offshore marine environment with fluctuating energy condition has been envisaged.     

Biostratigraphy and floristic evolution of coal swamp floras of a part of Talcher Basin,India: a window on a Permian temperate ecosystem

The present megafloral assemblage recorded from the Barakar sediments of Dholpahar section along Singda rivulet near Gopal Prasad Village in Talcher Basin comprises of equisetaceous stems, Gangamopteris buriadica, Palaeovittaria kurzii and 19 species of the genus Glossopteris. Record of Gangamopteris, Palaeovittaria and many narrow mesh forms of Glosspteris viz., G. angustifolia, G. churiensis, G. communis, G. recurva, G. spatulata, G. stenoneura, G. tenuifolia, G. vulgaris and G. zeilleri from two older fossiliferous horizons demonstrates that these fossils were preserved during Lower Barakar sedimentation. The report of middle and broad mesh forms of Glossopteris viz., G. barakarensis, G. browniana, G. indica, G. intermittens, G. karharbariensis, G. nakkarea, G. oldhamii, G. taeniensis and G. retifera in the youngest fossiliferous horizons reveals that these fossils were preserved during the deposition of Upper Barakar sediments. The continuation of some of the Karharbari plant fossils in the early phase of Barakar Formation and their disappearance in the flora of Late Barakar suggests a shift in the climatic setup. Palaeoclimate and palaeovegetation of this area are also summarised in this study. Moreover, the fossil assemblages of different fossiliferous beds of Dholpahar section demonstrate the evolution of midrib and meshes in different reticulate leaves.     

<Emphasis Type="Italic">Salpingoporella</Emphasis> (dasycladalean algae) species from the Lower Cretaceous carbonate facies of Kopet Dagh basin (NE Iran)

Salpingoporella species from algal bearing of Barremian-Aptian limestones in the Kopet Dagh basin (NE of Iran) are described. Different species (S. cemi, S. hasi, S. heraldica, S. hispanica, S. milovanovici, S. muehlmbergi, S. parapiriniae, S. piriniae, S. cf. biokovensis, S. steinhauseri, S. polygonalis) are investigated from different biometrical aspects such as depositional environments and biogeographical distribution as well as their systematic palaeontology from two formations (the Tirgan and Sarcheshmeh formations) in nine stratigraphic sections.     

Biostratigraphy of the Çeşmeler and Elmalι Dere formations: First records of upper Cretaceous planktonic foraminifera and radiolarians from the Eastern Pontides (Maçka,Trabzon, Turkey)

Upper Cretaceous pelagic deposits outcropping in the Maçka (Trabzon) region include radiolarians and pelagic foraminifera. The Çatak Group represented by the volcano-sedimentary successions consists of three formations having different properties. Two sections, ÇTK1 and ÇTK2, are selected from the Çe meler and Elmalι Dere formations, respectively, establishing the biostratigraphy of outcropping sedimentary units. A total of 17 species of Whiteinella, Helvetoglobotruncana, Marginotruncana, Dicarinella, Praeglobotruncana, Archaeoglobigerina and Hedbergella demonstrating the early Turonian–Coniacian are established in the ÇTK1 stratigraphic section. The early Turonian radiolarian fauna consisting of Halesium sexangulum Pessagno, 1971, Crucella cachensis Pessagno, 1971, Stichomitra communis Squinabol, 1903 is also defined in the same section. A total of 30 species of Crucella, Halesium, Pessagnobrachia, Patulibracchium, Alievium, Archaeospongoprunum, Dicyomitra, Stichomitra, Diacanthocapsa, Dactiyliodiscus, Amphipydax, Pseudoaulophocus, Acaeniotyle, Archaeodictyomitra, Actinomma, Xitus, Neosciadocapsidae characterizing the early and late Turonian, as well as the Coniacian–early Santonian are recognized from red-coloured pelagic limestones of the ÇTK2 section. Also, planktonic foraminifera species of Marginotruncana, Hedbergella, Heterohelix, Globotruncana, Globotruncanita, Archaeoglobigerina, Dicarinella characterizing the Coniacian–Santonian are described in the thin sections of the same samples. The age of red-coloured limestones is identified as the Coniacian–Santonian benefit from radiolarians and pelagic foraminifera. Consequently, radiolarians and pelagic foraminifera within sedimentary successions of the investigation area are distributed in two intervals that coincide with the early Turonian–Coniacian and Coniacian–Santonian intervals.     

The Bajocian–Bathonian plant fossil from the Hojedk formation,Lenjan section,Kerman, Iran

The Jurassic successions represent a wide distribution in North of the Kerman province. These successions include Ab-Haji, Badamu, and Hojedk formations. The Hojedk Formation contains the plant fossils. The Lenjan section is one of the suitable areas for paleontological studies on the Hojedk Formation. The study section is mostly composed of green sandstone and shale with several interbedded coal veins with different thicknesses. The thickness of the Hojedk Formation is about 200 m in the Lenjan section. In this study, seven genera and 13 species of macro plant fossils were identified and described, including Nilssonia undulata, Nilssonia bozorga, Nilssonia berriesi, Nilssonia sp., Klukia cf. exilis, Klukia exilis, Cladophlebis antarctica, Coniopteris lobata, Coniopteris murrayana, Elatocladus confertus, Podozamites sp., Equisetites sp., and Coniopteris sp. The Bajocian–Bathonian can be attributed to the Lenjan section based on the recognized flora.     

A new Jurassic cow shark (Chondrichthyes,Hexanchiformes) with comments on Jurassic hexanchiform systematics

A new taxon, ?Crassodontidanus gen. nov. of Hexanchiformes (cow sharks) from the Jurassic of Germany is described. It is characterized by peculiar teeth combining apomorphic (serrated mesial cutting edge of the main cusp) and plesiomorphic features (deep root with convex mesial and distal margins in labial and lingual views; protruding lingual root bulge). This character combination readily distinguishes members of the new taxon from all other known extant (Heptranchias, Hexanchus, Notorynchus) and extinct (?Gladioserratus, ?Notidanodon, ?Notidanoides, ?Pachyhexanchus, ?Paraheptranchias, ?Weltonia) hexanchiforms. Currently, two species, ?C. serratus (type species; Late Jurassic, Late Kimmeridgian of Nusplingen, South Germany) and ?C. wiedenrothi (Early Jurassic, Early Pliensbachian of Gretenberg (Hanover), North Germany) are assigned to this taxon. ?Crassodontidanus gen. nov. is member of ?Crassonotidae fam. nov. and sister to ?Notidanoides Maisey, 1986 and ?Pachyhexanchus Cappetta, 1990. We consider ?Notidanus amalthei Oppel, 1854 from the Pliensbachian of South Germany, ?Notidanus insignis Seguenza, 1887 from the Oxfordian of Sicily (Italy) and ?Notidanus wagneri Agassiz, 1843 from the Early Tithonian of Solnhofen (South Germany) nomina dubia and nomina nuda, respectively. The family ?Crassonotidae comprises plesiomorphic hexanchiforms ranging from the Sinemurian (Early Jurassic) to the Hauterivian (Early Cretaceous).     

The biostratigraphic and paleoecological importance of the subsurface Carboniferous microfossil assemblages from Faghur-1x well,Western Desert of Egypt

The microfossil assemblages of subsurface Carboniferous rocks from Faghur-1x were examined and identified. Their biostratigraphical and paleoenvironmental importance were investigated. The assemblage included well-preserved foraminifera like Omphalotis omphalota, Omphalotis sp. 2, Omphalotis sp. 3, Paraarchaediscus stilus, Paraarchaediscus koktjubensis, Archaediscus krestovnikovi, Archaediscus complanatus, Archaediscus inflatus, Archaediscus karreri, Diplosphearina inequalis, Eotubertina sp., Tetrataxis conica, Cribrostomum lecomptei, Palaeotextularia angulata, and Palaeotextularia longiseptata. This foraminiferal association indicates the late Viséan-early Serpukhovian. The other microfossils are gastropods, brachiopods, ostracods, crinoidal ossicles, frond-like fenestrate bryozoan types and stick-like colonies, echinoderms, microproblematica like Draffania biloba and algal Calcisphaera and the dasyclad Koninckopora. This microfossils assemblage points to the deposition in a restricted to open platform in a lagoonal framework environment. The Tehenu Basin is the eastern segment of northern African Sahara basins that provided refuge for the foraminiferal genera through the mass extinction events during the glacial Viséan-Serpukhovian times. However, its foraminiferal associations have lower diversities than the western basins, which indicate that it was more readily affected by the mass extinction event.     

Microbiostratigraphy of the Upper Paleocene to Middle Eocene Jahrum Formation in the Folded Zagros Zone,SW Iran

The Jahrum Formation (Upper Paleocene to Middle Eocene) is composed of carbonate and dolomitic carbonate rocks in the Zagros Basin. The Zagros is located at the boundary between the Arabian and Eurasian lithosphere plates and represent the orogenic response to a collision between Eurasia and advancing Arabia during the Cenozoic. The study area is located in the northern part of Kuh-E-Tudej, ~175 km southeast of Shiraz in the Folded Zagros Zone. The Jahrum Formation at Kuh-E-Tudej, with a thickness of 190 m, consists of medium to massive bedded limestone. The following foraminiferal index species are identified in the studied section: Fallotella alavensis, Kathina sp., Miscellanea sp., Lockhartia sp.,Orbitolites shirazeinsis, Nummulites sp., Opertorbitolites sp., Dictyoconus cf. egyptiensis, Orbitolites cf. complanatus, Dictyoconus sp., Coskinolina sp., Somalina stefaninii, Discocyclina sp., Praerhapydionina sp., Coskinolina cf. liburnica, Nummulites cf. globulus, Nummulites cf. aturicus, and Alveolina sp. The age of the studied sediments ranges from Upper Paleocene to Middle Eocene. The microbiostratigraphic studies revealed four biozones based on the foraminifers identified in the studied section.     

Analytical expressions for three-phase generalized relative permeabilities in water- and oil-wet capillary tubes

We analyze three-phase flow of immiscible fluids taking place within an elementary capillary tube with circular cross-section under water- and oil-wet conditions. We account explicitly for momentum transfer between the moving phases, which leads to the phenomenon of viscous coupling, by imposing continuity of velocity and shear stress at fluid-fluid interfaces. The macroscopic flow model which describes the system at the Darcy scale includes three-phase effective relative permeabilities, K _{i j,r} , accounting for the flux of the ith phase due to the presence of the jth phase. These effective parameters strongly depend on phase saturations, fluid viscosities, and wettability of the solid matrix. In the considered flow setting, K _{i j,r} reduce to a set of nine scalar quantities, K _{i j,r} . Our results show that K _{i j,r} of the wetting phase is a function only of the fluid phase own saturation. Otherwise, K _{i j,r} of the non-wetting phase depends on the saturation of all fluids in the system and on oil and water viscosities. Viscous coupling effects (encapsulated in K _{i j,r} with i ≠ j) can be significantly relevant in both water- and oil-wet systems. Wettability conditions influence oil flow at a rate that increases linearly with viscosity ratio between oil and water phases.     

The Influence of Oyster Farming on Sediment Bacterial Communities

Aquaculture currently provides half of all fish for human consumption, and this proportion is expected to increase to meet the growing global demand for protein. As aquaculture, including oyster farming, expands, it is increasingly important to understand effects on coastal ecosystems. The broad-scale ecological effects of oyster aquaculture are well documented; however, less is known regarding the influence of oyster aquaculture on sediment bacterial communities. To better understand this relationship, we compared three different oyster farming practices that varied in oyster biomass and proximity of oysters to the sediment. We used high-throughput sequencing and quantitative polymerase chain reaction to examine the effect of oyster farming on sediment bacterial communities. We examined the entire bacterial community and looked specifically at bacteria that support essential estuarine ecosystem services (denitrifiers), as well as bacteria that can be detrimental to human health (members of the Vibrio genus). We found that oyster biomass increased Vibrio richness and sediment carbon content, which influenced bacterial community composition. When compared to reference sites, the overall abundance of bacteria was increased by the bottom planting method, but the associated increases in denitrifiers and Vibrio were not significant. We were unable to detect V. parahaemolyticus, V. vulnificus, or V. cholera, the three most common Vibrio pathogens, in any sample, suggesting that oyster farming did not enhance these potential human pathogens in sediments at the time of sampling. These results highlight how differences in oyster farming practice can affect sediment bacterial communities, and the ecosystem services they provide.     

Early Triassic palynomorphs from Kuraloi Block,Belphar area,Ib-River coalfield,Mahanadi basin,Odisha

A rich assemblage of palynomorphs including miospores and megaspores has been recorded from the bore core no. IBKAN-2 drilled at a depth of 17.80m in Kuraloi Block-A, south-west part of the Ib-River Coalfield, Jharsuguda district, Odisha. The miofloral assemblage recovered shows prominence of cingulate, zonate, taeniate, non-striate and non saccate palynotaxa which indicate an early Triassic age and is comparable with the palynoassemblages of the same age known from the Damodar basin, India. The megaspore assemblage includes eight genera and fifteen species namely, Banksisporites karanpuraensis, B. indicus, B. utkalensis, Banksisporites sp., Barakarella pantii, Barakarella sp., Biharisporites spinosus, Biharisporites sp., Erlansonisporites triassicus, Erlansonisporites cf. erlansonii, Erlansonisporites sp. Jhariatriletes sp., Singhisporites sp., Ramispinatispora sp. and Talchirella trivedii. Records of megaspores are sparse in the early Triassic of Gondwana basins of India. The present study records the first occurrence of miospores and megaspores from the early Triassic of Mahanadi basin besides substantiating the earlier data.     

Food Habits of Large Nektonic Fishes: Trophic Linkages in Delaware Bay and the Adjacent Ocean

Fish diets play a critical role in our understanding of aquatic trophic dynamics and are an important component in developing ecosystem-based approaches to fisheries management. Although large nektonic fishes exert top-down predator effects on the food web and typically support viable commercial and recreational fisheries, little is known about the diet of this guild. We evaluated the diets (6327 stomachs) of four nektonic predatory fishes (Pomatomus saltatrix [78–395 mm], Cynoscion regalis [91–520 mm], Morone americana [156–361 mm], and Morone saxatilis [82–785 mm]) in Delaware Bay and in the adjacent ocean. To assess ontogenetic, geographic, and interspecific variation in diets, observations from individual fish stomachs were clustered into species-size class groups, and dietary overlap was estimated using multivariate analyses. A shift in diet composition, as well as diversity, occurred along the estuarine gradient and into the adjacent ocean. Some prey were shared by most predators, including some crustaceans (dominated by Callinectes sapidus, mysids, and Palaemonetes spp.), fundulids (dominated by Fundulus heteroclitus), engraulids (dominated by Anchoa mitchilli), and clupeids (dominated by Brevoortia tyrannus). However, inter- and intra-specific variation in diet was observed as well. In particular, M. americana consumed fewer engraulids and clupeids, and many more and diverse types of invertebrates, while P. saltatrix consumed more clupeids and less invertebrates. The lack of overlap in diet between the four predators evaluated, and between size groups for each predator, supports previous evidence that these groups feed in trophic guilds defined by species and by size within a species. The highly variable diets for these predators suggest high resolution spatial data are necessary in order to quantify their most important prey and their role in coastal ecosystems.     

The fish fauna of the Late Jurassic Solothurn Turtle Limestone (NW Switzerland)

The surroundings of Solothurn (NW Switzerland) have been known for their fossil marine turtles since the beginning of the nineteenth century. In more recent history, access to the fossil bearing layers i.e. the Rätschenbank (Kimmeridgian, Late Jurassic) has not been possible until 1986 when one of the old quarries in the area re-opened. A series of excavations from 1986 to 1989 provided new material and a unique opportunity to re-investigate the fish fauna of the Solothurn Turtle Limestone that has not been dealt with since Agassiz (1833–1844). Examination and classification of the Solothurn specimens, mainly jaw fragments and teeth, furnished 11 species in 6 major groups: chimaeras (Ischyodus), selachians (Hybodus, Paracestracion, Asteracanthus), semionotids (Lepidotes), pycnodontids (Gyrodus, Proscinetes), caturids and oligopleurids (Caturus, Callopterus, Ionoscopus), and aspidorhynchids (Belonostomus).