Assessment of the ecological quality status of soft-bottoms in Reunion Island (tropical Southwest Indian Ocean) using AZTI marine biotic indices

The ability of the two synthetic marine biotic indices, AMBI and M-AMBI, to account for changes in the ecological quality of coastal soft bottoms of Reunion Island according to disturbances was assessed from macrobenthic samples collected in five sectors between 1994 and 2004. Samples were collected under non-perturbed conditions and at two sites subjected to heavy organic enrichment. Both indices are based on a classification of macrofauna into ecological groups (EG), and their transfer to tropical waters required some adaptations. These indices proved efficient in detecting a degradation of habitat quality. Their use resulted in the classification of all sites sampled between 1996 and 1998 as "good" or "high". M-AMBI nevertheless tended to result in the attribution of a slightly worse ecological quality status than AMBI. Together with an update of the EG species list for the Indian Ocean area, our results support the extension of both indices for the assessment of tropical soft bottoms.     

An insight into the performance of benthic classification indices tested in Eastern Mediterranean coastal waters

This work evaluates four indices that have been used for benthic macroinvertebrate ecological quality classification in the Mediterranean Sea. Our study was based on the data obtained from the participation of Greece and Cyprus in the Mediterranean Geographical Intercalibration group. The indices AMBI, M-AMBI, MEDOCC and BENTIX were applied to the available benthic species data, and the succession of the ecological groups along the graded values of each index was plotted. The level of agreement among methods was calculated, and the performance of each method in estimating ecological quality status was evaluated. AMBI, based on the Atlantic model, and its derivative M-AMBI overestimated the statuses, while MEDOCC showed the best level of agreement with BENTIX. BENTIX gives equal weight to tolerant and opportunistic species groups, which correlates them more closely than the other indices and makes BENTIX the most sensitive in detecting ecological disturbances in the Eastern Mediterranean basin, where tolerant and opportunistic groups seem to play an equally important role in the response of benthic communities to stressors.     

Towards the application of the Water Framework Directive in Italy: Assessing the potential of benthic tools in Adriatic coastal transitional ecosystems

The potential of four benthic indices (AMBI/M-AMBI, BENTIX, BITS) was assessed in Italian coastal transitional ecosystems. The community composition showed a strong dominance of lagoonal, tolerant species, and out of more of 400 species found, only about 40 taxa were dominant. The full agreement of the four indices on an undegraded (Good or better) or degraded (Moderate or worse) status occurred only in 32.3% of stations. This study evidenced that BENTIX is inappropriate for eutrophic Adriatic lagoons, and that in such environments M-AMBI classification is actually too much dependent on diversity and richness, and seems unable to capture some peculiarities of benthic assemblages in transitional waters. AMBI and BITS gave similar classifications despite the different level of taxonomic identification needed. The unmodified use of these indices might impair accurate assessment of ecological quality status and decision-making on the managers’ point of view.     

Characterization of the ecological quality of the coastal Gulf of Lions (NW Mediterranean). A comparative approach based on three biotic indices

The ecological quality of the Gulf of Lions coast was assessed using three biotic indices (H', AMBI and BQI). The three indices correlated positively. The positive correlation between AMBI and BQI was surprising and was mostly due to the fact that the dominant species Ditrupa arietina featured a low ES50(0.05) but was classified in GI by AMBI. Both H' and BQI were efficient in distinguishing impacted from un-impacted sites but AMBI was not. Differences between H' and BQI were mainly due to the scale used to translate indices in terms of EcoQ. The three indices were able to detect the major changes in macrofauna composition, which occurred in the Bay of Banyuls-sur-Mer during the last 40years. However, the interpretations of such changes in terms of EcoQ differed between indices. These results are discussed relative to the characteristics of the tested indices.     

Benthic indicators: analysis of the threshold values of ecological quality classifications for transitional waters

The effect of the modifications of the threshold values generally adopted for the five EcoQ (Ecological Quality) classes recognized by the European Water Framework Directive (WFD) for the most used benthic indicators and diversity indicators (AMBI, BENTIX, BOPA, BQI, H', ITI and M-AMBI) were studied to test whether it is possible to obtain the same or similar ecological classifications for transitional waters using these different indicators, and determine whether the current classifications can be improved. The analysis included: (i) the use of indicator equations, (ii) the use of reference indicators, and (iii) the use of indicator distribution laws. These investigations demonstrated that it was impossible to obtain an exact intercalibration of the ecological classifications with the selected indicators. However, some propositions to improve the actual classification were suggested.     

Benthic biodiversity indices versus salinity gradient in the southern Baltic Sea

The need to assess the environmental status of marine and coastal waters according to the EU Water Framework Directive (WFD) encouraged the design of specific biotic indices to evaluate the response of benthic communities to human-induced changes in water quality. In the present study three of these indices, the traditional Shannon Wiener Index (H') and the more recently published AMBI (AZTI' Marine Biotic Index) and BQI (Benthic Quality Index), were tested along a salinity gradient in the southern Baltic Sea. The comparison of the three indices demonstrates that in the southern Baltic Sea the ecological quality (EcoQ) classification based on macrozoobenthic communities as indicator greatly depends on the biotic index chosen. We found a significant positive relation between species number, H', BQI and salinity resulting in EcoQ status of "Bad", "Poor" or "Moderate" in areas with a salinity value below 10 psu. The AMBI was less dependent on salinity but appear to partly overestimate the EcoQ status. Presently none of these biotic indices appear to be adjusted for application in a gradient system as given in the southern Baltic Sea. A potential approach describing how to overcome this limitation is discussed.     

Exploring the marine biotic index (AMBI): variations on a theme by Ángel Borja

The results of manipulating the input data to AMBI are explored using various transformations of numerical species abundance (NAMBI), biomass (BAMBI) and production (PAMBI) from a variety of stations on the NE Atlantic shelf at which the pollution/disturbance status is known. There is a close agreement between the proportions of species in the five AMBI ecological groups and a phylum level meta-analysis axis of increasing environmental impact. All AMBI measures provide a better monotonic relationship with the impact axis than do traditional species diversity measures, which show higher diversity at intermediate levels of disturbance. A marginally better relationship with the impact axis of the meta-analysis is achieved by a moderate (square root) transformation of the data. ‘Production’ data (an appropriate combination of abundance and biomass information) provide only a marginal improvement on abundance data, but are ecologically and functionally much more relevant. Severe transformation of the data, culminating in presence/absence, degrades the relationship with the impact axis, but if only simple species lists are available then these may still be useable in making an environmental assessment.     

Spatio-temporal changes of marine macrobenthic community in sub-tropical waters upon recovery from eutrophication. II. Life-history traits and feeding guilds of polychaete community

A two-year study was conducted in the vicinity of a harbour in sub-tropical Hong Kong, to examine the progress of recovery of macrobenthic community, based on analyses of both life-history traits and trophic guilds of polychaetes, upon cessation of organic pollution caused by sewage discharge. Seventy seven out of 83 species collected were classified under four ecological groups based on the life-history traits and sensitivity to organic gradients. The mean ATZI marine biotic index (AMBI) derived from these ecological groups showed spatial difference among the five sampling locations. In particular, the presence of different percentages of polychaete species from Groups III (unbalanced community) and IV (polluted community) suggested the presence of pollution stress in certain degree at all sampling locations. However, no significant temporal changes were noted over the study period. From all polychaete species identified, they were classified into 13 feeding guilds. The mean diversity of these feeding guilds at most of the sampling locations was significantly higher than that at one of the inside-harbour locations. The composition of feeding guilds was also significantly different spatially. At one of the inside-harbour locations, the dominant feeding guilds were motile/discretely motile surface deposit feeders with tentaculates or unarmed pharynx, and motile omnivores with jawed pharynx in the first year of study, but were replaced by motile, jawed carnivores in the second year of study. The increased proportion of carnivores over the study period can be seen as a sign of recovery in the community structure since abundance of predators is commonly higher in habitats with better environmental quality. The implications of using life-history traits and feeding guild analyses for benthic community are discussed.     

Long-term trends in benthic habitat quality as determined by Multivariate AMBI and Infaunal Quality Index in relation to natural variability: a case study in Kinsale Harbour, south coast of Ireland

Benthic Ecological Quality Ratios (EQR) are important tools for assessing the ecological status of coastal and transitional water bodies. Here, we use spatial and time-series data from Kinsale Harbour, Ireland to examine the effects of sample processing methodologies on the outputs of two EQRs: Multivariate AMBI (M-AMBI) and Infaunal Quality Index (IQI).Both EQRs were robust to changes in sieve size from 1 mm to 0.5 mm, and to changes in the taxa identified in spatial calibration. Both EQRs classified habitat quality in Kinsale as generally Good or High with no evidence of significant change over the time series (1981-2006). IQI classified the ecological status as higher than M-AMBI.There was a significant relationship between IQI and M-AMBI in spatial calibration, but no significant relationship between them in time series. Further research into the behaviour of EQRs in relation to natural variability over long time-scales is needed to discriminate anthropogenic impacts reliably.     

Ecological quality status of coastal benthic ecosystems in the Sea of Marmara

The macrofauna in the north Marmara Sea were identified and evaluated with univariate measures and biotic indices (BENTIX, AMBI). As expected, the diversity and species' variety decreased as the sediments became muddier and as the stress increased. The AMBI index failed to detect the spatial differentiation of the EcoQ between the sites whereas, the BENTIX index succeeded (in 70% of the cases) in producing an ecologically relevant classification reflecting the environmental pressures. Results suggested that risks of reduced species' richness and diversity from organic loading and other associated stressors in sediments should be relatively low at TOC concentrations < about 6 mg/g, high at concentrations>about 22 mg/g, and intermediate at concentrations in-between. It is suggested that these TOC critical points may be used as a general screening-level indicator for assessing the EcoQ in association with reduced biodiversity over coastal areas receiving organic wastes and other pollutants from human activities. It is further suggested that both assignment of species to ecological groups and classification scales for biotic indices (AMBI, BENTIX) for sites in the Mediterranean Sea should be readjusted so as to be closer related to environmental variables.     

Distribution patterns of macrobenthic species in relation to organic enrichment within aquaculture earthen ponds

The relationship between organic enrichment and macrobenthic colonization patterns was investigated during an 8-month period in Diplodus sargus (white seabream) production ponds. A stratified sampling design was applied and each pond was divided into three zones: water entrance (WE); central (C); and automatic feeder zones (AF). Generally, the number of species and Shannon–Wiener diversity increased from the WE to the AF zone. Abundance did not present a clear trend. The recently developed marine biotic index (AMBI) was applied and showed to be sufficiently robust to discriminate, within a relatively small area, differences in macrobenthic communities due to organic enrichment. Nevertheless, caution is advised when applying this index or others based on ecological group’s assignment, as the classification of a certain area may differ when allocating a certain species to an unsuitable group. This is particularly evident when common species are involved.     

Functions and ecological status of eight Italian lagoons examined using biological traits analysis (BTA)

The soft-bottom communities of eight Italian lagoons were analyzed for eight biological traits (feeding, mobility, adult life habitat, body size, life span, reproductive technique, type of larva and reproductive frequency) in order to identify the dominant traits in different transitional environments. We considered the ecological quality status (EcoQS) of the stations, assessed by two biotic indices, AMBI and Bentix. Stations were categorized into EcoQS classes to investigate the relationship between biological functions and ecological quality. The results indicate that the variability of the data was governed by traits linked to resource utilization rather than to life cycle. Lagoons affected by chronic disturbance displayed a poor functional composition, which usually corresponded to poor EcoQS in some cases, correlations between ecological groups and traits modalities were ecologically relevant; however, classes of EcoQS were found to be relatively independent from the functional structure of the considered stations.     

Seasonal variability of benthic indices: an approach to test the applicability of different indices for ecosystem quality assessment

In recent studies, several benthic biological indices were developed or used to assess the ecological quality status of marine environments. In the present study the seasonal variability of several univariate and multimetric indices was studied on a monthly scale (September 2000 until May 2002) in different areas of the North Sea such as the German Bight, the Oyster Ground and the Dogger Bank. The stations were chosen to reflect a gradient in the hydrographic regime, temperature and organic matter supply. The seasonal variability was highest for the univariate indices such as the Shannon–Wiener Index and the Hurlbert Index. Thus, due to sensitivity to recruitment the corresponding ecological status ranged from ‘good’ to ‘poor’ depending on the season. For the multimetric indices such as the AMBI or the BQI the seasonal variability and the corresponding ecological status were low. The results are discussed concerning possible consequences for ecological quality assessment especially related to the Water Framework Directive (WFD).     

Performance comparison of two biotic indices measuring the ecological status of water bodies in the Southern Baltic and Gulf of Lions

Two biotic indices, ATZI Marine Biotic Index (AMBI) and Benthic Quality Index (BQI) have been recently introduced within the EC Water Framework Directive to assess the quality of marine habitats: both are based on sensitivity/tolerance classification and quantitative information on the composition of soft-bottom macrofauna. Their performance, especially with regard to sampling effort was assessed based on two data sets collected in Southern Baltic and one from the Gulf of Lions Mediterranean. AMBI was not affected by sampling effort but BQI was. Two modifications were proposed for BQI (i.e., BQI) (1) the removal of the scaling term (i.e., BQI(W)), and (2) the replacement of the scaling term by different scaling term (i.e., BQI(ES)). Both modified BQIs were largely independent of sampling effort. Variability was slightly lower for BQI(W) than for BQI(ES). BQI was highly correlated with BQI(W) and with BQI(ES) both in the Southern Baltic and in the Gulf of Lions. However, the proportions of stations, which were not attributed the same ecological quality status (EcoQ) when using BQI and its two modified forms were always high. Differences in ecological classification were mostly due to the scales used to infer EcoQ. Based on this study we recommend to use BQI(ES) in future studies because it apparently constitutes the best compromise in (1) being independent of sampling effort, (2) limiting the variability in computation in relation with sampling effort, (3) being correlated with BQI and corresponding EcoQ.     

An intercalibration of classification metrics of benthic macroinvertebrates in coastal and transitional ecosystems of the Eastern Mediterranean ecoregion (Greece)

Three coastal and three lagoonal sites located in Greece (Eastern Mediterranean) were selected to test and intercalibrate classification methods developed for benthic invertebrates ecological quality assessments. These methods were developed for the purposes of the European Water Framework Directive which is a European legislation adopted for the protection and improvement of the coastal and transitional waters. However, through testing these methods, this work addresses in general the issue of determining and comparing the ecological status of the coastal and transitional macrobenthic communities in Eastern Mediterranean ecosystems. Among methods tested were the biotic indices AMBI and BENTIX, the multivariate method M-AMBI and the biomass size structure index ISD in lagoonal sites only. ISD index is a rather new method developed especially for transitional ecosystems and in this contribution its performance is intercalibrated with the other benthic classification metrics. Comparison and intercalibration of the indices results in assessing the ecological quality status (EQS) are presented graphically and statistically performing the Kappa analysis. Results of the comparison and the indices' performance are evaluated based on the knowledge of the sites' environmental condition and baseline studies. Based on an extended dataset of coastal and transitional ecosystems benthic invertebrates, an evaluation of each index performance and effectiveness is attempted through an insight and comparative analysis of each methods' structure and design. Results showed that in these Eastern Mediterranean coastal sites the BENTIX index seems to give a more biologically relevant classification and gives a higher confidence level regarding the classification compared to the other indices. In the coastal areas AMBI showed a tendency for the "good" class classification, while the factorial M-AMBI gave more consistent results with the BENTIX. In the lagoonal sites the biotic indices tested were not proved efficient enough and the biomass based ISD index proved more appropriate for these ecosystems. AMBI showed the highest agreement with the ISD which is due to the better performance of this index in the slightly and moderately polluted lagoons, compared to the other indices. However, AMBI failed to classify the polluted lagoon, in which case the BENTIX gave a more relevant classification.     

Influence of seasonal variability in benthic invertebrate community structure on the use of biotic indices to assess the ecological status of a Portuguese estuary

The present study focused on the use of benthic invertebrate communities to assess the ecological quality of a Portuguese estuary characterized by strong seasonal changes and with eutrophication problems. Seasonal benthic samples were collected during a flood year and the methodology proposed by the WFD Portuguese group was used to classify benthic assemblages into five different quality classes. Factor analysis was applied to classify stations based on their physical-chemical status. Different classifications were obtained with different indices and among seasons and there was low agreement between indices and index-season interactions. Diversity indices were better correlated to eutrophication related variables than AMBI and ABC method. Predictable responses of benthic indices to anthropogenic stress symptoms were stronger during the dry period.     

The performance of benthic indicators of ecological change in Adriatic coastal lagoons: throwing the baby with the water?

The ecological quality of 127 stations from six Adriatic coastal lagoons was assessed using a suite of biotic indices: H', d, 1-lambda', Delta(*), Delta(+), W, AMBI, BOPA and FINE. The analysis indicated the difficulties in deriving and using the existing indices from benthic communities in highly variable environmental conditions in coastal lagoons. Different metrics rendered different results: the use of H' resulted in the classification of all stations as "Moderate/Poor/Bad", whereas BOPA classified the majority of the stations as "Good/High". Using Delta(+), most of the stations resulted as "Not anthropogenically impacted", while the W-statistics gave 82 stations as "Undisturbed" and 45 as "Moderately/Grossly disturbed". AMBI classified 55 stations as "High/Good" and 72 as "Moderate/Poor/Bad", whereas those were 32 and 95 with FINE, respectively. The latter, which was developed just for Italian coastal lagoons, was the most conservative among the indices studied. Yet, it showed the highest correlation with the species/abundance matrix. Using indices developed for coastal waters in lagoons can give the distorted indication that the water body is degraded, when it might be just a natural, low diversity, high abundance community, i.e. a lagoonal community. Moreover, the outcome of the use of a certain index has a financial dimension such that lagoons misclassified as being "poor status" will then require expensive remediation measures. For the lagoons studied, there is probably an adequate quality and quantity of benthic data available for making management decisions, but this study highlights the limits of the existing indices for lagoonal ecosystems. The challenge for the next future is to couple long term conservation of the natural environment with the highly productive activities carried out in lagoonal ecosystems.     

Is it possible to assess the ecological status of highly stressed natural estuarine environments using macroinvertebrates indices?

Several biotic indices have been proposed for the assessment of the ecological status of benthic macroinvertebrates in marine waters, although none have been generally accepted. When it comes to assessing highly stressed natural environments, such as estuaries, the controversy and uncertainty is much higher than for any given normal index. In this article, we test the behavior and suitability of different biotic indices proposed under the perspective of the Water Framework Directive (S, H, AMBI, M-AMBI, BQI, W-statistic, Taxonomic distinctness) for the assessment of estuaries in northern Spain. The low species richness and dominance of a few tolerant species in the characteristic community of these estuaries presented a challenge to the application of the biotic indices tested. Combined approaches that integrate different aspects of water quality and ecosystem functionality could increase the reliability of the ecological assessment of these transitional waters.     

Response of single benthic metrics and multi-metric methods to anthropogenic pressure gradients, in five distinct European coastal and transitional ecosystems

In recent times many benthic indices have been proposed to assess the ecological quality of marine waters worldwide. In this study we compared single metrics and multi-metric methods to assess coastal and transitional benthic status along human pressure gradients in five distinct environments across Europe: Varna bay and lake (Bulgaria), Lesina lagoon (Italy), Mondego estuary (Portugal), Basque coast (Spain) and Oslofjord (Norway). Hence, 13 single metrics (abundance, number of taxa, and several diversity and sensitivity indices) and eight of the most common indices used within the European Water Framework Directive (WFD) for benthic assessment were selected: index of size spectra (ISS), Benthic assessment tool (BAT), Norwegian quality index (NQI), Multivariate AMBI (M-AMBI), Benthic quality index (BQI), (Benthic ecosystem quality index (BEQI), Benthic index based on taxonomic sufficiency (BITS), and infaunal quality index (IQI). Within each system, sampling sites were ordered in an increasing pressure gradient according to a preliminary classification based on professional judgement. The different indices are largely consistent in their response to pressure gradient, except in some particular cases (i.e. BITS, in all cases, or ISS when a low number of individuals is present). Inconsistencies between indicator responses were most pronounced in transitional waters (i.e. IQI, BEQI), highlighting the difficulties of the generic application of indicators to all marine, estuarine and lagoonal environments. However, some of the single (i.e. ecological groups approach, diversity, richness) and multi-metric methods (i.e. BAT, M-AMBI, NQI) were able to detect such gradients both in transitional and coastal environments, being these multi-metric methods more consistent in the detection than single indices. This study highlights the importance of survey design and good reference conditions for some indicators. The agreement observed between different methodologies and their ability to detect quality trends across distinct environments constitutes a promising result for the implementation of the WFD’s monitoring plans. Moreover, these results have management implications, regarding the dangers of misclassification, uncertainty in the assessment, use of conflicting indices, and testing and validation of indices.