Seasonal patterns of benthic arthropods in a subtropical Andean basin

We studied the seasonal variation of arthropod assemblages and the response to high discharge events in a subtropical Andean basin. Using abundance of common taxa and taxon richness of each sampled site, we examined the temporal variation of arthropods and related these changes with 12 environmental variables. Seasonal patterns of benthic arthropods were confirmed by uni- and multivariate techniques. Benthic abundance and taxon richness peaked on autumn-winter months, and declined abruptly on the month of peak discharge (summer season). Distinct taxonomic groups were dominant in each season influenced by discharge and seven chemical variables.Assemblage resistance to disturbance by spates was low. Rapid recovery of benthic arthropods after floods reflected high resilience of the system. The dominance of groups of short life cycles such as Ephemeroptera played an important role in this process.     

Glacial flood pulse effects on benthic fauna in equatorial high‐Andean streams

Equatorial glacier‐fed streams present unique hydraulic patterns when compared to glacier‐fed observed in temperate regions as the main variability in discharge occurs on a daily basis. To assess how benthic fauna respond to these specific hydraulic conditions, we investigated the relationships between flow regime, hydraulic conditions (boundary Reynolds number, Re*), and macroinvertebrate communities (taxon richness and abundance) in a tropical glacier‐fed stream located in the high Ecuadorian Andes (> 4000 m). Both physical and biotic variables were measured under four discharge conditions (base‐flow and glacial flood pulses of various intensities), at 30 random points, in two sites whose hydraulic conditions were representative to those found in other streams of the study catchment. While daily glacial flood pulses significantly increased hydraulic stress in the benthic habitats (appearance of Re* > 2000), low stress areas still persisted even during extreme flood events (Re* < 500). In contrast to previous research in temperate glacier‐fed streams, taxon richness and abundance were not significantly affected by changes in hydraulic conditions induced by daily glacial flood pulses. However, we found that a few rare taxa, in particular rare ones, preferentially occurred in highly stressed hydraulic habitats. Monte‐Carlo simulations of benthic communities under glacial flood reduction scenarios predicted that taxon richness would be significantly reduced by the loss of high hydraulic stress habitats following glacier shrinking. This pioneer study on the relationship between hydraulic conditions and benthic diversity in an equatorial glacial stream evidenced unknown effects of climate change on singular yet endangered aquatic systems. Copyright © 2013 John Wiley & Sons, Ltd.     

Do installed stream logjams change benthic community structure?

Installed logjams constructed of wood are commonly used in stream restoration projects to provide habitat for lotic organisms. Macroinvertebrate densities are known to increase on logjam surfaces; however, less is known about the influence logjams have on benthic organisms inhabiting the surrounding streambed. To examine this, we conducted a before-after-control-impact (BACI) study in a stream in northern Minnesota, USA, to determine how an installed logjam affected the richness and abundance of three insect orders commonly used in biotic indices, the Ephemeroptera, Plecoptera, and Trichoptera (i.e., EPT taxa). A spanning logjam composed of three logs bound together was installed perpendicular to stream flow at the impact site. Initial sampling of the impact site and an upstream control found no differences among the ETP taxa. A year after installation, the logjam accumulated woody debris and altered flow so that near-bed current at the impact site was faster and more heterogeneous than at the control site. Although the richness and abundance of the macroinvertebrate community as a whole did not differ between sites after one year, it did for the Ephemeroptera and Plecoptera at the impact site. By contrast, Trichoptera richness and abundance did not change. Our results lend support to the hypothesis that installed logjams may enhance stream habitat not only by providing colonization surfaces for macroinverbrates, but also by altering the benthic environment of the surrounding habitat.     

Benthic and hyporheic invertebrate assemblages along a gradient of increasing streambed colmation by fine sediment

Streambed colmation by fine sediment, e.g. the deposition, accumulation and storage of fines in the substrate, is a major environmental concern throughout the world. Nevertheless, the ecological effects of streambed colmation on both benthic and hyporheic invertebrate assemblages have rarely been considered simultaneously. We studied a continuum of a naturally increasing percentage of fine sediment in three temperate rivers and hypothesized that the increasing percentage of fine sediment would decrease both benthic and hyporheic invertebrate densities and diversities, and reduce the similarities between them. To test these hypotheses, we first compared heavily, moderately and lightly clogged reaches located in downwelling areas and sampled invertebrates in the benthic zone and at 3 different depths (10, 30 and 50 cm) in the hyporheic zone. Secondly, we modified the sediment grain size distribution experimentally by increasing the percentage of fine sediment and using artificial substrates. The increasing colmation halved the hyporheic taxonomic richness and reduced benthic and hyporheic densities to a third. Some taxa were found in both zones, mainly in high colmation (e.g. Baetidae) or low colmation contexts (e.g. Orthocladiinae, Cyclopoida and Harpacticoida). The dissimilarity between benthic and hyporheic fauna (only at ?50 cm) was significantly higher in heavily clogged reaches than in moderately and lightly clogged ones, suggesting reduced vertical exchange of invertebrates or differential impacts between zones. The total abundance, taxonomic richness, percentage of EPT taxa and densities of most organisms observed using the artificial substrates decreased linearly with the increasing percentage of fine sediment in the experiment. Only the Ephemeroptera Caenis spp. and Heptageniidae disappeared above 30 and 50 % of fine sediment, respectively, suggesting that the response to increasing colmation are strongly taxon-specific. High amount of fine sediments within the substrate significantly decreased habitat quality for benthic and hyporheic invertebrates and thus limit the production of streams and their capacity to recover after disturbance. Moreover, the use of hyporheic invertebrates seems more relevant than benthic invertebrates to assess the effect of colmation and thus could be tested in future research as indicators.     

Effects of human disturbance and riparian conditions on Odonata (Insecta) assemblages in eastern Amazon basin streams

Riparian vegetation is an important determinant of the physical, chemical, and biological condition of streams, and odonates are useful indicators of riparian condition. To identify environmental factors that structure Odonata assemblages in tropical forest streams, we collected adult odonate specimens and habitat data from 50 stream sites located in the Brazilian municipality of Paragominas (Pará state). We collected 1769 specimens representing 11 families, 41 genera, and 97 species. Of these species, 56 were Zygoptera, and 41 were Anisoptera. Improved environmental condition was reflected in increased Zygoptera species richness and reduced Anisoptera species richness. Channel shading was strongly and positively related to Zygoptera richness, and negatively to Anisoptera richness. Zygoptera species richness, but not Anisoptera species richness, was related positively to bank angle, quantity of wood in the stream bed, electrical conductivity, and decreased water temperature. Altered riparian vegetation structure was the principal determinant of odonate assemblage structure. Our results indicate that maintaining intact riparian vegetation is fundamental for conserving or re-establishing aquatic odonate assemblage structure.     

Do seasonal changes in habitat features influence aquatic macroinvertebrate assemblages in perennial versus temporary Mediterranean streams?

In this study we examined the importance of seasonal changes in habitat features and aquatic macroinvertebrate responses in temporary and perennial streams from two different catchments in the Western Mediterranean region in Spain. Macroinvertebrate sampling was spatially intensive to account for the relative frequency of meso- (i.e., riffles and pools) and micro-habitats (i.e., different mineral and organic-based substrata) at each site. Samples were collected at two distinctly different phases of the hydrograph: (1) during the flowing period, when pool-riffle sequences were well-established, and (2) during the dry phase, when only isolated pools were expected to occur in the temporary streams. During the dry season, both a reduction in the available total habitat and in microhabitat diversity in all sites studied was observed. As a result, taxon richness decreased in all streams, but more dramatically at temporary stream sites and particularly so in the infrequently remaining discontinuous riffles. Macroinvertebrate assemblages differed among catchments (i.e., geographical identity) and sites (perennial vs. temporary). Invertebrate differences were also strong within and among meso- and micro-habitats, particularly mineral and organic microhabitat patches, and differences were due to both loss of taxa from some habitats and some taxa exhibiting certain habitat affinities.     

Effects of fluvial processes and human activities on stream macro-invertebrates

Benthic macro-invertebrates are vital components of river ecosystems.The effects of fluvial processes and human activities on the distribution of macro-invertebrates were studied through field investigations and experiment.Sixty-one sampling sites on 31 rivers in China were selected to investigate the structures of macro-invertebrate assemblages.The rivers,according to their fluvial conditions,are classified as streams with a stable channel bed,degrading channel bed,aggrading channel bed,and intensive bed load motion.The structures of macro-invertebrate fauna for the four types of rivers are very different.Stable rivers have a large number of individuals,abundant fauna, and high biodiversity;while the density and taxa richness for degrading rivers are small,and those for aggrading rivers are much less;whereas the ecology of rivers with intensive bed load motion are the worst.This paper proposes that streambed stability is the primary influential factor shaping the structure of benthic macro-invertebrate communities.Organic pollution can obviously result in the decrease of biodiversity,in the simplification of macro-invertebrate structures,and in the distortion of functional feeding group composition.In a river with high total nitrogen content,the relative abundance of collector-gatherers is high,and that of collector-filterers,scrapers,shredders,and predators are low.Scrapers,shredders,and predators disappear in severely polluted rivers.The isolation of aquatic habitat results in a distinct decrease of individual numbers and taxa richness.This result demonstrates that the connectivity of aquatic habitat significantly affects macro-invertebrate assemblages.A practical method to calculate a Habitat Suitability Index(HSI) is proposed,integrating the effects of the primary physical(including biotic and abiotic) and chemical factors.The biodiversity and taxa richness increase non-linearly with HSI.     

Invertebrate colonization of leaves and roots within sediments of intermittent Coastal Plain streams across hydrologic phases

Ecological flows between habitats are vital for predicting and understanding structure and function of recipient systems. Ecological flows across riparian areas and headwater intermittent streams are likely to be especially important in many river networks because of the shear extent of these interfaces, their high edge-to-width ratio, and the alternation of wet and dry conditions in intermittent channels. While there has been substantial research supporting the importance of riparian-stream linkages above-ground, comparatively less research has investigated below-ground linkages. We tested the hypothesis that riparian roots are colonized by invertebrates as a food source within stream beds of intermittent headwater streams. We compared benthic invertebrate assemblages colonizing three types of buried substrates (leaves, roots, and plastic roots) among three intermittent Coastal Plain streams, each with a different riparian management treatment (clearcut, thinned, and reference), over a 1-year period. Invertebrate density was significantly lower in root litterbags than in plastic roots litterbags, but neither differed from densities in leaf litterbags. Total invertebrate abundances, however, were significantly higher in leaf and root litterbags compared to abundances in plastic root litterbags. Invertebrate biomass and richness did not vary among substrates, but invertebrate density, abundance, and richness all declined from the wet phase (September–December) through the dry phase (June–August). Meiofauna and aquatic dipterans were the primary colonizing invertebrates during the wet phase. Relative abundance of terrestrial taxa increased during the dry phase, but their absolute abundance remained lower than aquatic taxa during the wet phase. Invertebrate composition did not differ among substrate types, but was significantly different among streams and time periods. Cumulative number of dry days, degree days, and redox depth all strongly correlated with assemblage structure as indicated by ordination scores. Our results suggest that subsurface invertebrates respond to leaves and roots as food sources, but assemblage composition is not substrate specific. Colonization of leaves and roots within stream beds by aquatic and terrestrial taxa supports the idea that headwater intermittent streams are important interfaces for the reciprocal exchange of energy and materials between terrestrial and aquatic ecosystems.     

Do hydraulic units define macroinvertebrate assemblages in mountain streams of central Argentina?

Methods to assess the physical habitat provide important tools for many aspects of river management. Hydraulic units (defined as a homogeneous patch of flow type and substrate) were described in mountain streams of Central Argentina and the distribution of macrozoobenthos in these habitat units was analyzed. Four streams from the upper Carcarañá River Basin (Córdoba, Argentina) were sampled in two hydrological periods. Hydraulic units (as substrate and flow type), current velocity, depth, macrophytes and macroalgae were assessed. Three benthic samples were taken in each hydraulic unit. A total of 12 hydraulic units were registered, which varied seasonally in their proportional abundance. The highest values of taxonomic richness, total abundance, diversity and evenness were found in the low-water period. The most heterogeneous hydraulic units (characterized by substrate of diverse grain size) presented the highest richness, diversity and evenness, whereas the highest total abundance was observed in hydraulic units with homogeneous substrate, such as bedrock or gravel sand. Canonical correspondence analysis grouped samples and taxa mainly in relation to the hydraulic units, and temporal variation in macroinvertebrate assemblages was observed. We found that the interaction between hydrological and geomorphological conditions affected benthic assemblages and that their organization is important at a mesoscale. Therefore, hydraulic units may be considered important tools in assessing stream integrity in lotic systems of central Argentina.     

Greener rivers in a changing climate?—Effects of climate and hydrological regime on benthic algal assemblages in pristine streams

Excessive biomass development of benthic algae is often considered undesirable, but understanding the causes is confounded by complex interactions among driving factors. Pristine rivers allow a benchmark where human interference should be limited to climate change. In this study a time series comprising >20 years of annual benthic algae surveys from two pristine, soft water, boreal stream sites is used to determine whether year-to-year variations in benthic algal assemblages and cover were related to climate (temperature, precipitation, North Atlantic Oscillation) or hydrological regime. Total benthic algal cover ranged from 6 to 100% at Atna (the outflow of the Atna River from Lake Atnasjø), and from 3 to 50% at the headwater stream Li. Climate and hydrological regime explained 18 - 74% of the variability in benthic algal assemblages and cover. Generally, more variance was explained at Li than at Atna, possibly because (i) aquatic bryophytes blurred nutrient-mediated effects of climate and hydrology at Atna, and (ii) the upstream lake buffered hydrological variation. Temperature was more important for explaining benthic algal assemblages and cover at Atna, while hydrology was more important at Li. Climate and hydrological regime had no major impact on benthic algal taxon richness. High temperatures were associated with high benthic algal cover, particularly at Atna, while high suspended particle concentrations were associated with reduced benthic algal cover at Li, possibly due to scouring. Cover of the cyanobacterium Phormidium sp. increased at Li with increasing temperature, and decreased with prolonged periods of high discharge. Current predictions of climate change would lead to a “greener” Atna (increased cover of benthic algae), while Li would become more “bluegreen” (more Phormidium sp. but less filamentous green algae). It would also lead to a slightly more “eutrophic” algal assemblage at Atna (as indicated by the PIT-index for ecological status assessment), while a possible drift of the PIT-index is less clear at Li. The differences between Atna and Li likely reflect differences among river types, and it seems possible to make some generalizations: climate will likely affect benthic algae in lake outlets primarily via temperature, while headwater streams will primarily be affected via altered hydrology and particle concentrations.     

Land-use influence on the functional organization of Afrotropical macroinvertebrate assemblages

Studies investigating the effects of human activities on the functional organization of macroinvertebrate communities in tropical streams and rivers are very limited, despite these areas witnessing the greatest loss of natural forests globally. We investigated changes in taxon richness, numerical abundance and biomass of macroinvertebrate functional feeding groups (FFGs) in streams draining different land-use types in the Sosiani-Kipkaren River in western Kenya. Twenty-one sites in river reaches categorized as forested, mixed, urban or agricultural were sampled during the dry and wet seasons. Collected macroinvertebrates were identified to the lowest taxon possible (mainly genus) and classified into five major FFGs; collector-gatherers, collector-filterers, scrapers, predators and shredders. There were significant (p < 0.05) spatial variation in habitat quality, organic matter standing stocks, total suspended solids, electrical conductivity, dissolved oxygen, temperature and nutrient concentrations across land-uses, with forested sites recording lowest values in mean water temperature, electrical conductivity and nutrients while recording highest levels in dissolved oxygen concentrations. Responses in macroinvertebrates to changes in land-use varied with richness, abundance and biomass showing differences within FFGs. Biomass-based metrics responded more strongly to change in land-use while taxon richness was the least predictive, indicating replacement of taxa within FFGs across land-use types. Higher shredder abundance, biomass and richness were recorded in forested streams which were cooler with protected riparian areas and high biomass of coarse particulate organic matter. Collector-gatherers dominated agricultural and urban streams owing to an abundance of particulate organic matter and nutrients, while scrapers responded positively to increased nutrient levels and open canopy in mixed and agricultural streams where primary production and algal biomass was likely increased. Overall, this study provides further evidence of the effects of agricultural and urban land-uses on tropical streams and rivers and contributes to the use of macroinvertebrate FFGs as indicators of ecological health.     

Active reintroduction of benthic invertebrates to increase stream biodiversity

A large number of restoration projects aims to improve the ecological quality of streams and rivers by focusing on the stream structure. However, improved habitat heterogeneity often does not lead to natural recolonization by sensitive freshwater macroinvertebrate communities, particularly when the recolonization potential is low and source populations are absent. In preliminary studies we tested whether natural substrate exposures could be used to sample and transport benthic macroinvertebrates. In this pilot study we used these previously tested natural substrate exposures to sample freshwater invertebrates in a donor stream in order to actively (re-)colonize a recipient stream. In the course of three reintroduction campaigns, we were able to accumulate over 350,000 benthic invertebrates, including 25 indicator taxa of the orders Ephemeroptera, Plecoptera and Trichoptera and 30 taxa scoring positive in the German Fauna Index. In total, 45 taxa, which did not occur in the recipient stream before, were reintroduced. They were transported gently within natural substrate exposures and released on a stream bottom area of 500 m² in the recipient stream. We intended to study if an increase of benthos fauna in a recipient stream is possible, and if this increase will eventually improve the ecological status. So far, the natural substrate exposure-method demonstrated to be an adequate tool to accumulate and transport benthic macroinvertebrates and, in general, has the potential to increase the biodiversity of streams when used as assisted migration measure.     

Effects of environmental factors and predation on benthic communities in headwater streams

Structure and composition of benthic macroinvertebrate assemblages were investigated during three consecutive years in six headwater streams that exhibit a high variation in environmental conditions, habitat structure and predatory pressure. We examined whether the abundance of functional feeding groups could be best predicted by the abundance of predators and some habitat and chemical variables. Mean density and biomass of macroinvertebrate functional feeding groups varied significantly throughout the study area. Stepwise multiple regression analyses revealed that both density and biomass of functional feeding groups was influenced primarily by chemical features of water. Shredder biomass and scraper density were also influenced by habitat features, the abundance of scrapers increasing in deeper localities at lower altitudes and with abundant macrophytes. The abundance of predatory invertebrates was related to the density and biomass of benthic prey. An influence of fish predation on invertebrate communities was not observed in the study streams. The finding that benthic communities in undisturbed headwater streams are mainly affected by water chemistry variables irrespective of fish predation and habitat features clearly highlight the sensitivity of functional feeding groups to changes in chemical features and their role as indicators for bioassessment.     

Contrasting effects of cross-ecosystem subsidies and predation on benthic invertebrates in two Pacific coastal streams

Cross-ecosystem subsidies, such as terrestrial invertebrates and leaf litter falling into water as resources for aquatic communities, can vary across environmental gradients. We examined whether the effect of terrestrial subsidy inputs on benthic invertebrates was mediated by resident coastal cutthroat trout (Oncorhynchus clarki) in two representative streams. We experimentally manipulated the input rates (reduced, ambient) of terrestrial subsidies (terrestrial invertebrates and leaf litter) as well as the presence or absence of cutthroat trout in the two streams. The hypothesis that the reduction of terrestrial subsidies to the stream influences benthic invertebrate assemblages was supported by experimental results. The treatments of terrestrial subsidy reduction and cutthroat trout presence had a significant negative effect on benthic invertebrate community biomass and shredder biomass in East Creek with high natural terrestrial subsidy input and small amount of large wood in channel. In contrast, results from Spring Creek with low subsidy input and large amount of large wood in channel showed that only the terrestrial subsidy reduction significantly reduced the biomass of shredders. The effects of the terrestrial subsidy input and trout predation on benthic invertebrate communities varied between the two streams. Our results indicate that a subsidy effect on benthic communities can vary between nearby streams differing in canopy and habitats. This study, with the major finding of highly context-dependent effects of spatial subsidies, suggests that the interplay of resource subsidies and predators on invertebrate community assemblages can be site-specific and context-dependent on habitat features.     

Ecological assessment of highly heterogeneous systems: The importance of taxonomic sufficiency

We assess the effects of taxonomic resolution (genus-family levels) on the ecological assessment of 39 highly heterogeneous ponds located in north-western Spain.Non-metric multidimensional scaling (MDS) and one-way analysis of similarities (ANOSIM) were used to investigate the effects of taxonomic resolution on the macroinvertebrate assemblage structure. The Mann Whitney U-test and discrimination efficiency were used to assess the ability of nine diversity measures (total richness, rarefied richness samples of 25, 50 and 100 individuals, Margalef's index, Pielou's evenness, Shannon-Weaver's index, Simpson's index and percent dominant taxon) to discriminate between acceptable (best available and good conditions) and unacceptable (moderate, poor and very poor) conditions using three levels of taxonomic resolution: (i) family, (ii) family plus subfamilies of benthic non-biting midges and (iii) genus level.Based on non-metric MDS, the macroinvertebrate assemblages of ponds of acceptable (A) and unacceptable (N) conditions were statistically undistinguishable, both at genus and family levels. On the other hand, based on several community metrics (total richness, Margalef index, etc.) the two sets of samples were statistically different, although only when the genus or the subfamily level was used and after Bonferroni correction. These results suggest that the structure of macroinvertebrate assemblages by itself is more sensitive than the specific composition in distinguishing the fauna living in acceptable and unacceptable conditions. Moreover, dealing with families including many taxa generally showing different tolerance to disturbance may lead to misclassification of ponds. We agree, however, that the two approaches, i.e. assemblage composition and diversity measures, are conceptually different and hence they should be used in combination for a better understanding of the response of single metrics.     

Assessing streams in Germany with benthic invertebrates: selection of candidate metrics

The process of selecting invertebrate-based candidate metrics for the German stream assessment system is described. The aim was to identify metrics indicating degradation types other than organic pollution and acidification (“general degradation”). For 18 out of 24 German stream types a data base of roughly 2,000 benthic invertebrate samples was generated; for each sample 79 metrics were calculated. Data on land use in the catchment were compiled for all sampling sites, together with data on hydromorphology for many sampling sites. Hydromorphological and land use parameters, which describe a clear gradient in the data sets were identified by Non-metric Multidimensional Scaling (NMS). Correlation analyses between land use/hydromorphological parameters and metric results were calculated separately for the individual stream types. Among those metrics best indicating catchment- and hydromorphological degradation in the majority of stream types are: proportion of Ephemeroptera, Plecoptera and Trichoptera; proportion of Plecoptera (mainly suited for mountain streams); number of Plecoptera taxa; proportion of xenosaprobic taxa; proportion of epirhithral preferring taxa. Differences of metric correlations between stream types and between degradation types are discussed, leading to a list of candidate metrics for assessing German streams.     

Benthic foraminiferal assemblages from Moreton Bay, South-East Queensland, Australia: Applications in monitoring water and substrate quality in subtropical estuarine environments

We assess species composition, assemblage structure and distribution of the benthic foraminiferal assemblages from diverse substrates in Moreton Bay, South-East Queensland, Australia. Analysis of 47 surface sediment samples revealed 69 species, three distinct foraminiferal assemblages and six sub-assemblages. The assemblages from the western Bay are characterized by stress tolerant taxa and the lowest diversity, whereas the assemblages from the eastern Bay are characterized by symbiont-bearing taxa and high diversity. We found a correlation between foraminiferal assemblages and substrate conditions that was indicative of strong environmental gradients (substrate type, water quality and salinity), from an urban-impacted assemblage in the westernmost part of the Bay, to a hyposaline, estuarine-influenced assemblage in the western Bay to a nearly normal marine to hypersaline assemblage in the eastern Bay. The FORAM Index was consistent with the changes in water and sediment quality gradient, from the western shoreline to the eastern Bay. Thus the foraminiferal assemblages of Moreton Bay make excellent bio-indicators of environmental changes in a subtropical, estuarine setting in eastern Australia.     

Long-term changes in polychaete assemblages of Botany Bay (NSW, Australia) following a dredging event

The long-term effects of marine aggregate dredging on near-shore benthic assemblages are still largely unknown, despite a global increase in demand for, and extraction of, marine aggregates. This study assessed the state of recovery of polychaete assemblages in Botany Bay, temperate NSW, Australia, at sites dredged for aggregate material more than 10 years previously. Sedimentary and faunal samples were collected from impact sites in Botany Bay, and from reference sites in nearby Pittwater estuary. This study was based on, and included data from, a study conducted by the Australian Museum at the same sites in the 2 years following cessation of dredging. Abundance, species richness and evenness of polychaetes, as well as overall polychaete assemblage structure, were compared between localities over time.     

Seasonal variation in benthic macroinvertebrate assemblages and water quality in an Afrotropical river catchment,northeastern Tanzania

Population growth and economic development have resulted in increased water demands, threatening freshwater resources. In riverine ecosystems, continuous monitoring of the river quality is needed to follow up on their ecological condition in the light of water pollution and habitat degradation. However, in many parts of the world, such monitoring is lacking, and ecological indicators have not been defined. In this study, we assessed seasonal variation in benthic macroinvertebrate assemblages in a tropical river catchment in northeastern Tanzania, which currently experiencing an increase in agricultural activities. We examined the potential of in-stream environmental variables and land-use patterns to predict the river macroinvertebrate assemblages, and also identified indicator taxa linked to specific water quality conditions. Macroinvertebrate abundance, taxon richness and TARISS (Tanzania River Scoring System) score were higher in the dry season most likely due to higher surface runoff from agricultural land and poorer water quality in the wet season. In the wet season macro invertebrates seem to be limited by chlorophyll-a, oxygen and phosphorous while in the dry season, when water flow is lower, nitrogen and turbidity become important. Substrate composition was important in both seasons. Given the fact that different selective filters limit macroinvertebrate assemblages in both seasons, a complete picture of water quality can only be established by monitoring in both seasons. Riparian buffer zones may help to alleviate some of the observed negative effects of agricultural activities on the river system in the wet season while limiting irrigation return flows may increase water quality in the dry season.