Improved approaches to ecotoxicity testing of petroleum products

Crude oils produced in the North West shelf of Western Australia are highly volatile, a characteristic not shared by most of the Northern Hemisphere crude oils on which internationally accepted toxicity test protocols were developed. Because of this volatility and some other factors, the LC50 and EC50 values obtained from acute toxicity tests will be significantly affected by the changes of toxicant concentration in test solutions during the period of exposure. To address these issues all steps of a standard protocol for crude oil toxicity testing have been revised. A systematic study has been performed on factors which affect petroleum hydrocarbon solubilisation in aqueous systems during test solution preparations. The influence of mixing time, agitation energy and volume/interface ratio on a hydrocarbon concentration in a water-soluble fraction (WSF) was studied for heavy, medium and light crude oils. A study of the sensitivity of marine unicellular algae to WSF of crude oils was conducted with Isochrysis sp., Nannochloropsis-like sp. and Nitzchia closterium. Total concentrations of hydrocarbons dissolved in test solutions were estimated by UV-spectrometry and GC/FID chemical analyses. When the toxicant concentration decreased during the exposure period, the EC50 values derived from initial or final concentrations either underestimate or overestimate toxicity, respectively. Therefore, weighted average concentrations (WAC) calculated for the whole test period were recommended for expressing hydrocarbon concentrations in test solutions of crude oils. Toxicity indices calculated from WAC of total hydrocarbons for different crude oils can be compared regardless of the rates of hydrocarbon loss.     

Effects of Scotian shelf natural gas condensate on the mummichog

The 96h LC₅₀ of the water soluble fraction (WSF) of natural gas condensate from the Sable Is., N.S. area to Fundulus heteroclitus was between 4 and 5 μg · ml⁻¹ naphthalene equivalents (initial concentration). Behavioural responses in the fish to sub-lethal concentrations of WSF were similar to those recorded for crude and fuel oils. Induction of the hepatic mixed function oxidase enzyme enthoxyresorufin O-de-ethylase (EROD) in sexually immature fish occurred at WSF concentrations below the LC₅₀, but during the prespawning period no induction occurred. EROD activity in unexposed fish showed seasonal variation consistent with differences in its inducibility.     

Impaired osmoregulation in the shrimp Palaemon adspersus exposed to crude oil extract

Palaemon adspersus is a hyper- and hypo-osmoregulating shallow-water shrimp. For a maximum period of 34 days P. adspersus were exposed to 20, 70, 100 and 200 ppb WSF of North Sea crude oil. The osmotic concentration of the haemolymph was measured by freezing point depression once a week. The ability to maintain hyper-osmolality was significantly decreased (p<0.05) after one, two and three weeks exposure to 200, 70 and 100 ppb, respectively. No effect was observed in 20 ppb. After an accidental exposure of shrimps to 24 h acute oil pollution, they were placed in pure sea water. The ability to maintain hyper-osmolality was reduced one week after the accidental oil exposure, but was regained within three weeks.     

Vitellogenin and lipovitellin from the prawn Macrobrachium borellii as hydrocarbon pollution biomarker

During reproduction vitellogenin (VTG) is transported to vitellogenic oocytes as a precursor of egg yolk lipovitellin (LV). As VTG synthesis is affected by environmental stressors, it is widely used as biomarker in endocrine disruption studies. However, it has seldom been employed to evaluate invertebrate hydrocarbon pollution. An ELISA with anti-LV antibody was developed to evaluate the impact of water-soluble fraction of crude oil (WSF) on Macrobrachium borellii vitellogenesis. Prawn VTG concentration was within the range reported for other crustaceans; LV values were positively correlated with gonadosomatic index (GSI). Females at different vitellogenic stages were exposed to a sub-lethal concentration of WSF for 7 days. Exposed animals with GSI>7 increased their VTG and LV titer as compared to control organisms (190% and 140%, respectively). VTG levels in M. borellii were upregulated and highly sensitive to WSF exposure. This assay could be employed as a biomarker for freshwater hydrocarbon pollution.     

Elimination of effects of earthquake in monitoring crustal movement

Monitoring crustal movements is important in understanding the earth surface and in developing theories about plate tectonics. Plate tectonics describes earth crust which consists of a number of plates moving relative to one another. Global plate models suggest that plate movements are constant due to the fact that relative movements of plates were represented by averaged plate motion. However, if an earthquake occurs, the uniform movement of related plates does not follow the regular trend. In analysis by Kalman Filter, the effects of an earthquake occured within crustal movement monitoring period by geodetic method have been eliminated by a proposed approach, Fading Memory Filter. The proposed method was tested using a real/simulated data. The results of the test showed that the effect of a strong earthquake (M_w = 7.1) which had occurred in the Gulf of Eilat/Aqaba was eliminated. Consequently, the proposed method is capable of removing or eliminating a suddan effect in monitoring crustal movements in the analysis by using the Kalman Filter.     

Seismic capacity of retrofitted beam–column connections in high‐rise steel frames when subjected to long‐period ground motions

The seismic capacity of beam‐to‐column connections in steel high‐rise frames is a matter of concern, particularly when they are subjected to long‐period ground motions. A previous full‐scale shaking table test conducted at the E‐Defense National Research Institute for Earth Science and Disaster Prevention in Japan disclosed cracks and fractures in such beam‐to‐column connections. This paper examines the effects of three types of beam‐to‐column connection retrofit: supplemental welds, wing plates, and a haunch. Quasi‐static member tests and a series of shaking table tests applied to a full‐scale specimen are conducted to quantify the respective performances of the retrofit schemes. The performance of a total of 28 connections tested by the member and shaking table tests is evaluated together with that of an additional 12 unretrofitted connections tested in the previous test. When the supplemental welds are applied only to the shear tab to the web, the connection fractures at the same instant as the connection without retrofit. The corresponding cumulative plastic rotation is not improved. When the supplement welds are further applied to the web‐to‐column connection, strain concentration at the bottom flange, primarily promoted by the presence of the RC floor slab, is significantly reduced, and the cumulative plastic rotation capacity is increased to eight times that of the connection without retrofit. For the wing plate connection and haunch connection, the critical section is moved from the beam end to the beam cross‐section corresponding to the tip of the wing plates or haunch, resulting in an improvement of ductility by eight times that of the unretrofitted connection. Copyright © 2011 John Wiley & Sons, Ltd.     

The Impact of the Herbicide Diuron on Photosynthesis in Three Species of Tropical Seagrass

The impact and recovery from exposure to the herbicide diuron [DCMU; 3-(3′,4′-dichlorophenyl)-1,1-dimethylurea] was assessed for three tropical seagrasses, maintained in outdoor aquaria over a 10-day period. Photosynthetic stress was detected using chlorophyll a fluorescence, measured with a Diving-PAM (pulse amplitude modulated fluorometer). Exposure to 10 and 100 μg l⁻¹ diuron resulted in a decline in effective quantum yield (ΔF/F_m^′) within 2 h of herbicide exposure in Cymodocea serrulata, Halophila ovalis and Zostera capricorni. Effective quantum yield also declined over the first 24 h of exposure in H. ovalis at even lower diuron concentrations (0.1 and 1.0 μg l⁻¹). Effective quantum yield in H. ovalis and Z. capricorni was significantly depressed at all diuron concentrations (0.1–100 μg l⁻¹) after 5 days exposure, whereas effective quantum yield in C. serrulata was only significantly lower in plants exposed to highest diuron concentrations (10 and 100 μg l⁻¹). Effective quantum yield depression was present 5 days after plants exposed to 10 and 100 μg l⁻¹ diuron were returned to fresh seawater. These results indicate that exposure to herbicide concentrations present in nearshore Queensland sediments present a potential risk to seagrass functioning.     

Eccentric installation of splice plates for compressive strength improvement of rectangular hollow section braces

Steel hollow section members are often applied as bracing in steel structures. Field-bolted connections of the slotted-in single splice plate and the gusset plate are popular because of their ease of construction. However, eccentricity between the splice and gusset plate axes reduces the compressive strength of the brace. This study proposes compressive strength improvement of rectangular hollow section braces using eccentrically installed splice plates such that the gusset plate axis coincides with the brace axis. To demonstrate the efficacy of the proposed concept, four compressive loading test results are examined in this study. Test results reveal the influences of splice plate eccentric installation on the brace compressive strength, the out-of-plane displacement, and the strain distribution. The proposed concept is effective for the brace with stiffened splice plates for inducing overall buckling with plastic hinges in the gusset plates. Variation of compressive strength is finally discussed based on the discrepancy between the brace axis and the axis of the plates in which the plastic hinges form at the overall buckling mode ends.     

Test and evaluation of modified TADAS devices with different grades of steel

Triangular-plate added damping and stiffness(TADAS) devices are reliable metallic energy dissipaters for seismic upgrading used in design and retrofitting of civil structures. Conventional TADAS devices are designed with closedended slots. In this study, a modified form of the TADAS device is proposed with open-ended slots in order to reduce the manufacture cost, facilitate the assembling and avoid abrupt stiffness increase. Cyclic and monotonic loading tests are then conducted to investigate the mechanical characteristics of the modified TADAS devices with regular Q345 steel and lowyield point LY160 steel triangular plates. The test results show that although the hysteresis performances are stable, the cyclic hardening behavior is different between the TADAS specimens with different grades of steel. The TADAS specimen with LY160 triangular plates exhibits more significant overstrength behavior than the one with Q345 triangular plates in cyclic loading, which is unsuitable to be described by the classic Bouc-Wen model. Therefore, a modified Bouc-Wen model is proposed to describe such overstrength behavior. It is shown that the modified model is able to simulate different extent of overstrength behavior in cyclic loading, based on which the cyclic hardening behavior of the TADAS specimen with LY160 triangular plates can be well described.     

Comparative evaluation of statistical tests for time series analysis: application to hydrological time series / Evaluation comparative de tests statistiques pour l'analyse de séries temporelles: application à des séries temporelles hydrologiques

Abstract

Statistical analyses of hydrological time series play a vital role in water resources studies. Twenty-nine statistical tests for detecting time series characteristics were evaluated by applying them to analyse 46 years of annual rainfall, 47 years of 1-day maximum rainfall and consecutive 2-, 3-, 4-, 5- and 6-day maximum rainfalls at Kharagpur, West Bengal, India. The performance of all the tests was evaluated. No severe outliers were found, and both the annual and maximum rainfall series were found to be normally distributed. Based on the known physical parameters affecting the homogeneity, the cumulative deviations and the Bayesian tests were found to be superior to the classical von Neumann test. Similarly, the Tukey test proved excellent among all the multiple comparison tests. These tests indicated that all the seven rainfall series are homogeneous. Two parametric t tests and the non-parametric Mann-Whitney test indicated stationarity in all the rainfall series. Of 12 trend detection tests, nine tests indicated no trends in the rainfall series. The Kendall's Rank Correlation test and the Mann-Kendall test were found equally powerful. Moreover, the Fourier series analysis revealed no apparent periodicities in all the seven rainfall series. The annual rainfall series was found persistent with a time lag of nine years. All the rainfall series were subjected to stochastic analysis by fitting 35 autoregressive moving-average (ARMA) models of different orders. The best-fit models for the original annual rainfall and 1-, 2- and 3-day maximum rainfall series were found to be ARMA(0,4), ARMA(0,2), ARMA(0,2) and ARMA(3,0), respectively. The best-fit model for the logarithmically transformed 4-day maximum rainfall was found to be ARMA(0,2). However, for the inversely transformed 4-, 5- and 6-day maximum rainfall series, ARMA(0,1) was obtained as the best-fit model. It is concluded that proper selection of time series tests and use of several tests is indispensable for making useful and reliable decisions.     

Oxygen consumption rate of shrimp exposed to crude oil extract

The shallow water shrimp Palaemon adspersus was exposed to 200, 100 and 0 ppb WSF of crude oil for 14 days after which the oxygen consumption rate was measured in a closed chamber with an electrode. Previous work has shown a changed oxygen consumption rate in animals exposed to WSF in concentrations 100 to 1000 times the concentrations used in the present experiments. The oxygen consumption rate was also significantly increased by the low concentrations used in the present work.Although the oxygen consumption rate was significantly increased, as an effect of WSF-pollution, the method is very time-consuming.     

Hydrological extreme variability in the headwater of Tarim River: links with atmospheric teleconnection and regional climate

Variability and possible relationship between monthly 1-day maximum/minimum flow from headwater of Tarim River basin, climatic indices and regional climate were detected by Mann–Kendall test, continuous wavelet transform, cross-wavelet and wavelet coherence methods. The results showed that: (1) hydrological extremes have increased during past 50 years, and the trends of 1-day minimum flow were larger than that of 1-day maximum flow. The most significant change occurred in winter; (2) the hydrological extremes exhibited significant 1-year period and 0.5-year period along the whole hydrological series; (3) different circulation indices may influence the trends of hydrological extremes in different river. The area of polar vortex in North American (i25) and area of Northern Hemisphere polar vortex (i5) showed most significant correlation with 1-day maximum flow and 1-day minimum flow in Aksu River, respectively. In Hotan River, the most significant correlated climate indices with 1-day maximum and minimum flow were Southern oscillation index and area of Northern American Subtropical High (i15), respectively. The area of polar vortex in Atlantic and Europe Sector (i35) showed significant relationships with 1-day minimum flow in Yarkand River; (4) regions of shared power at 0.8–1.5 year mode were found between selected climate indices and the hydrological extremes, anti-phase relations were detected for most of the series; (5) the fluctuations of temperature have strong effects on hydrological extremes, and significant coherence between regional climate and extremes was found at 0.7–1.5 year scale. The results of the study provide valuable information for improving the long-term forecasting of the hydrological extremes using its relationship with climate indices.     

Influence of calibration methodology on ground water flow predictions

We constructed a numerical model of transient ground water flow and solute transport for a portion of the Biscayne Aquifer in Florida, and calibrated the model with three different combinations of data from a 193-day period: head (h) data alone, data on h and ground water discharge to a canal (q), and data on h, q, and ground water chloride concentration (C). We used each of the three calibrated models to predict h and q during a 182-day test period separate from the calibration period. All three calibrated models predicted h equally well during the test period (r = 0.95, where r = 1 indicates perfect agreement between measured and simulated values), though the model calibrated on h alone had significantly different parameter values than the other two models. Predictions of q during the test period depended on calibration methodology; models calibrated with multiple targets simulated q more accurately than the model calibrated on h alone (r = 0.79 compared to r = 0.49). Based on the results of these simulations, we conclude: (1) Post-calibration prediction is important in assessing the value of different data types in automated calibration; (2) inverse-solution uniqueness is not a requirement for accurate h predictions; (3) relatively simple models can predict with reasonable accuracy transient ground water flow in a complex aquifer, and parameters governing this prediction can be estimated by nonlinear regression methods that incorporate both h and q data; (4) addition of C data to the calibration did not improve model predictive capacity because the information in the C data was similar to that in the q data, from the perspective of model calibration (the subsurface chemical signal in question was controlled mainly by seepage of high-chloride canal water into the low-chloride ground water system).     

Biokinetics of Radiocobalt in the Asteroid Asterias rubens (Echinodermata): Sea Water and Food Exposures

Uptake and loss of cobalt-57 were investigated in the starfish Asterias rubens, in order to assess its value as a sentinel organism for nearshore radionuclide contamination. Whole-body uptake from sea water was linear over a 32-day exposure period and reached wet weight concentration factor (CF) of 23 ± 5. Bioaccumulation of ⁵⁷Co was dependent upon body compartment, the aboral part of the body wall concentrating cobalt to the greatest degree (wet weight CF: 77 ± 16). After restoration of uncontaminated conditions, radiocobalt was released following an exponential loss kinetics characterized by a biological half-life (T_b1/2) of 27 ± 6 day. Dietary radiocobalt (taken up during a short-term feeding for 24 h on radiolabelled mussels) showed a much more rapid turnover time (T_b1/2: 14 ± 4 d), suggesting that A. rubens accumulates this radionuclide predominantly from sea water. A. rubens, and more particularly the aboral part of its body wall, would readily reveal the presence of an environmental contamination by radiocobalt and could preserve this information over a period of few months.     

Determination of column size for displacement‐based design of reinforced concrete frame buildings

This article reports a method to determine the storey‐wise column size for displacement‐based design of reinforced concrete frame buildings with a wide range of storey drift and building plan. The method uses a computer program based algorithm. The basic relation used in the algorithm is formulated by considering the various possible deformation components involved in the overall frame deformation. As a necessity to represent the deformation component due to plastic rotation of beam members, a relation between the beam plastic rotation and the target‐drift is adopted. To control the dynamic amplification of interstorey drift, a target‐drift dependant design‐drift reduction factor is used. The dynamic amplification of column moment is accounted with the help of an approximate conversion of fundamental period of the building from the effective period of the equivalent SDOF system. To avoid the formation of plastic hinge in column members, a design‐drift dependant column–beam moment capacity ratio is used. The method successfully determines the storey‐wise column size for buildings of four plans of different varieties, heights up to 12 storeys and target‐drift up to 3%. Copyright © 2013 John Wiley & Sons, Ltd.     

Relative importance of dissolved and food pathways for lead contamination in shrimp

The relative importance of dissolved and food pathways and the influence of food type in the bioaccumulation and retention of lead in the shrimp Palaemonetes varians were examined using a radiotracer method. Shrimp were exposed to ²¹⁰Pb-labelled seawater or fed two types of ²¹⁰Pb-labelled food, viz. mussels or worms. The amount of radiotracer accumulated by shrimp was examined over a 7-day period, followed by a 1-month and a 7-day depuration period for the dissolved and food source, respectively. Steady state in the uptake was reached after 2 days exposure to dissolved lead, with a resultant estimated concentration factor of 98 ± 3. Transfer factors following ingestion of contaminated mussels and worms were lower than unity for both food types, with lead transfer from worms being significantly higher than that from mussels. Accumulation of dissolved Pb by shrimp was found to occur mainly through adsorption on the exoskeleton with a minor accumulation in the internal tissues probably resulting from the intake of seawater for osmoregulation. In contrast, lead taken up from contaminated food was readily absorbed and bound in the internal tissues of P. varians. Although the transfer of lead to P. varians through the ingestion of contaminated food was low (TF < 1%), it still represented 4 to 8% of the lead content in the prey which is a significant additional contribution of lead to the shrimp body burden. Independent of food type, following ingestion of contaminated food, approximately 23–27% of total lead accumulated in shrimp was located in the edible parts (e.g. muscle). Therefore, the food pathway is suggested to be a significant contributor to the lead transfer to humans through ingestion of contaminated shrimp. After exposure to contaminated food, lead loss kinetics were described by a two-component model, whereas Pb loss following direct uptake from seawater was best described by a three-component model. The additional compartment representing 64% of total Pb retained and characterized by a turnover < 10 min, corresponded to lead weakly adsorbed on the exoskeleton and incorporated in the hepatopancreas. Nevertheless, a significant fraction of lead accumulated from the dissolved (2%) and food (52–57%) pathways remained irreversibly retained in the tissues, suggesting that this organism could also serve as an effective long-term bioindicator of lead contamination in marine waters.     

Simultaneous observations of the 2-day wave at London (43°N, 81°W) and Saskatoon (52°N, 107°W) near 91 km altitude during the two years of 1993 and 1994

Simultaneous observations are valuable in providing further insights into the character of the quasi 2-day wave. In this study we investigate the period and amplitude for the quasi 2-day wave near 91 km using MF radars at London and Saskatoon, Canada, and in addition look at possible values of the zonal wave number. The results of the present study bring out certain new aspects of the quasi 2-day wave at mid-latitudes in the Northern Hemisphere. In particular we show that the period of the quasi 2-day wave determined from our study (specially at large amplitudes) is smaller (46-47 h) than the 51-52 h period often suggested by other Northern Hemisphere results, and that the periods also showed variability as a function of time. We also draw attention to the annual variability, and especially highlight the occurrence of the wave in non-summer months. Our observations show significant wave correlation between the London and Saskatoon sites during time intervals of strong 2-day wave activity. These results suggest that the 2-day waves of 1993/4 are westward propagating waves of zonal wave number 3, although sometimes the zonal wave number 5 is also indicated (specially at large amplitudes). Our study also contributes additional mid-latitude geographical data which should aid in developing a better picture of the quasi 2-day wave.     

周期性层状介质中超声波传播的实验研究

实验研究了韵律层结构和超声波探测频率对弹性波波速和波形的影响。实验中所使用的超声波频率为 2 4 0kHz ,韵律层由不同厚度的钢板和有机玻璃板构成。研究表明 ,当λi/di<4 (i=1 ,2 ) (λi 为超声波在介质i中传播时的波长 ,di 为韵律层中介质i的厚度 )时 ,韵律层的波速近似等于 2种介质的时间平均速度 ;当λi/di>5 (i=1 ,2 )时 ,其速度开始显著地降低 ,并趋向于有效介质波速的Reuss下限 (VRp)。韵律层的结构对于透射波的波形有显著的影响。特别是当λi/di 接近于 6时 ,透射波波形非常简单 ,并且其透射波的振幅显著地降低。因此波形简单并不一定对应着简单的介质结构。由于弹性波在韵律层中的传播速度以及波形与探测频率有关 ,因此在利用地震测深结果分析岩石圈结构 ,推断其物质组成时应该充分考虑地震测深频率和地壳结构对分析结果的影响     

A Comparison of Growth Biomarkers for Assessing Sublethal Effects of Cadmium on a Marine Gastropod, Nassarius festivus

The effect of cadmium on the growth of an intertidal marine gastropod, Nassarius festivus, was investigated using a number of biomarkers namely conventional measurements in terms of increase in shell length or body weight, scope for growth (SfG) and RNA/DNA ratio. After eight days of cadmium exposure, the no observable effect concentration (NOEC) and lowest observable effect concentration (LOEC) for the rate of increase in wet weight were 1.00 and 1.38 mg/l, respectively, while those for shell length did not show any significant difference from the control until day 16. After 16 days of cadmium exposure, the LOEC for both shell length and wet weight measurements was 0.22 mg/l. The 8-day SfG test was sensitive to cadmium stress, showing a significant decrease at 0.16 mg/l cadmium when compared to the control. The NOEC and LOEC of RNA/DNA ratio determination were 0.52 and 0.93 mg/l, respectively. An assessment based on sensitivity and technical complexity suggests that Scope for Growth is the most sensitive growth biomarker, followed by the RNA/DNA ratio, and then the conventional growth measurement based on shell size and body weight.     

Growth of an amphipod and a bivalve in uncontaminated sediments: Implications for chronic toxicity assessments

This study assessed the sensitivity of the amphipod Chaetocorophium cf. lucasi, an inhabitant of New Zealand estuarine muddy sediments, and of the bivalve Macomona liliana, commonly found in New Zealand sandflats, to sediment particle-size distribution, total organic carbon, total organic nitrogen and water content. Growth and survival were evaluated after different test exposure times using laboratory-prepared natural sediment mixtures of sand and mud. The results suggest that amphipod survival was affected by the type of sand more than by the concentration of sand in the sediment samples. In samples with high survival rates, amphipod growth was impaired in sediments with higher sand proportions after a 10-day exposure, but after longer periods (28 or 30 days) there were no significant differences among treatments which had ≤76% sand. Bivalve survival and growth were not significantly affected by different natural sediment characteristics. Test duration for chronic toxicity tests with the bivalve M. liliana and the amphipod C. cf. lucasi should be at least 28 days for the detection of significant growth relative to the initial size.