Early development of Silvetia babingtonii (Fucales,Phaeophyceae)

Silvetia babingtonii is a potentially economic brown alga for sources of food and high-value added utilization. So far, sporeling nursery and field cultivation has not been successful. The lack of knowledge on development and life cycle of this alga hinder the development of techniques for the sporeings and cultivation. In this study, internal structure of oogonium and antherium of S. babingtonii was observed with hematoxylin and eosin staining and through microscope. Meanwhile, early development from zygotes to juvenile sporelings was studied at 20℃ under 60–100 μmol photons m-2 s-1. Zygotes germinated and divided into thallus and rhizoid cells. The larger thallus cells further divided and developed into juvenile sporelings; while the smaller rhizoid cells divided and elongated into rhizoid hairs. These findings documented the life cycle of S. babingtonii and provided fundamental knowledge for sporeling nursery in the near future.     

海南省2个冬青叶马尾藻种群的多样性及分子系统学研究

为了解海南省冬青叶马尾藻不同地理种群的多样性,从潭门镇和长坡镇收集到12份冬青叶马尾藻样品,通过PCR扩增和测序获得了其cox1(细胞色素C氧化酶亚基1)、rbc L(1,5-二磷酸核酮糖羧化酶/加氧酶大亚基)及ITS(内转录间隔区)序列。多序列比对显示,2个种群的冬青叶马尾藻的cox1、rbc L序列很保守,未发现多态性位点;而ITS序列变异较大,在碱基组成和序列长度上均有丰富的多态性。根据ITS的序列特征将所分析样品分为A、B和C 3个类群。系统进化分析显示,3个类群能以较高的置信度与数据库中其他地域来源的冬青叶马尾藻聚为一支,其中A、B类群的亲缘关系非常近,而C类群与其他地域来源的冬青叶马尾藻更相似。     

First Record and Redefinition of the Qingdao Population of Marine Ciliate Cardiostomatella vermiformis （Kahl, 1928） Corliss, 1960 （Protozoa, Ciliophora）

The living morphology and infraciliature of a marine ciliate, Cardiostomatella vermiformis isolated from a sand beach of the Jiaozhou Bay near Qingdao, China, were investigated by live observation, protargol and silver carbonate impregnation methods. Both the morphological and morphometric data largely agree with former records. Based on the data obtained, an improved diagnosis for Cardiostomatella vermiformis is supplied: Large marine Cardiostomatella with cylindrical body shape and several prolonged caudal cilia; cells in vivo about (90–500) μm×(30–120) μm; macronucleus beaded; single contractile vacuole caudally positioned; buccal apparatus conspicuously small, genus typical; three to six postoral kineties; 96-130 somatic kineties. This species is recorded for the first time in China.     

Evaluation and fusion of SST data from MTSAT and TMI in East China Sea, Yellow Sea and Bohai Sea in 2008

Two typical satellite sea surface temperature (SST) datasets, from the Multi-functional Transport Satellite (MTSAT) and Tropical Rainfall Measuring Mission Microwave Imager (TMI), were evaluated for the East China Sea, Yellow Sea, and Bohai Sea throughout 2008. Most monthly-mean availabilities of MTSAT are higher than those of TMI, whereas the seasonal variation of the latter is less than that of the former. The analysis on the one-year data shows that the annual mean availability of MTSAT (61%) is greater than that of TMI (56%). This is mainly because MTSAT is a geostationary satellite, which achieves longer observation than the sun-synchronous TMI. The daily availability of TMI (28%-75%) is more constant than that of MTSAT (9%-93%). The signal of infrared sensors on MTSAT is easily disturbed on cloudy days. In contrast, the TMI microwave sensor can obtain information through clouds. Based on in-situ SSTs, the SST accuracy of TMI is superior to that of MTSAT. In 2008, the root mean square (RMS) error of TMI and MTSAT were 0.77 K and 0.84 K, respectively. The annual mean biases were 0.14 K (TMI) and -0.31 K (MTSAT). To attain a high availability of SSTs, we propose a fusion method to merge both SSTs. The annual mean availability of fusion SSTs increases 17% compared to MTSAT. In addition, the availabilities of the fusion SSTs become more constant. The annual mean RMS and bias of fusion SSTs (0.78 K and -0.06 K, respectively) are better than those of MTSAT (0.84 K and -0.31 K).     

Life cycle and production of chironomidae (diptera) in Biandantang, a typical macrophytic lake (Hubei, China)

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Effect of salinity on growth and first sexual maturity of Exopalaemon carinicauda (Holthuis, 1950)

To determine the optimal salinity for growth and first sexual maturity of ExopaIaemon carinicauda, the effects of salinity on growth and reproductive performance of early juvenile prawns of E. carinicauda were evaluated under laboratory conditions. Postlarvae from the same female broodstock were reared at six salinity levels （5, 10, 15, 20, 25, and 30） for 12 weeks. The specific growth rate （SGR） and survival rate （SR） under different salinity levels in the first 6 weeks were calculated and compared. SGR was significantly influenced by salinity. Prawns reared in salinity of 10 grew significantly faster （P〈0.05） than those reared in salinities of 5, 20, 25, and 30. However, ANOVA confirmed that there was no significant effect among the six salinity levels on SR. For the next 6 weeks, the body length （BL）, body weight （BW） at the first sexual maturity, and the age at median sexual maturity （As0） of females were measured and compared. Female prawns reared at salinity of 10 presented significantly shorter A50, but no significant differences among the six salinity levels for BL and BW at the first sexual maturity were observed. Based on the above information, the optimal salinity for growth and first sexual maturity of juvenile E. carinicauda is approximately 10.     

Life history pattern of mosquitofish Gambusia holbrooki (Girard, 1859) in the Tajan River (Southern Caspian Sea to Iran)

Investigation of the ecology of introduced species in new habitats can allow determinations of the degree, direction, and rate of evolutionary change. The introduction of Gambusia holbrooki in the southern Caspian Sea presents such a situation. We evaluated the life history traits of mosquitofish in the Tajan River basin. A total of 744 G. holbrooki specimens were collected between January and December 2008. The maximum observed ages are 0+ years for males and 1+ years for females. Both sexes grew allometrically (negative for males: b=2.442 and positive for females: b=3.232). The overall sex ratio is unbalanced and dominated by females. GSI values suggest that this population of G. holbrooki matures between February–July. The highest mean GSI value is 1.80 for males and 15.97 for females in May. Egg diameter ranges from 1.00 to 3.00 mm with a mean value of 2.098 mm. Absolute fecundity varied from 7 to 57 eggs. Both ova diameter and absolute fecundity were positively correlated to fish size (length and weight). Fecundity relative to total weight fluctuated from 34.44 to 582.64 eggs/g, and to total length from 2.33 to 12.95 eggs/cm. Both were negatively correlated with female size. The characteristics of this population are important with respect to life history of the species.     

Ontogenetic development in the morphology and behavior of loach （Misgurnus anguillicaudatus） during early life stages

Loach （Misgurnus anguillicaudatus） is a commercially important fish in China and an ideal aquaculture species. However, culturists experience high larval and juvenile mortality during mass production. To provide insight into ways to improve larviculture techniques, we describe the morphological characteristics and behavior of loach during the larval and early juvenile stages. Yolksae larvae ranged from 2.8 to 4.0 mm body length （BL） between days 0 to 4; preflexion larvae ranged from 3.6 to 5.5 mm BL between days 4 to 6; flexion larvae ranged from 4.8 to 8.1 mm BL between days 5 and 14; and post-flexion larvae ranged from 7. l to 15.7 mm BL between days 11 to 27; the minimum length and age of juveniles was 14.1 mm BL and 23 d, respectively. Loach are demersal from hatch through to the early juvenile stages. A suite of morphological characteristics （e.g., external gill filament and ventral mouth opening） and behavioral traits have developed to adapt to demersal living. We observed positive allometric growth in eye diameter, head length, head height, and pectoral fin length during the early larval stages, reflecting the priorities in the development of the organs essential for survival. Our results provide a basis for developing techniques to improve the survival of larval and juvenile loach during mass production.     

特高压工程施工利用高分二号遥感影像进行水土保持远程监测研究

论述了遥感影像中图像正射校正、图像融合和支持向量机等遥感技术在国产高分二号影像中的应用,并展示了其研究成果应用于特高压工程水土保持远程监测效果。在实施中,对特高压工程施工期和试运行期分别进行监测,首先利用图像正射校正、影像融合以及支持向量机分类等遥感技术获取特高压工程水土保持远程监测各个要素面积,即施工期扰动土地总面积,试运行期扰动土地的整治面积、可恢复林草植被面积和林草类植被面积,进而计算水土保持效果评价的3个评价指标,即扰动土地整治率、林草植被恢复率和林草覆盖率。将研究成果应用于锡盟-山东特高压交流输变电施工过程中,实验结果表明,高分二号遥感影像技术能够有效应用于特高压工程水土保持远程监测,为特高压工程水保监督提供了优质低价、可长期持续的一种远程监测方法。     

Production dynamics and life cycle of dominant chironomids （Diptera, Chironomidae） in a subtropical stream in China： adaptation to variable flow conditions in summer and autumn

The production dynamics and trophic basis of 7 dominant species of chironomids were investigated in the area of a second-order river of the Hanjiang River basin, in central China from June 2003 to June 2004. The results showed that Tvetenia discoloripes was by far the most abundant chironomid, dominating the overall standing stock of the taxa. In terms of life cycle, Chaetocladius sp., Eukiefferiella potthasti and T. discoloripes developed 1 generation a year, whereas Microtendipes sp. and Pagastia sp. developed two, while Pentaneura sp. and Polypedilum sp. developed three. T. discoloripes was the most productive chironomid with 120.305 8 g/m2.a, Pentaneura sp. and E. potthasti had relatively high production values of >17 g/m2.a, and the rest were <10 g/m2.a. All the production temporal variation tended to follow biomass patterns. T. discoloripes, Chaetocladius sp. and Pagastia sp. concentrated most of their production in winter, whereas E. potthasti, Pentaneura sp. and Polypedilum sp. had relatively higher production throughout the year. Only Microtendipes sp. had a production that peaked in summer. The overlap in temporal distribution of production among the chironomid species was generally high (>0.5), especially for filter-collectors Microtendipes sp., Chaetocladius sp., Chaetocladius sp., T. discoloripes and Pagastia sp. All species except Pentaneura sp. consumed a large portion of amorphous detritus, constituting more than 90% of their diets, and contributing nearly 90% to their secondary production. All the 7 chironomids represent obvious adaptation to local highly variable climate in summer and autumn in life cycle pattern, production dynamics, and food type.     

ESTARFM模型在西藏色林错湖面积时空变化中的应用分析(1976-2014年)

湖泊（特别是内陆湖）作为全球气候变化的敏感区域,是气候变化与环境变异的指示器,其面积变化在一定程度上可反映区域的气候变化。因此,精确监测湖泊面积的时空变化,对分析区域生态环境变化具有重要的意义。本文基于ESTARFM时空数据融合模型,利用MODIS数据模拟了2000年后无法得到的Landsat数据;利用NDWI和MNDWI 2种水体指数并辅以DEM数据分析了1976-2014年西藏色林错湖湖面面积的时空变化;综合湖区周围6个气象站点的气象数据(1970-2014年),探究了湖面面积变化的原因及其对气候变化的响应。结果表明：(1)利用ESTARFM时空融合模型得到的Landsat-Like数据与真实的Landsat数据在水体信息提取方面具有较高的相关性,R²可达0.93,时空数据融合的结果可用于湖泊水体的信息提取;(2)近40年来（1976-2014年）,色林错湖处于持续扩张状态,面积呈较显著的增长趋势,增加了近711.652 km²,增幅为42.36%,年平均增长速率约为18.728 km²a^-1,增长最快时可达55.954 km²a^-1;湖面面积变化先后经历了平稳变化-迅速变化-平稳变化3个阶段;北部湖区在40年间变化最为明显,向北扩展了约22.812 km;2003-2005年,南部湖区已与雅根错湖连为一体,随后二者共同扩张;(3)气温的持续升高造成的冰雪融水补给增加可能是导致湖泊面积扩张的主要因素,风速的降低为次要因素,湖面的面积变化与降水量、日照时数的变化相关性不明显。     

Application of otolith shape analysis for stock discrimination and species identification of five goby species （Perciformes： Gobiidae） in the northern Chinese coastal waters

We tested the use of otolith shape analysis to discriminate between species and stocks of five goby species( Ctenotrypauchen chinensis, Odontamblyopus lacepedii, Amblychaeturichthys hexanema, Chaeturichthys stigmatias, and Acanthogobius hasta) found in northern Chinese coastal waters. The five species were well differentiated with high overall classification success using shape indices(83.7%), elliptic Fourier coefficients(98.6%), or the combination of both methods(94.9%). However, shape analysis alone was only moderately successful at discriminating among the four stocks(Liaodong Bay, LD; Bohai Bay, BH; Huanghe(Yellow) River estuary HRE, and Jiaozhou Bay, JZ stocks) of A. hasta(50%–54%) and C. stigmatias(65.7%–75.8%). For these two species, shape analysis was moderately successful at discriminating the HRE or JZ stocks from other stocks, but failed to effectively identify the LD and BH stocks. A large number of otoliths were misclassified between the HRE and JZ stocks, which are geographically well separated. The classification success for stock discrimination was higher using elliptic Fourier coefficients alone(70.2%) or in combination with shape indices(75.8%) than using only shape indices(65.7%) in C. stigmatias whereas there was little difference among the three methods for A. hasta. Our results supported the common belief that otolith shape analysis is generally more effective for interspecific identification than intraspecific discrimination. Moreover, compared with shape indices analysis, Fourier analysis improves classification success during inter- and intra-species discrimination by otolith shape analysis, although this did not necessarily always occur in all fish species.     

Biomass and carbon storage of Gracilariopsis lemaneiformis （Rhodophyta） in Zhanshan Bay,Qingdao, China

Marine macroalgae can absorb carbon and play an important role in carbon sequestration. As an important economic macroalga, Gracilariopsis lemaneiformis has the potential to significantly affect carbon absorption and storage in wave-sheltered intertidal reef systems. However, detailed knowledge on seasonal biomass changes and carbon storage of G. lemaneiformis is lacking, especially in many small and scattered ecosystems. Considering the influence of human activities on wild distribution of G. lemaneiformis, the understanding of seasonal dynamics of an economically important species in nature is necessary. In this study, we first investigated seasonal variations in biomass, coverage area, and carbon storage during low tide from August 2011 to July 2012 in Zhanshan Bay, Qingdao, China. Furthermore, we estimated the carbon storage potential of wild G. lemaneiformis using light use efficiency(LUE). The results show that the standing biomass and coverage area changed significantly with season. However, seasonal variations in carbon content and water content were not obvious, with an average content of 35.1% and 83.64%, respectively. Moreover, carbon storage in individual months varied between 0.67 and 47.03 g C/m 2, and the value of carbon storage was the highest in August and June and the lowest in February. In Zhanshan Bay, LUE of G. lemaneiformis was only 0.23%. If it is increased to the theoretical maximum(5%–6%), the carbon storage will have an increase of at least 21 times compared with the current, which suggested that carbon storage of wild G. lemaneiformis had a high enhancement potential. The study will help to assess a potential role of G. lemaneiformis in reducing atmospheric CO2.     

Inter-annual variation of streamflow,precipitation and evaporation in a small humid watershed (Chengcun Basin,China)

For sustainable water resource management,it is important to determine the relationship between streamfl ow and other variables that infl uence availability of water resources.However,many catchments do not have suffi cient data to allow for a more detailed study of these relationships.We faced a similar challenge in the Chengcun Basin(limited historical data: from 1986–1999); and therefore we used a new approach to overcome this.We found that,using nonparametric trend methods in conjunction with the climate elasticity analyses and the 2D visualization of hydrologic data,it is possible to assess the relationships between streamfl ow and other hydro-climatic variables.In the past,streamfl ow trends in the basin were more correlated with precipitation than with potential evapotranspiration(PET).In addition,there is a gradual shift in the hydrological regime of the catchment,which may affect the occurrence of available water resources and activities that depend on them.In addition,based on our climate sensitivity analyses,the streamfl ow is dependent and more sensitive to variations in precipitation than to PET(δQ=0.79δP+0.42δE; precipitation elasticity,ε P=1.32; PET elasticity,ε E=-2.10).Therefore pending more detailed studies,the use of our approach will provide a rapid means to assess the variation of water resources(streamfl ow)in a watershed.In the future,we hope to carry out related research in other watersheds and also perform a more detailed studies to improve upon the results of this study.     

东昆仑地区中生代熔融、同化、存储和均一(MASH)过程及壳幔岩浆混合

哈西亚图石英闪长岩是东昆仑地区中生代具幔源组分贡献的花岗岩类典型代表,岩体出露于东昆仑中构造带,广泛发育暗色微粒包体。包体为闪长质岩石,并含有一系列岩浆混合成因的证据,如水滴状、长条状塑性流变外形,淬冷边、反向脉等高—中温混合迹象,以及低Mg/(Fe+Mg)、Na/(Ca+Na)值等混合成因特征。包体A/CNK值介于0.77~0.87,属准铝质,富Al2O3、Fe2O3、MgO,贫K2O、Na2O,富集大离子亲石元素(Rb、Ba、K等),同时又具有Ta、Nb、Ti的"TNT"负异常,具有俯冲带幔源岩石的成分特点。依据岩石学、地球化学特征并结合同时期大地构造背景,东昆仑晚古生代—早中生代含暗色微粒包体花岗质岩石是幔源岩浆经历多次熔融、同化、存储和均一(MASH)过程后与壳源岩浆混合的产物。在混合岩浆中,富镁铁质端元是由辉长质岩浆进化而来的闪长质岩浆。     

Spatial and seasonal distribution of macroinvertebrates in high altitude reservoir (Beyler Reservoir, Turkey)

A highland reservoir in the West Black Sea region of Turkey which belongs to the Mediterranean climatic zone was examined.Both littoral and profundal zones were sampled from October 2009 to September 2010,to determine taxonomic composition,biodiversity and abundance of benthic invertebrates as well as the seasonal variation of these measures.A total of 35 taxa were identified,of which 12 belong to Chironomidae and 10 to Oligochaeta groups.The highest diversity and abundance of benthic macroinvertebrates were found at the littoral stations.Macroinvertebrates showed significant positive correlations with water temperature and NO₂ and NO₃ concentrations,and negative correlation with dissolved oxygen.     

Cloning,characterization,and expression analysis of the DEAD-box family genes,Fc-vasa and Fc-PL10a,in Chinese shrimp (Fenneropenaeus chinensis)

RNA helicases of the DEAD-box and related families are involved in various cellular processes including DNA replication,DNA repair,and RNA processing.However,the function of DEAD-box proteins in aquaculture species is poorly understood at molecular level.We obtained the full-length cDNA sequences of two genes encoding helicase-related proteins,Fc-vasa and Fc-PL10a,from the testes of Chinese shrimp,Fenneropenaeus chinensis.The two predicted amino acid sequences contain all the conserved motifs characterized ...