广东省应大力发展海洋牧场

综述了我国及广东省十多年来鱼虾种苗人工放流及人工鱼礁的投放情况，结合广东省广大海区的水温、盐度、ｐＨ值、海流、营养盐含量情况及天然饵料的种类、分布等，提出了除在大亚湾已设置了水产增殖站以外，还应该在广东东部的南澳和西部的湛江各设一个增殖站，会收到更大的效果。人工鱼礁应总结过去投放的经验，加强礁区管理，逐步解决投资与收益的关系问题，并进一步扩大投放人工鱼礁的地点和密度，把广东海区切实建成理想的海洋牧场。     

Application of hydroacoustics to investigate the distribution,diel movement,and abundance of fish on Zhubi Reef,Nansha Islands,South China Sea

A combination of traditional fish sampling methods(hand-line and gill net) and modern hydroacoustic techniques were used to study fish community structure,distribution,and diel movements of fish on Zhubi Reef to enhance understanding of the ecosystem.We collected 126 individuals from 29 species,20 genera,17 families,three orders,and two classes using traditional gear.Perciforms were the dominant group in terms of species richness and biomass.The acoustic data indicated that very small(target strength[TS],dB) -60 dB) and small(-60 dB≤TS-45 dB) fish contributed the most to abundance and species richness on the coral reef,and that the proportion of medium-sized(-45 dB≤TS-35 dB) and largesized(-35 dB≤TS) fish increased gradually as depth increased.The single-target detection method revealed two distinct size classes during the day in the 12-16 and 16-20-m layers.One group consisted of very smallsized fish(TS-60 dB) and the other consisted of medium and large-sized fish(TS-55 dB).The number of single-target detections was significantly higher during the night than during the day(P0.05).The singletarget TS frequency distribution during the day was significantly different than during the night at depths of4-8,8-12,12-16,and 16-20 m.Significant differences were observed among the 4-8,8-12,12-16,and16-20-m-depth layers during day and night.Diel vertical movement was evidenced as fish began to spread and move upward just before sunset and began to assemble and descend shortly(15 min) after sunrise.     

A Comparative Study of Fish Assemblages Near Aquaculture,Artificial and Natural Habitats

Habitat plays a critical role in regulating fish community structure. Using the data collected from a monthly trammel net survey in Ma’an archipelago off the east coast of China, we evaluated impacts of five different habitats(artificial reefs, mussel farms, cage aquaculture, rocky reefs and soft bottom) on fish assemblages. This study suggests that artificial reefs(AR) have significantly higher species richness, abundance and diversity than mussel farms(MF) or soft bottom(SB) habitats during most seasons, and that fish taxa in the AR habitats are similar to those in the rocky reef(RR) habitats. Two different fish assemblage patterns were revealed in the study area using non-metric multidimensional scaling ordination: an assemblage dominated by reef fishes(especially by Scorpaenidae species) in AR, RR and cage aquaculture(CA) habitats and an assemblage dominated by Sciaenidae species in MF and SB habitats. We suggest that reef fishes play a key role in differentiating fish community structures in the study area. Although few differences in fish abundance and diversity were found between the CA and SB habitats, a more diverse age structure was observed in the CA habitats. A much more complex fish assemblage and enhanced population of local species were established as a result of the presence of both floating and fixed artificial structures, probably through improved survival rates.     

Histopathological Observation of Lymphocystis Disease and Lymphocystis Disease Virus （LCDV） Detection in Cultured Diseased Sebastes schlegeli

Lymphocystis nodules occurring in the cultured sting fish Sebastes schlegeli were observed under light and electron microscope. Lymphocystis disease virus (LCDV) in the tissues of diseased fish was detected with indirect immunofluorescence test (IFAT). Results showed that lymphocystis cells had overly irregular nuclei, basophilic intracytoplasmic inclusion bodies with virions budding from the surface, and hyaline capsules outside the cell membrane. Numerous virus particles about 200 nm in diameter scat- tered in the cytoplasm, electron-dense particles 70-80 nm in diameter filled in perinuclear cisterna, and membrane-enveloped parti- cles with electron-dense core of 70-80 nm appeared around cellular nucleus. IFAT using monoclonal antibody against LCDV from Paralichthys olivaceus revealed that specific green fluorescence was present in the cytoplasm of lymphocystis cells, epithelium of stomach, gill lamellae, and muscular fibers under epidermis of S. schlegeli, just as that in the cytoplasm of lymphocystis cells of P. olivaceus, suggesting the presence of LCDV in these tissues.     

REDUCED GROWTH IN HYBRID TILAPIA (OREOCHROMIS MOSSAMBICUS×O. NILOTICUS) AT INTERMEDIATE STOCKING DENSITY

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Reduced growth in hybrid tilapia (Oreochromis mossambicus ×O. Niloticus) at intermediate stocking density

Hybrid tilapia were reared at densities of 1, 5 or 10 fish per tank for four weeks. Mortality was 0 at 1 and 10 fish per tank, but was 25% at 5 fish per tank. Specific growth rate was highest at 1 fish per tank, and lowest at 5 fish per tank. The lower growth rate at the intermediate stocking density was associated with reduced feed efficiency, but there was no reduction in feed intake or digestibility. The results suggested increased metabolic cost caused by aggressive behaviour at intermediate stocking density, which can be suppressed by a further increase in density. This project was financially supported by the State Key Laboratory of Freshwater Ecology and Biotechnology of China and by Shanghai Fisheries University (Project SFU. 200003).     

Preliminary study on mitochondrial 16S rRNA gene sequences and phylogeny of flatfishes （Pleuronectiformes）

A 605 bp section of mitochondrial 16S rRNA gene from Paralichthys olivaceus, Pseudorhombus cinnamomeus, Psetta maxima and Kareius bicoloratus, which represent 3 families of Order Pleuronectiformes was amplified by PCR and sequenced to show the molecular systematics of Pleuronectiformes for comparison with related gene sequences of other 6 flatfish downloaded from GenBank. Phylogenetic analysis based on genetic distance from related gene sequences of 10 flatfish showed that this method was ideal to explore the relationship between species, genera and families. Phylogenetic trees set-up is based on neighbor-joining, maximum parsimony and maximum likelihood methods that accords to the general rule of Pleuronectiformes evolution. But they also resulted in some confusion. Unlike data from morphological characters, P olivaceus clustered with K.bicoloratus, but P cinnamomeus did not cluster with t3. olivaceus, which is worth further studying.     

湛江港海域游泳动物群落结构及多样性分析

【目的】研究湛江港海域的游泳动物资源结构及多样性特征。【方法】根据2016—2017年湛江港海域4个季度的底拖网渔业资源调查数据,采用相对重要性指数(index of relative importance,IRI)、Margalef丰富度指数(D)、Shannon-Wiener多样性指数(H')、Pielou均匀度指数(J)和ABC曲线(abundance-biomass comparison curve)分析该海域游泳动物的种类组成、优势种和多样性水平等群落结构特征。【结果】该海域共发现游泳动物173种,隶属于16目68科116属;其中鱼类种类数最多(98种,占总种类数的56.7%),以底层鱼类和暖水性鱼类为主;其次是甲壳类(66种,占38.2%),头足类最少(9种,占5.2%)。4个季节的优势种累计有11种(鱼类6种、甲壳类5种),其中条纹叫姑鱼(Johnius fasciatus)是春、夏、秋3个季节的共同优势种。从时间维度上看,秋季的H'、D均为最高,而春季的J最高;从空间维度上看,湛江港口门处S6站位多样性水平相对较高。ABC曲线结果显示,湛江港海域游泳动物群落在冬季受干扰程度高于其它3个季节。【结论】湛江港海域游泳动物种类丰富,其中鱼类是主要的游泳动物类群。     

The application of stereo-video technology for the assessment on population change of black rockfish Sebastes schlegeli in a vessel reef area in Haizhou Bay,China

The assessment of population structure and abundance of fish assemblages associated with artificial reefs(ARs) is an important aspect of AR management.In the present study,we used a DiveOperated Stereo Video(stereo-DOV) technique to assess the population structure and abundance of Sebastes schlegeli associated with two metallic,and three wooden,vessel reefs in Haizhou Bay during 2012 and2013.The study used video systems to obtain length measurements and estimates of abundance.The size composition of S.schlegeli differed among reefs and individuals around vessel reefs were all adults,with total lengths(TL) of 20 cm.Juvenile fish were encountered by divers in a rocky area near the island away from the vessel reefs.The largest individual S.schlegeli(with the highest TL) among five reefs were found around a metallic vessel reef in both 2012 and 2013.TL of S.schlegeli from all reefs increased by an average of 3.2 cm(P0.05) from 2012 to 2013,with an estimated mean weight increase of 250.4 g(P0.05).The video survey also indicated a decrease in the biomass of schools near two metallic vessels between the years.Stereo-video technology was found to be suitable for rockfish surveys around the reefs.     

Spatiotemporal distribution of fish eggs and larvae in the Huanghe(Yellow) River estuary,China in 2005–2016

Decreasing fish resources in estuaries is a subject of anthropogenic activities.Studies of the spatiotemporal distribution of fish eggs and larvae can help identify the status and processes underlying recruitment in a fishery.As the fifth largest river estuary in the world,the Huanghe(Yellow)River estuary(HRE)is a typical estuary that has been seriously affected by human activities.Annual surveys on ichthyoplankton and environmental factors were conducted in the months of May of 2005 and 2009-2016 in the HRE to investigate the spatiotemporal distribution of fish eggs and larvae and the associated influencing factors.A total of 23 and 20 species of eggs and larvae,respectively,were collected.The dominant orders were Perciformes(51.2%)and Clupeiformes(25.6%).The average number of fish species eggs and larvae were 6.0 and 4.1 in average abundance of 0.91 and 0.13 ind./m~3,respectively.The dominant species were mainly low-commercial-value small-sized fishes,such as Clupanodon punctatus,Hare,ngula zutnasi,and Acanthogobius,whereas certain traditional commercial fishes,such as Trichiurus lepturus,and Clupea pallasii,were not seen.Analysis of the fish egg and larval community revealed four temporal assemblages and two spatial assemblages.Salinity was the main factor on the spatial distribution of ichthyoplankton communities,the species number and Shannon-Weiner diversity index(H')of the fish egg and larval community near the river mouth with lower salinity were significantly lower than the community far away from the river mouth with higher salinity.In addition,increases of water temperature promoted the number and abundance of fish species eggs,and the areas of abundant prey tended to have a more diversified and abundant of ichthyoplankton species.In overall,overfishing,dam construction,and other human activities were the main drivers that led to the substantial decline in fishery resources in the HRE.     

Edible Peanut Worm(Sipunculus nudus) in the Beibu Gulf: Resource,Aquaculture, Ecological Impact and Counterplan

Sipunculus nudus is an important economic species because of its high nutritional and medicinal values. The exploitation and utilization of S. nudus primarily occur in the coastal regions of the Beibu Gulf. However, wild resource of S. nudus is rapidly decreasing because of the overexploitation, which has led to considerable developments of artificial breeding techniques. The cultivation scale of S. nudus has increased in response to successful artificial breeding; however, methods for culturing S. nudus in tidal flats or ponds require further study. Most studies have focused on the breeding, nutrition, medical value and ecological impact of these worms. Studies on the distribution, sediment requirements, nutrition characteristics, breeding techniques and aquaculture ecology of this species are summarized in this paper to promote the development of the aquaculture industry for S. nudus. The high biomass of S. nudus in the Beibu Gulf is positively correlated with the sediment characteristics and water quality of the region. The production of peanut worm has improved to some extent through culturing; however, the nutrient value and ecological environment problems have been observed, which reflect the over exploitation of trace elements and the sediment. These problems will worsen unless they are resolved, and the release of organic materials, nitrogen and phosphorus during harvesting impacts the coastal environment. Moreover, genetic erosion is a potential risk for larvae in artificial breeding programs in tidal flats. Therefore, culturing and collecting methods should be improved and the wild resource conservation should be implemented to promote the sustainable development of the peanut worm.     

徐闻珊瑚礁礁栖无脊椎动物分布

采用均匀定点潜水观察和采集标本调查表明,徐闻珊瑚自然保护区除分布有40多种珊瑚 外,尚分布有隶属于软体动物门、节肢动物门、棘皮动物门、环节动物门、腔肠动物门和海绵动物门 的55科115种礁栖无脊椎动物,分布的方式主要有穴居、附着和固着等3种方式,部分种类在礁丛 中索饵而隐蔽或躲避在礁缝隙中。     

Composition and distribution of planktonic ciliates in the southern South China Sea during late summer: Comparison between surface and 75 m deep layer

Ciliates are very important components in most marine ecosystem.They are trophic link between the microbial food web and grazing food chain.In this study,ciliates were collected from 11 sites in the southern South China Sea(SCS) during August 25 to September 28,2011.Their composition and distribution at the surface and 75 m deep depth of the ocean were studied.A total of 30 species belonging to 22 genera were identified,and 22 species of 15 genera were Tintinnids.Eutintinnus fraknoii and E.stramentus were the most common species.The other dominants were strombidiids ciliates including Strombidium conicum and S.globosaneum,which were followed by the tide form,Mesodinium pulex.Ciliates abundance ranged from 46 ind L~(-1) to 368 ind L~(-1) in the open sites,46–368 ind L~(-1) at surface and 73–198 ind L~(-1) at 75 m deep layer.In the Yongshu reef,ciliates abundance ranged from 167 ind L~(-1) to 365 ind L~(-1) in the water column,similar to that in Sanya coral reef waters.Ciliates composition showed obvious difference between surface and 75 m deep layer at station S2(P 0.05),while no similar result was observed at other sites.At 75 m deep layer,salinity was negatively related to mixed layer depth(P 0.05),but positively to chlorophyll a concentration(P 0.05),indicating that the change of vertical mixing in water column influenced vertical distribution of ciliates in the southern SCS.     

Genetic variation of natural and cultured stocks of Paralichthys olivaceus by allozyme and RAPD

Population genetics of the left-eyed flounder, Paralichthys olivaceus, including natural and cultured stocks distributed in the coastal waters near Qingdao of eastern maritime China, was analyzed in allozyme and RAPD. The results showed that among total 29 gene loci of 15 isozymes, 9 and 7 were po- lymorphic in natural and cultured stocks, respectively. The status of genetic diversity in P. olivaceus is low in terms of polymorphic loci in chi-square test and genetic departure index of Hardy-Weinberg equi- librium. More alleles in IDHP, CAT, GDH and Ldh-C allozymes were found in the fish, which could be used as markers in assortive breeding and distinguishing stock, population or species evolution. Total 88 and 86 RAPD bands ranging from 200 to 2 500 bp were recognized individually in average of 7.8–8.0 bands per primer. The genetic diversity in cultured stock is lower than that in natural ones showing an ob- viously decreasing genetic divergence. Therefore, effective countermeasures must be taken to protect ge- netic resources of marine cultured fishes. The 2 markers have their own pros and cons. Combining the 2 markers to investigate the genetic variation of populations is suggested. The results provide basic data of this flounder and they are useful for studying genetic improvement and genetic resources of the fish.     

Hydroacoustic estimates of fish biomass and spatial distributions in shallow lakes

We conducted acoustical surveys with a horizontal beam transducer to detect fish and with a vertical beam transducer to detect depth and macrophytes in two typical shallow lakes along the middle and lower reaches of the Changjiang(Yangtze) River in November 2013. Both lakes are subject to active fish management with annual stocking and removal of large fish. The purpose of the study was to compare hydroacoustic horizontal beam estimates with fish landings. The preliminary results show that the fish distribution patterns dif fered in the two lakes and were af fected by water depth and macrophyte coverage. The hydroacoustically estimated fish biomass matched the commercial catch very well in Niushan Lake, but it was two times higher in Kuilei Lake. However, acoustic estimates included all fish, whereas the catch included only fish 45 cm(smaller ones were released). We were unable to determine the proper regression between acoustic target strength and fish length for the dominant fish species in the two lakes.     

Significant population genetic structure detected in the rock bream <Emphasis Type="Italic">Oplegnathus fasciatus</Emphasis> (Temminck &amp; Schlegel, 1844) inferred from fluorescent-AFLP analysis

Oplegnathus fasciatus (rock bream) is a commercial rocky reef fish species in East Asia that has been considered for aquaculture. We estimated the population genetic diversity and population structure of the species along the coastal waters of China using fluorescent-amplified fragment length polymorphisms technology. Using 53 individuals from three populations and four pairs of selective primers, we amplified 1 264 bands, 98.73% of which were polymorphic. The Zhoushan population showed the highest Nei’s genetic diversity and Shannon genetic diversity. The results of analysis of molecular variance (AMOVA) showed that 59.55% of genetic variation existed among populations and 40.45% occurred within populations, which indicated that a significant population genetic structure existed in the species. The pairwise fixation index F _st ranged from 0.20 to 0.63 and were significant after sequential Bonferroni correction. The topology of an unweighted pair group method with arithmetic mean tree showed two significant genealogical branches corresponding to the sampling locations of North and South China. The AMOVA and STRUCTURE analyses suggested that the O. fasciatus populations examined should comprise two stocks.     

Status and historical changes in the fish community in Erhai Lake

Erhai Lake is the second largest freshwater lake on the Yunnan Plateau, Southwest China. In recent decades, a number of exotic fish species have been introduced into the lake and the fish community has changed considerably. We evaluated the status of the fish community based on surveys with multimesh gillnet, trap net, and benthic fyke-net between May 2009 and April 2012. In addition, we evaluated the change in the community using historical data (1952–2010) describing the fish community and fishery harvest. The current fish community is dominated by small-sized fishes, including Pseudorasbora parva, Rhinogobius giurinus, Micropercops swinhonis, Hemiculter leucisculus, and Rhinogobius cliffordpopei. These accounted for 87.7% of the 22 546 total specimens collected. Omnivorous and carnivorous species dominated the community. A canonical correspondence analysis (CCA) plot revealed that the distribution of fishes in the lake is influenced by aquatic plants, water temperature, pH, and season. The abundance of indigenous species has declined sharply, and a majority of endemic species have been extirpated from the lake (a decrease from seven to two species). In contrast, the number of exotic species has increased since the 1960s to a total of 22 at present. The fishery harvest decreased initially following the 1960s, but has since increased due to the introduction of non-native fish and stocking of native fish. The fishery harvest was significantly correlated with total nitrogen, not total phosphorus, during the past 20 years. Based on our results, we discuss recommendations for the restoration and conservation of the fish resources in Erhai Lake.     

尖吻鲈苗种工厂化培育模式的初步研究

采用室内水泥池及活饵培育配套技术进行尖吻鲈苗种阶段工厂化育苗。投放刚孵化出膜的仔鱼１６０万尾,经室内池８ｄ的培育,成活率为９０％;经１３ｄ的培育其规格超过１ｃｍ,成活率为５６．９％;经２４ｄ的培育平均规格达２ｃｍ左右,平均成活率为５０％,共育出８０万尾。苗种生长快,体质健壮,成活率高。关键技术是：基础设施配套;采用不同活饵,做到适口、及时转化、足量;定期过筛分级培育;水质控制良好;注意防病。２ｃｍ以上鱼种阶段采用多种方式培育,经１５～２０ｄ的饲养,规格达３～４ｃｍ,平均成活率为６５％～７５％。     

繁茂膜海绵对工厂化养鱼水体微生物净化作用的初步研究

在大菱鲆(Scophthalmus maximus)工厂化养殖废水中接种繁茂膜海绵(Hymeniacidon perleve),并将繁茂膜海绵与大菱鲆同池养殖,研究繁茂膜海绵对大菱鲆工厂化养鱼水体微生物的净化作用。结果表明,接种量为7 mg/L繁茂膜海绵经过8、16、24、32、40、48 h对养鱼废水中细菌总数的清除率分别为23.18%、47.65%、66.09%、81.57%、93.80%、98.96%;对弧菌的清除率分别为48.61%、56.44%、78.13%、92.88%、97.10%、98.56%;对大肠杆菌的清除率分别为39.51%、41.90%、73.24%、92.29%、97.69%、99.14%,将繁茂膜海绵以7 mg/L的生物量与大菱鲆同池养殖24 h,水体中细菌总数、弧菌和大肠杆菌的清除率分别为61.38%、50.34%、93.47%。