Diverse methane concentrations in anoxic brines and underlying sediments,eastern Mediterranean Sea

Elevated methane (CH₄) concentrations (128–2692×10³ nM) occur in the hypersaline anoxic brine pools of Bannock and Urania Basins, eastern Mediterranean Sea, compared to low concentrations (17–80×10³ nM) in the sediments below the anoxic brines. The CH₄ enrichment in the brines might be due to the long residence time of the brine in the Basin. An attempt is made to determine the sources for the enriched dissolved CH₄ by considering the influence of hydrothermal activity, the occurrence of sapropel layers (biogenic) and dissolution of gas hydrates. Furthermore, it is suggested that the enriched CH₄ in Bannock and Urania Basins is diffused and mixed with the overlying waters by local upward transport mechanisms that selectively move CH₄ upward in these Basins.     

Organic matter composition of the continental shelf and bathyal sediments of the Cretan Sea (NE Mediterranean)

The seasonal, spatial and bathymetric changes in the distribution of chloroplastic pigments (Chl a, phaeopigments and CPE), TOC, TON, ATP, bottom water nutrient content and the main biochemical classes of organic compounds (lipids, proteins and carbohydrates) were recorded from May 1994 to September 1995 over the continental margin of northern Crete. The concentration of chloroplastic pigment equivalents (CPE) was always low, dropping dramatically along the shelf-slope gradient. Microbial activity (ATP) also dropped sharply beyond the continental shelf following a distribution pattern similar to TOC and TON. Lipid, protein and carbohydrate concentrations, as well as biopolymeric carbon were comparable to those reported for other more productive areas, however, the quality of the organic matter itself was rather poor. Thus, carbohydrates, the dominant biochemical class, were characterised by being highly (80–99%) refractory, as soluble carbohydrates represented (on annual average) only 6% of the total carbohydrate pool. Protein and lipid concentrations strongly decreased with depth, indicating depletion of trophic resources in the bathyal zone. Proteins appeared to be the more degradable compounds and indeed the protein to carbohydrate ratios were found to decrease strongly in the deeper stations. Organic matter content and quality decreased both with increasing distance from the coast and within the sediment. All sedimentary organic compounds were found to vary between sampling periods, with the changes being more pronounced over the continental shelf. The different temporal patterns of the various components suggest a different composition and/or origin of the OM inputs during the different sampling periods. The amount of material reaching the sediments below 540 m is extremely low, suggesting that most of the organic material is decomposed and/or utilised before reaching the sea floor. In conclusion, the continental shelf and bathyal sediments of the Cretan Sea can be considered, from a trophic point of view, as two different subsystems.     

Lipid biomarkers and bacterial lipase activities as indicators of organic matter and bacterial dynamics in contrasted regimes at the DYFAMED site, NW Mediterranean

This study investigated the relationships between dissolved organic matter (DOM) composition and bacterial dynamics on short time scale during spring mesotrophic (March 2003) and summer oligotrophic (June 2003) regimes, in a 0–500 m depth water column with almost no advection, at the DYFAMED site, NW Mediterranean. DOM was characterized by analyzing dissolved organic carbon (DOC), colored dissolved organic matter (CDOM) and lipid class biotracers. Bacterial dynamic was assessed through the measurement of in situ bacterial lipase activity, abundance, production and bacterial community structure. We made the assumption that by coupling the ambient concentration of hydrolysable acyl-lipids with the measurement of their in situ bacterial hydrolysis rates (i.e. the free fatty acids release rate) would provide new insights about bacterial response to change in environmental conditions. The seasonal transition from spring to summer was accompanied by a significant accumulation of excess DOC (+5 μM) (ANOVA, p<0.05, n=8) in the upper layer (0–50 m). In this layer, the free fatty acids release rate to the bacterial carbon demand (BCD) ratio increased from 0.6±0.3 in March to 1.3±1.0 in June (ANOVA, p<0.05, n=8) showing that more uncoupling between the hydrolysis of the acyl-lipids and the BCD occurred during the evolution of the season, and that free fatty acids contributed to the excess DOC. The increase of lipolysis index and CDOM absorbance (from 0.24±0.17 to 0.39±0.13 and from 0.076±0.039 to 0.144±0.068; ANOVA, p<0.05, n=8, respectively), and the higher contribution of triglycerides, wax esters and phospholipids (from <5% to 12–31%) to the lipid pool reflected the change in the DOM quality. In addition to a strong increase of bacterial lipase activity per cell (51.4±29.4–418.3±290.6 Ag C cell⁻¹ h⁻¹), a significant percentage of ribotypes (39%) was different between spring and summer in the deep chlorophyll maximum (DCM) layer in particular, suggesting a shift in the bacterial community structure due to the different trophic conditions. At both seasons, in the chlorophyll layers, diel variations of DOM and bacterial parameters reflected a better bioavailability and/or DOM utilization by bacteria at night (the ratio of free fatty acids release rate to bacterial carbon demand decreased), most likely related to the zooplankton trophic behaviour. In mesotrophic conditions, such day/night pattern was driving changes in the bacterial community structure. In more oligotrophic period, diel variations in bacterial community structure were depth dependent in relation to the strong summer stratification.     

Particulate manganese and iron in recent sediments of the Gulf of Lions continental margin (north-western Mediterranean Sea): deposition and diagenetic process

This study aims at apprehending the major forcing factors which govern the spatial and vertical Mn and Fe distributions in ten cores from the Gulf of Lions. Speciation by chemical leaching experiments helps understanding their chemical behaviour during the early diagenetic processes in a marine environment under strong continental influence.The distribution and chemical behaviour of Mn and Fe differ drastically. The strong dependence of Mn distribution on chemical conditions is expressed by a marked solid phase Mn enrichment in the oxic layers of sediment deposits. This enrichment increases with decreasing sedimentation rate (increasing water depth). It is illustrated by an enhanced portion of total Mn in the reducible phase and the precipitation of todorokite on calcareous supports in the upper part of the deepest cores. As a result, 65% of the margin total particulate Mn is thought to be stored in slope deposits in a relatively strongly held association. Conversely, the low chemical mobility of iron in the sedimentary column is indicated by uniform depth profiles and an exclusive location in the residual chemical phase in all the studied sites. The Fe distribution is largely controlled by the dispersion of particulate material on the margin from the Rhône River main source.A budget of Mn and Fe accumulation based on the average amount of total Mn and Fe content of sediments deposited during the last 100 yr, has been made taking into account diagenetic readjustments. The total Mn and Fe amount stored on the margin during the last century is estimated to be 1.5×10⁶ and 54×10⁶ t, respectively. These values are seven times higher than the total secular external inputs calculated on the basis of present day riverine and atmospheric fluxes. This large discrepancy is mainly explained by the sharp decrease of the Rhone River discharge after dam buildings between 1950 and 1958. A residual discrepancy dating before 1950, is likely to be due to seafloor erosion and gravity induced mechanisms.     

A temporal analysis on the dynamics of deep-sea macrofauna: Influence of environmental variability off Catalonia coasts (western Mediterranean)

A seasonal analysis of deep-sea infauna (macrobenthos) based on quantitative sampling was conducted over the Catalan Sea slope, within the Besòs canyon (at ∼550-600 m) and on its adjacent slope (at 800 m). Both sites were sampled in February, April, June-July and October 2007. Environmental variables influencing faunal distribution were also recorded in the sediment and sediment/water interface. Dynamics of macrobenthos at the two stations showed differences in biomass/abundance patterns and trophic structures. Biomass was higher inside the Besòs canyon than on the adjacent slope. The community was mostly dominated by surface-deposit feeding polychaetes (Ampharetidae, Paraonidae, Flabelligeridae) and crustaceans (amphipods such as Carangoliopsis spinulosa and Harpinia spp.) inside the canyon, while subsurface deposit feeders (mainly the sipunculan Onchnesoma steenstrupii) were dominant over the adjacent slope. The taxonomic composition in the suprabenthic assemblages of polychaetes, collected on the adjacent slope by a suprabenthic sledge, was clearly different from that collected by the box-corer. The suprabenthic assemblage was dominated by carnivorous forms (mainly Harmothoe sp. and Nephthys spp.) and linked to higher near-bottom turbidity. Inside Besòs a clear temporal succession of species was related to both food availability and quality and the proliferation of opportunistic species was consistent with higher variability in food sources (TOC, C/N, ??¹³C) in comparison to adjacent slope. This was likely caused by a greater influence of terrigenous inputs from river discharges. Inside the canyon, Capitellidae, Spionidae and Flabelligeridae, in general considered as deposit feeders, were more abundant in June-July coinciding with a clear signal of terrigenous carbon (depleted ??¹³C, high C/N) in the sediments. By contrast, during October and under conditions of high water turbidity and increases of TOM, carnivorous polychaetes (Glyceridae, Onuphidae) increased. Total macrobenthos biomass found over Catalonian slopes were higher than that found in the neighboring Toulon canyon, probably because the two canyons are influenced by different river inputs, connected with distinct terrigenous sources.     

Total hydrolysable amino acid mineralisation in sediments across the northeastern Atlantic continental slope (Goban Spur)

Total hydrolysable amino acids (THAA), individual amino acid distributions, total organic carbon (TOC) and total nitrogen (TN) were measured in sediments across the Goban Spur continental slope at water depths of 651, 1296 and 3650 m. Objectives were to examine (1) differences in organic matter (OM) degradation state in surface sediments across the slope from sedimentary amino acid compositions, and (2) whether these differences are related to particle size distributions. Application of a ‘reaction–diffusion’ model to the sediment concentration profiles showed that TOC and THAA degradation rate constants decreased with increasing water depth. Ratios of degradation rate constants of THAA over TOC indicated that THAA turn over faster than TOC at 651 and 1296 m water depth only. From estimates of degradation rate constants of individual amino acids, it was concluded that with increasing water depth fewer amino acids contribute to overall THAA degradation. The contribution of THAA to TOC mineralisation decreased from the upper to the lower slope. Since at all three sampling stations the amino acids with the highest relative contribution to THAA had a higher abundance in sediments with reduced THAA mineralisation rates, we conclude that the overall amino acid reactivity decreases with increasing water column depth. A principal component analyses, carried out on normalised amino acid mole percentages, established significant shifts in amino acid compositions and confirmed that (1) OM degradation state increased from 651 to 3650 m and (2) that OM in the finest fraction at the shallowest station appeared to be considerably less degraded than in the coarser fractions or any size fractions at the deeper stations. Therefore, we conclude that downslope transport, sorting and accumulation of fine particles with continuous mineralisation of OM attached to the particles during vertical and lateral transport results in an increasing organic matter degradation state from the upper slope to the abyssal plain.     

Morphology and downslope sediment displacement in a deep-sea valley,the Valencia Valley (Northwestern Mediterranean)

The Valencia Valley is a Quaternary, 200 km long deep-sea valley in the Valencia Trough, Western Mediterranean Sea. A swathmapping survey approximately mid-way along the valley length, where the floor has an average gradient of 1:250 (0.2°), shows valley walls that rise 200 to 350 m above the valley floor, with slopes of 2 to 18°. Sediment forming the walls is undergoing retrogressive, upslope-directed slumping with increasing bedding disruption along steeper walls. The valley exhibits a winding course with steep outer and gentler inner walls around bends, and bedforms on the valley floor. Lateral migration around bends is less than 5 km and the valley is deeply entrenched into Quaternary-bedded sediments.     

南日群岛东部海域岩芯沉积物有机碳含量和δ13CTOC值的变化特征及古气候环境意义

2008年在南日群岛东部海域用钻机采集长度为1 004 cm的岩芯样品,测试沉积物中总有机碳（TOC）含量、稳定碳同位素比值（δ13CTOC）、碳氮比值（C/N）、碳酸盐含量以及14C数据,分析其垂直剖面上的变化特征,阐明其沉积环境和古气候的指示意义.沉积物TOC含量变化范围为0.44%～0.74%,垂直剖面上总体显示出末次冰期和全新世中期TOC含量较高,全新世早期相对稳定,而全新世中后期TOC波动范围明显增大.C/N值在5.3～14.9之间变化,平均值为8.1;δ13CTOC值范围为-23.07‰～-21.04‰,表明沉积物中的有机质以海洋自生为主,同时混有部分陆源有机质.碳酸盐含量在3.3%～10.9%之间.TOC含量、C/N比值、δ13CTOC值在岩芯垂直剖面上具有很好的相关性.14C测年数据以及各要素测值在垂直剖面上的变化特征表明：自11 250 aBP以来该海域附近古气候有可能经历了以下4个演化阶段：①11 500～9 000 aBP期间气候寒冷、海平面下降,采样点可能为浅水区或潮间带;②9 000～8 000 aBP期间为升温期,气候由寒冷转向温暖;③8 000～4 300 aBP期间,为相对稳定的暖湿期,在后期气候出现小幅的波动;④4 300 aBP至今,气候进入明显的周期性波动期,暖湿和干冷气候多次交替变化;4 300～3 000 aBP期间为相对寒冷的时期;3 000～1 800 aBP期间为较稳定温暖期,1 800～1 000 aBP期间气温快速降低,标志着研究区进入一个新的寒冷阶段,但是自1 000 aBP以来,气候又再次出现转暖的趋势.     

Bannock Basin,Sirte Abyssal Plain and Conrad Spur: structural relationships between Mediterranean Ridge and its western foreland and implications on the character of the accretionary complex (eastern Mediterranean)

In previous publications, the relationship between the Sirte Abyssal Plain as foreland and the Mediterranean Ridge as accretionary complex was considered to be simple: the foreland is undeformed, the accretionary complex consumes the foreland, the Messinian evaporites control the internal structure of the growing complex. The compilation of our own and published data results in a more complex tectonic pattern and a new geodynamic interpretation. The Sirte Abyssal Plain is imprinted by extensional tectonics which originated independently from and prior to the approaching process of accretion. The structural setting of the pre-Messinian and Messinian Sirte Abyssal Plain is responsible for the highly variable thickness of Messinian evaporites. The foreland setting in the Sirte Abyssal Plain also controls the internal structure of the Mediterranean Ridge, at least between the deformation front and Bannock Basin, following sediment deformation within the accretionary wedge with a dominating inherited SW-NE orientation. The taper angle of the post-Messinian Mediterranean Ridge is unusually small compared with other accretionary wedges. In the studied area, within a distance of about 45 km from the deformation front, there is no appreciable dip in the décollement. Therefore, the slope of the outer 45 km of the Mediterranean Ridge is considered to be caused only by gravitational spreading of Messinian evaporites deposited on the slope of pre-Messinian accretionary wedge. As a consequence, the Mediterranean Ridge underlying such slope is interpreted to belong to the foreland. The allochthonous evaporites overlie autochthonous evaporites of the Sirte Abyssal Plain. The NE-dipping décollement (and thus of the true tectonically driven deformation front) is expected to initiate at about the present position of Bannock Basin. The Sirte Abyssal Plain, the adjacent Cyrene Seamount and neighbouring seafloor relief on the African continental margin are considered to be the product of tectonic segmentation of the continental crust.     

Spatial and temporal distribution patterns of suspended particulate matter and particulate organic carbon in the Saronikos Gulf (eastern Mediterranean, Greece)

In 1998–1999, beam attenuation coefficient (bac) profiles, suspended particulate matter (SPM) and particulate organic carbon (POC) concentrations were assessed during five cruises in the Saronikos Gulf, eastern Mediterranean, Greece. SPM and POC concentrations (0.05–1.84 mg l⁻¹ and 10.2–468.6 μg l⁻¹, respectively) exhibited strong spatial and temporal variations, related to the different environmental characteristics of various sectors of the gulf, including wind regime and biological productivity. The Elefsis and Keratsini bays, as well as the area around Psyttaleia Island, showed the highest POC concentrations. The vertical distribution of POC at stations in the western basin, as well as in the inner and outer Saronikos Gulf is characterised by higher POC concentrations in surface waters, associated with higher biological activity. The wastewater treatment plant effluents discharged south of the Psyttaleia Island are a major source of organic particles which directly influence the intermediate water layers, at least during the stratification period. Assessments of relationships between bac and SPM or POC concentrations revealed a relatively strong correlation between bac and POC. An equation converting bac readings to POC concentration was established which can be applied to historical and/or future bac measurements, independently of season. POC concentrations estimated from calibrated continuous transmissometer readings were used to estimate the standing stock of POC in the Saronikos Gulf, which varied between 6,110×10⁶ and 13,450×10⁶ g C during the period June 1998 to February 1999.     

Pseudo-nitzschia spp. (Bacillariophyceae) and dissolved organic matter (DOM) dynamics in the Ebro Delta (Alfacs Bay, NW Mediterranean Sea)

Samples were collected during one annual cycle (April 2007–March 2008) at Alfacs Bay (NW Mediterranean Sea) central station in order to assess the influence of organic nutrients in the growth of the microalgae assemblage, with special reference to Pseudo-nitzschia spp. This potentially toxic diatom forms natural and recurrent blooms in the study area. To assess further the relationship between Pseudo-nitzschia spp. and nutrients an enrichment experiment with high molecular weight dissolved organic matter (HMWDOM) was performed with field samples obtained during a Pseudo-nitzschia spp. bloom. HMWDOM was extracted from water collected at Alfacs Bay. Five bioassays were prepared: N + P (seawater with addition of nitrate and phosphate), DOM (addition of HMWDOM), (−N + P) + DOM (nitrogen deficient, with addition of phosphate and HMWDOM), (N + P) + DOM (addition of nitrate, phosphate and HMWDOM), seawater control (without added nutrients), and B + DOM (control of bacteria, without microalgae). The experiment was run in batch mode over 4 days. Results from the field study revealed that the concentrations of organic nutrients mostly surpassed the inorganic pool. Pseudo-nitzschia spp. was the most frequent and abundant taxa of the microalgae community. The micro-planktonic assemblage was arranged according to a seasonal factor (ANOSIM and cluster analysis). DON, nitrate and silicate were the most important abiotic parameters contributing to the dissimilarities between seasons (SIMPER analysis) and thereby potentially influencing the seasonal distribution of microalgae in the representative station. In the experimental investigation, Pseudo-nitzschia cells increased by the end of the experiment in the DOM bioassay but no respective increase was observed for chlorophyll a. This could point to an acquisition of nutrients through the DOM fraction that would conjointly reduce the need of chlorophyll a. The data obtained suggest that organic nutrients may exert an important role in the development of microalgae, including Pseudo-nitzschia spp., in the selected location.     

Authigenic carbonates related to active seepage of methane-rich hot brines at the Cheops mud volcano,Menes caldera (Nile deep-sea fan,eastern Mediterranean Sea)

On the passive margin of the Nile deep-sea fan, the active Cheops mud volcano (MV; ca. 1,500 m diameter, ~20–30 m above seafloor, 3,010–3,020 m water depth) comprises a crater lake with hot (up to ca. 42 °C) methane-rich muddy brines in places overflowing down the MV flanks. During the Medeco2 cruise in fall 2007, ROV dives enabled detailed sampling of the brine fluid, bottom lake sediments at ca. 450 m lake depth, sub-surface sediments from the MV flanks, and carbonate crusts at the MV foot. Based on mineralogical, elemental and stable isotope analyses, this study aims at exploring the origin of the brine fluid and the key biogeochemical processes controlling the formation of these deep-sea authigenic carbonates. In addition to their patchy occurrence in crusts outcropping at the seafloor, authigenic carbonates occur as small concretions disseminated within sub-seafloor sediments, as well as in the bottom sediments and muddy brine of the crater lake. Aragonite and Mg-calcite dominate in the carbonate crusts and in sub-seafloor concretions at the MV foot, whereas Mg-calcite, dolomite and ankerite dominate in the muddy brine lake and in sub-seafloor concretions near the crater rim. The carbonate crusts and sub-seafloor concretions at the MV foot precipitated in isotopic equilibrium with bottom seawater temperature; their low δ¹³C values (–42.6 to –24.5‰) indicate that anaerobic oxidation of methane was the main driver of carbonate precipitation. By contrast, carbonates from the muddy lake brine, bottom lake concretions and crater rim concretions display much higher δ¹³C (up to –5.2‰) and low δ¹⁸O values (down to –2.8‰); this is consistent with their formation in warm fluids of deep origin characterized by ¹³C-rich CO₂ and, as confirmed by independent evidence, slightly higher heavy rare earth element signatures, the main driver of carbonate precipitation being methanogenesis. Moreover, the benthic activity within the seafloor sediment enhances aerobic oxidation of methane and of sulphide that promotes carbonate dissolution and gypsum precipitation. These findings imply that the coupling of carbon and sulphur microbial reactions represents the major link for the transfer of elements and for carbon isotope fractionation between fluids and authigenic minerals. A new challenge awaiting future studies in cold seep environments is to expand this work to oxidized and reduced sulphur authigenic minerals.     

Mass mortality of the fan mussel,Pinna nobilis in Turkey (eastern Mediterranean)

This study reported the mass mortality events (MMEs) of Pinna nobilis based on diving surveys in Turkey. Data were collected across 12 sites in the Aegean Sea, and one site in the Sea of Marmara, within the period of June-September 2019. The results showed that a low mortality rate of 10% was found in the Sea of Marmara, and a MME was not detected for this area. In contrast, 97% mortality was recorded for the P. nobilis population distributed in the Turkish Aegean Sea. This discrepancy might be related to the seawater temperature and salinity values of these two seas. The waters of the Sea of Marmara were colder and less salty than the Aegean Sea. The findings highlighted the importance of protecting the healthy population of critically endangered fan mussels in the Sea of Marmara. It was recommended that sound management plans comprising of monitoring, enforcement and public awareness activities could be implemented in order to achieve this.     

Seasonal dynamics in colored dissolved organic matter in the Mediterranean Sea: Patterns and drivers

Two autonomous profiling “Bio-Argo” floats were deployed in the northwestern and eastern sub-basins of the Mediterranean Sea in 2008. They recorded at high vertical (1 m) and temporal (5 day) resolution, the vertical distribution and seasonal variation of colored dissolved organic matter (CDOM), as well as of chlorophyll-a concentration and hydrological variables. The CDOM standing stock presented a clear seasonal dynamics with the progressive summer formation and winter destruction of subsurface CDOM maxima (YSM, for Yellow Substance Maximum). It was argued that subsurface CDOM is a by-product of phytoplankton, based on two main characteristics, (1) the YSM was located at the same depth than the deep chlorophyll maximum (DCM) and (2) the CDOM increased in summer parallels the decline in chlorophyll-a. These observations suggested an indirect but tight coupling between subsurface CDOM and phytoplankton via microbial activity or planktonic foodweb interactions. Moreover, the surface CDOM variations observed both by floats and MODIS displayed different seasonal dynamics from what recorded at subsurface one. This implies that CDOM standing stock can be hardly detected by satellite. It is worthnoting that surface CDOM was found to be more related to the sea surface temperature (SST) than chlorophyll-a concentration, suggesting its physical origin, in contrast to the biological origin of YSM and subsurface standing stocks.     

Seasonal dynamics of light absorption by chromophoric dissolved organic matter (CDOM) in the NW Mediterranean Sea (BOUSSOLE site)

We analyze a two-year time-series of chromophoric dissolved organic matter (CDOM) light absorption measurements in the upper 400 m of the water column at the BOUSSOLE site in the NW Mediterranean Sea. The seasonal dynamics of the CDOM light absorption coefficients at 440 nm (a_cdom(440)) is essentially characterized by (i) subsurface maxima forming in spring and progressively reinforcing throughout summer, (ii) impoverishment in the surface layer throughout summer and (iii) vertical homogeneity in winter. Seasonal variations of the spectral dependence of CDOM absorption, as described by the exponential slope value (S_cdom), are characterized by highest values in summer and autumn at the surface and low values at the depths of a_cdom(440) subsurface maxima or just below them. Variations of a_cdom(440) are likely controlled by microbial digestion of phytoplankton cells, which leads to CDOM production, and by photochemical destruction (photobleaching), which leads to CDOM degradation. Photobleaching is also the main driver of S_cdom variations. Consistently with previous observations, a_cdom(440) for a given chlorophyll a concentration is higher than expected from Case I waters bio-optical models. The total non-water light absorption budget shows that surface waters at the BOUSSOLE site are largely dominated by CDOM during all seasons but the algal bloom in March and April. These results improve the knowledge of CDOM absorption dynamics in the Mediterranean Sea, which is scarcely documented. In addition, they open the way to improved algorithms for the retrieval of CDOM absorption from field or satellite radiometric measurements.     

Stable isotopes in deep-sea living (stained) foraminifera from the Mozambique Channel (eastern Africa): multispecies signatures and paleoenvironmental application

Oxygen and carbon isotopes (δ¹⁸O and δ¹³C) have been investigated in carbonate tests of deep-sea foraminifera living in the Mozambique Channel (eastern Africa) to understand how environmental constraints (e.g., organic matter, oxygenation) control the intra- and interspecific variability of isotopic signatures. 197 living individuals, including eight different species, from various microhabitats within the sediment were sorted from sediment samples gathered at two stations on the Malagasy upper slope. Results show that the δ¹⁸O values of foraminiferal taxa were not controlled by microhabitat pattern. They presented tremendous and intriguing intraspecific variability that is not explained by the classical ontogenetic effect. The δ¹³C values of infaunal foraminiferal taxa do not show a 1:1 relationship with the bottom water δ¹³C DIC and do not present a constant offset from it; instead, they appear to be mainly controlled by a microhabitat effect. The lower δ¹³C values of shallow, intermediate, and deep infaunal taxa at the deeper station compared to those seen at the shallower station reflect the enhanced exportation of sedimentary organic matter at the sediment–water interface, and its related mineralization within the upper sediments. The ?δ¹³C between shallow/very shallow infaunal species (i.e., Hoeglundina elegans, Uvigerina hispida) and intermediate/deep infaunal species (i.e., Melonis barleeanus, Globobulimina barbata) permits insight into (1) the exportation of organic matter to the seafloor and (2) the various degradation pathways for organic detritus in the benthic environments off NW Madagascar.     

Diffusive and total oxygen uptake of deep-sea sediments in the eastern South Atlantic Ocean:in situ and laboratory measurements

Total O₂ uptake rates were measured by the benthic flux chamber lander ELINOR, and O₂ microprofiles were measured by the profiling lander PROFILUR in the eastern South Atlantic. Diffusive O₂ fluxes through the diffusive boundary layer and the depth distribution of O₂ consumption rates within the sediment were calculated from the obtained microprofiles. The depth integrated O₂ consumption rate agreed closely with the diffusive O₂ uptake at all stations. Total O₂ uptake was 1.2–4.2 times the diffusive O₂ uptake, and the difference correlated with the abundance of macrofauna in the sediment. Diffusive O₂ uptake and O₂-penetration depths correlated with the organic content of the sediments and exhibited an inverse correlation with water depth. Total and diffusive rates of in situ O₂ uptake were higher than previously published data for shelf and abyssal sediments in the Atlantic, but were comparable to rates from upwelling areas in the eastern Pacific. Laboratory measurements on recovered sediment cores showed lower O₂ penetration depths and higher diffusive uptake rates than in situ measurements. The differences increased with increasing water depth. We primarily ascribe this compression of O₂ profiles to a transiently increased temperature during recovery and enhanced microbial activity in decompressed sediment cores. Total O₂ uptake rates measured in the laboratory on macrofauna-rich stations were, in contrast, lower than those measured in situ because of underrepresentation and disturbance of the macrofauna.     

Dioxin compounds in the deep-sea rose shrimp Aristeus antennatus (Risso, 1816) throughout the Mediterranean Sea

Polychlorodibenzo-p-dioxins (PCDDs) and polychlorodibenzofurans (PCDFs) are among the more toxic anthropogenic contaminants. They are fat-soluble and accumulate in animal tissues. Exposure to PCDD/Fs can cause several endocrine, reproductive and developmental problems in animals, including human beings. Several studies have demonstrated that fish and invertebrates living in association with sediments are exposed to and accumulate contaminants, but to date there have been no studies of PCDD/Fs contamination in deep-sea regions. Specimens of Aristeus antennatus (Risso, 1816) were collected from depths of 600–2500 m at different points in the Mediterranean Sea, from the western basin off the coast of Barcelona to the central basin off the Peloponnesian Peninsula, with otter trawl gear. Amounts of PCDD/Fs were measured in different animal tissues by high resolution gas chromatography coupled to high resolution mass spectrometry (HRGC–HRMS). This is the first study to report the presence of PCDD/Fs in deep-sea organisms dwelling at depths below 600 m. A. antennatus presented levels of PCDD/Fs of the same order of magnitude, or slightly higher, as those found in shallow-water species (Melicertus kerathurus) with respect to land-generated contamination. This highlights the widespread distribution of these pollutants and the potential threat posed to the biodiversity of fragile and vulnerable ecosystems such as the deep-sea. PCDD/F levels detected in the edible parts (muscle) of the commercial shrimp A. antennatus were clearly below the toxic limit value established by European legislation. Levels followed the trend muscle<ovary<hepatopancreas, according to the lipid content of the organs in question. The specific profile of toxic PCDD/F congeners in the studied Penaeid shrimp specimens was characterized mainly by octachlorodibenzo-p-dioxin (OCDD), 2,3,7,8-tetrachlorodibenzofuran (TCDF) and 2,3,4,7,8-pentachlorodibenzofuran (PeCDF) congeners. Moreover, non 2,3,7,8-substituted congeners bioaccumulated in this species, as observed in shallow-water crustaceans. PCDD/Fs were found in the shrimp A. antennatus throughout the Mediterranean Sea. Total PCDD/Fs burdens were higher in shrimps caught in the western Mediterranean than in those caught at eastern Mediterranean sites. There was a tendency for higher levels of PCDD/F contamination in samples obtained from deeper (2500 m) than from shallower sites (600 m).     

Geographical variation in metazoan parasites of the deep-sea fish Bathypterois mediterraneus Bauchot, 1962 (Osteichthyes: Ipnopidae) from the Western Mediterranean

This study examines the parasite fauna of Bathypterois mediterraneus, the most common fish below 1500 m in Western Mediterranean waters. Samples were obtained during July 2010 from the continental slope of two different areas (off Catalonia and Balearic Islands) in three different bathymetric strata at depths between 1000 and 2200 m. The parasite fauna of B. mediterraneus included a narrow range of species: Steringophorus cf. dorsolineatum, Scolex pleuronectis, Hysterothylacium aduncum, Anisakis sp. larva 3 type II and Sarcotretes sp. Steringophorus cf. dorsolineatum and H. aduncum were the most predominant parasites. H. aduncum showed significant differences in abundance between depths of 2000–2200 m with 1000–1400 m and 1400–2000 m, irrespective of locality, whereas S. cf. dorsolineatum showed significant differences between the two localities at all depths except for 2000–2200 m. We suggest the possible usefulness of these two parasites as geographical indicators for discriminating discrete stocks of B. mediterraneus in Western Mediterranean waters.     

Reprint of: Comparison of modern fluid distribution,pressure and flow in sediments associated with anticlines growing in deepwater (Brunei) and continental environments (Iran)

Differences in fluids origin, creation of overpressure and migration are compared for end member Neogene fold and thrust environments: the deepwater region offshore Brunei (shale detachment), and the onshore, arid Central Basin of Iran (salt detachment). Variations in overpressure mechanism arise from a) the availability of water trapped in pore-space during early burial (deepwater marine environment vs arid, continental environment), and b) the depth/temperature at which mechanical compaction becomes a secondary effect and chemical processes start to dominate overpressure development. Chemical reactions associated with smectite rich mud rocks in Iran occur shallow (∼1900 m, smectite to illite transformation) causing load-transfer related (moderate) overpressures, whereas mechanical compaction and inflationary overpressures dominate smectite poor mud rocks offshore Brunei. The basal detachment in deepwater Brunei generally lies below temperatures of about 150 °C, where chemical processes and metagenesis are inferred to drive overpressure development. Overall the deepwater Brunei system is very water rich, and multiple opportunities for overpressure generation and fluid leakage have occurred throughout the growth of the anticlines. The result is a wide variety of fluid migration pathways and structures from deep to shallow levels (particularly mud dykes, sills, laccoliths, volcanoes and pipes, fluid escape pipes, crestal normal faults, thrust faults) and widespread inflationary-type overpressure. In the Central Basin the near surface environment is water limited. Mechanical and chemical compaction led to moderate overpressure development above the Upper Red Formation evaporites. Only below thick Early Miocene evaporites have near lithostatic overpressures developed in carbonates and marls affected by a wide range of overpressure mechanisms. Fluid leakage episodes across the evaporites have either been very few or absent in most areas. Locations where leakage can episodically occur (e.g. detaching thrusts, deep normal faults, salt welds) are sparse. However, in both Iran and Brunei crestal normal faults play an important role in the transmission of fluids in the upper regions of folds.