Foraminifera,testate amoebae and diatoms as sea‐level indicators in UK saltmarshes: a quantitative multiproxy approach

The vertical distribution of foraminifera, testate amoebae and diatoms was investigated in saltmarshes in the Taf estuary (south Wales), the Erme estuary (south Devon) and the Brancaster marshes (north Norfolk), to assess the use of multiproxy indicators in sea‐level reconstructions. A total of 116 samples were subjected to regression analyses, using the program calibrate, with duration of tidal flooding as the dependent variable. We found that the relationship between flooding duration and taxa was strongest for diatoms and testate amoebae and weakest for foraminifera. The vertical range of testate amoebae in saltmarshes is small. Their lower tolerance limit in present‐day saltmarshes occurs where tides cover the marsh less than a combined total of 7 days (1.9%) in a year. However, they are important sea‐level indicators because information for sea‐level reconstruction is best derived from sediments that originate in the highest part of the intertidal zone. Diatoms span the entire sampled range in intertidal and supratidal areas, whereas the upper limit of foraminifera is found very close to the highest astronomical tide level. Local training sets provide reconstructions with higher accuracy and precision than combined training sets, but their use is limited if they do not represent adequate modern analogues for fossil assemblages. Although analyses are time consuming, a regional training set of all three groups of micro‐organisms yields highly accurate (r² = 0.80) and precise (low value of root mean square error) predictions of tidal level. This approach therefore could improve the accuracy and precision of Holocene sea‐level reconstructions. Copyright © 2001 John Wiley & Sons, Ltd.     

Analysis of fossil testate amoebae in Selisoo Bog,Estonia: local variability and implications for palaeoecological reconstructions in peatlands

Niinemets, E., Pensa, M. & Charman, D. J. 2010: Analysis of fossil testate amoebae in Selisoo Bog, Estonia: local variability and implications for palaeoecological reconstructions in peatlands. Boreas, 10.1111/j.1502‐3885.2010.00188.x. ISSN 0300‐9483. Local variability in decadal water‐table changes on an ombrotrophic peatland was explored using testate amoebae analysis of near‐surface peats in an Estonian raised bog. The distribution of testate amoebae assemblages was studied along the gradient from hummock to hollow in the upper 30‐cm layer of peat. As expected, testate amoebae assemblages in different micro‐ecotypes from hummock to hollow, even as close as 10 m apart, are distinctly different. Past water‐table change was reconstructed by applying a transfer function based on modern samples from throughout Europe. Results show a decline in water level from the mid‐late 20th century on Selisoo bog in all profiles from the different micro‐ecotypes. However, the absolute water‐table depths and amplitudes of fluctuations vary between reconstructions from different sampling micro‐ecotypes. Cores were correlated using changes in non‐mire pollen concentrations down‐core, but it was not possible to correlate minor changes in water‐table owing to non‐contiguous sampling and variable accumulation rates. We conclude that different microtopes show the same decadal trends in relative water‐table change but that the absolute magnitude of change may be more variable locally. It is important that reconstructed palaeohydrological changes in bogs consider changes in bog micro‐ecotypes, and their variation over time, as this may alter the sensitivity of an individual record to drivers such as climate change. Comparison and compilation of data from parallel cores from different micro‐ecotypes and/or different sites are likely to provide more robust reconstructions.     

Can testate amoeba‐based palaeohydrology be extended to fens?

Numerous palaeoecological studies have used testate amoeba analysis to reconstruct Holocene hydrological change in peatlands, and thereby past climatic change. Current studies have been almost exclusively restricted to ombrotrophic bogs and the period since the fen–bog transition. Although the critical link between peatland surface wetness and climate is less direct in minerotrophic peatlands, such records may still be of value where there are few others, particularly if multiple records can be derived and inter‐compared. Expanding the temporal and spatial scope of testate amoeba‐based palaeohydrology to minerotrophic peatlands requires studies to establish the primacy of hydrology and the efficacy of transfer functions across a range of sites. This study analyses testate amoeba data from wetlands spanning the trophic gradient in the eastern Mediterranean region. Results demonstrate that different types of wetlands have distinctly different amoeba communities, but hydrology remains the most important environmental control (despite water table depth being measured at different times for different sites). Interestingly, Zn and Fe emerge as significant environmental variables in a subset of sites with geochemical data. Testate amoeba–hydrology transfer functions perform well in cross‐validation but frequently perform poorly when applied to other sites, particularly with sites of a different nutrient status. It may be valid to use testate amoebae to reconstruct hydrological change from minerotrophic peatlands with an applicable transfer function; however, it may not be appropriate to use testate amoebae to reconstruct hydrological change through periods of ecosystem evolution, particularly the fen–bog transition. In practice, the preservation of amoeba shells is likely to be a key problem for palaeoecological reconstruction from fens. Copyright © 2010 John Wiley & Sons, Ltd.     

Holocene changes on a peatland in northwestern Ontario interpreted from testate amoebae (Protozoa) analysis

Radiocarbon-dating and analyses of fossil testate amoebae (Protozoa) have established changes in soil moisture conditions on the developing surface of a Sphagnum -dominated peatland near Emo in northwestern Ontario.
The distribution and composition of modern testate amoebae communities were studied from peatlands in the region of Ontario and Minnesota as a guide to interpreting fossil assemblages. Although the core spans all of the Holocene, fossil testate amoebae were recovered only from the part post-dating 6500 BP. Earliest testate amoebae assemblages associated with bryophytic and cyperaceous-rich fen peat are dominated by species in the genera Cyclopyxis and Centropyxis . By 5000 BP, Amphitrema Jraaum, Assulina muscorum, Heleopera sphugni and Hyalosphenia subjaoa become important species as Sphagnum-rich peat accumulated at the site. Present-day microtopographic differentiation probably developed during historic time when the site became progressively drier, as indicated by a change of Nebela griseola, N. militaris and Trigonopyxis arcula . Although it is possible to derive quantitative estimates of changing soil moisture conditions from testate amoebae, care should be taken in interpreting results, particularly from non-Sphagnum-rich peats, until more is learned about the distribution and ecology of modern faunas.     

Response of testate amoeba assemblages to environmental and climatic changes during the Lateglacial–Holocene transition at Lake Lautrey (Jura Mountains,eastern France)

We tested the response of lacustrine testate amoebae (thecamoebians) to climate and environmental changes for the Lateglacial–Holocene transition. The palaeoenvironmental history of the study site (Lake Lautrey, Jura Mountains, eastern France) was previously established based on high‐resolution multi‐proxy studies of the same core. The present study is characterised by a high taxonomic resolution (54 taxa), inclusion of small species (down to 25 µm) and high total counts (>500 individuals per sample on average). Changes in the composition of testate amoeba assemblages (dominant species and assemblage structure), as well as in the accumulation rate (tests cm^?2 a^?1), corresponded to major climatic phases (i.e. Oldest Dryas, Bølling–Allerød Interstadial, Younger Dryas, Preboreal) as well as changes in organic matter inputs. Furthermore, decreases in the accumulation rate characterised minor short‐lived cooling events, such as Older Dryas event or Gerzensee oscillation. However, the Preboreal oscillation, which was well registered by other proxies at Lake Lautrey, could not be recognised in the testate amoeba record. This work demonstrates that lacustrine testate amoebae can be used for palaeoclimatic and palaeoecological reconstructions. Nevertheless, a better understanding of the relation between climate, organic matter and lacustrine testate amoebae requires further high‐resolution studies based on multi‐proxy approaches and the development of appropriate modern analogues. Copyright © 2010 John Wiley & Sons, Ltd.     

The Holocene shallowing‐upward parasequence of north‐west Andros Island,Bahamas

Many pre‐Mesozoic records of Earth history are derived from shallow water carbonates deposited on continental shelves. While these carbonates contain geochemical proxy records of climate change, it is the stratal architecture of layered carbonate units that often is used to build age models based on the idea that periodic astronomical forcing of sea‐level controls the layering. Reliable age models are crucial to any interpretation of rates and durations of environmental change, but the physical processes that actually control this stratal architecture in shallow water carbonates are controversial. In particular, are upward‐shallowing stacks of carbonate beds bounded by flooding surfaces (‘parasequences’) truly a record of relative sea‐level change? The purpose of this study is to examine a tidal flat that is actively accumulating carbonate stratigraphy, and to determine the relative importance of tidal channel migration (poorly known, but investigated here) and Holocene sea‐level rise (well‐known) in controlling post‐glacial parasequence architecture. This work represents a field study of peritidal carbonate accumulation at Triple Goose Creek, north‐west Andros Island. By integrating surface facies maps with differential global positioning system topographic surveys, a quantitative relationship between facies and elevation is derived. Sedimentary facies are sensitive to elevation changes as small as 5 cm, and are responding to both internal (distance to nearest tidal channel) and external (sea‐level rise) controls. The surface maps also are integrated with 187 sediment cores that each span the entire Holocene succession. While flooding of the Triple Goose Creek area should have occurred by ca 4500 years ago, preservation of Holocene sediment did not begin until 1200 years ago. The tidal channels are shown to be stationary, or to migrate sluggishly at up to 6 cm per year. Therefore, while the location of tidal channels is responsible for the modern mosaic of surface facies, these facies and the channels that control them have not migrated substantially during the ca 1200 years of sediment accumulation at Triple Goose Creek. Once the region was channellized, vertical and lateral shifts in facies, such as the landward retreating shoreline, expanding mangrove ponds and seaward advancing inland algal marsh, are driven by changes in relative sea‐level and sediment supply, not migrating channels. While stratigraphic columns look different depending on the distance to the nearest tidal channel, the overall parasequence architecture everywhere at Triple Goose Creek records an upward‐shallowing trend controlled by the infilling of accommodation space generated by post‐glacial sea‐level rise.     

长白山区泥炭地现代有壳变形虫环境意义探讨

有壳变形虫(testate amoebae)是一种新的具有潜力的环境变化生物指标。对采集自长白山区哈泥(42°12′50″N,126°31′05″E)、金川(42°20′47″N,126°21′35″E)、赤池(42°03′16″N,128°03′22″E)和圆池(42°01′55″N,128°25′58″E)等4个泥炭地不同生境的75个有壳变形虫样品,采用冗余分析方法(RDA)研究有壳变形虫种类组合变化与7个环境变量的关系,所有采样点均以泥炭藓(Sphagnum)为优势植被。结果表明水位埋深(depth to water table),pH值和泥炭湿度是影响长白山区泥炭地有壳变形虫种类变化的主要环境因子,显著性检验达到p＜0.001的水平。这一结果与国外其他地区的研究结果相一致,这3个环境因子可以作为目标变量进行有壳变形虫－环境因子转换函数的构建。     

Testate amoebae as proxies for mean annual water‐table depth in Sphagnum‐dominated peatlands of North America

Peatland‐inhabiting testate amoebae are sensitive indicators of substrate‐moisture conditions and have increasingly been used in palaeohydrological studies. However, to improve accuracy of testate‐amoeba‐based hydrological inferences, baseline ecological data on rare taxa, a larger geographic network of calibration sites, and incorporation of long‐term estimates of water‐table depth are needed. Species–environment relationships at 369 sites from 31 peatlands in eastern North America were investigated. Long‐term estimates of water‐table depth were obtained using the method of polyvinyl (PVC) tape‐discolouration. Transfer functions were developed using a variety of models, and validated through jackknifing techniques and with an independent dataset where water‐table depths were directly measured throughout the growing season. Results indicate that mean annual water‐table depth can be inferred from testate amoeba assemblages with a mean error of 6 to 8 cm, although there is a slight systematic bias. All transfer function models performed similarly and produced similar reconstructions on a fossil sequence. In a preliminary effort towards development of a comprehensive North American calibration dataset, data from this study were combined with previous studies in Michigan and the Rocky Mountains (n = 650). This combined dataset had slightly larger mean errors of prediction (8–9 cm) but includes data for several rare taxa. Copyright © 2007 John Wiley & Sons, Ltd.     

Reconstruction of recent sea-level change using testate amoebae

Proxy-based sea-level reconstructions place the instrumentally observed rates of recent sea-level rise in a longer term context by providing data that extend the instrumental sea-level record into past centuries. This paper presents the first sea-level reconstructions based on analyses of testate amoebae, to test their ability to produce high-precision reconstructions of past sea level. We present two reconstructions for the past 100 yr from sites in Maine (USA) and Nova Scotia (Canada) based on short cores from salt marshes, and modern training data from North America and the United Kingdom. These are compared with tide-gauge records and reconstructions based on foraminifera from the same cores. The reconstructions show good agreement with both the tide-gauge data and the foraminifera-based reconstructions. The UK data perform well in predicting known elevations of North American surface samples and produce sea-level reconstructions very similar to those based on the North American data, suggesting the methodology is robust across large geographical areas. We conclude that testate amoebae have the potential to provide robust, higher precision sea-level reconstructions for the past few centuries if modern transfer functions are improved and core sites are located within the main zone of testate amoebae occurrence on the salt marsh.     

A near‐annual palaeohydrological study based on testate amoebae from a sub‐alpine mire: surface wetness and the role of climate during the instrumental period

We present the first testate amoeba‐based palaeohydrological reconstruction from the Swiss Alps, and the first depth to the water table (DWT) calibration dataset for this region. Compared to existing models, our new calibration dataset performs well (RMSEP = 4.88), despite the length of the water table gradient covered (53 cm). The present‐day topography and vegetation of the study mire Mauntschas suggest that it is partly ombrotrophic (large Sphagnum fuscum hummocks, one of which was the coring site) but mostly under the minerotrophic influence of springs in the mire and runoff from the surrounding area. Ombrotrophic Sphagnum fuscum hummocks developed at the sampling site only during the last 50 years, when testate amoebae indicate a shift towards dry and/or acid conditions. Prior to AD 1950 the water table was much higher, suggesting that the influence of the mineral‐rich water prevented the development of ombrotrophic hummocks. The reconstructed DWT correlated with Pinus cembra pollen accumulation rates, suggesting that testate amoebae living on the mire and P. cembra growing outside of it partly respond to the same factor(s). Finally, temperature trends from the nearby meteorological station paralleled trends in reconstructed DWT. However, contrary to other studies made on raised bogs of northwestern Europe, the highest correlation was observed for winter temperature, despite the fact that testate amoebae would more logically respond to moisture conditions during the growing season. The observed correlation with winter temperature might reflect a control of winter severity on surface moisture during at least the first part of the growing season, through snow melt and soil frost phenomena influencing run‐off. More ecohydrological work on sub‐alpine mires is needed to understand the relationships between climate, testate amoebae and peatland development. Copyright © 2009 John Wiley & Sons, Ltd.     

Hydroperiod and its influence on nekton use of the salt marsh: A pulsing ecosystem

The salt marsh surface is not a homogeneous environment. Rather, it contains a mix of different microhabitats, which vary in elevation, microtopography, and location within the estuarine system. These attributes act in concert with astronomical tides and meteorological and climatological events and result in pulses of tidal flooding. Marsh hydroperiod, the pattern of flooding events, not only controls nekton access to marsh surface habitats directly but may also mediate habitat exploitation through its influence on other factors, such as prey abundance or vegetation stem density. The relative importance of factors affecting marsh hydroperiod differ between the southeast Atlantic and northern Gulf of Mexico coasts. Astronomical tidal forcing is the primary determinant of hydroperiod in Atlantic Coast marshes, whereas predictable tides are often overridden by meteorological events in Gulf Coast marshes. In addition, other factors influencing coastal water levels have a proportionately greater effect on the Gulf Coast. The relatively unpredictable timing of marsh flooding along the Gulf Coast does not seem to limit habitat utilization. Some of the highest densities of nekton reported from salt marshes are from Gulf Coast marshes that are undergoing gradual submergence and fragmentation caused by an accelerated rise in relative sea level. Additional studies of habitat utilization are needed, especially on the Pacific and Atlantic coasts. Investigations should include regional comparisons of similar microhabitats using identical quantitative sampling methods. Controlled field experiments are also needed to elucidate the mechanisms that affect the habitat function of salt marshes.     

Ecosystem Responses of a Tidal Freshwater Marsh Experiencing Saltwater Intrusion and Altered Hydrology

Tidal freshwater marshes exist in a dynamic environment where plant productivity, subsurface biogeochemical processes, and soil elevation respond to hydrological fluctuations over tidal to multi-decadal time scales. The objective of this study was to determine ecosystem responses to elevated salinity and increased water inputs, which are likely as sea level rise accelerates and saltwater intrudes into freshwater habitats. Since June 2008, in situ manipulations in a Zizaniopsis miliacea (giant cutgrass)-dominated tidal freshwater marsh in South Carolina have raised porewater salinities from freshwater to oligohaline levels and/or subtly increased the amount of water flowing through the system. Ecosystem-level fluxes of CO₂ and CH₄ have been measured to quantify rates of production and respiration. During the first 20 months of the experiment, the major impact of elevated salinity was a depression of plant productivity, whereas increasing freshwater inputs had a greater effect on rates of ecosystem CO₂ emissions, primarily due to changes in soil processes. Net ecosystem production, the balance between gross ecosystem production and ecosystem respiration, decreased by 55% due to elevated salinity, increased by 75% when freshwater inputs were increased, and did not change when salinity and hydrology were both manipulated. These changes in net ecosystem production may impact the ability of marshes to keep up with rising sea levels since the accumulation of organic matter is critical in allowing tidal freshwater marshes to build soil volume. Thus, it is necessary to have regional-scale predictions of saltwater intrusion and water level changes relative to the marsh surface in order to accurately forecast the long-term sustainability of tidal freshwater marshes to future environmental change.     

鄂西亚高山泥炭地有壳变形虫生态监测及对水位的指示意义

开展湿地的生态监测研究是理解当今气候变化背景下湿地的生态响应、动态演化和生态服务功能变化的有效途径,也是湿地保护、科学管理、合理规划和生态恢复的内在需求.以我国鄂西地区广泛分布的典型亚高山泥炭湿地为研究地点,以在湿地中大量生活且对环境变化敏感的环境指示生物——有壳变形虫为研究对象,采用了一种较新的泥炭湿地水位长期定点监测方法——"PVC印迹法"监测湿地水位的长期变化,调查了有壳变形虫的生物多样性、群落组合特征及其与水位等主要环境因子的响应关系,共记录到有壳变形虫27种和类群（type）,隶属14个属.对有壳变形虫的群落组成与环境因子的关系排序分析显示,泥炭湿地的水位是有壳变形虫群落组成的主控环境因子,构建了有壳变形虫属种与水位的转换函数,该函数具有较好的预测和推导能力（R2=0.62）,为该地区泥炭湿地的古水位重建提供了现代过程的数据支持.      

渤海湾沿岸贝壳堤对潮滩有孔虫海面变化指示意义的影响

通过对渤海湾沿岸有贝壳堤发育和无贝壳堤发育的2类现代开放潮滩有孔虫组合的对比,研究了该2类潮滩环境沉积物中有孔虫的海面指示意义。5个有孔虫组合带被MHWST(平均大潮高潮位)、MHW(平均高潮位)、MHWNT(平均大潮低潮位)和MSL(平均海面)分隔,分别对应潮滩的高盐沼、低盐沼、潮间带上部、潮间带中上部和潮间带中下部5个不同亚环境。不同亚环境沉积物因其所含的有孔虫群的独特性,均可作为高精度海面标志物,误差为各亚带高差的1/2。沿岸贝壳堤可对潮滩有孔虫的属种组成产生影响,影响程度从高潮位向低潮位逐渐降低。在MHWNT潮位之下,有孔虫组合不再受到沿岸贝壳堤的影响。     

Changes in saltmarsh surface elevation due to variability in evapotranspiration and tidal flooding

We examine the potential for diurnal variation in elevation of saltmarsh surfaces as a source of error in long-term experiments; errors particularly critical in high precision studies that employ the surface elevation table (SET) as a means to monitor elevations. The field study was carried out along the New Brunswick coast of the Bay of Fundy in high and low zones at three marshes with different tidal ranges. We used a total of 16 benchmark pipes and controlled for daily variability in evapotranspiration (ET), as well as timing of tidal flooding, two factors that affect soil water storage, and consequently soil volumes. In six of nine trials we detected significant elevation change over periods as short as 5 d. Marsh-wide averages ranged from 1.2 to 3.0 mm, greater than the yearly increase in relative sea level in many regions. Wood Point marsh had the highest tidal range, but lowest soil organic matter content, giving its soils the lowest compressibility and little sensitivity to ET during two of three trials; the average change in elevation in Wood Point high marsh stations was 4.0 mm during the last trial. Greater differences later in the growing season (while temperature changes were minor) at Wood Point and another site suggest that plant transpiration drove changes in water storage at those sites. Significantly greater differences in elevation with lower plant cover in the third marsh suggests that evaporation drove changes in water storage there. Surface elevation change due to ET should be of greatest concern to SET users in temperate regions where there are large changes in plant biomass and variable temperatures. Variation due to plant transpiration could be reduced if yearly monitoring is scheduled before the start of the growing season.     

The distribution and utility of sea‐level indicators in Eurasian sub‐Arctic salt marshes (White Sea,Russia)

In support of efforts to reconstruct relative sea level (RSL), we investigated the utility of foraminifera, diatoms and bulk‐sediment geochemistry (δ¹³C, C:N and parameters measured by Rock‐Eval pyrolysis) as sea‐level indicators in Eurasian sub‐Arctic salt marshes. At three salt marshes (<15 km apart) in Dvina Bay (White Sea, Russia), we collected surface sediment samples along transects from subtidal to Taiga forest environments. Foraminifera at all sites formed bipartite assemblages, where elevations below mean high higher water (MHHW) were dominated by Miliammina spp. and elevations between MHHW and the highest occurrence of foraminifera were dominated by Jadammina macrescens and Balticammina pseudomacrescens. Five high‐diversity groups of diatoms were identified and they displayed pronounced variability amongst the study sites. Bulk‐sediment geochemistry recognized two groups (clastic‐dominated environments below MHHW and organic‐rich environments above MHHW). As one group included subtidal elevations and the other included supratidal elevations, we conclude that the measured geochemical parameters are not stand‐alone sea‐level indicators. Core JT2012 captured a regressive sediment succession of clastic, tidal‐flat sediment overlain by salt‐marsh organic silt and freshwater peat. The salt‐marsh sediment accumulated at 2804±52 years before present and preserved foraminifera (Jadammina macrescens and Balticammina pseudomacrescens) with good analogy to modern assemblages indicating that RSL was +2.60±0.47 m at this time. Diatoms confirm that marine influence decreased through time, but the lack of analogy between modern and core assemblages limited their utility as sea‐level indicators. Geochemical parameters also indicate a reduction in marine influence through time. We conclude that RSL reconstructions derived from salt‐marsh sediment preserved beneath Eurasian sub‐Arctic peatlands can provide valuable insight into the spatio‐temporal evolution of the Fennoscandian and Eurasian ice sheets.     

The performance of single- and multi-proxy transfer functions (testate amoebae,bryophytes, vascular plants) for reconstructing mire surface wetness and pH

Peatlands are widely exploited archives of paleoenvironmental change. We developed and compared multiple transfer functions to infer peatland depth to the water table (DWT) and pH based on testate amoeba (percentages, or presence/absence), bryophyte presence/absence, and vascular plant presence/absence data from sub-alpine peatlands in the SE Swiss Alps in order to 1) compare the performance of single-proxy vs. multi-proxy models and 2) assess the performance of presence/absence models. Bootstrapping cross-validation showing the best performing single-proxy transfer functions for both DWT and pH were those based on bryophytes. The best performing transfer functions overall for DWT were those based on combined testate amoebae percentages, bryophytes and vascular plants; and, for pH, those based on testate amoebae and bryophytes. The comparison of DWT and pH inferred from testate amoeba percentages and presence/absence data showed similar general patterns but differences in the magnitude and timing of some shifts. These results show new directions for paleoenvironmental research, 1) suggesting that it is possible to build good-performing transfer functions using presence/absence data, although with some loss of accuracy, and 2) supporting the idea that multi-proxy inference models may improve paleoecological reconstruction. The performance of multi-proxy and single-proxy transfer functions should be further compared in paleoecological data.     

Tidal Freshwater Wetlands: Variation and Changes

Tidal freshwater wetlands (TFW) are situated in the upper estuary in a zone bordered upstream by the nontidal river and downstream by the oligohaline region. Here, discharge of freshwater from the river and the daily tidal pulse from the sea combine to create conditions where TFW develop. TFW are often located where human population density is high, which has led to wetland degradation or destruction. Globally, TFW are largely restricted to the temperate zone where the magnitude of annual river discharge prevents saline waters from penetrating too far inland. The constant input of river water delivers high loads of sediments, dissolved nutrients, and other suspended matter leading to high sedimentation rates and high nutrient levels. Prominent biogeochemical processes include the transformation of nitrogen by bacteria and immobilization of phosphate. A diverse, characteristic vegetation community develops which supports a rich fauna. Biotic diversity is highest in the high marsh areas and decreases in the lower levels where tidal inundation is greatest. Benthic fauna is rather poor in diversity but high in biomass compared to other regions of the estuary. Global climate change is a threat for this system directly by sea level rise, which will cause brackish water to intrude into the fresh system, and indirectly during droughts, which reduce river discharge. Salinity will affect the presence of flora and fauna and facilitates sulfate reduction of organic matter in the soil. Increased decomposition of organic matter following saltwater intrusion can result in a lowering of wetland surface elevation. The papers assembled in this issue focus on how these tidal freshwater wetlands have changed over recent time and how they may respond to new impacts in the future.     

Ammonium and phosphate dynamics in a Virginia salt marsh

Experimental chambers were used in a Virginia salt marsh to partition the tidal flux of dissolved nutrients occurring at the marsh surface and in the water column. On five dates from June to October 1989, six replicate chambers in the short Spartina alterniflora zone were monitored over complete tidal cycles. When reservoir water, used to simulate tidal flooding in the chambers, was initially low in dissolved nutrients, the marsh surface was a source of both ammonium and phosphate to the water column. Calculations of the physical processes of diffusion and advection could not account for total nutrient release from the marsh surface. We hypothesize the primary source of nutrients was organic matter mineralization in surface sediments, which released nutrients into the flooding water column. Assimilation (uptake) of phosphate measured in water-column incubation experiments was nearly equal to phosphate released from the marsh surface. Surface release of ammonium, however, was somewhat greater than water-column uptake. In this salt marsh, benthic production and release of ammonium and phosphate is comparable in magnitude to pelagic consumption, thereby yielding only a small “net” transfer of these nutrients to the estuary.     

Effects of Tidal Flooding on Juvenile Willows

Tidal wetlands are affected by sea level rise. In the tidal freshwater stretches of estuaries in the temperate zone, willows (Salix spp.) form tidal freshwater forests above the mean high water level. Willows tolerance to prolonged periodic flooding in riverine systems is well documented, whereas effects of tidal flooding on willows are largely unknown. Flooding stress may play a major role in regeneration failure of willows in tidal forest stands along estuarine shores, and juvenile willows might be specifically affected by partial or total submergence. To assess the tolerance of juvenile willows to tidal flooding, we conducted a mesocosm experiment with cuttings from Salix alba and Salix viminalis, which are both characteristic species for tidal freshwater forests in Europe. Cuttings originating from either fresh or brackish tidal forest stands were grown under four tidal treatments with up to a tidal flooding of 60 cm. A general tolerance to a tidal flooding of 60 cm was observed in chlorophyll fluorescence, growth rates, and biomass production in both willow species. Overall, S. alba showed higher leaf and shoot growth, whereas S. viminalis produced more biomass. S. alba with brackish origin performed worst with increasing tidal flooding, suggesting a possible pre-weakening due to stressful site conditions in tidal wetlands at the estuarine brackish stretch. This study demonstrates that juvenile willows of S. alba and S. viminalis tolerate tidal flooding of up to 60 cm. It is concluded that tidal inundation acts as a stress by causing submergence and soil anaerobiosis, but may also act as a subsidy by reestablishing aerobic conditions and thus maintaining willows performance. Therefore, we suggest investigations on Salix tidal flooding tolerance and possible effects of willows on tidal wetland accretion under estuarine field conditions.