How do non-halophyte locust trees thrive in temperate coastal regions: A study of salinity and multiple environmental factors on water uptake patterns

Understanding plant water use patterns is crucial for comprehending the dynamics of the soil–plant-atmosphere continuum and evaluating the adaptability of plants across diverse ecosystems. However, there remains a gap in our comprehension of non-halophyte plants' water uptake patterns and driving factors in temperate coastal regions. For this reason, we used locust trees (a widely planted non-halophyte tree species in northern China) as a study subject. We collected water isotope data (δ²H and δ¹⁸O) for locust trees xylem and soil over two consecutive growing seasons. The MixSIAR model was used along with five distinct sets of input data (single isotopes, uncorrected dual isotopes, and corrected dual isotopes incorporating δ²H data obtained by soil water line or cryogenic vacuum distillation methods) to infer water utilization patterns. The results indicated that locust trees primarily absorb shallow soil water (0–20 cm, 29.4% ± 16.9%) and deep soil water (120–180 cm, 24.7% ± 5.8%). Pearson's correlation analysis revealed the key driving factors behind water uptake patterns were vegetation transpiration and soil salinity. Remarkably, the build up of salts in the lower soil layer (60–120 cm) hinders the absorption of water by plants. To prevent high salt concentrations from affecting water uptake in non-halophyte plants, we recommend implementing sufficient irrigation from March to April each year to meet the water needs of plant growth and regulate the accumulation of salts in various soil layers. This study reveals the dynamic water utilization strategy of non-halophyte plants in temperate coastal regions, offering valuable information for water resources management.     

Water uptake of riparian plants in the lower Lhasa River Basin,South Tibetan Plateau using stable water isotopes

Riparian plants can adapt their water uptake strategies based on climatic and hydrological conditions within a river basin. The response of cold-alpine riparian trees to changes in water availability is poorly understood. The Lhasa River is a representative cold-alpine river in South Tibet and an under-studied environment. Therefore, a 96 km section of the lower Lhasa River was selected for a study on the water-use patterns of riparian plants. Plant water, soil water, groundwater and river water were measured at three sites for δ¹⁸O and δ²H values during the warm-wet and cold-dry periods in 2018. Soil profiles differed in isotope values between seasons and with the distance along the river. During the cold-dry period, the upper parts of the soil profiles were significantly affected by evaporation. During the warm-wet period, the soil profile was influenced by precipitation infiltration in the upper reaches of the study area and by various water sources in the lower reaches. Calculations using the IsoSource model indicated that the mature salix and birch trees (Salix cheilophila Schneid. and Betula platyphylla Suk.) accessed water from multiple sources during the cold-dry period, whereas they sourced more than 70% of their requirement from the upper 60–80 cm of the soil profile during the warm-wet period. The model indicated that the immature rose willow tree (Tamarix ramosissima Ledeb) accessed 66% of its water from the surface soil during the cold-dry period, but used the deeper layers during the warm-wet period. The plant type was not the dominant factor driving water uptake patterns in mature plants. Our findings can contribute to strategies for the sustainable development of cold-alpine riparian ecosystems. It is recommended that reducing plantation density and collocating plants with different rooting depths would be conducive to optimal plant growth in this environment.     

Seasonal changes in the water use strategies of three co‐occurring desert shrubs

Water is a major limiting factor in desert ecosystems. In order to learn how plants cope with changes in water resources over time and space, it is important to understand plant–water relations in desert region. Using the oxygen isotopic tracing method, our study clarified the seasonal changes in the water use strategies of three co‐occurring desert shrubs. During the 2012 growing season, δ¹⁸O values were measured for xylem sap, the soil water in different soil layers between 0 and 300 cm depth and groundwater. Based on the similarities in δ¹⁸O values for the soil water in each layer, three potential water sources were identified: shallow soil water, middle soil water and deep soil water. Then we calculated the percentage utilization of potential water sources by each species in each season using the linear mixing model. The results showed that the δ¹⁸O values of the three species showed a clear seasonal pattern. Reaumuria songarica used shallow soil water when shallow layer was relatively wet in spring, but mostly took up middle soil water in summer and autumn. Nitraria tangutorum mainly utilized shallow and middle soil water in spring, but mostly absorbed deep soil water in summer and autumn. Tamarix ramosissima utilized the three water sources evenly in spring and primarily relied on deep soil water in summer and autumn. R. songarica and N. tangutorum responded quickly to large rainfall pulses during droughts. Differential root systems of the three species resulted in different seasonal water use strategies when the three competed for water. Copyright © 2013 John Wiley & Sons, Ltd.     

Plant source water apportionment using stable isotopes: A comparison of simple linear,two‐compartment mixing model approaches

Plant source water identification using stable isotopes is now a common practice in ecohydrological process investigations. Notwithstanding, little critical evaluation of the approaches for source apportionment have been conducted. Here, we present a critical evaluation of the main methods used for source apportionment between vadose and saturated zone water: simple mass balance and Bayesian mixing models. We leverage new isotope stem water samples from a diverse set of tree species in a strikingly uniform terrain and soil conditions at the Christchurch Botanic Garden, New Zealand. Our results show that using δ²H alone in a simple, two‐source mass balance approach leads to erroneous results, particularly an apparent overestimation of groundwater contribution to xylem. Alternatively, using both δ²H and δ¹⁸O in a Bayesian inference framework improves the source water estimates and is more useful than the simple mass balance approach, particularly when soil and groundwater contributions are relatively disproportionate. We suggest that plant source water quantification methods should take into consideration the possible effects of ²H/¹H fractionation. The Bayesian inference approach used here may be less sensitive to ²H/¹H fractionation effects than simple mass balance methods.     

Water uptake by saltcedar (Tamarix ramosissima) in a desert riparian forest: responses to intra‐annual water table fluctuation

There is considerable interest in naturalizing flow regime on managed rivers to slow the spread of saltcedar (Tamarix ramosissima) invasion in southwestern USA or to preserve riparian forests dominated by saltcedar and other species in northwestern China. However, little is known about the responses of established saltcedar in water sources to frequent intra‐annual fluctuation of water table resulting from this new, more dynamic flow regime. This study investigates how saltcedar at a riparian site in the middle reaches of the Heihe River, northwest China, responds in water sources use to intra‐annual water table fluctuations. Stable oxygen isotope was employed to determine accurate depth at which saltcedar obtains its water supply, and soil moisture monitoring was used to determine sources of plant‐available soil water. We found that the primary zone of water uptake by saltcedar were stable at 25–60 cm depth, but the water sources used by saltcedar switched between groundwater and soil moisture with the water table fluctuations. Saltcedar derived its water from groundwater when water table was at depth less than 60 cm but switched to soil moisture at 25–60 cm depth when water table declined. It is supposed that the well‐developed clay layer at 60–80 cm depth constrained lateral roots of saltcedar to the soil layers above 60 cm, while the fine‐textured soils at this site, which were periodically resaturated by rising groundwater before the stored soil moisture had become depleted, provided an important water reservoir for saltcedar when groundwater dropped below the primary zone of fine roots. The root distribution of saltcedar may also be related to local groundwater history. The quick decline in water table in the early 1980s when the riparian saltcedar had established may strand its roots in the shallow unsaturated zone. We suggested that raising the water table periodically instead of maintaining it invariably above the rooting depth could sustain desired facultative phreatophytes while maximizing water deliveries. Copyright © 2015 John Wiley & Sons, Ltd.     

Spatio‐temporal heterogeneity in soil water stable isotopic composition and its ecohydrologic implications in semiarid ecosystems

Spatio‐temporal heterogeneity in soil water content is recognized as a common phenomenon, but heterogeneity in the hydrogen and oxygen isotope composition of soil water, which can reveal processes of water cycling within soils, has not been well studied. New advances are being driven by measurement approaches allowing sampling with high density in both space and time. Using in situ soil water vapour probe techniques, combined with conventional soil and plant water vacuum distillation extraction, we monitored the hydrogen and oxygen stable isotopic composition of soil and plant waters at paired sites dominated by grasses and Gambel's oak (Quercus gambelii) within a semiarid montane ecosystem over the course of a growing season. We found that sites spaced only 20 m apart had profoundly different soil water isotopic and volumetric conditions. We document patterns of depth‐ and time‐explicit variation in soil water isotopic conditions at these sites and consider mechanisms for the observed heterogeneity. We found that soil water content and isotopic variability were damped under Q. gambelii, perhaps due in part to hydraulic redistribution of deep soil water or groundwater by Q. gambelii in these soils relative to the grass‐dominated site. We also found some support for H isotope discrimination effects during water uptake by Q. gambelii. In this ecosystem, the soil water content was higher than that at the neighbouring Grass site, and thus, 25% more water was available for transpiration by Q. gambelii compared with the Grass site. This work highlights the role of plants in governing soil water variation and demonstrates that they can also strongly influence the isotope ratios of soil water. The resulting fine‐scale heterogeneity has implications for the use of isotope tracers to study soil hydrology and evaporation and transpiration fluxes to improve understanding of water cycling through the soil–plant–atmosphere continuum.     

Water uptake by trees in a riparian hardwood forest (Rhine floodplain,France)

Water flow in the soil–root–stem system was studied in a flooded riparian hardwood forest in the upper Rhine floodplain. The study was undertaken to identify the vertical distribution of water uptake by trees in a system where the groundwater is at a depth of less than 1 m. The three dominant ligneous species (Quercus robur, Fraxinus excelsior and Populus alba) were investigated for root structure (vertical extension of root systems), leaf and soil water potential (Ψ_m), isotopic signal (¹⁸O) of soil water and xylem sap. The root density of oak and poplar was maximal at a depth of 20 to 60 cm, whereas the roots of the ash explored the surface horizon between 0 and 30 cm, which suggests a complementary tree root distribution in the hardwood forest. The flow density of oak and poplar was much lower than that of the ash. However, in the three cases the depth of soil explored by the roots reached 1·2 m, i.e. just above a bed of gravel. The oak roots had a large lateral distribution up to a distance of 15 m from the trunk. The water potential of the soil measured at 1 m from the trunk showed a zone of strong water potential between 20 and 60 cm deep. The vertical profile of soil water content varied from 0·40 to 0·50 cm³ cm^?3 close to the water table, and 0·20 to 0·30 cm³ cm^?3 in the rooting zone. The isotopic signal of stem water was constant over the whole 24‐h cycle, which suggested that the uptake of water by trees occurred at a relatively constant depth. By comparing the isotopic composition of water between soil and plant, it was concluded that the water uptake occurred at a depth of 20 to 60 cm, which was in good agreement with the root and soil water potential distributions. The riparian forest therefore did not take water directly from the water table but from the unsaturated zone through the effect of capillarity. Copyright © 2007 John Wiley & Sons, Ltd.     

Effects of juniper removal and rainfall variation on tree transpiration in a semi‐arid karst: evidence of complex water storage dynamics

Brush removal is widely practiced as a tool for increasing groundwater recharge, but its efficacy depends greatly on the way in which the removed species interact with the hydrological system relative to the vegetation replacing it. We examined the effects of Ashe juniper removal in the recharge zone of the Edwards Aquifer, Texas, USA, a karst aquifer. The study was conducted in an Ashe juniper (Juniperus ashei)–live oak (Quercus fusiformis) woodland on a hill slope composed of rocky, shallow soils over fractured limestone bedrock. Ashe juniper is a native species that has been encroaching grasslands and savannas over the past century. In September 2008, a plot was cleared of 90% of its juniper trees. Tree transpiration, predawn water potentials and vegetation cover across the cleared plot and an adjacent reference site were measured from May 2009 to December 2011. Stand‐level tree transpiration from May 2009 to March 2010 was diminished by a severe summer drought in 2009, from which trees were slow to recover. Subsequently, tree transpiration was 5–10× higher in the woodland compared to the clearing. For all of 2011, also a drought year, tree transpiration in the woodland exceeded precipitation inputs, indicating a high capacity for water storage at the study site. However, site differences for oak trees were generally larger than for juniper trees. While juniper removal accounted for a 431 mm year^?1 difference in tree transpiration between sites, vegetation cover in the clearing increased from 42% to 90% over two years, suggesting that understory growth was increasingly compensating for the loss of juniper transpiration. We conclude that the removal of a relatively shallow‐rooted tree, when replaced with herbaceous vegetation and low shrubs, has little effect on deep recharge. By contrast, successive years of precipitation extremes may be more effective increasing recharge by lowering the water transport capacity of trees in the aftermath of severe drought. Copyright © 2016 John Wiley & Sons, Ltd.     

Water use strategies of two co‐occurring tree species in a semi‐arid karst environment

The flow of precipitation from the surface through to groundwater in karst systems is a complex process involving storage in the unsaturated zone and diffuse and preferential recharge pathways. The processes associated with this behaviour are not well understood, despite the prevalence of karst aquifers being used as freshwater supplies. As a result, uncertainty regarding the ecohydrological processes in this geological setting remains large. In response to the need to better understand the impact of woody vegetation on groundwater recharge, annual evapotranspiration (ET) rates and tree water sources were measured for two years above a shallow, fresh karst aquifer. Water use strategies of the co‐occurring Eucalyptus diversifolia subsp. diversifolia Bonpl. and Allocasuarina verticillata (Lam.) L. Johnson were investigated using a monthly water balance approach, in conjunction with measurement of the stable isotopes of water, leaf water potentials and soil matric potentials. The results suggest that it is unlikely groundwater resources are required to sustain tree transpiration, despite its shallow proximity to the soil surface, and that similarities exist between ET losses and the estimated long‐term average rainfall for this area. Irrespective of stand and morphological differences, E. diversifolia and A. verticillata ET rates showed remarkable convergence, demonstrating the ability of these co‐occurring species to maximise their use of the available precipitation, which avoids the requirement to differentiate between these species when estimating ET at a landscape scale. We conclude that the water holding capacity of porous geological substrates, such as those associated with karst systems, will play an important role in equilibrating annual rainfall variability and should be considered when assessing ecohydrological links associated with karst systems. Copyright © 2013 John Wiley & Sons, Ltd.     

Evidence for variations in cryogenic extraction deuterium biases of plant xylem water across foundational northeastern US trees

Measurements of oxygen and hydrogen isotopes in plant xylem water (²H, ¹⁸O) have helped to redefine conceptual and numerical models of the hydrological cycle and understand how plants compete for subsurface water. Recent experiments have shown that Cryogenic Vacuum Extraction (CVE) of plant xylem water can result in a δ²H bias. We tested if CVE δ²H-biases varied significantly across seven foundational northeastern US forest trees with a series of tree core rehydration experiments. Our analysis demonstrated that CVE δ²H-biases were well predicted by sample gravimetric water content and varied significantly with tree species identity. We show that species-level δ²H-bias corrections can result in substantially different understandings of plant water uptake and transpiration versus uncorrected data or generic bias corrections. This research demonstrates an urgent need for the critical evaluation of CVE for plant water extraction. In the absence of a stronger understanding of CVE δ²H-biases, we recommend that xylem water δ²H observations should not be used in plant water uptake studies.     

Examination and parameterization of the root water uptake model from stem water potential and sap flow measurements

A simple field‐based method for directly parameterizing root water uptake models is proposed. Stem psychrometers and sap flow meters are used to measure stem water potential and plant transpiration rate continuously and simultaneously. Predawn stem water potential is selected as a surrogate for root zone soil water potential to examine and parameterize the root water uptake–water stress response functions. The method is applied to two drooping sheoak (Allocasuarina verticillata) trees for a period of 80 days, covering both a dry season and a wet season. The results indicate that the S‐shape function is more appropriate than the Feddes piecewise linear function for drooping sheoak to represent the effect of soil moisture stress on its root water uptake performance. Besides, the water stress function is found to be not only a function of soil moisture but also dependent of the atmospheric demand. As a result, the water stress function is corrected for the effect of atmospheric conditions. Copyright © 2012 John Wiley & Sons, Ltd.     

Sap flow and plant water sources for typical vegetation in a subtropical humid karst area of southwest China

Plant water use plays a crucial part in the soil–plant-atmosphere continuum. However, in karst regions, plants frequently suffer from water shortages due to low soil water storage capacity. Therefore, it is necessary to understand plant water consumption (as determined by sap flow) and seasonal variation of water sources to improve water management in karst catchments. In this study, thermal dissipation probes (TDP), calibrated using empirical equations, were used to measure the sap flow of three typical woody vegetations, including Coriaria nepalensis (sparse-shrub), Toona sinensis (secondary forest) and Populus adenopoda (shrub-grass). Oxygen and hydrogen stable isotopes were used to analyze seasonal variation of plant water sources. The results showed that: (1) T. Sinensis (3.89 ± 3.87 L·day⁻¹) had significantly higher daily sap flow than C. nepalensis (0.33 ± 0.37 L·day⁻¹) and P. adenopoda (0.09 ± 0.12 L·day⁻¹); (2) daily sap flow was closely correlated to photosynthetically active radiation (PAR) and vapour pressure deficit (VPD); (3) over the entire study period, plants mainly used water from the surface soil horizons; and (4) a greater proportion of epikarst water was used for C. nepalensis than by T. sinensis and P. adenopoda over the whole growth stage, and more epikarst water was used in early and mid-growth stages compared to the late stage for the three species. This study contributes to a deeper understanding of the plant water use strategies in karst regions, and is helpful for ecosystem management.     

Using stable isotopes to quantify water sources for trees and shrubs in a riparian cottonwood ecosystem in flood and drought years

Riparian cottonwood forests in dry regions of western North America do not typically receive sufficient growing season precipitation to completely support their relatively high transpiration requirements. Water used in transpiration by riparian ecosystems must include alluvial groundwater or water stored in the potentially large reservoir of the unsaturated soil zone. We used the stable oxygen and hydrogen isotope composition of stem xylem water to evaluate water sources used by the dominant riparian cottonwood (Populus spp.) trees and shrubs (Shepherdia argentea and Symphoricarpos occidentalis) in Lethbridge, Alberta, during 3 years of contrasting environmental conditions. Cottonwoods did not exclusively take up alluvial groundwater but made extensive use of water sourced from the unsaturated soil zone. The oxygen and hydrogen isotope compositions of cottonwood stem water did not strongly overlap with those of alluvial groundwater, which were closely associated with the local meteoric water line. Instead, cottonwood stem water δ¹⁸O and δ²H values were located below the local meteoric water line, forming a line with a low slope that was indicative of water exposed to evaporative enrichment of heavy isotopes. In addition, cottonwood xylem water isotope compositions had negative values of deuterium excess (d‐excess) and line‐conditioned (deuterium) excess (lc‐excess), both of which provided evidence that water taken up by the cottonwoods had been exposed to fractionation during evaporation. The shrub species had lower values of d‐excess and lc‐excess than had the cottonwood trees due to shallower rooting depths, and the d‐excess values declined during the growing season, as shallow soil water that was taken up by the plants was exposed to increasing, cumulative evaporative enrichment. The apparent differences in functional rooting pattern between cottonwoods and the shrub species, strongly influenced the ratio of net photosynthesis to stomatal conductance (intrinsic water‐use efficiency), as shown by variation among species in the δ¹³C values of leaf tissue.     

Soil water movement and nutrient cycling in semi-arid rangeland: vegetation change and system resilience

Recent decades have seen rapid intensification of cattle production in semi-arid savannah ecosystems, increasingly on formalized ranch blocks. As a result, vegetation community changes have occurred, notably bush encroachment (increased bush dominance) in intensively grazed areas. The exact causes of this vegetation change remain widely debated. Previous studies have suggested: (i) increased leaching of water and nutrients into the subsoil in intensively grazed areas provides deeper rooting bush species with a competitive advantage for soil water and nutrients, and (ii) nutrient leaching may be exacerbated by nutrient inputs from cattle dung and urine. Our research in the Eastern Kalahari showed that in infertile sandy soils both the magnitude of soil water and concentration of soil nutrients leached into the subsoil is largely unaffected by the ecological and biochemical effects of increased cattle use. We found that despite the high soil hydraulic conductivity ( &greaterno;12 cm h⁻¹), relatively high subsoil moisture contents and the restriction of water movement to matrix flow pathways prevent leaching losses beyond the rooting zone of savannah grass species. No significant differences in patterns of soil water redistribution were noted between bush dominant and grass dominant sites. We also found that the low nutrient status of Kalahari soils and leachate movement as matrix flow combine to allow nutrient adsorption on to soil particles. Nutrient adsorption ensures that nitrogen and phosphorus cycling remains topsoil dominated even following the removal of vegetation and direct nutrient inputs in cattle dung and urine. This conclusion refutes environmental change models that portray increases in the leaching of soil water and available nitrogen as a major factor causing bush encroachment. This provides a possible explanation for the now widely cited, but hitherto unexplained, resilience of dryland soils. We suggest that infertile sandy soils appear resilient to changes in soil water distribution and nutrient availability caused by increased cattle use. Hence, soil characteristics contribute to the resilience to permanent ecological change that is increasingly recognized as an attribute of semi-arid rangelands. © 1998 John Wiley & Sons, Ltd.     

Tree water deficit and dynamic source water partitioning

The stable isotopes of hydrogen and oxygen (δ²H and δ¹⁸O, respectively) have been widely used to investigate tree water source partitioning. These tracers have shed new light on patterns of tree water use in time and space. However, there are several limiting factors to this methodology (e.g., the difficult assessment of isotope fractionation in trees, and the labor-intensity associated with the collection of significant sample sizes) and the use of isotopes alone has not been enough to provide a mechanistic understanding of source water partitioning. Here, we combine isotope data in xylem and soil water with measurements of tree's physiological information including tree water deficit (TWD), fine root distribution, and soil matric potential, to investigate the mechanism driving tree water source partitioning. We used a 2 m³ lysimeter with willow trees (Salix viminalis) planted within, to conduct a high spatial–temporal resolution experiment. TWD provided an integrated response of plant water status to water supply and demand. The combined isotopic and TWD measurement showed that short-term variation (within days) in source water partitioning is determined mainly by plant hydraulic response to changes in soil matric potential. We observed changes in the relationship between soil matric potential and TWD that are matched by shifts in source water partitioning. Our results show that tree water use is a dynamic process on the time scale of days. These findings demonstrate tree's plasticity to water supply over days can be identified with high-resolution measurements of plant water status. Our results further support that root distribution alone is not an indicator of water uptake dynamics. Overall, we show that combining physiological measurements with traditional isotope tracing can reveal mechanistic insights into plant responses to changing environmental conditions.     

How important is groundwater availability and stream perenniality to riparian and floodplain tree growth?

Riparian vegetation is important for stream functioning and as a major landscape feature. For many riparian plants, shallow groundwater is an important source of water, particularly in areas where rainfall is low, either annually or seasonally, and when extended dry conditions prevail for all or part of the year. The nature of tree water relationships is highly complex. Therefore, we used multiple lines of evidence to determine the water sources used by the dominant tree species Eucalyptus camaldulensis (river red gum), growing in riparian and floodplain areas with varying depth to groundwater and stream perenniality. Dendrometer bands were used to measure diel, seasonal, and annual patterns of tree water use and growth. Water stable isotopes (δ²H and δ¹⁸O) in plant xylem, soil water, and groundwater were measured to determine spatial and temporal patterns in plant water source use. Our results indicated riparian trees located on relatively shallow groundwater had greater growth rates, larger diel responses in stem diameter, and were less reactive to extended dry periods, than trees in areas of deep groundwater. These results were supported by isotope analysis that suggested all trees used groundwater when soil water stores were depleted at the end of the dry season, and this was most pronounced for trees with shallow groundwater. Trees may experience more frequent periods of water deficit stress and undergo reduced productivity in scenarios where water table accessibility is reduced, such as drawdown from groundwater pumping activities or periods of reduced rainfall recharge. The ability of trees to adapt to changing groundwater conditions may depend on the speed of change, the local hydrologic and soil conditions as well as the species involved. Our results suggest that E. camaldulesis growing at our study site is capable of utilizing groundwater even to depths >10 m, and stream perenniality is likely to be a useful indicator of riparian tree use of groundwater.     

Carbon,nitrogen, and water stable isotopes in plant tissue and soils across a moisture gradient in Puerto Rico

Stable isotopes in the water molecule (²H or D and ¹⁸O), carbon, and nitrogen are useful tracers and integrators of processes in plant ecohydrological systems across scales. Over the last few years, there has been growing interest in regional to continental scale synthesis of stable isotope data with a view to elucidating biogeochemical and ecohydrological patterns. Published datasets from the humid tropics, however, are limited. To be able to contribute to bridging the “data gap” in the humid tropics, here, we publish a relatively novel and unique suite of δ¹³C, δ¹⁵N, δ²H, and δ¹⁸O isotope data from three sites across a moisture gradient and contrasting land use in Puerto Rico. Plant tissue (xylem and leaf) samples from two species of mahogany (Swietenia macrophylla and Swietenia mahagoni) and soil samples down to 60 cm in the soil profile were collected in relatively “wet” (July 2012) and “dry” (February 2013) periods at two sites in northeastern (Luquillo) and southwestern (Susua) Puerto Rico. The same sampling suite is also being made available from a highly urbanized site in the capital San Juan. Leaf samples taken in July 2012 and February 2013 were analyzed for δ¹³C and δ¹⁵N; all xylem and bulk soil samples were analyzed for δ²H and δ¹⁸O. Soil samples taken in July 2012 were analyzed for δ¹³C and δ¹⁵N. Leaf δ¹⁵N and δ¹³C dataset showed patterns that are possibly associated with site differences. While spatial patterns were also apparent in soil δ¹⁵N and δ¹³C dataset, the positively linear δ¹⁵N –δ¹³C relationship tends to weaken with site moisture. Soil depth and site moisture patterns were also observed in the δ²H and δ¹⁸O datasets of bulk soil and xylem samples. The purpose of these datasets is to provide baseline information on soil–plant water (δ²H and δ¹⁸O, N = 319), δ¹³C (N = 272), and δ¹⁵N (N = 269) that may be useful in a wide range of research questions from ecohydrological relations to biogeochemical patterns in soils and vegetation.     

Investigation of soil water hydrological process in the permafrost active layer using stable isotopes

Here, we studied the isotope characteristics and source contributions of soil water in the permafrost active layer by collecting soil samples in July 2018 in Yangtze River basin. Soil moisture and temperature showed decreasing trends from 0–80 cm, and an increasing trend from 80–100 cm. The value of δ¹⁸O and δD first increased and then decreased in the soil profile of 0–100 cm; however, d-excess increased from 0–100 cm. δ¹⁸O values became gradually positive from the southwest to northeast of the study area, while d-excess gradually increased from southeast to northwest. The evaporation water line (EL) was δD = 7.56 δ¹⁸O + 1.50 (R² = 0.90, p < 0.01, n = 96). Due to intense solar radiation and evaporation on the Tibetan Plateau, the elevation did not impact the surface soil. The altitude effect of the soil depths of 0–20 cm was not obvious, but the other soil layers had a significant altitude effect. Soil moisture and temperature were closely related to the stable isotopic composition of soil water. The contribution of precipitation to soil water on the sunny slope was 86%, while the contribution of the shady slope was 84%. However, the contribution of ground ice to soil water on sunny slope was 14% and the shady slope was 16%. The contribution of ground ice to soil water increased with increasing altitude on the sunny slope, but the contribution of ground ice to soil water had no obvious trend on the shady slope.     

Amending Soils with Hydrogels Increases the Biomass of Nine Tree Species under Non‐water Stress Conditions

The classical aim of the application of super absorbent polyacrylate (SAPs) hydrogels is the prolonging of plant survival under water stress. Their effect on plant growth during non‐water stress conditions is not known. This study examined the root and shoot biomass of seedlings of nine tree species; Eucalyptus grandis, Eucalyptus citriodora, Pinus caribaea, Araucaria cunninghamii, Melia volkensii, Grevillea robusta, Azadirachta indica, Maesopsis eminii and Terminalia superba. The seedlings were potted in five soil types; sand, sandy loam, loam, silt loam and clay. These were amended at two hydrogel levels: 0.2 and 0.4% w/w and grown under controlled conditions in a green house. Root and shoot growth responses of the seedlings were determined by measuring the dry weight of the roots, stems, leaves and twigs. The addition of either 0.2 or 0.4% hydrogel to the five soil types resulted in a significant increase of the root dry weight (p < 0.001) in eight tree species compared to the controls after 8 wk of routine watering. Also, the dry weight of stems and leaves and twigs were significantly (p < 0.001) higher in the nine tree species potted in hydrogel amended soil types than in the hydrogel free controls. These results suggested that hydrogel amendment enhances the efficiency of water uptake and utilization of photosynthates of plants grown in soils which have water contents close to field capacity.     

Hydraulic redistribution by deeply rooted grasses and its ecohydrologic implications in the southern Great Plains of North America

Perennial bioenergy crops with deep (>1 m) rooting systems, such as switchgrass (Panicum virgatum L.), are hypothesized to increase carbon storage in deep soil. Deeply rooted plants may also affect soil hydrology by accessing deep soil water for transpiration, which can affect soil water content and infiltration in deep soil layers, thereby affecting groundwater recharge. Using stable H and O isotope (δ²H and δ¹⁸O) and ³H values, we studied the soil water conditions at 20–30 cm intervals to depths of 2.4–3.6 m in paired fields of switchgrass and shallow rooted crops at three sites in the southern Great Plains of North America. We found that soil under switchgrass had consistently higher soil water content than nearby soil under shallow-rooted annual crops by a margin of 15%–100%. Soil water content and isotopic depth profiles indicated that hydraulic redistribution of deep soil water by switchgrass roots explained these observed soil water differences. To our knowledge, these are the first observations of hydraulic redistribution in deeply rooted grasses, and complement earlier observations of dynamic soil water fluxes under shallow-rooted grasses. Hydraulic redistribution by switchgrass may be a strategy for drought avoidance, wherein the plant may actively prevent water limitation. This raises the possibility that deeply rooted grasses may be used to passively subsidize soil water to more shallow-rooted species in inter-cropping arrangements.