用生物模拟采样技术模拟研究不同取代氯酚在金鱼(Crassius auratus)体内的富集和降解

在实验室控制条件的流动体系中,研究了九种典型氯酚类化合物在半渗透膜生物模拟采样器件（SPMD）和金鱼体内的富集动力学过程。基于氯酚污染物在SPMD中的生物浓缩和在鱼体脂肪中生物富集的差别,研究了氯酚污染物在生物体内的降解性和降解作用的结构／效应关系。结果表明氯酚类污染物在SPMD中的浓缩作用和在鱼体中的富集作用非常相似。氯酚化合物的生物降解性随氯取代基数量增加而降低,并与氯取代基的位置有关。     

The effects of adsorptive materials on microbial community composition and PAH degradation at the sediment capewater interface

Capping with layers of inert or adsorptive materials is used to control the release of polycyclic aromatic hydrocarbons(PAH)in sediment but little is known about microbial degradation processes in these materials.A rich native microbial community inhabits the sediment bed,and capping media can influence enrichment and biodegradation activity of benthic microorganisms.The aim of this study was to evaluate the effects of capping media(sand,organoclay,and powdered activated carbon[PAC])on microbial communities under oxic conditions typical of the capewater interface,where naphthalene degradation(model PAH)is likely to be maximized.Bench scale experiments compared naphthalene concentrations,nahAc biomarker abundance,microbial community composition,and cellular attachment in systems amended with adsorptive and non-adsorptive capping materials.Results indicate that activated carbon promoted and enhanced bioactivity;PAC treatments showed high biodegradation rates,nahAc biomarker levels,and attached biological growth consistent with enrichment of the PAHdegrading genus Pseudomonae.In contrast,sand did not enhance biological activity compared to media-free systems.Naphthalene strongly influenced microbial community composition at the species level in all treatments except organoclay,which promoted biological signatures commonly associated with impeding degradation activity.Data overall suggest that adsorptive capping materials can both promote(PAC)and inhibit(organoclay)bioactivity in the surficial layer of caps,indicating that media selection is critical to the design of bioactive capping systems.     

Biodegradation and transport of benzene,toluene, and xylenes in a simulated aquifer: comparison of modelled and experimental results

Both laboratory experiments and numerical modelling were conducted to study the biodegradation and transport of benzene–toluene–xylenes (BTX) in a simulated semi‐confined aquifer. The factors incorporated into the numerical model include advection, hydrodynamic dispersion, adsorption, and biodegradation. The various physico‐chemical parameters required by the numerical model were measured experimentally. In the experimental portion of the study, BTX compounds were introduced into the aquifer sand. After the contaminants had been transported through the system, BTX concentrations were measured at 12 equally spaced wells. Subsequently, microorganisms obtained from the activated sludge of a sewage treatment plant and cultured in BTX mixtures were introduced into the aquifer through the 12 sampling wells. The distribution data for BTX adsorption by the aquifer sand form a nonlinear isotherm. The degree of adsorption by the sand varies, depending on the composition of the solute. The degradation time, measured from the time since the bacteria were added to the aquifer until a specific contaminant was no longer detectable, was 35–42 h for BTX. The dissolved oxygen, after degradation by BTX compounds and bacteria, was consumed by about 40–60% in the entire simulated aquifer; thus the aerobic conditions were maintained. This study provides insights for the biodegradation and transport of BTX in aquifers by numerical modelling and laboratory experiments. Experimental and numerical comparisons indicate that the results by Monod degradation kinetics are more accurate than those by the first‐order degradation kinetics. Copyright © 2002 John Wiley & Sons, Ltd.     

Simultaneous Determination of 11 Sulfonamides by HPLC–UV and Application for Fast Screening of Their Aerobic Elimination and Biodegradation in a Simple Test

Sulfonamides (SAs) are one of the most frequently used antibiotics. SAs have been found in various environmental compartments. If SAs are not degraded in the environment, they can affect bacteria by their antibiotic properties and contribute to bacterial antibiotic resistance. Therefore, the biodegradability of 11 SAs (sulfanilamide, sulfaguanidine monohydrate, sulfadiazine, sulfathiazole, sulfapyridine, sulfamerazine, sulfamethoxypyridazine, sulfachloropyridazine, sulfamethazine, sulfamethoxazole, and sulfadimethoxine) was studied. For this purpose, the Closed Bottle Test (CBT, OECD 301D) was performed, which includes a toxicity control. In order to monitor the environmental fate of the parent compound and to check for transformation products, a simple, efficient, and reliable HPLC–UV method for the simultaneous determination of these SAs has been developed. Acetonitrile and water (with 0.1% formic acid) were used as mobile phase solvents for gradient elution. The method was validated in terms of precision, detection and quantitation limits, selectivity, and analytical solution stability. In the CBT, none of these SAs was readily biodegradable. The HPLC–UV analysis confirmed that no degradation of any SA took place. In the toxicity control, these SAs showed no toxic effect in the used concentration of environmental bacteria applied in the test.     

Geochemical significance of hydrocarbons desorbed from asphaltenes

Saturate hydrocarbon fractions desorbed (cleaned) from asphaltenes extracted and separated from two core samples (a tar sand and a reservoir sandstone sample) were analyzed by gas chromatography-mass spectrometry (GC-MS). The results indicated thatn-alkanes adsorbed in asphaltenes were kept quite well during and after severe biodegradation over 360 Ma. Hydrocarbon desorption could be an alternative method of, or even better than the pyrolysis of asphaltenes to reconstruct the hydrocarbon fingerprints of primary oils and extend the understanding of the origins of hydrocarbon reservoirs. Project supported by the Chinese Academy of Sciences, State Key Laboratory of Organic Geochemistry and Research Center of Tarim Petroleum Exploration and Development.     

表面活性剂在滇池水体中的生物降解

利用河水衰减法研究阴离子表面活性剂(LAS)和非离子表面活性剂(NIS)在滇池水体中不同条件下的生物降解规律.结果表明:在滇池自然水体中,LAS和NIS均能生物降解,在试验时间(30d)内,L,AS的降解率达95%以上,NIS的降解率达92%以上,其降解动力学遵从二级动力模型.改变水温、表面活性剂初始浓度、pH值以及添加营养物质(葡萄糖或磷酸氢二钠)均对LAS和NIS的降解有一定的影响.水温对表面活性剂生物降解影响最大,当水温从20℃增至30℃时,LAS的降解速率从0.73-1增至2.10d-1,NIS降解半衰期从11.5d减至4.4d;表面活性剂初始浓度增加,降解半衰期有所增加;表面活性剂在pH 7时的降解性能略优于pH 10时的降解性能;添加葡萄糖抑制LAS和NIS的降解,而添加磷源磷酸氢二钠对其降解有一定的促进作用;曝气能促进LAS的降解,但对NIS降解的促进作用并不明显.     

蓝藻膦酸酯代谢及其生态意义研究进展

膦酸酯是新近发现的一类生物可利用磷,其广泛存在于水生生态系统,是水体有机磷库的重要组成,构成水体磷氧化还原循环的关键环节。随着研究的深入,越来越多的蓝藻藻株被证实参与膦酸酯的生物利用与合成,有助于阐明其适应低磷环境的机制,促进了对蓝藻磷策略与水体磷循环的全面理解,具有重要的生态学意义。与此同时,蓝藻对膦酸酯的利用性状,使得膦酸酯作为环境污染物与蓝藻磷源的双重作用开始显现,这会对水体浮游生物竞争格局的研究产生深远影响;而蓝藻的甲基膦酸代谢也被认为是水体好氧甲烷发生的来源之一。本文回顾了蓝藻膦酸酯利用与合成的研究历史,对目前蓝藻膦酸酯代谢过程生态风险与生态意义的研究进展进行了梳理,并对这一领域未来发展趋势进行了评述。     

Numerical simulation of transport and sequential biodegradation of chlorinated aliphatic hydrocarbons using CHAIN_2D

Microbiological degradation of perchloroethylene (PCE) under anaerobic conditions follows a series of chain reactions, in which, sequentially, trichloroethylene (TCE), cis‐dichloroethylene (c‐DCE), vinylchloride (VC) and ethene are generated. First‐order degradation rate constants, partitioning coefficients and mass exchange rates for PCE, TCE, c‐DCE and VC were compiled from the literature. The parameters were used in a case study of pump‐and‐treat remediation of a PCE‐contaminated site near Tilburg, The Netherlands. Transport, non‐equilibrium sorption and biodegradation chain processes at the site were simulated using the CHAIN_2D code without further calibration. The modelled PCE compared reasonably well with observed PCE concentrations in the pumped water. We also performed a scenario analysis by applying several increased reductive dechlorination rates, reflecting different degradation conditions (e.g. addition of yeast extract and citrate). The scenario analysis predicted considerably higher concentrations of the degradation products as a result of enhanced reductive dechlorination of PCE. The predicted levels of the very toxic compound VC were now an order of magnitude above the maximum permissible concentration levels. Copyright © 1999 John Wiley & Sons, Ltd.     

A coupled transport and reaction model for long column experiments simulating bank filtration

Within the scope of the interdisciplinary Natural and Artificial Systems for Recharge and Infiltration research project dealing with riverbank filtration processes at the Berlin water works, a semi‐technical column experiment has been ongoing since January 2003. Here, a 30 m long soil column is infiltrated with surface water sampled from Lake Tegel (Berlin, Germany) under saturated flow conditions. Changes in pore water hydrochemistry sampled on 21 non‐equidistant distributed points are verified by coupled transport and reaction modelling. The objective of reactive transport modelling was to identify the main biogeochemical processes within the soil column during the flushing experiment as a conceptual model for riverbank filtration. Modelling was done with a combination of MATLAB and PHREEQC. The main processes identified are: (1) biogeochemical degradation due to interaction of natural surface water with the soil matrix; (2) continuous dissolution of refractory air bubbles from the soil column matrix. Copyright © 2006 John Wiley & Sons, Ltd.     

Formation mechanisms of heavy oils in the Liaohe Western Depression,Bohai Gulf Basin

The Liaohe Oilfield in the Liaohe Western Depression of the Bohai Gulf Basin is the third-largest oil producing province and the largest heavy oil producing oilfield in China. A total of 65 oil samples,35 rock samples and 36 reservoir sandstone samples were collected and analyzed utilizing conventional geochemical and biogeochemical approaches to unravel the mechanisms of the formation of the heavy oils. Investigation of the oils with the lowest maturity compared with the oils in the Gaosheng and Niuxintuo oilfields indicates no apparent relation between the maturity and physical properties of the heavy oils. It is suggested that the heavy oil with primary origin is not likely the main mechanism re-sponsible for the majority of the heavy oils in the Liaohe Western Slope. The absence and/or depletion of n-alkanes etc.,with relatively low molecular weight and the occurrence of 25-norhopane series in the heavy oils as well as the relatively high acidity of the oils all suggest that the majority of the heavy oils once experienced secondary alteration. The fingerprints of the total scanning fluorescence (TSF) of the inner adsorbed hydrocarbons on the reservoir grains and the included hydrocarbons in fluid inclusions are similar to that of the normal oils in the area but are different from the outer adsorbed and reser-voired free oils at present,further indicating that most of the heavy oils are secondary in origin. Analyses of bacteria (microbes) in 7 oil samples indicate that anaerobic and hyperthermophilic Ar-chaeoglobus sp. are the dominant microbes relevant to oil biodegradation,which coincides with the shallow commercial gas reservoirs containing anaerobic bacteria derived gas in the Gaosheng and Leijia teotonic belts. The biodegradation most likely occurs at the water/oil interface,where the forma-tion water is essential for microbe removal and nutrient transportation. We think that biodegradation,water washing and oxidization are interrelated and are the main mechanisms for the formation of the heavy oils. Biodegradation was the predominant process with water washing being a prerequisite,and oxidization acting as a metabolic manifestation. This study provides unique approaches for further investigation of the formation mechanisms of heavy oils in general,and may provide some important insight for the exploration of shallow biogas in the area.     

土壤水环境中有机污染物生物处理过程模型研究

生物处理是一种经济有效处理土壤水环境中有机污染物的手段,本文在研究土地生物处理过程的基础上,建立了综合描述有机污染物在土壤－水－微生物系统中扩散、吸附／解吸、屏蔽和生物降解过程的数学模型。为确定模型中各参数在模型计算中的作用和相对重要性,进行了参数灵敏度分析,预计数学模型可以定量预测有机污染物进行土地生物处理所需的要时间和程度,为构建土地生物处理工程提供参考。     

东、南洞庭湖的径流、泥沙特征及冲淤规律

通过实地调查并对1957年以来水文、泥沙观测资料做系统分析和计算,探讨东、南洞庭湖出、入湖水量、沙量的年际和年内变化特征,以及长江下荆江段裁弯对湖区径流和泥沙的影响。提出了湖区泥沙汛淤枯冲的变化规律及水位升降与湖区泥沙冲淤的关系;论证了丰、平、枯年湖区淤积严重,面积日益缩小对径流的调节作用正在减弱。