Study on tissue culture forGelidium seedling

As seedling culture is a crucial factor for successful cultivation ofGelidium, the authors researched tissue culture technology for producing seedlings. The morphogeny and experimental ecology were observed and studied fully in 2–5 mm isolated tissue fragments. Regeneration, appearance of branching creepers and attaching structure and new erect seedlings production and development were studied. Fragments were sown on bamboo slice and vinylon rope. The seedlings were cultured 20–30 days indoor, then cultured in the sea, where the density of erect seedlings was 3–19 seedlings/cm², growth rate was 3.84% day. The frond arising from seedlings directly was up to 10 cm per year. The ecological conditions for regenerated seedlings are similar to the natural ones. The regenerated seedlings are suitable for raft culture in various sea areas.     

POPULATION DYNAMICS AND PRODUCTION OF NEANTHES JAPONIC A (IZUKA)IN A SHRIMP POND

During late February to mid-March, 1991, when mature N. japonica swarmed in the sea surface off Jimo County, Qingdao, seawater containing numerous early embryos was pumped into a nearby 2.7 ha. muddy-sand shrimp pond, where the embryos continued to develop. 800000 Penaeus chinensis seedlings were introduced into the pond on May 30, 1991. Monthly benthic samplings were carried out to determine the population dynamics and production of N. japonica in this atypical environ -ment.The density of N. japonica varied from 0 to 20400 ind./m2. The predation of P. chinensis was considered to be responsible for the mass mortality of N. japonica in June through July, when the worms were 2.2-3.3cm in length and lived in the top 2-4cm sediment. From August to September of 1991, the hottest period in the site, there was no substantial mortality of N. japonica as reported in earlier literature. This was explained by the deeper burrowing of the' worms that protected them from predation by shrimps.Monthly biomass ranged f     

Formation and early development of tetraspores of Polysiphonia urceolata (Rhodomelaceae, Rhodophyta)

Polysiphonia urceolata is one type of potential commercial red seaweeds used for breeding and cultivation, because of its significant biochemical and biomedical application. However, the information of breeding and seedling incubation for cultivation is limited, especially the early development. In this study, tetrasporohyte and gametophyte of P. urceolata were taken as the study materials in Huiquan Bay, Qingdao, China. The cleaned and sterilized tetrasporophytes and gametophytes were pre-cultured in sterilized seawater, then nurtured at 18℃, 25 μmol photons m-2 s-1 in 12:12 h (light:dark) photoperiod. Continuous observation under microscope showed that the early development consists of bipolar division stage and seedling stage. In the division stage, tetraspores germinate into bipolar sporelings that further differentiate into a colorless rhizoidal portion and a lightly pigmented upright shoot. The lightly pigmented rhizoidal cell develops to a rhizoid and the larger pigmented cell transforms to an erect axis. In the seedling stage, several quasi-protuberances appear on the erect axis and form juvenile seedlings. The results demonstrate the culture of P. urceolata from tetraspores under laboratory conditions.     

三角帆蚌室内流水育苗

三角帆蚌流水式人工育苗，采用亲蚌自然授精，静水采集钩介幼虫，流水育苗。在育苗室周围设防护堤，室内各育苗地用砖叠成，砖块间留有缝隙，使之成为一个有微流的水环境。以体长１０ｃｍ的鳙鱼采集钩介幼虫，作为宿主鱼。流水育苗操作简便，效果较好，每个小池（１．４４ｍ２）可培育稚蚌５０００～６０００只，高产者可达１万只。     

基于CryoSat-2数据反演2011～2017年波弗特海海冰厚度变化

利用CryoSat-2卫星测高数据反演波弗特海的海冰厚度,并利用2010~2013年10月份仰视声呐(ULS)和2011年冰桥计划（IceBridge）数据对结果进行精度评估。结果表明,测高反演的海冰吃水深度与ULS吃水深度差值的最大值和标准差分别为14 cm和4 cm;测高反演的海冰厚度与冰桥计划海冰厚度差值的平均值和标准差分别为2.7 cm和65.7 cm,优于Laxon(2013)研究结果（分别优化2.1 cm和6.6 cm）。在此基础上,研究2011~2017年波弗特海夏冬两季的海冰厚度变化,发现二者具有类似的分布特征,且冬季3月海冰覆盖范围更广,厚度更大;进一步分析2011~2017年3月份冬季海冰厚度年际变化,发现其呈整体下降趋势,且2012年最小,2014年最大。     

Global sea level change and thermal contribution

The global long-term sea level trend is obtained from the analysis of tide gauge data and TOPEX/Poseidon data. The linear trend of global mean sea level is highly non-umiform spatially, with an average rate of 2.2 mm year-1 in T/P sea-level rise from October 1992 to September 2002. Sea level change duc to temperature vanation (the thermosteric sea level) is discussed. The results are compared with TOPEX/Poseidon altimeter data in the same temporal span at different spatial scales. It is indicated that the ther-mal effect accounts for 86% and 73% of the observed seasonal variability in the northern and southern hemispheres, respectively. The TOPEX/Poseidon observed sea level lags behind the TSI, by 2 months in the zonal band of 40°-60° in both the northern and southern hemispheres. Systematic differences of about 1-2cm between TOPEX/Poseidon observations and thermosteric sea level data are obtained. The potential causes for these differences include water exchange among the atmosphere, land, and oceans, and some pos-sible deviations in thermosteric contribution estimates and geophysical corrections to the TOPEX/Poseidon data.     

Formation and early development of tetraspores of Polysiphonia urceolata （Rhodomelaceae, Rhodophyta）

Polysiphonia urceolata is one type of potential commercial red seaweeds used for breeding and cultivation, because of its significant biochemical and biomedical application. However, the information of breeding and seedling incubation for cultivation is limited, especially the early development. In this study, tetrasporohyte and gametophyte of P. urceolata were taken as the study materials in Huiquan Bay, Qingdao, China. The cleaned and sterilized tetrasporophytes and gametophytes were pre-cultured in sterilized seawater, then nurtured at 18°C, 25 μmol photons m⁻² s⁻¹ in 12:12 h (light:dark) photoperiod. Continuous observation under microscope showed that the early development consists of bipolar division stage and seedling stage. In the division stage, tetraspores germinate into bipolar sporelings that further differentiate into a colorless rhizoidal portion and a lightly pigmented upright shoot. The lightly pigmented rhizoidal cell develops to a rhizoid and the larger pigmented cell transforms to an erect axis. In the seedling stage, several quasi-protuberances appear on the erect axis and form juvenile seedlings. The results demonstrate the culture of P. urceolata from tetraspores under laboratory conditions. Supported by National Key Technology Support Program, Development Program of China (No.2006AA09Z21), National Natural Science Foundation of China (No. 40618001 and N_CUHK438/06) and Shandong Agricultural Seed Stock Breeding Project     

Isolation and cultivation of the vegetative cells ofPorphyra haitanensis

This research deals mainly with the use of the vegetative cells of a marine red algaPorphyra haitanensis (laver) as seeds and their culture into young thalli for further cultivation in the sea. The experimental process was as follows. Enzymatically isolated vegetative cells were attached to rope substrates and cultured in the laboratory for a month until they became about 0.2–0.5 cm long sporelings and were then attached (7–15 sporelings per cm of rope) to nets for removal to the open sea for cultivation. A month after culturing, the thalli reached a maximum length of 50 cm (average of 20–30 cm). The thalli grew faster as the water temperature dropped from 21°C to 17°C. It was proved that the vegetative cells isolated from a small thallus about 5 cm long could grow up into normal thalli after being kept frozen for a year. The results of this study show that vegetative cells can be used as new seeds to simplify the production of laver sporelings. This technique is a significant advance in the field ofPorphyra culture.     

Tidal current observation in the southern Yellow Sea in the summers of 2001 and 2003

Direct current observations in the Yellow Sea interior are very scarce due to intense fishing and trawling activities. Most previous studies on tides in the area were based on coastal measurements or satellite altimeter sea levels and have not been rigorously compared with direct measurements. In this paper, tidal currents are studied with current profiles from three bottom-moored Sontek Acoustic Doppler Profilers （ADPs） deployed in the southern Yellow Sea in summer of 2001 and 2003. The measured current series were dominated by tidal currents. Maximum velocities are between 40-80 cm/s at the mooring stations. M2 current is the most dominant primary tidal constituent, while MS4 and M4 are the most significant shallow water tides with much smaller amplitudes than the primary tides.     

秘鲁外海鲯鳅生物学特性及资源分布初步研究

根据2012年5~7月东南太平洋秘鲁外海(探捕海域在83°W~87°W、10°S~14°S)鲯鳅资源的探捕调查资料,对其生物学特性作了初步分析。结果表明:秘鲁外海鲯鳅的叉长范围为31~121cm,优势叉长为30~50cm,占57.4%;在个体大小的空间分布上,探捕海域北部比南部个体略微偏大;雌性叉长(l)与体质量(m)关系式为m=5×10-4·l2.236 2(R2=0.940 5;n=104 9),雄性为m=5×10-4l2.209 6(R2=0.958 0,n=851);雌性鲯鳅比例稍高,占55.2%。探捕海域鲯鳅鱼资源密度较高,CPUE值范围为2~23.6 kg/(人·h),平均为11.6 kg/(人·h);不同区域资源密度有所差异,其中CPUE最高的区域在84°W~86°W、12°30′S~13°30′S附近;探捕期间6月份的最高平均CPUE为16.37 kg/(人·h)。     

水流速率对全缘马尾藻幼孢子体生长和生理活性的影响

在0、0.5、1、2、4、8 cm/s 等5 种不同水流速率下培养全缘马尾藻（Sargassum integerrimum）幼孢子体,研究水流速率对幼孢子体质量、叶绿素a 含量、SOD 活力、CAT 活力和蛋白质浓度等生理指标的影响.结果表明,以1、2、4 cm/s 流速的幼孢子体质量增长最快,3 种流速的增长差异不具统计学意义（P〉0.05）.在生理指标方面：叶绿素a 含量以1、2、4 cm/s 流速最高,3 种流速的含量差异不具统计学意义（P〉0.05）;SOD 比活性以流速0.5、1、2 cm/s 的活力最低,各组间差异不具统计学意义（P〉0.05）;CAT 比活性以流速1、2 cm/s 的活力最低,组间差异不具统计学意义（P〉0.05）;蛋白质含量以1、2、4 cm/s 流速最大,组间差异具统计学意义（P〈0.05）.水流速率对体质量增长和各生化指标均产生显著的影响,以流速1～4 cm/s 培育全缘马尾藻培幼孢子体较合适.     

Ecological effects of co-culturing sea cucumber Apostichopus japonicus (Selenka) with scallop Chlamys farreri in earthen ponds

Monthly changes in sedimentation and sediment properties were studied for three different culture treatments: sea cucumber monoculture (Mc), sea cucumber and scallop polyculture (Ps-c) and scallop monoculture (Ms). Results indicated that the survival rate of sea cucumber was significantly higher in Ps-c cultures than in Mc cultures. Sea cucumber yield was 69.6% higher in Ps-c culture than in Mc culture. No significant differences in body weight and scallop shell length were found between Ps-c and Ms cultures. The mean sedimentation rate of total particulate matter (TPM) was 72.2 g/(m2 d) in Ps-c cultures, with a maximum of 119.7 g/(m2 d), which was markedly higher than that of Mc (mean value). Sedimentation rates of organic matter (OM), total organic carbon (TOC), total nitrogen (TN) and total phosphorus (TP) in Ps-c cultures were also significantly higher than those in Mc cultures. TOC and TN contents of sediment increased rapidly in the first 5 months in Ms cultures and remained at a high level. TOC and TN contents in Mc and Ps-c cultures decreased during sea cucumber feeding seasons and increased during sea cucumber dormancy periods (summer and winter). The study demonstrates that co-culture of sea cucumber and scallop in earthen ponds is an alternative way to alleviate nutrient loads and improve water quality in coastal aquaculture systems. Moreover, it provides the additional benefit of an increased sea cucumber yield.     

舌状蜈蚣藻Grateloupia livida(Harv.)Yamada生殖器官的研究

本种为我国南方常见一种经济红藻。藻体直立,叶片窄带状,单条或叉状,全缘或有小育枝,体下部淅细成柄状。藻体不中空。我们发现它在生殖器官发育过程中也出现三个新特徵:1、少数果胞枝与助细胞可在同一的生殖枝丛中形成,2、少数果胞枝枝丛中可出现2个果胞枝;3、少数助细胞枝丛中可出现2个助细胞。     

ASPECTS OF IRON NUTRITION IN MACROALGAE ULVA PERTUSA (CHLOROPHYTA) UNDER IRON STRESS

ＩＮＴＲＯＤＵＣＴＩＯＮＩｒｏｎｉｓａｎｉｍｐｏｒｔａｎｔｎｕｔｒｉｔｉｏｎａｌｅｌｅｍｅｎｔｉｎａｌｇａｅｊｕｓｔａｓｉｔｉｓｉｎｈｉｇｈｐｌａｎｔｍｅｔａｂｏｌｉｃｐａｔｈｗａｙｓ.Ｐｈｏｔｏｓｙｎｔｈｅｓｉｓｉｓｄｅｐｅｎｄｅｎｔｏｎｔｈｉｓｅｌｅｍｅｎｔｂｅｃａｕｓｅｃｈｌｏｒｏｐｈｙｌｌｓｙｎｔｈｅｓｉｓｒｅｑｕｉｒｅｓｉｒｏｎｎｕｔｒｉｔｉｏｎ .Ｉｒｏｎｉｓａｌｓｏｅｓｓｅｎｔｉａｌｆｏｒｃｙｔｏｃｈｒｏｍｅｓ,ｆｅｒｒｅｄｏｘｉｎ ,ａｎｄＦｅ ｓｐｒｏｔｅｉｎ .Ｉｔｓｂｉｏｃｈｅｍｉ…     

粉蕉离体的快速繁殖技术

以粉蕉的吸芽为试材 ,进行了离体快速繁殖研究 ,结果表明 :以改良MS +BA4 .0mg/L(单位下同 ) +AD5.0 +NAA0 .1作诱导培养基 ,外植体褐变率低 ,芽的诱导率高 ,且芽的生长良好 ;以改良MS +BA3- 6 +NAA0 .1为继代培养基 ,可以获得较高的不定芽增殖率 ,在增殖过程中 ,给予一定的低温处理和变化调整BA的用量 ,可以保持较高的增殖率 (2 .6倍以上 ) ,而且芽的长势好 ;生根培养以 1/ 2MS +IBA1.0 +NAA0 .2 +AC1为宜。     

Absolute calibration of HY-2A and Jason-2 altimeters for sea surface height using GPS buoy in Qinglan,China

GPS buoy methodology is one of the main calibration methodologies for altimeter sea surface height calibration.This study introduces the results of the Qinglan calibration campaign for the HY-2 A and Jason-2 altimeters.It took place in two time slices;one was from August to September 2014,and the other was in July 2015.One GPS buoy and two GPS reference stations were used in this campaign.The GPS data were processed using the real-time kinematic(RTK)technique.The final error budget estimate when measuring the sea surface height(SSH)with a GPS buoy was better than 3.5 cm.Using the GPS buoy,the altimeter bias estimate was about-2.3 cm for the Jason-2 Geophysical Data Record(GDR)Version 'D' and from-53.5 cm to-75.6 cm for the HY-2 A Interim Geophysical Data Record(IGDR).The bias estimates for Jason-2 GDR-D are similar to the estimates from dedicated calibration sites such as the Harvest Platform,the Crete Site and the Bass Strait site.The bias estimates for HY-2 A IGDR agree well with the results from the Crete calibration site.The results for the HY-2 A altimeter bias estimated by the GPS buoy were verified by cross-calibration,and they agreed well with the results from the global analysis method.     

鱼汁、海泥抽出液和人尿对小环藻生长的影响

在室内条件下，通过单因子实验和正交实验的方法，研究了鱼汁、海泥抽出液和人尿对小环藻（Cyclotella sp．）种群生长的影响。结果表明，350mL的培养体积下，当鱼汁添加量为20mL时，小环藻生长效果最好，相对生长率（K）较对照组升高了33．3％，平均倍增时间（G）降低了34．9％，但随着浓度升高，藻细胞开始出现死亡。海泥抽出液添加量为20mL时，K值最大，较对照组增加35．6％，G值下降了25．15％。人尿添加量为10mL时，K值最大，较对照组增加17．9％，G值下降了15．77％。统计分析表明，鱼汁、海泥抽出液和人尿对小环藻实验种群的生长均有极显著影响。海泥抽出液对小环藻影响大，其次是鱼汁和人尿。正交实验表明，在鱼汁、海泥抽出液和人尿的添加量分别为2．5、15．0和1．0mL的组合实验组，小环藻的生长效果最好。     

鱼汁、海泥抽出液和人尿对小环藻生长的影响

在室内条件下,通过单因子实验和正交实验的方法,研究了鱼汁、海泥抽出液和人尿对小环藻(Cyclotella sp.)种群生长的影响。结果表明,350 mL的培养体积下,当鱼汁添加量为2~3 mL时,小环藻生长效果最好,相对生长率(K)较对照组升高了33.3%,平均倍增时间(G)降低了34.9%,但随着浓度升高,藻细胞开始出现死亡。海泥抽出液添加量为20 mL时,K值最大,较对照组增加35.6%,G值下降了25.15%。人尿添加量为10 mL时,K值最大,较对照组增加17.9%,G值下降了15.77%。统计分析表明,鱼汁、海泥抽出液和人尿对小环藻实验种群的生长均有极显著影响。海泥抽出液对小环藻影响大,其次是鱼汁和人尿。正交实验表明,在鱼汁、海泥抽出液和人尿的添加量分别为2.5、15.0和1.0 mL的组合实验组,小环藻的生长效果最好。     

A soil temperature control system for sapling study in alpine region

A soil temperature control system was designed for sapling study in alpine region and tested in summer, 2009. The system consisted of a power switch, voltage regulator, microcomputer timer, safety relays, temperature control device, temperature sensors, heating cables, fireproofing plastic pipes (PVC), 108 heavy-duty plastic containers and seedlings. The heating cables were held in six 2-layer PVC frames with 25 cm wide, 320 cm long and 25 cm high and three 1-layer frames with 25 cm wide and 320 cm long for 15°C soil temperature treatment, half of the 2-layer frames were used for 20°C and 25°C soil temperature treatments, respectively. Each of the frames was installed at each of ditches with 30 cm wide, 330 cm long and 30 cm deep in size. 12 seedling containers with 20 cm top diameter, 18cm bottom diameter and 25 cm high were homogenously placed at each of the ditches, and spaces between the containers were filled with natural soil. The system was economic, and could increase soil temperatures obviously and uniformly, the maximal and minimal standard errors of soil temperatures were ±0.28 and ±0.05°C at 10cm depth in the containers within each of all the ditches. In the system, aboveground environment was natural, diurnal and monthly soil temperatures varied with changing air temperature, the research results may be better to know the eco-physiological and growth responses of alpine saplings/seedlings to soil warming than that in greenhouse, laboratory, infrared heat lamp and open top chamber.