Abschätzung der Eignung einer molekularbiologischen Methode zur Charakterisierung der Grundwasserbiozönose

Assessing the Suitability of a Molecularbiological Method To Characterise the Microbial Populations in Groundwater A molecularbiological technique was used to characterise the bacterial community structure of groundwater habitats. This method consists of the isolation of bacterial DNA from the samples, amplification of 16S rDNA by PCR (polymerase chain reaction), and separation of the amplified DNA by DGGE (denaturing gradient gel electrophoresis). By using more specific primer combinations in the PCR instead of universal eubacterial primers, also groups of microorganisms (Proteobacteria, sulfate reducer, Archaea) were determined. The resulting DGGE patterns that reflect the microbial diversity are compared and differences or similarities evaluated. In the present studies, groundwater from different sites (bank filtrate, artificially recharged groundwater, and natural groundwater) and with changing redox milieus (aerobic, anaerobic) were investigated as well as the solid aquifer material. Besides, samples were taken from the different stages of artificial groundwater recharge, i.e., from surface water to the drain tile. Samples from groundwater derived from sites with different hydrogeochemical or hydrological conditions like bank filtrate and recharged groundwater revealed great differences in DGGE patterns indicating a characteristic species composition in these habitats, while samples taken at different times from the same groundwater showed only small seasonal variations. Clearly different patterns were also found for groundwater and the adjacent solid material as well as for anaerobic and aerobic groundwaters. Looking at artificial groundwater recharge, almost identical patterns were found in raw water and samples from gravel and sand filtration. DGGE patterns from the resulting groundwater indicated a total change in community structure during underground passage. By using group specific primers, Desulfovibrionaceae, Desulfobacteriaceae, and Archaea could be detected in anaerobic groundwaters.The molecularbiological approach described here gives an increasingly comprehensive and more precise picture of the microbial population of different environments. It is especially suitable to compare the community structure from different habitats or to analyse changes for example due to environmental stress at the same site.     

Verhalten ausgewählter Arzneimittel bei der künstlichen Grundwasseranreicherung – Eliminierung und Effekte auf die mikrobielle Besiedlung

Behaviour of Some Pharmaceuticals during Artificial Groundwater Recharge – Elimination and Effects on Microbiology The behaviour of bezafibrate, carbamazepine, clofibric acid, diclofenac, ibuprofen, and gemfibrozil during artificial groundwater recharge was investigated with different test systems simulating field conditions. The given concentrations of the pharmaceuticals were 100 μg/L in the influent of the systems. Concentrations in the influent as well as in the effluent were measured by GC‐MS. These column experiments indicated a significant elimination of bezafibrate, diclofenac, and ibuprofen (60 to 80%) during slow sand filtration. The results showed a moderate elimination of clofibric acid and gemfibrozil (40 to 60%) but a rather low elimination of carbamazepine (<40%). The adaptation times until the elimination processes started were about 5 days. Only the elimination of carbamazepine needed a lag phase up to 17 days. Additional column experiments with groundwater model systems indicated a high persistence of pharmaceuticals under aerobic and anaerobic groundwater conditions. The elimination was less than 20%. Only diclofenac was eliminated with rates between 60% and 80% in aerobic systems and between 40% and 60% in anaerobic systems. Analysis of eubacterial 16S‐rDNA by PCR and DGGE demonstrated changes in the microbial community structure in slow sand filters after application of pharmaceuticals. Adaptation processes may cause these changes, e.g. the appearance or disappearance of single species. Also differences between the populations of water and of the solid phase in slow sand filters could be demonstrated by DGGE pattern.     

Chemical water types and their distribution in space and time in the Amsterdam dune-water catchment area with artificial recharge

The geology, hydrology and types of groundwater exploitation of the Amsterdam dune-water catchment area with artificial recharge of water from the river Rhine are briefly introduced. A classification of natural and artificial watertypes in the catchment is presented together with their genesis and distribution in space and time. The natural, vertical, hydrochemical zonation in fresh, brackish and salt groundwaters has been disturbed by decades of overdraft and the subsequent start of artificial recharge since 1957. Various mixed-water types resulted. A detailed case study of a part of the catchment area (around the Barnaart-Schuster Canal) covers a period of about 50 years. The decisive influence of geological inhomogeneities on the patterns of saltwater encroachment during the period of overdraft and on the patterns of infiltration of river water into the deep aquifer after the start of artificial recharge are demonstrated. The shifts in the subsurface positions of the various water types due to changes in the groundwater management of the catchment area are elucidated. They give an example of displacement and dispersion phenomena under field conditions.     

Groundwater Microbial Communities Along a Generalized Flowpath in Nomhon Area,Qaidam Basin,China

Spatial distribution (horizonal and vertical) of groundwater microbial communities and the hydrogeochemistry in confined aquifers were studied approximately along the groundwater flow path from coteau to plain in the Nomhon area, Qinghai‐Tibet plateau, China. The confined groundwater samples at different depths and locations were collected in three boreholes through a hydrogeological section in this arid and semi‐arid area. The phylogenetic analysis of 16S rRNA genes and multivariate statistical analysis were used to elucidate similarities and differences between groundwater microbial communities and hydrogeochemical properties. The integrated isotopic geochemical measurements were applied to estimate the source and recharge characteristics of groundwater. The results showed that groundwater varied from fresh to saline water, and modern water to ancient water following the flowpath. The recharge characteristics of the saline water was distinct with that of fresh water. Cell abundance did not vary greatly along the hydrogeochemical zonality; however, dissimilarities in habitat‐based microbial community structures were evident, changing from Betaproteobacteria in the apex of alluvial fan to Gammaproteobacteria and then to Epsilonproteobacteria in the core of the basin (alluvial‐lacustrine plain). Rhodoferax, Hydrogenophaga, Pseudomonas, and bacterium isolated from similar habitats unevenly thrived in the spatially distinct fresh water environments, while Sulfurimonas dominanted in the saline water environment. The microbial communities presented likely reflected to the hydrogeochemical similarities and zonalities along groundwater flowpath.     

Biogeographical distribution and diversity of bacterial and archaeal communities within highly polluted anoxic marine sediments from the marmara sea

Physicochemical and microbiological characterization of anoxic sediments taken from seven highly polluted sites of the Marmara Sea was carried out. The 16S rRNA based microbial community structure analyses were performed using domain-specific PCR followed by denaturant gradient gel electrophoresis (DGGE) and sequencing of characteristic bands. The results showed that the microbial communities in these sediments were diverse and evenly distributed. Relating the prokaryotic and geochemical variables through statistical tools revealed that the microbial diversity in the sediments significantly related to depth, and S, Mn and Fe content of the sediments. Fermentative bacteria, denitrifying bacteria and hydrogenotrophic methanogens were dominant whereas sulfate reducing bacteria were absent in the DGGE patterns. This unusual microbial community structure implied that the newly discovered anaerobic methane oxidation coupled to denitrification process may occur in these subseafloor environments.     

The impact of shrimp farming effluent on bacterial communities in mangrove waters, Ceará, Brazil

The effects of shrimp farm effluents on bacterial communities in mangroves have been infrequently reported. Classic and molecular biology methods were used to survey bacterial communities from four mangroves systems. Water temperature, salinity, pH, total heterotrophic bacteria and maximum probable numbers of Vibrio spp. were investigated. Genetic profiles of bacterial communities were also characterized by polymerase chain reaction (PCR) amplification of eubacterial and Vibrio 16S rDNA using denaturing gradient gel electrophoresis (DGGE). Highest heterotrophic counts were registered in the mangrove not directly polluted by shrimp farming. The Enterobacteriaceae and Chryseomonas luteola dominated the heterotrophic isolates. Vibrio spp. pathogenic to humans and shrimps were identified. Eubacterial genetic profiles suggest a shared community structure independent of mangrove system. Vibrio genetic profiles were mangrove specific. Neither microbial counts nor genetic profiling revealed a significant decrease in species richness associated with shrimp farm effluent. The complex nature of mangrove ecosystems and their microbial communities is discussed.     

PAHs contamination and bacterial communities in mangrove surface sediments of the Jiulong River Estuary, China

Sixteen sediment samples collected from eight transects in a mangrove swamp of the Jiulong River Estuary, Fujian, China were investigated for their content of polycyclic aromatic hydrocarbons (PAHs) and the biodegradation potential of the indigenous microorganisms. The bacterial community structures in the mangrove sediments and in enrichment cultures were also investigated. The results showed that the total PAHs concentration of mangrove sediments ranged from 280 to 1074 ng g(-1) dry weight, that the PAHs composition pattern in the mangrove sediments was dominated by high molecular weight PAH components (4-6 rings), and that Benzo[ghi]perylene and Indeno[1,2,3-cd]pyrene were the most dominant at different stations. Abundant PAH-degrading bacteria were found in all the stations, the values of phenanthrene-degrading bacteria ranged from 5.85 x 10(4) to 7.80 x 10(5) CFU g(-1) dry weight, fluoranthene-degrading bacteria ranged from 5.25 x 10(4) to 5.79 x 10(5) CFU g(-1) dry weight, pyrene-degrading bacteria ranged from 3.10 x 10(4) to 6.97 x 10(5) CFU g(-1) dry weight and the benzo(a)pyrene-degrading bacteria ranged from 5.25 x 10(4) to 7.26 x 10(5) CFU g(-1) dry weight. DGGE analysis of PCR-amplified 16S rDNA gene fragments confirmed that there was a remarkable shift in the composition of the bacterial community due to the addition of the different model PAH compound phenanthrene (three ring PAH), fluoranthene(four ring PAH), pyrene(four ring PAH) and benzo(a)pyrene(five ring PAH) during enrichment batch culture. Eleven strains were obtained with different morphology and different degradation ability. The presence of common bands for microbial species in the cultures and in the native mangrove sediment DNA indicated that these strains could be potential in situ PAH-degraders.     

Groundwater isoscapes in a montane headwater catchment show dominance of well‐mixed storage

We conducted an integrated groundwater–surface water monitoring programme in a 3.2‐km² experimental catchment in the Scottish Highlands by sampling all springs, seepages, and wells in six, spatially extensive synoptic surveys over a 2‐year period. The catchment has been glaciated, with steep hillslopes and a flat valley bottom. There is around 70% glacial drift cover in lower areas. The solid geology, which outcrops at higher elevations, is granite and metamorphic schist. The springs and seepages generally occur at the contact between the solid geology and drift or at breaks of slopes in the valley bottom. Samples were analysed for stable isotopes, Gran alkalinity and electrical conductivity. Despite the surveys encompassing markedly different antecedent conditions, the isotopic composition of groundwater at each location exhibited limited temporal variability, resulting in a remarkable persistence of spatial patterns indicating well‐mixed shallow, groundwater stores. Moreover, line‐conditioned excess values derived from the isotope data indicated no evidence of fractionation affecting the groundwater, which suggests that most recharge occurs in winter. The alkalinity and electrical conductivity of groundwater reflected geological differences in the catchment, being highest where more weatherable calcareous rocks outcrop at higher altitudes in the catchment. Springs draining these areas also had the most variable isotope composition, which indicated that they have shorter residence times than the drift covered part of the catchment. The study showed that even in geologically heterogeneous upland catchments, groundwater can be characterized by a consistent isotopic composition, reflecting rapid mixing in the recharge zone. Our work, thus, emphasizes the critical role of groundwater in upland catchments and provides tracer data that can help constrain quantitative groundwater models.     

Estimating groundwater recharge from water isotope (δ2H, δ18O) depth profiles in the Densu River basin,Ghana

Abstract

Accurate estimation of groundwater recharge is essential for the proper management of aquifers. A study of water isotope (δ²H, δ¹⁸O) depth profiles was carried out to estimate groundwater recharge in the Densu River basin in Ghana, at three chosen observation sites that differ in their altitude, geology, climate and vegetation. Water isotopes and water contents were analysed with depth to determine water flow in the unsaturated zone. The measured data showed isotope enrichment in the pore water near the soil surface due to evaporation. Seasonal variations in the isotope signal of the pore water were also observed to a depth of 2.75 m. Below that depth, the seasonal variation of the isotope signal was attenuated due to diffusion/dispersion and low water flow velocities. Groundwater recharge rates were determined by numerical modelling of the unsaturated water flow and water isotope transport. Different groundwater recharge rates were computed at the three observation sites and were found to vary between 94 and 182 mm/year (± max. 7%). Further, the approximate peak-shift method was applied to give information about groundwater recharge rates. Although this simple method neglects variations in flow conditions and only considers advective transport, it yielded mean groundwater recharge rates of 110–250 mm/year (± max. 30%), which were in the same order of magnitude as computed numerical modelling values. Integrating these site-specific groundwater recharge rates to the whole catchment indicates that more water is potentially renewed than consumed nowadays. With increases in population and irrigation, more clean water is required, and knowledge about groundwater recharge rates – essential for improving the groundwater management in the Densu River basin – can be easily obtained by measuring water isotope depth profiles and applying a simple peak-shift approach.

Citation Adomako, D., Maloszewski, P., Stumpp, C., Osae, S. & Akiti, T. T. (2010) Estimating groundwater recharge from water isotope (δ²H, δ¹⁸O) depth profiles in the Densu River basin, Ghana. Hydrol. Sci. J. 55(8), 1405–1416.     

Storage-discharge characteristics of an active rock glacier catchment in the Innere Ölgrube,Austrian Alps

The active rock glacier “Innere Ölgrube” and its catchment area (Ötztal Alps, Austria) are assessed using various hydro(geo)logical tools to provide a thorough catchment characterization and to quantify temporal variations in recharge and discharge components. During the period from June 2014 to July 2018, an average contribution derived from snowmelt, ice melt and rainfall of 35.8%, 27.6% and 36.6%, respectively, is modelled for the catchment using a rainfall-runoff model. Discharge components of the rock glacier springs are distinguished using isotopic data as well as other natural and artificial tracer data, when considering the potential sources rainfall, snowmelt, ice melt and longer stored groundwater. Seasonal as well as diurnal variations in runoff are quantified and the importance of shallow groundwater within this rock glacier-influenced catchment is emphasized. Water derived from ice melt is suggested to be provided mainly by melting of two small cirque glaciers within the catchment and subordinately by melting of permafrost ice of the rock glacier. The active rock glacier is characterized by a layered internal structure with an unfrozen base layer responsible for groundwater storage and retarded runoff, a main permafrost body contributing little to the discharge (at the moment) by permafrost thaw and an active layer responsible for fast lateral flow on top of the permafrost body. Snowmelt contributes at least 1/3rd of the annual recharge. During droughts, meltwater derived from two cirque glaciers provides runoff with diurnal runoff variations; however, this discharge pattern will change as these cirque glaciers will ultimately disappear in the future. The storage-discharge characteristics of the investigated active rock glacier catchment are an example of a shallow groundwater aquifer in alpine catchments that ought to be considered when analysing (future) river runoff characteristics in alpine catchments as these provide retarded runoff during periods with little or no recharge.     

与微囊藻胞外多糖降解相关的微生物菌群分析

从铜绿微囊藻培养液中提取胞外多糖,化学分析显示其为含8.3%蛋白质的酸性杂多糖.以胞外多糖为碳源接种自然水体的菌群进行富集培养,分析胞外多糖的微生物降解过程及降解菌的组成.结果表明,接种太湖水华期微生物富集菌群后,多糖立即开始被降解,大约在18d后多糖降解过程显著减慢,37d后仍有一部分多糖未能被降解.这些结果说明在自然界中微囊藻胞外多糖是可以被微生物降解的.比较不同水体的菌群对多糖的降解能力后显示,降解菌群只存在于微囊藻水华暴发的阶段.变性梯度凝胶电泳(DGGE)结果显示在整个降解过程中,降解菌群的组成未发生显著变化.对DGGE条带中的DNA片段进行序列分析和系统发育分析表明降解菌群包含以下几类:鞘氨醇单胞菌属(Sphingomonas)3个,褐螺菌属(Phaeospirillum)1个,假单胞菌属(Pseudomonas)1个,红环菌科(Rhodoeyclaceae)1个,Hylemonella(无译名)1个,分枝杆菌属(Mycobacterium)1个.     

Hydrogeochemical Evolution Along Groundwater Flow Paths in the Manas River Basin,Northwest China

The impacts of long-term pumping on groundwater chemistry remain unclear in the Manas River Basin, Northwest China. In this study, major ions within five surface water and 105 groundwater samples were analyzed to identify hydrogeochemical processes affecting groundwater composition and evolution along the regional-scale groundwater flow paths using the multivariate techniques of hierarchical cluster analysis (HCA) and principal components analysis (PCA) and traditional graphical methods for analyzing groundwater geochemistry. HCA classified the groundwater samples into four clusters (C1 to C4). PCA reduced the dimensionality of geochemical data into three PCs, which explained 86% of the total variance. The results of HCA and PCA were used to identify three zones: “recharge,” “transition,” and “discharge.” In the recharge zone the groundwater type is Ca-HCO₃-SO₄ and is primarily impacted by the dissolution of calcite and silicate weathering. In the transition zone the groundwater type is Ca-HCO₃-SO₄-Cl and is impacted by rock dissolution and reverse ion exchange. In the discharge zone the groundwater type is Na-Cl and is impacted by evaporation and reverse ion exchange. In addition, anthropogenic activities impact the groundwater chemistry in the study area. The groundwater type generally changes from Ca-HCO₃-SO₄ in the recharge area to Na-Cl in the discharge area along the regional-scale groundwater flow paths. This study provides a process-based knowledge for understanding the interaction of groundwater flow patterns and geochemical evolution within the Manas River Basin.     

Application of Multivariate Statistical Techniques for Characterization of Groundwater Quality in the Coastal Aquifer of Nador,Tipaza (Algeria)

The study focuses on the characterization of the groundwater salinity on the Nador coastal aquifer (Algeria). The groundwater quality has undergone serious deterioration due to overexploitation. Groundwater samplings were carried out in high and low waters in 2013, in order to study the evolution of groundwater hydrochemistry from the recharge to the coastal area. Different kinds of statistical analysis were made in order to identify the main hydrogeochemical processes occurring in the aquifer and to discriminate between different groups of groundwater. These statistical methods provide a better understanding of the aquifer hydrochem-istry, and put in evidence a hydrochemical classification of wells, showing that the area with higher salinity is located close to the coast, in the first two kilometers, where the salinity gradually increases as one approaches the seaside and suggests the groundwater salinization by sea-water intrusion.     

Analysis of community structure of a microbial consortium capable of degrading benzo(a)pyrene by DGGE

A microbial consortium was obtained by enrichment culture of sea water samples collected from Botan oil port in Xiamen, China, using the persistent high concentration of a mixture of polycyclic aromatic hydrocarbons enrichment strategy. Denaturing gradient gel electrophoresis (DGGE) was used to investigate the bacterial composition and community dynamic changes based on PCR amplification of 16S rRNA genes during batch culture enrichment. Using the spray-plate method, three bacteria, designated as BL01, BL02 and BL03, which corresponded to the dominant bands in the DGGE profiles, were isolated from the consortium. Sequence analysis showed that BL01, BL02 and BL03 were phylogenetically close to Ochrobactrum sp., Stenotrophomonas maltophilia and Pseudomonas fluorescens, respectively. The degradation of benzo(a)pyrene (BaP), a model high-molecular-weight polycyclic aromatic hydrocarbon (HMW PAH) compound was investigated using individual isolates, a mixture of the three isolates, and the microbial consortium (BL) originally isolated from the oil port sea water. Results showed that the order of degradative ability was BL > the mixture of the three isolates > individual isolates. BL degraded 44.07% of the 10 ppm BaP after 14 days incubation, which showed the highest capability for HMW PAH compound degradation.Our results revealed that this high selective pressure strategy was feasible and effective in enriching the HMW PAH-degraders from the original sea water samples.     

Using isotopes to understand landscape-scale connectivity in a groundwater-dominated,lowland catchment under drought conditions

The Demnitzer Millcreek catchment (DMC), is a 66 km² long-term experimental catchment located 50 km SE of Berlin. Monitoring over the past 30 years has focused on hydrological and biogeochemical changes associated with de-intensification of farming and riparian restoration in the low-lying landscape dominated by rain-fed farming and forestry. However, the hydrological function of the catchment, which is closely linked to nutrient fluxes and highly sensitive to climatic variability, is still poorly understood. In the last 3 years, a prolonged drought period with below-average rainfall and above-average temperatures has resulted in marked hydrological change. This caused low soil moisture storage in the growing season, agricultural yield losses, reduced groundwater recharge, and intermittent streamflows in parts of an increasingly disconnected channel network. This paper focuses on a two-year long isotope study that sought to understand how different parts of the catchment affect ecohydrological partitioning, hydrological connectivity and streamflow generation during drought conditions. The work has shown the critical importance of groundwater storage in sustaining flows, basic in-stream ecosystem services and the dominant influence of vegetation on groundwater recharge. Recharge was much lower and occurred during a shorter window of time in winter under forests compared to grasslands. Conversely, groundwater recharge was locally enhanced by the restoration of riparian wetlands and storage-dependent water losses from the stream to the subsurface. The isotopic variability displayed complex emerging spatio-temporal patterns of stream connectivity and flow duration during droughts that may have implications for in-stream solute transport and future ecohydrological interactions between landscapes and riverscapes. Given climate projections for drier and warmer summers, reduced and increasingly intermittent streamflows are very likely not just in the study region, but in similar lowland areas across Europe. An integrated land and water management strategy will be essential to sustaining catchment ecosystem services in such catchment systems in future.     

Perspectives of artificial recharge of groundwater in southern European Russia

The current state of the problem of artificial recharge of groundwater in Russia and other countries is analyzed. The factors that govern the zoning by the conditions of artificial groundwater recharge are considered. The territory of the southern European Russia was zoned by the conditions of artificial groundwater recharge and the results of this zoning were used to compile maps of perspectives of artificial groundwater recharge in southern European Russia.     

Estimating groundwater recharge for an arid karst system using a combined approach of time‐lapse camera monitoring and water balance modelling

Groundwater is the principal water resource in semi‐arid and arid environments. Therefore, quantitative estimates of its replenishment rate are important for managing groundwater systems. In dry regions, karst outcrops often show enhanced recharge rates compared with other surface and sub‐surface conditions. Areas with exposed karst features like sinkholes or open shafts allow point recharge, even from single rainfall events. Using the example of the As Sulb plateau in Saudi Arabia, this study introduces a cost‐effective and robust method for recharge monitoring and modelling in karst outcrops. The measurement of discharge of a representative small catchment (4.0 · 10⁴ m²) into a sinkhole, and hence the direct recharge into the aquifer, was carried out with a time‐lapse camera. During the monitoring period of two rainy seasons (autumn 2012 to spring 2014), four recharge events were recorded. Afterwards, recharge data as well as proxy data about the drying of the sediment cover are used to set up a conceptual water balance model. The model was run for 17 years (1971 to 1986 and 2012 to 2014). Simulation results show highly variable seasonal recharge–precipitation ratios between 0 and 0.27. In addition to the amount of seasonal precipitation, this ratio is influenced by the interannual distribution of rainfall events. Overall, an average annual groundwater recharge for the doline (sinkhole) catchment on As Sulb plateau of 5.1 mm has estimated for the simulation period. Copyright © 2015 John Wiley & Sons, Ltd.     

Groundwater recharge in the Akaki catchment,central Ethiopia: evidence from environmental isotopes (δ18O, δ2H and 3H) and chloride mass balance

Recharge patterns, possible flow paths and the relative age of groundwater in the Akaki catchment in central Ethiopia have been investigated using stable environmental isotopes δ¹⁸O and δ²H and radioactive tritium (³H) coupled with conservative chloride measurements. Stable isotopic signatures are encoded in the groundwater solely from summer rainfall. Thus, groundwater recharge occurs predominantly in the summer months from late June to early September during the major Ethiopian rainy season. Winter recharge is lost through high evaporation–evapotranspiration within the unsaturated zone after relatively long dry periods of high accumulated soil moisture deficits. Chloride mass balance coupled with the isotope results demonstrates the presence of both preferential and piston flow groundwater recharge mechanisms. The stable and radioactive isotope measurements further revealed that groundwater in the Akaki catchment is found to be compartmentalized into zones. Groundwater mixing following the flow paths and topography is complicated by the lithologic complexity. An uncommon, highly depleted stable isotope and zero‐³H groundwater, observed in a nearly east–west stretch through the central sector of the catchment, is coincident with the Filwoha Fault zone. Here, deep circulating meteoric water has lost its isotopic content through exchange reactions with CO₂ originating at deeper sources or it has been recharged with precipitation from a different rainfall regime with a depleted isotopic content. Copyright © 2006 John Wiley & Sons, Ltd.     

Impacts of human activities on recharge in a multilayered semiarid aquifer (Campo de Cartagena,SE Spain)

The development of intense agriculture in semiarid areas modifies intensity and spatial distribution of groundwater recharge by summing irrigation return flow to limited rainfall infiltration. Environmental tracers provide key information, but their interpretation is complicated by more complex groundwater flow patterns. In multilayered aquifers, the real origin of the groundwater samples is hard to assess because of local mixing processes occurring inside long‐screened boreholes. We use environmental tracers (¹⁴C, ¹³C, ²H, ¹⁸O, ³H) to investigate the long‐term evolution of recharge in the five‐layer Campo de Cartagena aquifer in South‐Eastern Spain, in addition to high‐resolution temperature loggings to identify the depth of origin of groundwater. Despite the complex background, this methodology allowed a reliable interpretation of the geochemistry and provided a better understanding of the groundwater flow patterns. The tritium method did not give good quantitative results because of the high variability of the recharge signal but remained an excellent indicator of recent recharge. Nonetheless, both pre‐anthropization and post‐anthropization recharge regime could be identified and quantified by radiocarbon. Before the development of agriculture, recharge varied from 17 mm.year^‐1 at the mountain ranges to 6 mm.year^‐1 in the plain, whereas the mean annual rainfall is about 300 mm. In response to the increase of agricultural activity, recharge fluxes to the plain were amplified and nowadays reach up to 210 mm.year^‐1 in irrigated areas. These values are strengthened by global water budget and local unsaturated zone studies. Copyright © 2013 John Wiley & Sons, Ltd.     

Space–time modelling of catchment scale drought characteristics

Drought may affect all components of the water cycle and covers commonly a large part of the catchment area. This paper examines drought propagation at the catchment scale using spatially aggregated drought characteristics and illustrates the importance of catchment processes in modifying the drought signal in both time and space. Analysis is conducted using monthly time series covering the period 1961–1997 for the Pang catchment, UK. The time series include observed rainfall and groundwater recharge, head and discharge simulated by physically-based soil water and groundwater models. Drought events derived separately for each unit area and variable are combined to yield catchment scale drought characteristics. The study reveals relatively large differences in the spatial and temporal characteristics of drought for the different variables. Meteorological droughts cover frequently the whole catchment; and they are more numerous and last for a short time (1–2 months). In comparison, droughts in recharge and hydraulic head cover typically a smaller area and last longer (4–5 months). Hydraulic head and groundwater discharge exhibit similar drought characteristics, which can be expected in a groundwater fed catchment. Deficit volume is considered a robust measure of the severity of a drought event over the catchment area for all variables; whereas, duration is less sensitive, particular for rainfall. Spatial variability in drought characteristics for groundwater recharge, head and discharge are primarily controlled by catchment properties. It is recommended not to use drought area separately as a measure of drought severity at the catchment scale, rather it should be used in combination with other drought characteristics like duration and deficit volume.