Effect of Alexandrium tamarense on three bloom-forming algae

We investigated the allelopathic properties of Alexandrium tamarense (Laboar) Balech on the growth of Prorocentrum donghaiense Lu, Chattonella marina (Subrahmanyan) Hara et Chihara and Heterosigma akashiwo (Hada) Hada in a laboratory experiment. We examined the growth of A. tamarense, C. marina, P. donghaiense and H. Akashiwo in co-cultures and the effect of filtrates from A. tamarense cultures in various growth phases, on the three harmful algal bloom (HAB)-forming algae. In co-cultures with A. tamarense, both C. marina and H. akashiwo were dramatically suppressed at high cell densities; in contrast, the growth of P. donghaiense varied in different inoculative ratios of A. tamarense and P. donghaiense. When the ratio was 1:1 (P. donghaiense: A. tamarense), growth of P. donghaiense was inhibited considerably, while the growth of P. donghaiense was almost the same as that of the control when the ratio was 9:1. The growth difference of P. donghaiense, C. marina and H. akashiwo when co-cultured with A. tamarense indicated that the allelopathic effect may be one of the important factors in algal competition and phytoplankton succession involving A. tamarense. In addition, the filtrate from A. tamarense culture had negative impacts on these three HAB algae, and such inhibition varied with different growth phases of A. tamarense in parallel with reported values of PSP toxin content in Alexandrium cells. This implied that PSP toxin was possibly involved in allelopathy of A. tamarense. However, the rapid decomposition and inactivation of PSP toxin above pH 7 weakened this possibility. Further studies on the allelochemicals responsible for the allelopathy of A. tamarense need to be carried out in future.     

Effects of water depth and substrate color on the growth and body color of the red sea cucumber,Apostichopus japonicus

Three color variants of the sea cucumber,Apostichopus japonicus are recognized,the red one is highly valued in the market. When the red variant is cultured in ponds in China,its body color changes from red to celadon in 3–6 months. The effects of water depth and substrate color on the growth and body color of this animal were investigated. Juveniles of red A. japonicus were cultured in cages suspended at a range of water depths(20,50,100,150 and 200 cm). The specific growth rate of red sea cucumbers was significantly higher in animals cultured at deeper water layers compared with those grown at shallowers. Body weights were greatest for sea cucumbers cultured at a depth of 150 cm and their survival rates were highest at a depth of 200 cm. A scale to evaluate the color of red sea cucumbers(R value) was developed using a Pantone standard color card. All stocked animals in the 9-month trial retained a red color,however the red body color was much more intense in sea cucumbers cultured at shallower depths,while animals suspended in deeper layers became pale. In a separate trial,A. japonicus were cultured in suspended cages with seven different colored substrates. Substrate color had a significant effect on the growth and body-color of red A. japonicus. The yield were greatest for A. japonicus cultured on a yellow substrate,followed by green white orange red black and blue. All sea cucumbers in the 7-month trial retained a red color,although the red was most intense(highest R value) in animals cultured on a blue substrate and pale(lowest R value) for animals cultured on a green substrate.     

Effect of nitrogen to phosphorus ratios on cell proliferation in marine micro algae

The ratio of nitrogen/phosphorus (N/P) is known to affect cell proliferation of some marine micro algae. We evaluated the effect of N/P ratios on the proliferation and succession of phytoplankton using five marine micro algae species. We used two sources of nitrogen, NH₄Cl (N₁) and urea (N₂), and a single source of phosphorous, NaH₂PO₄(P). The optimal N/P ratio that differed among the five species was affected by the source of nitrogen, being as follows (N₁/P, N₂/P in order): Thalassiosira sp. (30/1, 20/1), Heterosigma akashiwo (30/1, 30/1), Chroomonas salina (20/1, 30/1), Chaetoceros gracilis (40/1, 60/1), and Alexandrium sp. (10/1, 30/1). Thus, the source of nitrogen must be considered when analyzing the N/P ratio. Our results provide insight for predicting phytoplankton succession in coastal waters and may be used to forecast the potential risk of harmful algal blooms.     

Effect of nitrogen to phosphorus ratios on cell proliferation in marine micro algae

The ratio of nitrogen/phosphorus (N/P) is known to affect cell proliferation of some marine micro algae. We evaluated the effect of N/P ratios on the proliferation and succession of phytoplankton using five marine micro algae species. We used two sources of nitrogen, NH4Cl (N1) and urea (N2), and a single source of phosphorous, NaH2PO4(P). The optimal N/P ratio that differed among the five species was affected by the source of nitrogen, being as follows (N1/P, N2/P in order): Thalassiosira sp. (30/1, 20/1),...     

The absorption of water color components and spectral modes in the Pearl River estuary

In-situ data from cruises in the Pearl River estuary and adjacent marine areas were collected during March to May 2001. The absorption coefficients of the water color components were studied in detail containing total suspended matter (TSM), chlorophyll-a (chl-a), colored dissolved organic matter (CDOM), and de-pigment particles. For absorption coefficient of TSM, ap, and that of de-pigment particles, ad, correlations of ap(440)-TSM, ad(440)-TSM, ap(440)-chl-a and ad-chl-a were done (the italicized term means the concentration). There was a good correlation between ap(440) and chl-a concentration. An empirical relationship model between aph(675) and chl-a was developed showing a strong correlation of 0.93. Based on the two models the chl-a and aph(λ) were correlated. The values of calculated empirical spectral slope for CDOM absorption coefficients and that of de-pigment particles, 0.017 0 and 0.011 6 respectively, both are within a relative standard error of 10.0%.     

Study on algae removal by immobilized biosystem on sponge

In this study, sponges were used to immobilize domesticated sludge microbes in a limited space, forming an immobilized biosystem capable of algae and microcystins removal. The removal effects on algae, microcystins and UV260 of this biosystem and the mechanism of algae removal were studied. The results showed that active sludge from sewage treatment plants was able to remove algae from a eutrophic lake＇s water after 7 d of domestication. The removal efficiency for algae, organic matter and microcystins increased when the domesticated sludge was immobilized on sponges. When the hydraulic retention time （HRT） was 5h, the removal rates of algae, microcystins and UV260 were 90%, 94.17% and 84%, respectively. The immobilized biosystem consisted mostly of bacteria, the Ciliata and Sarcodina protozoans and the Rotifer metazoans. Algal decomposition by zoogloea bacteria and preyingby microcreatures were the two main modes of algal removal, which occurred in two steps： first, absorption by the zoogloea; second, decomposition by the zoogloea bacteria and the predacity of the microcreatures.     

Temperature and silicate are significant driving factors for the seasonal shift of dominant diatoms in a drinking water reservoir

The individual or combined ef fect of water temperature and silicate on seasonal shift of dominant diatom species in a drinking water reservoir in China was studied in this paper. These ef fects were analyzed based on the field investigation of temporal dynamics in species composition and abundance of phytoplankton and environmental factors from September 2015 to August 2016. We firstly found that six dominant diatom species( Fragilaria nanana, Achnanthidium catenatum, Aulacoseira ambigua, Ulnaria ulna, Cyclotella meneghiniana and Asterionella formosa(Class Bacillariophyceae)), which accounted for98.7% of the total abundance of diatoms and 46.8% of the total abundance of phytoplankton, showed an obvious seasonal succession. Then significant driving factors for seasonal shift of the dominant diatom species were selected by Redundancy Analysis. The result showed that water temperature and silicate were the main environmental factors af fecting the growth of diatoms on temporal scales. Next, the regressions of water temperature and silicate and dominant diatom abundance were fitted in Generalized Additive Model separately, and the smoothers of water temperature and diatom species suggested that the dominant diatom species adapted to dif ferent optimum temperature ranges, which corresponded with the growth of seasonal changes. A positive linear correlation between silicate and diatom abundance was generated by Generalized Additive Model. Finally, the ordinal controls of water temperature and silicate on the growth of diatoms were analyzed on temporal scales specifically. We suggested that water temperature and silicate controlling the growth of diatoms in order. Diatoms grow well only when the two controlling factors simultaneously satisfy the growth conditions; as limiting factors, the two factors played their respective limiting roles in turn on temporal scales.     

Effect of water depth on wind-wave frequency spectrum II. Verification and comparison

The spectrum derived in Part I of the present paper is here systematically verified with field data and compared at some length with that obtained by multiplying the deep-water spectrum with the Kitaigorodskii factor. Project supported by the National Natural Science Foundation of China.     

BIOCONCENTRATION OF TETRACHLOROBENZENE IN MARINE ALGAE

INTRODUCTIONChlrinatal~arewidelyedinindusnyandhouseholds,andsoisofsboi-ficantenviID~lcom,hauseitisoftenndaswaste.contributingtothepoilutantsinalltyPesofwater(Afghanetal.,l979).AlthoughsornedataontoxidtyandbioconnitionofchiorinatalboinaquaticorganismSwerepublishedopbasetal.,l987,LohnerCtal.,l987,Maunot,l987,Kuhnctal.,l99O),thebioCOntritionpotritinlparade,indudingk,,k,andBCF,werenotdetenninWelldriusethernehodsford~ngtheparaIneterswereleSsthanaccurate(Wangetal.,l996).Thepresentstudyab…     

Study on the dimethylsulfide and dimethylsulfoni-opropionate in algae by laboratory batch cultures

INTRODUCTIONDimethylsulfide(DMS)anditsprecursordimethylsulfoniopropionate(DMSP)areofbiogenicorigin .DMSandDMSPconcentrationsshownoclearcorrelationwithplanktonbiomassorproductivityinsomesearegions.ThismaybeduetothefactthatfactorslikephytoplanktonspeciationandphysiologicalstatearemoreimportantthanalgalbiomassorproductivityincontrollingDMSlevelsinseawater (Barnardetal.,1 984 ) .TurnerandMalin (1 988)andAndreae (1 990 )suggestedthattheunderlyingreasonfortherelativelyhighabundancesofDM…     

海南省三亚海藻资源调查

通过对海南島三亚海藻的分布和种类进行深入调查,采集和整理了标本123号,计35属53种,并分析了三亚的气候和环境条件对海藻的生长与分布的关系,为三亚海藻的开发与利用提供了第一手资料。     

Influence of dimethylsulfide produced by marine algae on the global climate

DMS (dimethylsulfide), a breakdown product of cellular solutes of many species of macroalgae andphytoplankton plays an important role in regulating global climate and counteracting partly the "greenhouse" effect.In this paper, the advance and prospects of DMS study are reviewed and discussed with respectto DMS sample storage, measurement and importance in regulating global climate and the acidity ofrain and aerosol.     

EFFECT OF WATER DEPTH ON WIND-WAVE FREQUENCY SPECTRUM Ⅰ.SPECTRAL FORM

Wen et al.'s odhed dewiogh to obtain wind-wave frequency spectrum in deep was used toderive the spectrum in finite depth water. The spedrum S(ω) (ω bein angular frequency) when normalizedwith the zeroth moment m and peak frequercyω。 contains in adrition to the peakness factor P=ω。S(ω。)/m。, a twth parameter n=(2πm。)_(1/2)d (d being water depPth), so the spatrum behavior can bestudies for different ware growth stages and water depths.     

TOXICITY OF CHLORINATED BENZENES TO MARINE ALGAE

Towhomcorrespondenceshouldbeaddressed.ImODUcr0NMarinealgaearethemosttwortantprimaryproducersandplayanimPortantroleindetenniningthefateofpersistenthydrophobicorganicchdricaIs(HOCs)inthernarineeresySteIn.TheirhydrophobicnatureresultsinanenhanedassociationwithH0Csintheanwatercolurnn(Swackhameretal.,l993).HOCfateisdowhnatalbytwoproanes,verti-caltransporttobottomsedimentSbydeadCellsandtIansportintothefood-webbyrooplanktongraring.TherelativetoortanceofthetwoproassesdependsonprharyproduCtion…     

考虑含水率对人工冻结红黏土力学特性的影响

在冻结法施工中,冻土的强度及变形特性对冻结壁的稳定性至关重要。为了研究不同含水率对冻结作用下土的强度和蠕变的影响,以江西红黏土为研究对象,通过冻结三轴试验,在-10℃下研究含水率对冻结红黏土强度及蠕变特征的影响。试验结果表明：16%～32%含水率范围内,冻结红黏土的抗压强度随含水率的增加先增大后减小;16%～28%含水率范围内,随着含水率增大,其黏聚力逐渐增大,内摩擦角逐渐减小;围压为0.2 MPa与0.5 MPa下的蠕变曲线都显示含水率较低时只会出现衰减蠕变阶段和稳定蠕变阶段,含水率较高时会出现加速蠕变阶段。含水率对红黏土的力学性质影响较大,研究成果可为江西地区红黏土地层地铁隧道建设人工冻结法施工提供参考。     

Effect of climate change on seasonal water use efficiency in subalpine Abies fabri

Abies fabri is a typical subalpine dark coniferous forest in southwestern China. Air temperature increases more at high elevation areas than that at low elevation areas in mountainous regions,and climate change ratio is also uneven in different seasons. Carbon gain and the response of water use efficiency(WUE) to annual and seasonal increases in temperature with or without CO_2 fertilization were simulated in Abies fabri using the atmospheric-vegetation interaction model(AVIM2). Four future climate scenarios(RCP2.6,RCP4.5,RCP6.0 and RCP8.5) from the Coupled Model Intercomparison Project Phase 5(CMIP5) were selectively investigated. The results showed that warmer temperatures have negative effects on gross primary production(GPP) and net primary production(NPP) in growing seasons and positive effects in dormant seasons due to the variation in the leaf area index. Warmer temperatures tend to generate lower canopy WUE and higher ecosystem WUE in Abies fabri. However,warmer temperature together with rising CO_2 concentrations significantlyincrease the GPP and NPP in both growing and dormant seasons and enhance WUE in annual and dormant seasons because of the higher leaf area index(LAI) and soil temperature. The comparison of the simulated results with and without CO_2 fertilization shows that CO_2 has the potential to partially alleviate the adverse effects of climate warming on carbon gain and WUE in subalpine coniferous forests.     

Genetic diversity analysis with ISSR PCR on green algae Chlorella vulgaris and Chlorella pyrenoidosa

In the present study, genetic polymorphism and diversity in unicellular clones of Chlorella vulgaris Beijerinck and Chlorella pyrenoidosa Chick were studied with Inter Simple Sequence Repeats PCR (ISSR PCR). Samples including four clones of C. vulgaris and three clones of C. pyrenoidosa were purified by single-clone-choice method. For four C. vulgaris unicellular clones, the total number of the bands scored for 18 primers was 298; and the number of the polymorphic bands was 118, of which 39.6% were polymorphic. The size of PCR products ranged from 200 to 2 500 bp. The total number of bands scored for 18 primers, the number of polymorphic bands and the percentage of three C. pyrenoidosa unicellular clones was 194.83 and 30.8%, respectively. POPGENE analysis show that the average Nei genetic diversity (h *) and Shannon index of diversity (I *) in the four C. vulgaris unicellular clones was 0.2181 and 0.3208, respectively, which is slightly higher than those of the three C. pyrenoidosa unicellular clones (0.190 3 and 0.274 8), which agreed with the percentage of polymorphic bands in the mixed samples of the two species. The results suggest that ISSR is a useful method to Chlorella for intra-species genetic analysis.     

Effect of slope gradient on the subsurface water flow velocity of sand layer profile

Subsurface water flow velocity influences the hydrodynamic characteristics of soil seepage and the interaction between subsurface water flow and surface runoff during soil erosion and sediment transport. A visualized method and equipment was adopted in this study to observe the subsurface water flow. Quartz sand was used as the test material of subsurface water flow and fluorescent dye was used as the indicator for tracing subsurface water flow. Water was supplied at the same flow discharge to the three parts at the bottom of the test flume, and the subsurface water flow were determined with four slope gradients(4°, 8°, 10°, and 12°). The results showed that the seepage velocity gradually increased with increasing slope gradient. The pore water velocity at different depths of sand layer profile increased with increasing slope gradient, whereas the thickness of the flow front gradually decreased. For the same slope gradient, the pore water velocity in the lower layer was the largest, whereas the thickness of the flow front was the smallest. Comparative analysis of the relationship between seepage velocity and pore water velocity at different depths of sand layer profile showed that the maximum relative difference between the measured pore water velocity and the computational pore water velocity at different depths of sand profile in the experiment was 4.38%. Thus,the test method for measuring the subsurface water flow velocity of sand layer profile adopted in this study was effective and feasible. The development of this experiment and the exploration of research methods would lay a good test foundation for future studies on the variation law of subsurface water flow velocity and the determination of flow velocity in purple soils, thus contributing to the improvement of the hydrodynamic mechanism of purple soils.     

IDENTIFICATION OF CYTOKININ IN A GREEN ALGAE EXTRACT

Isopentnyladenosine(i~6Ado) was identified, and trans-zeatin (trans-Z) and trans-zeatin riboside(trans-ZR) were detected by high pressure liquld chrmoatoguphy (HPLC) but not verified withchromatography - mass spectrometry (GC - M S ) analysis of cytokinin from the extracts of green algae (Ulvapertusa (Kjellm), Enteromopha compressa and Monostma sp). This indicated that the green algae mixturecontained cytokinin -like substances.