Purine and pyrimidine bases in marine particles in the Seto Inland Sea, Japan

Purine and pyrimidine bases in marine environmental particles collected in Harima-Nada, the Seto Inland Sea, Japan, were investigated by high performance liquid chromatography.Purines and pyrimidines concentrations varied from 0.3 to 9.3 μg l⁻¹ (n=20) for suspended matter, and 0.3 to 0.6 mg g⁻¹ (n=10) for sinking particles. A good correlation was found between chlorophyll a and purine+pyrimidine bases in suspended matter, indicating that these bases contained in suspended matter originated from phytoplankton.A comparison between several compositional data of the suspended matter and the sinking particles, namely CN ratio, composition of purines and pyrimidines, and percentages of the nitrogen bases relative to total particulate organic nitrogen, demonstrates that the sinking particles were different from suspended matter. Also, from the variety of purine and pyrimidine concentrations of marine particle samples, it was estimated that the decomposition rate of these bases seemed more rapid than decomposition rates of amino acids reported in our earlier study.     

Some features of winter convection in the Japan Sea

Historical Russian data provide indication of winter convection reaching down to about 1000 m depth near Vladivostok. However, this kind of convection does not occur every winter. Further data analysis suggests that the location of convection is driven offshore by the coastal buoyant water, which otherwise would be the coastal area. The coastal buoyant water is mostly cold fresh water but occasionally warm coastal water in the south. Due to the large extent of fresh coastal water in the northern part of the basin, the convection does not happen in this area despite the most intense surface cooling.     

Carbon dioxide in surface seawaters of the Seto Inland Sea,Japan

Observations were made of time variations of carbon dioxide in seawater, pCO₂, and in the atmosphere, PCO₂, in the Seto Inland Sea of Japan. The pCO₂ data showed well defined diurnal variation; high values at nighttime and low values during daylight hours. The pCO₂ correlated negatively with dissolved oxygen. These results denote that the diurnal variation of pCO₂ is associated with effects of photoplankton's activity in seawater. The pCO₂ measured in the Seto Inland Sea showed higher values than the PCO₂ during June to November, denoting transport of carbon dioxide from the sea surface to the atmosphere, and lower values during December to May, denoting transport of carbon dioxide from the atmosphere to the sea surface. The exchange rates of carbon dioxide were calculated using working formula given by Andriéet al. (1986). The results showed that the Seto Inland Sea gained carbon dioxide of 1.0 m-mol m^–2 d^–1 from the atmosphere in March and lost 1.7 m-mol m^–2 d^–1 to the atmosphere in August.     

A numerical study of the seasonal circulation in the Seto Inland Sea,Japan

The seasonal variation of water circulation in the Seto Inland Sea is investigated using a high resolution, three-dimensional numerical ocean model. The model results are assessed by comparison with long-term mean surface current and hydrographic data. The simulated model results are consistent with observations, showing a distinct summer and winter circulation patterns. In summer the sea water is highly stratified in basin regions, while it is well mixed near the straits due to strong tidal mixing there. During this period, a cold dome is formed in several basins, setting up stable cyclonic eddies. The cyclonic circulation associated with the cold dome develops from May and disappears in autumn when the surface cooling starts. The experiment without freshwater input shows that a basin-scale estuarine circulation coexists with cyclonic eddy in summer. The former becomes dominant in autumn circulation after the cold dome disappears. In winter the water is vertically well mixed, and the winter winds play a significant role in the circulation. The northwesterly winds induce upwind (downwind) currents over the deep (shallow) water, forming a “double-gyre pattern” in the Suo-Nada, two cyclonic eddies in Hiuchi-Nada, and anticyclonic circulation in Harima-Nada in vertically averaged current fields.     

Open Ocean Originated Phosphorus and Nitrogen in the Seto Inland Sea,Japan

The ratios of phosphorus and nitrogen originating from land and the open ocean in the Seto Inland Sea, which is the largest semi-enclosed coastal sea in Japan, have been estimated from data on total phosphorus and nitrogen loads and observed concentrations of total phosphorus and nitrogen. The ratios of land-origin and open-ocean-origin total phosphorus and nitrogen in the Seto Inland Sea are 0.28:0.72 and 0.19:0.81, respectively.     

Measurements of carbon dioxide in the Seto Inland Sea of Japan

The carbon dioxide in seawater (pCO₂) was measured in the Seto Inland Sea of Japan using newly developed equilibrator instrument designed to be free from the correction for addition or extraction of the carbon dioxide from carrier gas. The temperature dependence of pCO₂ was about 4.5%pCO₂/°C for a single seawater sample which was processed as free from biological activity and change in total carbon dioxide content during an experiment. The decrease in pCO₂ during daylight hours due to the photosynthetic fixation was about 30% of the daily mean of pCO₂ during warm months and about 15% during cold months. The effect of carbon dioxide exchange between air and seawater on pCO₂ was about 0.6 ppm in August and about 0.1 ppm in March. This is negligible small compared with the daily oscillation of carbon dioxide in seawater.     

Long-term (36-year) observations on the dynamics of the fish-killing raphidophyte Chattonella in Harima-Nada,eastern Seto Inland Sea,Japan

Long-term changes of the fish-killing raphidophyte Chattonella spp. (Chattonella antiqua, Chattonella marina and Chattonella ovata) were examined in relation to environmental factors at 19 sampling stations in Harima-Nada, eastern Seto Inland Sea, Japan, for 36 years from 1973 to 2008. Long-term trends in the dynamics of Chattonella populations were considered to relate to environmental factors such as nutrient concentrations and water temperature. High nutrient levels during the period from the 1970s to the early 1980s have contributed to the high cell density and large-scale red tides of Chattonella spp. in Harima-Nada. However, nutrient levels exhibited a decreasing trend thereafter, and it is thought that Chattonella spp. cannot form large-scale blooms under the present conditions. After the mid-1990s, the occurrence period of vegetative cells of Chattonella spp. has been several weeks or 1 month earlier than that of the 1970s and early 1980s, and the appearance frequency of Chattonella spp. has increased in the northern coastal area, although the cell density and the spatial scale of the distribution have become lower and smaller than those in the previous decades. It is suggested that the timing of germination of Chattonella cysts has become earlier as a result of the increase in water temperature, and the chances of vegetative growth have also increased, especially at the northern coast where most of large rivers discharge into the Harima-Nada. In addition, the present results revealed that fewer diatoms were also one of the significant factors for the high abundance of Chattonella spp. in Harima-Nada.     

Phosphorus content of zooplankton from the Inland Sea of Japan

Measurements of phosphorus content were made for eleven species of copepods, two species of mysids, shrimps (including mysis stage) and chaetognaths, and one species ofNoctiluca, cirriped (naupliar stage) and crab (zoeal stage) from the Inland Sea of Japan. The body phosphorus content (P, inµg) is highly correlated to prosome length (L, inµm) for copepods, which is expressed as logP=2.90 ×logL–9.34. Mean phosphorus content expressed as percent of dry weight is 1.30% for copepods, 1.39% for mysids, 0.63% for a decapod shrimpAcetes japonicus, and 0.80% for chaetognaths.Noctiluca contains phosphorus of only 0.36%. The comparatively higher phosphorus content of suspension-feeding copepods may be related to the constant supply of phosphorus-rich phytoplankton in this region.     

Mass balance of heavy metals in the seto inland sea, Japan

To discuss the geochemical and environmental behavior of heavy metals in the Seto Inland Sea, the largest semi-enclosed coastal sea in Japan, mass balances of Cu and Zn were studied by application of a simple box model using sedimentation rates and heavy metal contents of core sediments. In 1980, total sedimentary loads of Cu and Zn over the whole area of the Sea were estimated to be 630 and 3500 tons year⁻¹, respectively. Further, the sedimentary loads without (natural) and with human activities were estimated separately to be, respectively, 320 and 310 tons year⁻¹ for Cu and 1800 and 1700 tons year⁻¹ for Zn. Total inputs of Cu and Zn into the Sea, in 1980 were estimated to be 870 and 4250 tons year⁻¹ about one half of this being the result of human activities. 70% of the Cu input and 80% of the Zn input are taken into the sediments. Mean residence times of Cu and Zn in the Sea are calculated to be ≈0.3 and 0.2 years, respectively. Since these values are relatively small compared to the mean residence time of the seawater (0.9 years), Cu and Zn supplied to the Sea are considered to be accumulated rapidly in the sediments.     

Length-weight relationships of important zooplankton from the Inland Sea of Japan

Measurements of dry weight, carbon- and nitrogen contents together with the body length of important zooplankton from the Inland Sea of Japan were made using freshly caught specimens. The values of the former three parameters were found to be highly correlated to length, and species specific regression equations were calculated for 10 species of Copepoda (Calanus sinicus, Euchaeta plana, E. concinna, Centropages abdominalis, Sinocalanus tenellus, Acartia clausi, A. tsuensis, Tortanus forcipatus, Oithona brevicornis andO. similis), 3 species of Cladocera (Podon leuckarti, P. polyphemoides andPenilia avirostris), 1 species of each of Mysidacea (Neomysis japonica), and Natantia (Acetes japonicus), and two forms of Chaetognatha (Sagitta crassa andS. crassa f.naikaiensis).     

The role of Nuta (large amorphous particles) as a nutrient regenerator in Osaka Bay

Large amorphous particles (Nuta) observed in coastal areas after phytoplankton blooms and red tide outbreaks were collected by Nuta traps. These particles are always thickly attached to mooring ropes and/or fishing nets. From the decomposition experiments of Nuta and the sinking particles, dissolved inorganic nitrogen (DIN) and dissolved inorganic phosphorus (DIP) regenerations were active in Nuta, but were not active in sinking particles. In Nuta, regeneration abundances during 10 days were 567 g-N/mg of initial particulate organic nitrogen (PON), and 583 -P/mg of initial particulate phosphorus (PP), respectively. Thus DIP was more regenerated from Nuta than DIN. Ten days integral regeneration abundances of DIN and DIP from Nuta were estimated to be 36% and 79% of in situ DIN and DIP standing stocks, respectively. Nuta contributes an important role of nutrient regeneration particularly DIN, in coastal water. Carbon and nitrogen stable isotope ratios of suspended particles, sinking particles and Nuta indicated that these three different type of particles were almost the same origin, and thus Nuta in the coastal water should be made from phytoplankton debris.     

Some characteristic features of the vertical profile of organic matter in recent sediment from the Bering Sea

Sedimentary samples were collected from a site (57°02.9N, 176°57.4W: 3,650 m) in the Aleutian Basin of the Bering Sea and analyzed for organic carbon, total nitrogen and various organic materials. Organic carbon and total nitrogen were measured in the range of 0.1–1.9% and 0.01–0.2% of the dry weight of the sediment, these values tended to decrease with depth, but considerably lower values were obtained in the volcanogenic sediment layers. Carbohydrate, amino acid and protein and lipid carbons accounted for 40% of the total organic carbon on the surface of the sediment, and this value tended to decrease with depth to 20% at 10m depth from the top of the core sample. The carbon to nitrogen (C/N) ratios ranged from 6.3 to 9.0. Vertical change in the values of the ratios can be understood in terms of a higher decay rate of amino acids and proteins.Radiocarbon age determinations on five sedimentary samples yielded sedimentation rates ranging from 37 to 90cm/1,000y.     

Heavy metals and accumulation rates of sediments in Osaka Bay,the Seto Inland Sea,Japan

The metal load into sediments and the change in the sedimentary environment of Osaka Bay in the Seto Inland Sea have been studied through geochemical analysis of core sediments, using both Pb-210 dating and a selective chemical leaching technique. Analytical results from a 6-m core of sediment show that copper and zinc pollution started in the late 1800's and the present enrichment ratios of copper and zinc, relative to background levels (20 mg kg^–1 for Cu and 94 mg kg^–1 for Zn), are 2.8 and 4.1, respectively. The present anthropogenic copper and zinc loads into Osaka Bay sediments, are 47 and 368 ton yr^–1, while natural copper and zinc loads are 40 and 186 ton yr^–1, respectively. Osaka Bay sediment at the present day is considered to be seriously polluted by zinc, now. The vertical profiles of copper and zinc in four successively separated fractions (10% acetic acid soluble fraction: F-HAC, 0.1M hydrochloric acid-soluble fraction: F-HCl, hydrogen peroxide-soluble fraction: F-H₂O₂ and hydrofluoric acid-soluble fraction: F-HF) from the core sediments indicate that enrichments of copper and zinc in the upper layer of the sediment are dependent on increases in the metal contents of the F-HAC, F-HCl and F-H₂O₂ fractions. Copper in F-HAC, and zinc in F-HAC and F-HCl, seem to be of anthropogenic origin.Results of sequential studies of the whole Seto Inland Sea can be summarized as follows: At the present time, the sedimentary loads of copper and zinc over the whole Seto Inland Sea area are 630 and 3,500 ton yr^–1, respectively, while the natural and anthropogenic loads are 320 and 310 ton yr^–1 for copper and 1,800 and 1,700 ton yr^–1 for zinc, respectively.     

Reciprocal sound transmission measurement of mean current and temperature variations in the central part (Aki-nada) of the Seto Inland Sea,Japan

A 30 km-range reciprocal sound transmission experiment was carried out on the line connecting Honshu and Shikoku (the first and fourth biggest main Japanese islands, respectively) in the central part (Aki-nada) of the Seto Inland Sea, Japan, during March–May 2010 to measure the mean current and temperature variations over the sea. The range-averaged current along the sound transmission line was estimated to have a mean and standard deviation of (3.8–4.4) ± (1.7–1.8) cm/s after converting the travel time difference data into currents, including a fortnightly tidal variation in the range of ±30 cm/s. The positive mean current implies slow water movement from the west to east through Aki-nada. The range-averaged speed of sound was estimated by converting from the mean travel time or one-way travel time into the speed of sound, and further converted into temperature for fixed values of salinity and depth, according to the standard speed of sound formula. Besides the precise measurement (to an accuracy of 0.01°C) of semidiurnal and diurnal tidal variations and seasonal warming, the temperature data showed periodic variations with periods of 7.0 and 21.1 days that had never been observed in Aki-nada before. This study suggests that reciprocal sound transmission is a powerful technique for the long-term accurate measurement of mean current and temperature variations in coastal and inland seas.     

Analysis of primary production in the Seto Inland Sea, Japan, using a simple ecosystem model

A simple model of lower trophic level ecosystem has been created to analyze possible environmental control of primary production in eight sub-areas of the Seto Inland Sea. The primary production rates observed by Hashimotoet al. (1997a) in these sub-areas are well reproduced by the model, including horizontal processes such as horizontal transport of nutrients and vertical processes such as vertical mixing, light intensity and sinking of particulate matter. Without taking account of horizontal processes the model also successfully reproduces the observed primary production rates in some areas, but if fails to reproduce those in the others. This shows that the relative importance of the horizontal transport on the primary production differs are by area. Two time scales,T _z andT _H, are introduced to explain this difference.T _z is a vertical cycling time of material, which is defined as the time during which the stock of the material in the water column is utilized for primary production;T _H is the horizontal transit time of the material. The relative importance of the horizontal process is well explained by theT _H/T _z ratio; that is, the horizontal transport process is important in the areas where this ratio is small. Further the possible mechanisms of nutrient supply for the primary production in each sub-area are investigated using this model.     

Evidence of microphytobenthic roles on coastal shallow water of the Seto Inland Sea,Japan

This study describes the temporal variation of microphytobenthic biomass and its controlling factors, as well as the impact of microphytobenthic activities on coastal shallow sediment in the eastern Seto Inland Sea, Japan. The chlorophyll a (Chl a), phaeopigments and sedimentary biophilic element (C, N, P and Si) contents in surface sediments, as well as nutrient concentrations at the sediment-water interface (overlying water and pore water) were observed monthly during November 2003 to May 2005 at one site in Shido Bay (water depth ca. 7 m) and at one site in Harima-Nada (35 m). No correlation was observed between the sedimentary biophilic elements and other parameters. The maximum chlorophyll a content of 48.2 μg g^–1 was found in surface sediments under the photon flux reaching the seafloor of 537 μmol photon m^–2 s^–1 during the winter period when water transparency was the highest at station S (Shido Bay). Our results suggest that higher chlorophyll a content in surface sediment was due to the fresh microphytobenthic biomass (mainly benthic diatom). We also found a significant negative correlation between Chl a and inorganic nutrients in pore water during the low temperature period, especially silicic acid. This result suggests that the silicic acid was assimilated largely during the increase of microphytobenthic biomass in surface sediment. This study suggests that the microphytobenthic roles may have a great effect on nutrient budgets during the large supply of irradiance (winter periods) for their photosynthetic growth in shallow coastal waters.     

Dynamics of microphytobenthic biomass in a coastal area of western Seto Inland Sea, Japan

This study focused on the causes of the variation in microphytobenthic biomass and the effects of this variation on macrobenthic animals in the western Seto Inland Sea, Japan, where the importance of microphytobenthos as the primary food source for benthic animals has been recently reported. We investigated the microphytobenthic biomass together with light attenuation of seawater, phytoplanktonic biomass, macrobenthic density and biomass at eight stations (water depth = 5–15 m) during four cruises in 1999–2000. The increased light attenuation coefficient of the water column associated with increased concentration of the phytoplanktonic Chl-a caused a decrease in light flux that reached the seafloor. The biomass of the microphytobenthos within the upper 1 cm of the sediment, 1.9–46.5 mg Chl-a m⁻², was inversely correlated with the phytoplanktonic biomass in the overlying water column, 10.9–65.0 mg Chl-a m⁻². Thus, interception of light by phytoplankton is considered to be a main cause of the variation in the microphytobenthic biomass. The microphytobenthos biomass showed a significant positive correlation with the macrobenthic density (78–9369 ind. m⁻²) and biomass (0.4–78.8 gWW m⁻²). It appears that the increase in oxygen production by the microphytobenthos allowed macrobenthic animals to become more abundant, as a consequence of oxygenation of the organically enriched muddy sediments (14.5 ± 2.69 mg TOC g⁻¹). This study suggests that the variation in the microphytobenthic biomass is influenced by the phytoplanktonic biomass due to shading effect, and the balance between these two functional groups might affect the variability in the macrobenthic density and biomass.     

Fluctuations of the calcite compensation depth (CCD) in the East Sea (Sea of Japan)

Variations in the calcite compensation depth (CCD) in the East Sea (Sea of Japan) since the early middle Miocene were inferred from data on age-depth relationships, sediment carbonate, and benthic foraminifers from ODP sites 794, 795, and 797. The CCD remained relatively shallow during much of the middle Miocene–Pliocene, and deepened sharply at the beginning of the Pleistocene. Since then it has fluctuated rapidly, possibly in relation to the onset of the northern hemisphere glacial cycles in the late Pliocene. The average CCD has deepened since at least the early middle Miocene, coinciding with the long-term drop in eustatic sea level. Received: 5 November 1998 / Revision accepted: 2 February 2000     

Structure and species diversity of the zooplankton community of the Inland Sea of Japan

Seasonal fluctuations in composition and abundance of zooplankton of the Inland Sea of Japan were investigated in 1979–1980. Some of the areas in the environment were found to be eutrophic and sharp temperature variations occurred with seasons. In this scenario, zooplankton populations peaked in autumn. Copepods were the dominant component and six species of this group formed about 88% of the total zooplankton counts. Species groups are categorized based on distributional characteristics. Zooplankton composition was more or less homogeneous in each season within the area studied and a few oceanic species which occurred had only limited influence on community structure. Species diversity was low and competition among species was weak. The results indicated a poor quality biotope probably resulting from a deterioration of water quality and an environment which favours r-selection.     

南黄海环流的若干特征

主要根据近几年来中韩黄海水循环动力学合作调查结果,结合有关历史资料,对南黄海环流的若干特征进行了分析。所得主要认识为:(1)南黄海环流存在明显的季节变异。冬、夏季环流的基本形态有着较大的差别。(2)黄海暖流的路径和强度均有一定的年际变化。分析显示,1997年冬季,暖流路径明显偏于槽的西侧;而1986年冬,暖流的主流路径则沿槽北上。(3)黄海暖流并非对马暖流的直接分支。黄海暖流水是对马暖流水和陆架水混合而成。而且,它主要是在济州岛西侧海域,从锋区中衍生出来的。(4)夏季黄海表、底层环流大致皆是由一大的道时针向流系构成。但在其表层海盐尺度的气旋式环流内部还存在小的气旋和反气旋流环。分析亦表明,不论表层或底层,皆无高盐暖水从济州岛邻近海域进入黄海东部的明显迹象。